################################## # NO CUT ENZYMES ( 63 enzymes) # ################################## Acc65I AccI AclI AcsI AflII AflIII Alw44I ApaLI ApoI AscI AseI AsnI Asp718I BamHI BcgI BclI BfrI BglII BsiWI BsmI BspDI BspLU11I BssHII Bst1107I BstBI BstZ17I ClaI CpoI DraI EcoRI EcoRV EcoT22I FbaI FseI HpaI KpnI MfeI MluI MunI NdeI NheI NotI NruI NsiI NspV PacI PmeI PshAI PshBI Psp1406I PvuI RsrII SalI ScaI SfiI SfuI SgrAI SnaBI SpeI SphI SspI SwaI XbaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AgeI 353 ACCGGT A^CCGGT AhdI 1327 GACAGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 3637 AGCGCT AGC^GCT Asp700I 1125 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 1327 GACAGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvrII 498 CCTAGG C^CTAGG BbrPI 3361 CACGTG CAC^GTG BciVI 893 GGATAC GTATCC(6/5) BlnI 498 CCTAGG C^CTAGG BlpI 942 GCTGAGC GC^T[ACGT]AGC Bpu1102I 942 GCTGAGC GC^T[ACGT]AGC BsiEI 2058 CGGCCG CG[GA][CT]^CG Bsp1407I 1109 TGTACA T^GTACA BspHI 620 TCATGA T^CATGA BsrBI 636 GAGCGG CCGCTC(-3/-3) BsrDI 275 GCAATG GCAATG(2/0) BsrGI 1109 TGTACA T^GTACA BssSI 3340 CACGAG CACGAG(-5/-1) BstEII 1528 GGTGACC G^GT[ACGT]ACC BstPI 1528 GGTGACC G^GT[ACGT]ACC CelII 942 GCTGAGC GC^T[ACGT]AGC EagI 2058 CGGCCG C^GGCCG Eam1105I 1327 GACAGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 2058 CGGCCG C^GGCCG Eco47III 3637 AGCGCT AGC^GCT Eco52I 2058 CGGCCG C^GGCCG EcoO65I 1528 GGTGACC G^GT[ACGT]ACC HindIII 2895 AAGCTT A^AGCTT KspI 1533 CCGCGG CCGC^GG MseI 1547 TTAA T^TAA NcoI 1094 CCATGG C^CATGG PaeR7I 1186 CTCGAG C^TCGAG PflMI 2622 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 353 ACCGGT A^CCGGT PmaCI 3361 CACGTG CAC^GTG PmlI 3361 CACGTG CAC^GTG RcaI 620 TCATGA T^CATGA SacI 507 GAGCTC GAGCT^C SacII 1533 CCGCGG CCGC^GG SapI 2199 GAAGAGC GCTCTTC(1/4) SbfI 2703 CCTGCAGG CCTGCA^GG SexAI 1406 ACCAGGT A^CC[AT]GGT Sse8387I 2703 CCTGCAGG CCTGCA^GG SspBI 1109 TGTACA T^GTACA TfiI 1045 GATTC G^A[AT]TC Tru9I 1547 TTAA T^TAA Tsp509I 1545 AATT ^AATT Van91I 2622 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 1186 CTCGAG C^TCGAG XmnI 1125 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AccIII 830 TCCGGA T^CCGGA 1323 TCCGGA AocI 1475 CCTCAGG CC^T[ACGT]AGG 1793 CCTCAGG AspI 394 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC 1222 GACCGTGTC BalI 2138 TGGCCA TGG^CCA 3596 TGGCCA BbeI 1774 GGCGCC GGCGC^C 3462 GGCGCC BbsI 660 GAAGAC GAAGAC(2/6) 1567 GTCTTC BfaI 499 CTAG C^TAG 1102 CTAG BpuAI 660 GAAGAC GAAGAC(2/6) 1567 GTCTTC BsaBI 418 GATGCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3030 GATCAACATC BseAI 830 TCCGGA T^CCGGA 1323 TCCGGA BspEI 830 TCCGGA T^CCGGA 1323 TCCGGA Bsu36I 1475 CCTCAGG CC^T[ACGT]AGG 1793 CCTCAGG Eco81I 1475 CCTCAGG CC^T[ACGT]AGG 1793 CCTCAGG KasI 1774 GGCGCC G^GCGCC 3462 GGCGCC MaeI 499 CTAG C^TAG 1102 CTAG MamI 418 GATGCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3030 GATCAACATC MluNI 2138 TGGCCA TGG^CCA 3596 TGGCCA MroI 830 TCCGGA T^CCGGA 1323 TCCGGA MscI 2138 TGGCCA TGG^CCA 3596 TGGCCA NaeI 3459 GCCGGC GCC^GGC 3551 GCCGGC NarI 1774 GGCGCC GG^CGCC 3462 GGCGCC NgoMIV 3459 GCCGGC G^CCGGC 3551 GCCGGC NspI 1295 ACATGC [GA]CATG^[CT] 1583 ACATGC PflFI 394 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC 1222 GACCGTGTC SfoI 1774 GGCGCC GGC^GCC 3462 GGCGCC StuI 1160 AGGCCT AGG^CCT 1784 AGGCCT Tth111I 394 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC 1222 GACCGTGTC ######################################### # 3 OR MORE CUT ENZYMES ( 118 enzymes) # ######################################### AatII AccII AciI AcyI AfaI AluI AlwI AlwNI ApaI AspHI AvaI AvaII AviII BanI BanII BbvCI BbvI BcnI BglI BmyI BpmI BsaAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DrdI DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StyI TaqI TseI Tsp45I TspRI TthHB8I XcmI XhoII XmaCI XmaI