(Circular) (Six-base) MAPSORT of: pFN21AB6494.seq Check: 1164 from: 1 to: 5515 pFN21AB6494 5515 bp With 182 enzymes: SgfI * April 15, 2010 09:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3325 278 Size: 53 83 186 936 1789 2468 Fragments arranged by size: 2468 1789 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2858 1937 Size: 921 4594 Acc65I G'GTAC_C Cuts at: 2836 2836 Size: 5515 AclI AA'CG_TT Cuts at: 1563 3642 4015 1563 Size: 2079 373 3063 Fragments arranged by size: 3063 2079 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2691 3578 4962 5230 1632 Size: 1059 887 1384 268 1917 Fragments arranged by size: 1917 1384 1059 887 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5318 AflIII A'CryG_T Cuts at: 1203 2712 4414 5391 1203 Size: 1509 1702 977 1327 Fragments arranged by size: 1702 1509 1327 977 AgeI A'CCGG_T Cuts at: 4346 4346 Size: 5515 AhdI GACnn_n'nnGTC Cuts at: 4244 4244 Size: 5515 AleI CACnn'nnGTG Cuts at: 1166 2231 1166 Size: 1065 4450 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5515 AlwNI CAG_nnn'CTG Cuts at: 3258 4830 3258 Size: 1572 3943 ApaLI G'TGCA_C Cuts at: 3572 4728 3572 Size: 1156 4359 ApoI r'AATT_y Cuts at: 1493 2644 2824 3050 3202 5311 1493 Size: 1151 180 226 152 2109 1697 Fragments arranged by size: 2109 1697 1151 226 180 152 AseI AT'TA_AT Cuts at: 160 1032 4069 160 Size: 872 3037 1606 Fragments arranged by size: 3037 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5515 AvaI C'yCGr_G Cuts at: 1870 1943 2074 2127 2840 1870 Size: 73 131 53 713 4545 Fragments arranged by size: 4545 713 131 73 53 BamHI G'GATC_C Cuts at: 2845 3212 2845 Size: 367 5148 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2836 4291 618 Size: 325 217 627 1049 1455 1842 Fragments arranged by size: 1842 1455 1049 627 325 217 BanII G_rGCy'C Cuts at: 1391 2128 2134 2834 1391 Size: 737 6 700 4072 Fragments arranged by size: 4072 737 700 6 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4544 BbvCI CC'TCA_GC Cuts at: 2533 2533 Size: 5515 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3706 3740 1903 Size: 34 1769 34 3678 Fragments arranged by size: 3678 1769 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2731 3407 4617 2731 Size: 676 1210 3629 Fragments arranged by size: 3629 1210 676 BclI T'GATC_A Cuts at: 1548 2490 1548 Size: 942 4573 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4126 136 Size: 107 122 71 631 605 2454 1525 Fragments arranged by size: 2454 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2402 5510 2402 Size: 3108 2407 BlpI GC'TnA_GC Cuts at: 2932 2932 Size: 5515 Bme1580I G_kGCm'C Cuts at: 1790 2148 2766 3576 4732 1790 Size: 358 618 810 1156 2573 Fragments arranged by size: 2573 1156 810 618 358 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5515 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3246 4204 442 Size: 416 53 83 399 24 1829 958 1753 Fragments arranged by size: 1829 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5515 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2352 2471 4175 1239 Size: 1113 119 1704 2579 Fragments arranged by size: 2579 1704 1113 119 Bpu10I CC'TnA_GC Cuts at: 2533 2533 Size: 5515 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2088 2678 3640 4505 4803 2088 Size: 590 962 865 298 2800 Fragments arranged by size: 2800 962 865 590 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4178 882 Size: 617 2679 2219 Fragments arranged by size: 2679 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3306 493 Size: 711 2102 2702 Fragments arranged by size: 2702 2102 711 BsaBI GATnn'nnATC Cuts at: 5509 5509 Size: 5515 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3322 3704 4339 275 Size: 53 83 186 936 1789 382 635 1451 Fragments arranged by size: 1789 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3948 4346 4620 4767 5178 3948 Size: 398 274 147 411 4285 Fragments arranged by size: 4285 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5290 Fragments arranged by size: 5290 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2029 1186 Size: 843 4672 BseYI C'CCAG_C