Restriction map for
pFN21AE1029
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# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AflII AflIII Alw44I ApaLI AscI
AseI AsnI Asp700I Asp718I AspI
AvaI AviII AvrII BbeI BbrPI
BcgI BciVI BfrI BlnI BsaBI
BsaHI BsiEI BsiWI BsoBI BspDI
BspLU11I BsrBI BssHII BstBI BstEII
BstPI BstUI ClaI CpoI EagI
EclXI Eco52I EcoNI EcoO65I EcoRV
FseI FspI Hin1I HpaI KasI
KpnI KspI MamI MluI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI SfiI SfoI SfuI
SmaI SnaBI SphI Sse8387I SspI
Tth111I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccIII 3398 TCCGGA T^CCGGA
AgeI 30 ACCGGT A^CCGGT
AhdI 19 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 3287 AGCGCT AGC^GCT
ApaI 223 GGGCCC GGGCC^C
AspEI 19 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 207 GAGCTC G[AT]GC[AT]^C
BalI 615 TGGCCA TGG^CCA
BanI 3726 GGTGCC G^G[CT][GA]CC
BbvCI 727 CCTCAGC CCTCAGC(-5/-2)
BglII 3044 AGATCT A^GATCT
BsaAI 3301 TACGTG [CT]AC^GT[GA]
BseAI 3398 TCCGGA T^CCGGA
BsiHKAI 207 GAGCTC G[AT]GC[AT]^C
BspEI 3398 TCCGGA T^CCGGA
BspHI 449 TCATGA T^CATGA
BssSI 1487 CTCGTG CACGAG(-5/-1)
Bst1107I 2644 GTATAC GTA^TAC
BstAPI 90 GCACCGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 587 CCAATGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2644 GTATAC GTA^TAC
Eam1105I 19 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3287 AGCGCT AGC^GCT
HaeII 3287 AGCGCT [GA]GCGC^[CT]
MfeI 2078 CAATTG C^AATTG
MluNI 615 TGGCCA TGG^CCA
MroI 3398 TCCGGA T^CCGGA
MscI 615 TGGCCA TGG^CCA
MunI 2078 CAATTG C^AATTG
NspI 3660 GCATGT [GA]CATG^[CT]
PflMI 3689 CCAGCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 30 ACCGGT A^CCGGT
PpuMI 2225 AGGTCCT [GA]G^G[AT]CC[CT]
RcaI 449 TCATGA T^CATGA
SacI 207 GAGCTC GAGCT^C
SapI 1417 GCTCTTC GCTCTTC(1/4)
ScaI 164 AGTACT AGT^ACT
SgrAI 29 CACCGGTG C[GA]^CCGG[CT]G
SmlI 3123 CTCAAG C^T[CT][GA]AG
SpeI 1506 ACTAGT A^CTAGT
StuI 3632 AGGCCT AGG^CCT
SwaI 459 ATTTAAAT ATTT^AAAT
Van91I 3689 CCAGCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 31 enzymes) #
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BamHI 56 GGATCC G^GATCC
3395 GGATCC
BclI 1206 TGATCA T^GATCA
3536 TGATCA
BfaI 1507 CTAG C^TAG
3355 CTAG
BlpI 1492 GCTGAGC GC^T[ACGT]AGC
3160 GCTGAGC
Bpu1102I 1492 GCTGAGC GC^T[ACGT]AGC
3160 GCTGAGC
BsmBI 1090 CGTCTC CGTCTC(1/5)
2836 CGTCTC
Bsp1407I 1581 TGTACA T^GTACA
1708 TGTACA
BspMI 738 ACCTGC ACCTGC(4/8)
3054 GCAGGT
BsrFI 30 ACCGGT [GA]^CCGG[CT]
834 GCCGGT
BsrGI 1581 TGTACA T^GTACA
1708 TGTACA
CelII 1492 GCTGAGC GC^T[ACGT]AGC
3160 GCTGAGC
CfoI 376 GCGC GCG^C
3288 GCGC
Cfr10I 30 ACCGGT [GA]^CCGG[CT]
834 GCCGGT
DraII 2225 AGGTCCT [GA]G^G[ACGT]CC[CT]
3719 AGGGCCT
DraIII 91 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG
2386 CACCATGTG
DrdI 138 GACCATGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2218 GACACCAAGGTC
EaeI 615 TGGCCA [CT]^GGCC[GA]
782 CGGCCA
EcoO109I 2225 AGGTCCT [GA]G^G[ACGT]CC[CT]
3719 AGGGCCT
EcoT22I 815 ATGCAT ATGCA^T
2446 ATGCAT
FauI 226 CCCGC CCCGC(4/6)
1922 CCCGC
FbaI 1206 TGATCA T^GATCA
3536 TGATCA
HgaI 51 GCGTC GACGC(5/10)
3612 GCGTC
HhaI 376 GCGC GCG^C
3288 GCGC
HinP1I 376 GCGC G^CGC
3288 GCGC
HindIII 1915 AAGCTT A^AGCTT
3193 AAGCTT
MaeI 1507 CTAG C^TAG
3355 CTAG
NsiI 815 ATGCAT ATGCA^T
2446 ATGCAT
PstI 403 CTGCAG CTGCA^G
3022 CTGCAG
SexAI 185 ACCAGGT A^CC[AT]GGT
392 ACCAGGT
SspBI 1581 TGTACA T^GTACA
1708 TGTACA
XcmI 1946 CCAAATTTCGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3464 CCATGGAGAATGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI AocI ApoI AvaII
BanII BbsI BbvI BcnI BglI
BmyI BpmI BpuAI BsaI BsaJI
BsaWI BseRI BsgI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BsrDI
BsrI BssKI BstF5I BstNI BstYI
Bsu36I Cac8I Cfr13I DdeI DpnII
DraI DsaI EarI Eco81I EcoRI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
PleI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII