Restriction map for
pFN21AB6494
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# NO CUT ENZYMES ( 151 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AvaII AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbsI BcgI BfaI BfrI BglI
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BsmAI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspMI
BsrBI BsrGI BssHII BssSI Bst1107I
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII CfoI ClaI CpoI
DraII DrdI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HindIII HpaI
KasI KpnI Ksp632I KspI MaeI
MamI MboII MfeI MluI MluNI
MroI MscI MunI MvnI NarI
NheI NotI NruI NsiI PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SfcI SfiI SfoI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tth111I XbaI
XhoI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AcsI 639 GAATTT [GA]^AATT[CT]
AflIII 707 ACATGT A^C[GA][CT]GT
ApoI 639 GAATTT [GA]^AATT[CT]
AspHI 539 GAGCAC G[AT]GC[AT]^C
BbvCI 527 CCTCAGC CCTCAGC(-5/-2)
BciVI 715 GTATCC GTATCC(6/5)
BclI 485 TGATCA T^GATCA
BglII 397 AGATCT A^GATCT
BseRI 33 CTCCTC GAGGAG(10/8)
BsgI 296 CTGCAC GTGCAG(16/14)
BsiHKAI 539 GAGCAC G[AT]GC[AT]^C
BsmFI 66 GTCCC GGGAC(10/14)
BspLU11I 707 ACATGT A^CATGT
BsrDI 749 GCAATG GCAATG(2/0)
BsrFI 49 GCCGGC [GA]^CCGG[CT]
BsrI 315 CCAGT ACTGG(1/-1)
BstAPI 132 GCACATTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 16 TTCGAA TT^CGAA
BstF5I 777 GGATG GGATG(2/0)
Cfr10I 49 GCCGGC [GA]^CCGG[CT]
Cfr13I 621 GGCCC G^G[ACGT]CC
DraI 790 TTTAAA TTT^AAA
DraIII 133 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 302 CCATGG C^C[GA][CT]GG
EcoT14I 302 CCATGG C^C[AT][AT]GG
FauI 88 CCCGC CCCGC(4/6)
FbaI 485 TGATCA T^GATCA
FokI 777 GGATG GGATG(9/13)
HincII 322 GTTGAC GT[CT]^[GA]AC
HindII 322 GTTGAC GT[CT]^[GA]AC
HinfI 14 GATTC G^A[ACGT]TC
HphI 464 TCACC GGTGA(8/7)
MaeII 308 ACGT A^CGT
MaeIII 220 GTCAC ^GT[ACGT]AC
MslI 222 CACTATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 49 GCCGGC GCC^GGC
NcoI 302 CCATGG C^CATGG
NdeI 371 CATATG CA^TATG
NgoMIV 49 GCCGGC G^CCGGC
NspV 16 TTCGAA TT^CGAA
PflMI 601 CCAGCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Sau96I 621 GGCCC G^G[ACGT]CC
SexAI 629 ACCAGGT A^CC[AT]GGT
SfaNI 802 GATGC GCATC(5/9)
SfuI 16 TTCGAA TT^CGAA
SmaI 69 CCCGGG CCC^GGG
StyI 302 CCATGG C^C[AT][AT]GG
TaqI 17 TCGA T^CGA
TfiI 14 GATTC G^A[AT]TC
Tsp45I 220 GTCAC ^GT[GC]AC
TthHB8I 17 TCGA T^CGA
Van91I 601 CCAGCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 467 CCACACATATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 69 CCCGGG C^CCGGG
XmaI 69 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AciI 89 CCGC CCGC(-3/-1)
53 GCGG
AfaI 146 GTAC GT^AC
502 GTAC
Asp700I 456 GAATGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
588 GAATAGCTTC
AvaI 69 CCCGGG C^[CT]CG[GA]G
122 CCCGAG
BanII 119 GAGCCC G[GA]GC[CT]^C
125 GAGCCC
BcnI 69 CCCGG CC^[GC]GG
70 CCGGG
BpmI 362 CTCCAG CTGGAG(16/14)
445 CTGGAG
BsoBI 69 CCCGGG C^[CT]CG[GA]G
122 CCCGAG
BstYI 397 AGATCT [GA]^GATC[CT]
684 GGATCT
HapII 50 CCGG C^CGG
70 CCGG
HpaII 50 CCGG C^CGG
70 CCGG
MflI 397 AGATCT [GA]^GATC[CT]
684 GGATCT
MspI 50 CCGG C^CGG
70 CCGG
NciI 69 CCCGG CC^[GC]GG
70 CCGGG
NspI 243 ACATGC [GA]CATG^[CT]
707 ACATGT
RsaI 146 GTAC GT^AC
502 GTAC
SmlI 98 CTCAAG C^T[CT][GA]AG
688 CTCAAG
Tsp509I 195 AATT ^AATT
640 AATT
XhoII 397 AGATCT [GA]^GATC[CT]
684 GGATCT
XmnI 456 GAATGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
588 GAATAGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AluI AlwI BbvI BmyI BsaJI
BsiYI BslI Bsp1286I BssKI BstNI
Cac8I DdeI DpnII EcoRII Fnu4HI
HaeIII ItaI MboI MnlI MseI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV PvuII Sau3AI ScrFI Tru9I
TseI TspRI