Restriction map for
pFN21AB6494
(Linear) (Six-base) MAPSORT of: KIBB6494.seq Check: 4500 from: 1 to: 810
KIBB6494.orf
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 686 810
Size: 686 124
AflIII A'CryG_T
Cuts at: 0 707 810
Size: 707 103
AleI CACnn'nnGTG
Cuts at: 0 226 810
Size: 226 584
ApoI r'AATT_y
Cuts at: 0 639 810
Size: 639 171
AvaI C'yCGr_G
Cuts at: 0 69 122 810
Size: 69 53 688
Fragments arranged by size:
688 69 53
BanII G_rGCy'C
Cuts at: 0 123 129 810
Size: 123 6 681
Fragments arranged by size:
681 123 6
BbvCI CC'TCA_GC
Cuts at: 0 528 810
Size: 528 282
BciVI GTATCCnnnnn_n'
Cuts at: 0 726 810
Size: 726 84
BclI T'GATC_A
Cuts at: 0 485 810
Size: 485 325
BglII A'GATC_T
Cuts at: 0 397 810
Size: 397 413
Bme1580I G_kGCm'C
Cuts at: 0 143 761 810
Size: 143 618 49
Fragments arranged by size:
618 143 49
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 347 466 810
Size: 347 119 344
Fragments arranged by size:
347 344 119
Bpu10I CC'TnA_GC
Cuts at: 0 528 810
Size: 528 282
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 83 673 810
Size: 83 590 137
Fragments arranged by size:
590 137 83
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 24 810
Size: 24 786
BseYI C'CCAG_C
Cuts at: 0 128 810
Size: 128 682
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 281 810
Size: 281 529
BsiHKAI G_wGCw'C
Cuts at: 0 543 810
Size: 543 267
Bsp1286I G_dGCh'C
Cuts at: 0 123 129 143 543 761 810
Size: 123 6 14 400 218 49
Fragments arranged by size:
400 218 123 49 14 6
BsrDI GCAATG_nn'
Cuts at: 0 756 810
Size: 756 54
BsrFI r'CCGG_y
Cuts at: 0 49 810
Size: 49 761
BstAPI GCAn_nnn'nTGC
Cuts at: 0 138 810
Size: 138 672
BstBI TT'CG_AA
Cuts at: 0 17 810
Size: 17 793
BstYI r'GATC_y
Cuts at: 0 397 684 810
Size: 397 287 126
Fragments arranged by size:
397 287 126
BtgI C'CryG_G
Cuts at: 0 302 810
Size: 302 508
BtsI GCAGTG_nn'
Cuts at: 0 348 810
Size: 348 462
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 159 194 810
Size: 159 35 616
Fragments arranged by size:
616 159 35
DraI TTT'AAA
Cuts at: 0 792 810
Size: 792 18
DraIII CAC_nnn'GTG
Cuts at: 0 138 810
Size: 138 672
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 347 466 686 810
Size: 347 119 220 124
Fragments arranged by size:
347 220 124 119
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 530 562 810
Size: 530 32 248
Fragments arranged by size:
530 248 32
HincII GTy'rAC
Cuts at: 0 324 810
Size: 324 486
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 810
Size: 283 527
MslI CAynn'nnrTG
Cuts at: 0 226 810
Size: 226 584
MspA1I CmG'CkG
Cuts at: 0 159 295 699 810
Size: 159 136 404 111
Fragments arranged by size:
404 159 136 111
NaeI GCC'GGC
Cuts at: 0 51 810
Size: 51 759
NcoI C'CATG_G
Cuts at: 0 302 810
Size: 302 508
NdeI CA'TA_TG
Cuts at: 0 372 810
Size: 372 438
NgoMIV G'CCGG_C
Cuts at: 0 49 810
Size: 49 761
NspI r_CATG'y
Cuts at: 0 247 711 810
Size: 247 464 99
Fragments arranged by size:
464 247 99
PciI A'CATG_T
Cuts at: 0 707 810
Size: 707 103
PflMI CCAn_nnn'nTGG
Cuts at: 0 607 810
Size: 607 203
PfoI T'CCnGG_A
Cuts at: 0 555 810
Size: 555 255
PvuII CAG'CTG
Cuts at: 0 159 295 699 810
Size: 159 136 404 111
Fragments arranged by size:
404 159 136 111
SexAI A'CCwGG_T
Cuts at: 0 629 810
Size: 629 181
SmaI CCC'GGG
Cuts at: 0 71 810
Size: 71 739
SmlI C'TyrA_G
Cuts at: 0 98 688 810
Size: 98 590 122
Fragments arranged by size:
590 122 98
StyI C'CwwG_G
Cuts at: 0 302 810
Size: 302 508
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 315 810
Size: 315 495
TatI w'GTAC_w
Cuts at: 0 145 810
Size: 145 665
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 342 374 567 599 810
Size: 342 32 193 32 211
Fragments arranged by size:
342 211 193 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 474 810
Size: 474 336
XmaI C'CCGG_G
Cuts at: 0 69 810
Size: 69 741
XmnI GAAnn'nnTTC
Cuts at: 0 460 592 810
Size: 460 132 218
Fragments arranged by size:
460 218 132
Enzymes that do cut:
AcuI AflIII AleI ApoI AvaI BanII BbvCI BciVI
BclI BglII Bme1580I BpmI Bpu10I BpuEI BseRI BseYI
BsgI BsiHKAI Bsp1286I BsrDI BsrFI BstAPI BstBI BstYI
BtgI BtsI CspCI DraI DraIII Eco57MI Hin4I HincII
MmeI MslI MspA1I NaeI NcoI NdeI NgoMIV NspI
PciI PflMI PfoI PvuII SexAI SmaI SmlI StyI
TaqII TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BbsI BcgI
BfrBI BglI BlpI BmgBI BmrI BmtI BplI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BspMI BsrBI BsrGI BssHII BssSI
BstEII BstXI BstZ17I Bsu36I BtgZI ClaI DrdI EaeI
EagI EarI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV
FalI FseI FspI FspAI HaeII HindIII HpaI KasI
KpnI MfeI MluI MscI NarI NheI NotI NruI
NsiI PacI PasI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
StuI SwaI Tth111I XbaI XhoI ZraI