Cuts at: 2133 4718 5214 2133 Size: 2585 496 2434 Fragments arranged by size: 2585 2434 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2286 1163 Size: 573 550 4392 Fragments arranged by size: 4392 573 550 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2965 3726 4754 1540 Size: 275 186 964 761 1028 2301 Fragments arranged by size: 2301 1028 964 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2548 3576 3661 4732 2548 Size: 1028 85 1071 3331 Fragments arranged by size: 3331 1071 1028 85 BsmI GAATG_Cn' Cuts at: 3026 3119 3026 Size: 93 5422 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2128 2134 2148 2548 2766 2834 3576 Size: 399 338 6 14 400 218 68 742 Cuts at: 3576 3661 4732 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 400 399 338 218 85 68 14 6 BspEI T'CCGG_A Cuts at: 5178 5178 Size: 5515 BspHI T'CATG_A Cuts at: 3404 5134 3404 Size: 1730 3785 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2870 844 Size: 2026 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3402 1394 Size: 429 1579 3507 Fragments arranged by size: 3507 1579 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2761 4010 4184 65 Size: 1183 1513 1249 174 1396 Fragments arranged by size: 1513 1396 1249 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2054 4159 4346 1700 Size: 110 244 2105 187 2869 Fragments arranged by size: 2869 2105 244 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5515 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5515 BssSI C'ACGA_G Cuts at: 3575 4587 3575 Size: 1012 4503 BstAPI GCAn_nnn'nTGC Cuts at: 794 2143 794 Size: 1349 4166 BstBI TT'CG_AA Cuts at: 2022 4319 2022 Size: 2297 3218 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5515 BstYI r'GATC_y Cuts at: 1972 2402 2689 2845 3212 3597 3614 5066 5152 Size: 430 287 156 367 385 17 1452 86 Cuts at: 5152 5164 5510 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 430 385 367 346 287 156 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2307 513 Size: 552 1242 3721 Fragments arranged by size: 3721 1242 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4776 Fragments arranged by size: 4776 683 56 BtsI GCAGTG_nn' Cuts at: 2353 3031 3825 3845 2353 Size: 678 794 20 4023 Fragments arranged by size: 4023 794 678 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5515 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2164 2199 529 Size: 35 1600 35 3845 Fragments arranged by size: 3845 1600 35 35 DraI TTT'AAA Cuts at: 2797 2819 2797 Size: 22 5493 DraIII CAC_nnn'GTG Cuts at: 2143 2143 Size: 5515 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4522 809 Size: 507 3206 1802 Fragments arranged by size: 3206 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2962 3851 5480 Size: 54 1425 50 275 18 1132 889 1629 Cuts at: 5480 8 Size: 43 Fragments arranged by size: 1629 1425 1132 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2962 1537 Size: 275 1150 4090 Fragments arranged by size: 4090 1150 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3445 1353 Size: 2092 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4102 4476 4622 387 Size: 1064 138 2513 374 146 1280 Fragments arranged by size: 2513 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2352 2471 2691 3578 4175 4962 5230 Size: 393 720 119 220 887 597 787 268 Cuts at: 5230 1239 Size: 1524 Fragments arranged by size: 1524 887 787 720 597 393 268 220 119 EcoRI G'AATT_C Cuts at: 2824 3202 2824 Size: 378 5137 FspI TGC'GCA Cuts at: 4021 4021 Size: 5515 HaeII r_GCGC'y Cuts at: 1678 4662 1678 Size: 2984 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2535 2567 4156 4188 4230 Size: 32 135 32 963 32 1589 32 42 Cuts at: 4230 4262 1373 Size: 32 2626 Fragments arranged by size: 2626 1589 963 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2329 2859 3105 669 Size: 988 281 391 530 246 3079 Fragments arranged by size: 3079 988 530 391 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5515 HpaI GTT'AAC Cuts at: 3105 3105 Size: 5515 KpnI G_GTAC'C Cuts at: 2840 2840 Size: 5515 MfeI C'AATT_G Cuts at: 3114 3114 Size: 5515 MluI A'CGCG_T Cuts at: 5391 5391 Size: 5515 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2288 4629 4813 1305 Size: 983 2341 184 2007 Fragments arranged by size: 2341 2007 983 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3693 Fragments arranged by size: 3693 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2231 3473 3832 3991 518 Size: 648 1065 1242 359 159 2042 Fragments arranged by size: 2042 1242 1065 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2164 2300 2704 2931 3608 4756 5001 1606 Size: 558 136 404 227 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 558 404 245 227 136 NaeI GCC'GGC Cuts at: 2056 2056 Size: 5515 NcoI C'CATG_G Cuts at: 513 1065 2307 513 Size: 552 1242 3721 Fragments arranged by size: 3721 1242 552 NdeI CA'TA_TG Cuts at: 387 2377 387 Size: 1990 3525 NgoMIV G'CCGG_C Cuts at: 2054 2054 Size: 5515 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5515 NotI GC'GGCC_GC Cuts at: 2962 2962 Size: 5515 NspI r_CATG'y Cuts at: 1132 1229 2252 2716 2873 4418 1132 Size: 97 1023 464 157 1545 2229 Fragments arranged by size: 2229 1545 1023 464 157 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5515 PciI A'CATG_T Cuts at: 2712 4414 2712 Size: 1702 3813 PflMI CCAn_nnn'nTGG Cuts at: 1108 2612 1108 Size: 1504 4011 PfoI T'CCnGG_A Cuts at: 2560 2560 Size: 5515 PmeI GTTT'AAAC Cuts at: 2819 2819 Size: 5515 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3584 3616 5097 5129 3584 Size: 32 1481 32 3970 Fragments arranged by size: 3970 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5515 PsiI TTA'TAA Cuts at: 3085 3085 Size: 5515 PstI C_TGCA'G Cuts at: 830 2867 830 Size: 2037 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5515 PvuII CAG'CTG Cuts at: 2164 2300 2704 2164 Size: 136 404 4975 Fragments arranged by size: 4975 404 136 SalI G'TCGA_C Cuts at: 1936 2857 1936 Size: 921 4594 SbfI CC_TGCA'GG Cuts at: 2867 2867 Size: 5515 ScaI AGT'ACT Cuts at: 3763 5475 3763 Size: 1712 3803 SexAI A'CCwGG_T Cuts at: 2634 2634 Size: 5515 SfcI C'TryA_G Cuts at: 826 1045 2863 3998 4679 4870 826 Size: 219 1818 1135 681 191 1471 Fragments arranged by size: 1818 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5515 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5515 SmaI CCC'GGG Cuts at: 1872 2076 2842 1872 Size: 204 766 4545 Fragments arranged by size: 4545 766 204 SmlI C'TyrA_G Cuts at: 820 1017 1943 2103 2693 3619 4520 4782 820 Size: 197 926 160 590 926 901 262 1553 Fragments arranged by size: 1553 926 926 901 590 262 197 160 SnaBI TAC'GTA Cuts at: 493 3306 493 Size: 2813 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5515 SphI G_CATG'C Cuts at: 1132 2873 1132 Size: 1741 3774 SspI AAT'ATT Cuts at: 5 52 3439 5 Size: 47 3387 2081 Fragments arranged by size: 3387 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5515 StyI C'CwwG_G Cuts at: 513 1065 2307 2954 513 Size: 552 1242 647 3074 Fragments arranged by size: 3074 1242 647 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2320 3542 3559 3712 3897 4342 325 Size: 1255 740 1222 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1222 740 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2150 3761 5473 Size: 274 80 33 51 1443 173 1611 1712 Cuts at: 5473 96 Size: 138 Fragments arranged by size: 1712 1611 1443 274 173 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2347 2379 2572 2604 5475 5507 2347 Size: 32 193 32 2871 32 2355 Fragments arranged by size: 2871 2355 193 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5515 XbaI T'CTAG_A Cuts at: 2851 2851 Size: 5515 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2479 2479 Size: 5515 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5515 XmaI C'CCGG_G Cuts at: 1870 2074 2840 1870 Size: 204 766 4545 Fragments arranged by size: 4545 766 204 XmnI GAAnn'nnTTC Cuts at: 2465 2597 3644 2465 Size: 132 1047 4336 Fragments arranged by size: 4336 1047 132 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3323 276 Size: 53 83 186 936 1789 2468 Fragments arranged by size: 2468 1789 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI