Restriction map for pFN21AB6494
 (Circular) (Six-base) MAPSORT of: pFN21AB6494.seq  Check: 1164  from: 1  to: 5515



pFN21AB6494  5515 bp

 With 182 enzymes: SgfI * 

                        April 15, 2010 09:23  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3325     278 
   Size:         53      83     186     936    1789    2468

  Fragments arranged by size: 

                2468    1789     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2858    1937 
   Size:        921    4594
 
Acc65I G'GTAC_C

Cuts at:   2836    2836 
   Size:       5515
 
AclI AA'CG_TT

Cuts at:   1563    3642    4015    1563 
   Size:       2079     373    3063

  Fragments arranged by size: 

                3063    2079     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2691    3578    4962    5230    1632 
   Size:       1059     887    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1059     887     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5318
 
AflIII A'CryG_T

Cuts at:   1203    2712    4414    5391    1203 
   Size:       1509    1702     977    1327

  Fragments arranged by size: 

                1702    1509    1327     977
 
AgeI A'CCGG_T

Cuts at:   4346    4346 
   Size:       5515
 
AhdI GACnn_n'nnGTC

Cuts at:   4244    4244 
   Size:       5515
 
AleI CACnn'nnGTG

Cuts at:   1166    2231    1166 
   Size:       1065    4450
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5515
 
AlwNI CAG_nnn'CTG

Cuts at:   3258    4830    3258 
   Size:       1572    3943
 
ApaLI G'TGCA_C

Cuts at:   3572    4728    3572 
   Size:       1156    4359
 
ApoI r'AATT_y

Cuts at:   1493    2644    2824    3050    3202    5311    1493 
   Size:       1151     180     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1151     226     180     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4069     160 
   Size:        872    3037    1606

  Fragments arranged by size: 

                3037    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5515
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2074    2127    2840    1870 
   Size:         73     131      53     713    4545

  Fragments arranged by size: 

                4545     713     131      73      53
 
BamHI G'GATC_C

Cuts at:   2845    3212    2845 
   Size:        367    5148
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2836    4291     618 
   Size:        325     217     627    1049    1455    1842

  Fragments arranged by size: 

                1842    1455    1049     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2128    2134    2834    1391 
   Size:        737       6     700    4072

  Fragments arranged by size: 

                4072     737     700       6
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4544
 
BbvCI CC'TCA_GC

Cuts at:   2533    2533 
   Size:       5515
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3706    3740    1903 
   Size:         34    1769      34    3678

  Fragments arranged by size: 

                3678    1769      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   2731    3407    4617    2731 
   Size:        676    1210    3629

  Fragments arranged by size: 

                3629    1210     676
 
BclI T'GATC_A

Cuts at:   1548    2490    1548 
   Size:        942    4573
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4126     136 
   Size:        107     122      71     631     605    2454    1525

  Fragments arranged by size: 

                2454    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   2402    5510    2402 
   Size:       3108    2407
 
BlpI GC'TnA_GC

Cuts at:   2932    2932 
   Size:       5515
 
Bme1580I G_kGCm'C

Cuts at:   1790    2148    2766    3576    4732    1790 
   Size:        358     618     810    1156    2573

  Fragments arranged by size: 

                2573    1156     810     618     358
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5515
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3246    4204     442 
   Size:        416      53      83     399      24    1829     958    1753

  Fragments arranged by size: 

                1829    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5515
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2352    2471    4175    1239 
   Size:       1113     119    1704    2579

  Fragments arranged by size: 

                2579    1704    1113     119
 
Bpu10I CC'TnA_GC

Cuts at:   2533    2533 
   Size:       5515
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2088    2678    3640    4505    4803    2088 
   Size:        590     962     865     298    2800

  Fragments arranged by size: 

                2800     962     865     590     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4178     882 
   Size:        617    2679    2219

  Fragments arranged by size: 

                2679    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3306     493 
   Size:        711    2102    2702

  Fragments arranged by size: 

                2702    2102     711
 
BsaBI GATnn'nnATC

Cuts at:   5509    5509 
   Size:       5515
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3322    3704    4339     275 
   Size:         53      83     186     936    1789     382     635    1451

  Fragments arranged by size: 

                1789    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   3948    4346    4620    4767    5178    3948 
   Size:        398     274     147     411    4285

  Fragments arranged by size: 

                4285     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5290

  Fragments arranged by size: 

                5290     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2029    1186 
   Size:        843    4672
 
BseYI C'CCAG_C

Cuts at:   2133    4718    5214    2133 
   Size:       2585     496    2434

  Fragments arranged by size: 

                2585    2434     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2286    1163 
   Size:        573     550    4392

  Fragments arranged by size: 

                4392     573     550
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2965    3726    4754    1540 
   Size:        275     186     964     761    1028    2301

  Fragments arranged by size: 

                2301    1028     964     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2548    3576    3661    4732    2548 
   Size:       1028      85    1071    3331

  Fragments arranged by size: 

                3331    1071    1028      85
 
BsmI GAATG_Cn'

Cuts at:   3026    3119    3026 
   Size:         93    5422
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2128    2134    2148    2548    2766    2834    3576 
   Size:        399     338       6      14     400     218      68     742

Cuts at:   3576    3661    4732    1391 
   Size:         85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     400     399     338     218      85
                  68      14       6
 
BspEI T'CCGG_A

Cuts at:   5178    5178 
   Size:       5515
 
BspHI T'CATG_A

Cuts at:   3404    5134    3404 
   Size:       1730    3785
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2870     844 
   Size:       2026    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3402    1394 
   Size:        429    1579    3507

  Fragments arranged by size: 

                3507    1579     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2761    4010    4184      65 
   Size:       1183    1513    1249     174    1396

  Fragments arranged by size: 

                1513    1396    1249    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2054    4159    4346    1700 
   Size:        110     244    2105     187    2869

  Fragments arranged by size: 

                2869    2105     244     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5515
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5515
 
BssSI C'ACGA_G

Cuts at:   3575    4587    3575 
   Size:       1012    4503
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2143     794 
   Size:       1349    4166
 
BstBI TT'CG_AA

Cuts at:   2022    4319    2022 
   Size:       2297    3218
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5515
 
BstYI r'GATC_y

Cuts at:   1972    2402    2689    2845    3212    3597    3614    5066    5152 
   Size:        430     287     156     367     385      17    1452      86

Cuts at:   5152    5164    5510    1972 
   Size:         12     346    1977

  Fragments arranged by size: 

                1977    1452     430     385     367     346     287     156
                  86      17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2307     513 
   Size:        552    1242    3721

  Fragments arranged by size: 

                3721    1242     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4776

  Fragments arranged by size: 

                4776     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2353    3031    3825    3845    2353 
   Size:        678     794      20    4023

  Fragments arranged by size: 

                4023     794     678      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5515
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564    2164    2199     529 
   Size:         35    1600      35    3845

  Fragments arranged by size: 

                3845    1600      35      35
 
DraI TTT'AAA

Cuts at:   2797    2819    2797 
   Size:         22    5493
 
DraIII CAC_nnn'GTG

Cuts at:   2143    2143 
   Size:       5515
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4522     809 
   Size:        507    3206    1802

  Fragments arranged by size: 

                3206    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2962    3851    5480 
   Size:         54    1425      50     275      18    1132     889    1629

Cuts at:   5480       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425    1132     889     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2962    1537 
   Size:        275    1150    4090

  Fragments arranged by size: 

                4090    1150     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3445    1353 
   Size:       2092    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4102    4476    4622     387 
   Size:       1064     138    2513     374     146    1280

  Fragments arranged by size: 

                2513    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2352    2471    2691    3578    4175    4962    5230 
   Size:        393     720     119     220     887     597     787     268

Cuts at:   5230    1239 
   Size:       1524

  Fragments arranged by size: 

                1524     887     787     720     597     393     268     220
                 119
 
EcoRI G'AATT_C

Cuts at:   2824    3202    2824 
   Size:        378    5137
 
FspI TGC'GCA

Cuts at:   4021    4021 
   Size:       5515
 
HaeII r_GCGC'y

Cuts at:   1678    4662    1678 
   Size:       2984    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2535    2567    4156    4188    4230 
   Size:         32     135      32     963      32    1589      32      42

Cuts at:   4230    4262    1373 
   Size:         32    2626

  Fragments arranged by size: 

                2626    1589     963     135      42      32      32      32
                  32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2329    2859    3105     669 
   Size:        988     281     391     530     246    3079

  Fragments arranged by size: 

                3079     988     530     391     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5515
 
HpaI GTT'AAC

Cuts at:   3105    3105 
   Size:       5515
 
KpnI G_GTAC'C

Cuts at:   2840    2840 
   Size:       5515
 
MfeI C'AATT_G

Cuts at:   3114    3114 
   Size:       5515
 
MluI A'CGCG_T

Cuts at:   5391    5391 
   Size:       5515
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2288    4629    4813    1305 
   Size:        983    2341     184    2007

  Fragments arranged by size: 

                2341    2007     983     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3693

  Fragments arranged by size: 

                3693    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2231    3473    3832    3991     518 
   Size:        648    1065    1242     359     159    2042

  Fragments arranged by size: 

                2042    1242    1065     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2164    2300    2704    2931    3608    4756    5001    1606 
   Size:        558     136     404     227     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     677     558     404     245     227     136
            
 
NaeI GCC'GGC

Cuts at:   2056    2056 
   Size:       5515
 
NcoI C'CATG_G

Cuts at:    513    1065    2307     513 
   Size:        552    1242    3721

  Fragments arranged by size: 

