(Linear) (Six-base) MAPSORT of: KIEE3521.seq Check: 6499 from: 1 to: 1365 KIEE3521.orf With 182 enzymes: SgfI * July 3, 2012 18:03 .. AccI GT'mk_AC Cuts at: 0 435 602 1365 Size: 435 167 763 Fragments arranged by size: 763 435 167 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1333 1365 Size: 1333 32 ApaLI G'TGCA_C Cuts at: 0 1032 1365 Size: 1032 333 AvaI C'yCGr_G Cuts at: 0 236 1365 Size: 236 1129 AvrII C'CTAG_G Cuts at: 0 33 1365 Size: 33 1332 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 706 739 1365 Size: 706 33 626 Fragments arranged by size: 706 626 33 BanI G'GyrC_C Cuts at: 0 37 673 1365 Size: 37 636 692 Fragments arranged by size: 692 636 37 BanII G_rGCy'C Cuts at: 0 94 281 340 1365 Size: 94 187 59 1025 Fragments arranged by size: 1025 187 94 59 BbeI G_GCGC'C Cuts at: 0 41 1365 Size: 41 1324 BbvCI CC'TCA_GC Cuts at: 0 1335 1365 Size: 1335 30 BciVI GTATCCnnnnn_n' Cuts at: 0 308 1365 Size: 308 1057 BfrBI ATG'CAT Cuts at: 0 985 1365 Size: 985 380 Bme1580I G_kGCm'C Cuts at: 0 1036 1365 Size: 1036 329 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 108 1365 Size: 108 1257 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 370 412 623 1365 Size: 370 42 211 742 Fragments arranged by size: 742 370 211 42 Bpu10I CC'TnA_GC Cuts at: 0 1335 1365 Size: 1335 30 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 430 1365 Size: 430 935 BsaI GGTCTCn'nnnn_ Cuts at: 0 1222 1365 Size: 1222 143 BsaAI yAC'GTr Cuts at: 0 545 1365 Size: 545 820 BsaHI Gr'CG_yC Cuts at: 0 38 1365 Size: 38 1327 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 160 190 1365 Size: 160 30 1175 Fragments arranged by size: 1175 160 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 93 97 165 1365 Size: 93 4 68 1200 Fragments arranged by size: 1200 93 68 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 523 1365 Size: 523 842 BsiHKAI G_wGCw'C Cuts at: 0 94 1036 1365 Size: 94 942 329 Fragments arranged by size: 942 329 94 BsmI GAATG_Cn' Cuts at: 0 842 985 1365 Size: 842 143 380 Fragments arranged by size: 842 380 143 BsmBI CGTCTCn'nnnn_ Cuts at: 0 143 1365 Size: 143 1222 Bsp1286I G_dGCh'C Cuts at: 0 94 281 340 1036 1365 Size: 94 187 59 696 329 Fragments arranged by size: 696 329 187 94 59 BspHI T'CATG_A Cuts at: 0 317 1365 Size: 317 1048 BsrDI GCAATG_nn' Cuts at: 0 558 1365 Size: 558 807 BsrFI r'CCGG_y Cuts at: 0 468 1365 Size: 468 897 BstXI CCAn_nnnn'nTGG Cuts at: 0 1069 1365 Size: 1069 296 BstYI r'GATC_y Cuts at: 0 1064 1365 Size: 1064 301 BtgI C'CryG_G Cuts at: 0 622 1365 Size: 622 743 BtsI GCAGTG_nn' Cuts at: 0 400 1365 Size: 400 965 EaeI y'GGCC_r Cuts at: 0 257 397 854 1052 1365 Size: 257 140 457 198 313 Fragments arranged by size: 457 313 257 198 140 EarI CTCTTCn'nnn_ Cuts at: 0 82 1094 1195 1352 1365 Size: 82 1012 101 157 13 Fragments arranged by size: 1012 157 101 82 13 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 194 205 1089 1365 Size: 194 11 884 276 Fragments arranged by size: 884 276 194 11 EcoICRI GAG'CTC Cuts at: 0 92 1365 Size: 92 1273 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 370 412 623 1333 1365 Size: 370 42 211 710 32 Fragments arranged by size: 710 370 211 42 32 HaeII r_GCGC'y Cuts at: 0 41 1365 Size: 41 1324 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 118 150 428 442 460 474 889 921 Size: 118 32 278 14 18 14 415 32 Cuts at: 921 1004 1036 1365 Size: 83 32 329 Fragments arranged by size: 415 329 278 118 83 32 32 32 18 14 14 HincII GTy'rAC Cuts at: 0 715 1365 Size: 715 650 HindIII A'AGCT_T Cuts at: 0 1358 1365 Size: 1358 7 KasI G'GCGC_C Cuts at: 0 37 1365 Size: 37 1328 MscI TGG'CCA Cuts at: 0 399 1365 Size: 399 966 MspA1I CmG'CkG Cuts at: 0 9 1330 1365 Size: 9 1321 35 Fragments arranged by size: 1321 35 9 NarI GG'CG_CC Cuts at: 0 38 1365 Size: 38 1327 NdeI CA'TA_TG Cuts at: 0 806 1148 1365 Size: 806 342 217 Fragments arranged by size: 806 342 217 NsiI A_TGCA'T Cuts at: 0 987 1365 Size: 987 378 NspI r_CATG'y Cuts at: 0 1087 1365 Size: 1087 278 PvuII CAG'CTG Cuts at: 0 9 1330 1365 Size: 9 1321 35 Fragments arranged by size: 1321 35 9 SacI G_AGCT'C Cuts at: 0 94 1365 Size: 94 1271 SapI GCTCTTCn'nnn_ Cuts at: 0 82 1094 1365 Size: 82 1012 271 Fragments arranged by size: 1012 271 82 SfoI GGC'GCC Cuts at: 0 39 1365 Size: 39 1326 SmaI CCC'GGG Cuts at: 0 238 1365 Size: 238 1127 SmlI C'TyrA_G Cuts at: 0 445 1365 Size: 445 920 SphI G_CATG'C Cuts at: 0 1087 1365 Size: 1087 278 StyI C'CwwG_G Cuts at: 0 33 1068 1365 Size: 33 1035 297 Fragments arranged by size: 1035 297 33 XmaI C'CCGG_G Cuts at: 0 236 1365 Size: 236 1129 Enzymes that do cut: AccI AcuI ApaLI AvaI AvrII BaeI BanI BanII BbeI BbvCI BciVI BfrBI Bme1580I BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BsrDI BsrFI BstXI BstYI BtgI BtsI EaeI EarI EciI EcoICRI Eco57MI HaeII Hin4I HincII HindIII KasI MscI MspA1I NarI NdeI NsiI NspI PvuII SacI SapI SfoI SmaI SmlI SphI StyI XmaI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApoI AscI AseI AsiSI BamHI BbsI BcgI BclI BglI BglII BlpI BmgBI BmrI BmtI BsaBI BsaWI BseYI BsiEI BsiWI BspEI BspMI BsrBI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EagI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HpaI KpnI MfeI MluI MmeI MslI NaeI NcoI NgoMIV NheI NotI NruI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SnaBI SpeI SrfI SspI StuI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmnI ZraI