Restriction map for
pFN21AB8505
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# NO CUT ENZYMES ( 108 enzymes) #
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AatII Acc65I AccI AccII AccIII
AgeI Aor51HI AscI Asp718I AspI
AvaI AviII BamHI BanI BbeI
BbrPI BbvCI BcgI BciVI BcnI
BglII BsaAI BsaI BseAI BsiEI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstEII BstPI BstUI BstXI BstZ17I
CfoI Cfr10I ClaI CpoI DraIII
DrdI EagI EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI EcoRV FauI
FseI FspI HaeII HhaI HinP1I
KasI KpnI KspI MluI MroI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PvuI RsrII SacII
SalI SbfI SexAI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SwaI Tth111I Van91I
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AciI 2490 CCGC CCGC(-3/-1)
AcyI 2418 GGCGTC G[GA]^CG[CT]C
AflII 1806 CTTAAG C^TTAAG
AflIII 1975 ACATGT A^C[GA][CT]GT
AhdI 582 GACTTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2060 GTGCAC G^TGCAC
AlwNI 1373 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 424 CCTGAGG CC^T[ACGT]AGG
ApaI 2709 GGGCCC GGGCC^C
ApaLI 2060 GTGCAC G^TGCAC
AseI 405 ATTAAT AT^TAAT
AsnI 405 ATTAAT AT^TAAT
AspEI 582 GACTTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 2117 GGACC G^G[AT]CC
AvrII 1393 CCTAGG C^CTAGG
BalI 377 TGGCCA TGG^CCA
BclI 1947 TGATCA T^GATCA
BfrI 1806 CTTAAG C^TTAAG
BglI 1357 GCCAAAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1393 CCTAGG C^CTAGG
BlpI 663 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 663 GCTTAGC GC^T[ACGT]AGC
BsaBI 1513 GATAATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2418 GGCGTC G[GA]^CG[CT]C
BsaWI 2548 ACCGGA [AT]^CCGG[AT]
BsgI 2285 CTGCAC GTGCAG(16/14)
BsmAI 1194 GAGAC GTCTC(1/5)
BspLU11I 1975 ACATGT A^CATGT
BstBI 1844 TTCGAA TT^CGAA
Bsu36I 424 CCTGAGG CC^T[ACGT]AGG
CelII 663 GCTTAGC GC^T[ACGT]AGC
DraI 7 TTTAAA TTT^AAA
DsaI 440 CCATGG C^C[GA][CT]GG
EaeI 377 TGGCCA [CT]^GGCC[GA]
Eam1105I 582 GACTTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 424 CCTGAGG CC^T[ACGT]AGG
EcoT22I 2579 ATGCAT ATGCA^T
FbaI 1947 TGATCA T^GATCA
Hin1I 2418 GGCGTC G[GA]^CG[CT]C
HincII 2395 GTTAAC GT[CT]^[GA]AC
HindII 2395 GTTAAC GT[CT]^[GA]AC
HpaI 2395 GTTAAC GTT^AAC
MamI 1513 GATAATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 377 TGGCCA TGG^CCA
MscI 377 TGGCCA TGG^CCA
MspA1I 1661 CAGCTG C[AC]G^C[GT]G
NcoI 440 CCATGG C^CATGG
NsiI 2579 ATGCAT ATGCA^T
NspV 1844 TTCGAA TT^CGAA
PpuMI 2116 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 937 GACTTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 405 ATTAAT AT^TAAT
PstI 1306 CTGCAG CTGCA^G
PvuII 1661 CAGCTG CAG^CTG
SapI 202 GAAGAGC GCTCTTC(1/4)
ScaI 2553 AGTACT AGT^ACT
SfcI 1306 CTGCAG C^T[GA][CT]AG
SfuI 1844 TTCGAA TT^CGAA
StuI 2312 AGGCCT AGG^CCT
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# TWO CUT ENZYMES ( 19 enzymes) #
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AclI 1019 AACGTT AA^CGTT
1399 AACGTT
AcsI 339 AAATTT [GA]^AATT[CT]
1335 GAATTT
ApoI 339 AAATTT [GA]^AATT[CT]
1335 GAATTT
BpmI 385 CTGGAG CTGGAG(16/14)
2736 CTGGAG
BseRI 182 GAGGAG GAGGAG(10/8)
2068 GAGGAG
BsiYI 1238 CCCTACAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
2505 CCCAGAAATGG
BslI 1238 CCCTACAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
2505 CCCAGAAATGG
DraII 2116 GGGACCT [GA]G^G[ACGT]CC[CT]
2708 AGGGCCC
EcoO109I 2116 GGGACCT [GA]G^G[ACGT]CC[CT]
2708 AGGGCCC
HapII 1711 CCGG C^CGG
2549 CCGG
HgaI 2470 GACGC GACGC(5/10)
2419 GCGTC
HpaII 1711 CCGG C^CGG
2549 CCGG
MslI 530 CATCTATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2722 CATGAACATG
MspI 1711 CCGG C^CGG
2549 CCGG
Psp1406I 1019 AACGTT AA^CGTT
1399 AACGTT
SacI 417 GAGCTC GAGCT^C
2431 GAGCTC
Tsp45I 464 GTGAC ^GT[GC]AC
1877 GTGAC
XbaI 1578 TCTAGA T^CTAGA
2004 TCTAGA
XcmI 746 CCACGAATTATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1358 CCAAAGAGGCAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AfaI AluI AlwI Asp700I AspHI
BanII BbsI BbvI BfaI BmyI
BpuAI BsaJI BsiHKAI BsmFI Bsp1286I
BspHI BsrDI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HindIII HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MfeI MflI MnlI
MseI MunI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp509I TspRI TthHB8I
XhoII XmnI