                3721    1242     552
 
NdeI CA'TA_TG

Cuts at:    387    2377     387 
   Size:       1990    3525
 
NgoMIV G'CCGG_C

Cuts at:   2054    2054 
   Size:       5515
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5515
 
NotI GC'GGCC_GC

Cuts at:   2962    2962 
   Size:       5515
 
NspI r_CATG'y

Cuts at:   1132    1229    2252    2716    2873    4418    1132 
   Size:         97    1023     464     157    1545    2229

  Fragments arranged by size: 

                2229    1545    1023     464     157      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5515
 
PciI A'CATG_T

Cuts at:   2712    4414    2712 
   Size:       1702    3813
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2612    1108 
   Size:       1504    4011
 
PfoI T'CCnGG_A

Cuts at:   2560    2560 
   Size:       5515
 
PmeI GTTT'AAAC

Cuts at:   2819    2819 
   Size:       5515
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3584    3616    5097    5129    3584 
   Size:         32    1481      32    3970

  Fragments arranged by size: 

                3970    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5515
 
PsiI TTA'TAA

Cuts at:   3085    3085 
   Size:       5515
 
PstI C_TGCA'G

Cuts at:    830    2867     830 
   Size:       2037    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5515
 
PvuII CAG'CTG

Cuts at:   2164    2300    2704    2164 
   Size:        136     404    4975

  Fragments arranged by size: 

                4975     404     136
 
SalI G'TCGA_C

Cuts at:   1936    2857    1936 
   Size:        921    4594
 
SbfI CC_TGCA'GG

Cuts at:   2867    2867 
   Size:       5515
 
ScaI AGT'ACT

Cuts at:   3763    5475    3763 
   Size:       1712    3803
 
SexAI A'CCwGG_T

Cuts at:   2634    2634 
   Size:       5515
 
SfcI C'TryA_G

Cuts at:    826    1045    2863    3998    4679    4870     826 
   Size:        219    1818    1135     681     191    1471

  Fragments arranged by size: 

                1818    1471    1135     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5515
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5515
 
SmaI CCC'GGG

Cuts at:   1872    2076    2842    1872 
   Size:        204     766    4545

  Fragments arranged by size: 

                4545     766     204
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2103    2693    3619    4520    4782     820 
   Size:        197     926     160     590     926     901     262    1553

  Fragments arranged by size: 

                1553     926     926     901     590     262     197     160
            
 
SnaBI TAC'GTA

Cuts at:    493    3306     493 
   Size:       2813    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5515
 
SphI G_CATG'C

Cuts at:   1132    2873    1132 
   Size:       1741    3774
 
SspI AAT'ATT

Cuts at:      5      52    3439       5 
   Size:         47    3387    2081

  Fragments arranged by size: 

                3387    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5515
 
StyI C'CwwG_G

Cuts at:    513    1065    2307    2954     513 
   Size:        552    1242     647    3074

  Fragments arranged by size: 

                3074    1242     647     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2320    3542    3559    3712    3897    4342     325 
   Size:       1255     740    1222      17     153     185     445    1498

  Fragments arranged by size: 

                1498    1255    1222     740     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2150    3761    5473 
   Size:        274      80      33      51    1443     173    1611    1712

Cuts at:   5473      96 
   Size:        138

  Fragments arranged by size: 

                1712    1611    1443     274     173     138      80      51
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2347    2379    2572    2604    5475    5507    2347 
   Size:         32     193      32    2871      32    2355

  Fragments arranged by size: 

                2871    2355     193      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5515
 
XbaI T'CTAG_A

Cuts at:   2851    2851 
   Size:       5515
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2479    2479 
   Size:       5515
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5515
 
XmaI C'CCGG_G

Cuts at:   1870    2074    2840    1870 
   Size:        204     766    4545

  Fragments arranged by size: 

                4545     766     204
 
XmnI GAAnn'nnTTC

Cuts at:   2465    2597    3644    2465 
   Size:        132    1047    4336

  Fragments arranged by size: 

                4336    1047     132
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3323     276 
   Size:         53      83     186     936    1789    2468

  Fragments arranged by size: 

                2468    1789     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI    BbvCI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  Eco57MI    EcoRI     FspI    HaeII    Hin4I   HincII  HindIII
     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI     NspI     PasI
     PciI    PflMI     PfoI     PmeI     PpiI    PshAI     PsiI     PstI
     PvuI    PvuII     SalI     SbfI     ScaI    SexAI     SfcI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BfrBI     BplI    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I  EcoICRI
    EcoNI EcoO109I    EcoRV     FalI     FseI    FspAI     KasI     NarI
     NruI     NsiI     PacI     PmlI    PpuMI   PspOMI    PspXI     PsrI
    RsrII     SacI    SacII    SanDI     SapI     SfiI     SfoI     SrfI
     SwaI
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