Restriction map for pFN21AE1029
 (Linear) (Six-base) MAPSORT of: KIEE1029.seq  Check: 9447  from: 1  to: 3819



KIEE1029.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:19  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     747    3045    3819 
   Size:        747    2298     774

  Fragments arranged by size: 

                2298     774     747
 
AccI GT'mk_AC

Cuts at:      0     428    2645    3615    3819 
   Size:        428    2217     970     204

  Fragments arranged by size: 

                2217     970     428     204
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     382     674    1360    1955    3819 
   Size:        382     292     686     595    1864

  Fragments arranged by size: 

                1864     686     595     382     292
 
AfeI AGC'GCT

Cuts at:      0    3289    3819 
   Size:       3289     530
 
AgeI A'CCGG_T

Cuts at:      0      30    3819 
   Size:         30    3789
 
AhdI GACnn_n'nnGTC

Cuts at:      0      24    3819 
   Size:         24    3795
 
AleI CACnn'nnGTG

Cuts at:      0     853    2564    3819 
   Size:        853    1711    1255

  Fragments arranged by size: 

                1711    1255     853
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1037    3819 
   Size:       1037    2782
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1290    1322    3819 
   Size:       1290      32    2497

  Fragments arranged by size: 

                2497    1290      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     133     360     712    3819 
   Size:        133     227     352    3107

  Fragments arranged by size: 

                3107     352     227     133
 
ApaI G_GGCC'C

Cuts at:      0     227    3819 
   Size:        227    3592
 
ApoI r'AATT_y

Cuts at:      0     158     457    1443    1528    1948    3261    3318    3513 
   Size:        158     299     986      85     420    1313      57     195

Cuts at:   3513    3819 
   Size:        306

  Fragments arranged by size: 

                1313     986     420     306     299     195     158      85
                  57
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1739    1772    3819 
   Size:       1739      33    2047

  Fragments arranged by size: 

                2047    1739      33
 
BamHI G'GATC_C

Cuts at:      0      56    3395    3819 
   Size:         56    3339     424

  Fragments arranged by size: 

                3339     424      56
 
BanI G'GyrC_C

Cuts at:      0    3726    3819 
   Size:       3726      93
 
BanII G_rGCy'C

Cuts at:      0     211     227    1666    3585    3819 
   Size:        211      16    1439    1919     234

  Fragments arranged by size: 

                1919    1439     234     211      16
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1810    2351    3466    3819 
   Size:       1810     541    1115     353

  Fragments arranged by size: 

                1810    1115     541     353
 
BbvCI CC'TCA_GC

Cuts at:      0     728    3819 
   Size:        728    3091
 
BclI T'GATC_A

Cuts at:      0    1206    3536    3819 
   Size:       1206    2330     283

  Fragments arranged by size: 

                2330    1206     283
 
BfrBI ATG'CAT

Cuts at:      0     817    2448    3819 
   Size:        817    1631    1371

  Fragments arranged by size: 

                1631    1371     817
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1254    1268    2545    3819 
   Size:       1254      14    1277    1274

  Fragments arranged by size: 

                1277    1274    1254      14
 
BglII A'GATC_T

Cuts at:      0    3044    3819 
   Size:       3044     775
 
BlpI GC'TnA_GC

Cuts at:      0    1493    3161    3819 
   Size:       1493    1668     658

  Fragments arranged by size: 

                1668    1493     658
 
Bme1580I G_kGCm'C

Cuts at:      0     227    1978    3668    3731    3819 
   Size:        227    1751    1690      63      88

  Fragments arranged by size: 

                1751    1690     227      88      63
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     690    3819 
   Size:        690    3129
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     323    1500    2506    3819 
   Size:        323    1177    1006    1313

  Fragments arranged by size: 

                1313    1177    1006     323
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1006    1034    1055    1292    1650    1990    3157    3514 
   Size:       1006      28      21     237     358     340    1167     357

Cuts at:   3514    3736    3819 
   Size:        222      83

  Fragments arranged by size: 

                1167    1006     358     357     340     237     222      83
                  28      21
 
Bpu10I CC'TnA_GC

Cuts at:      0     728    3338    3819 
   Size:        728    2610     481

  Fragments arranged by size: 

                2610     728     481
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    3108    3819 
   Size:       3108     711
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     130    1864    3679    3748    3819 
   Size:        130    1734    1815      69      71

  Fragments arranged by size: 

                1815    1734     130      71      69
 
BsaAI yAC'GTr

Cuts at:      0    3303    3819 
   Size:       3303     516
 
BsaWI w'CCGG_w

Cuts at:      0      30    3097    3398    3819 
   Size:         30    3067     301     421

  Fragments arranged by size: 

                3067     421     301      30
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     275     305    1205    1235    1290    1320    3130    3160 
   Size:        275      30     900      30      55      30    1810      30

Cuts at:   3160    3819 
   Size:        659

  Fragments arranged by size: 

                1810     900     659     275      55      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     716    1712    2311    3271    3819 
   Size:        716     996     599     960     548

  Fragments arranged by size: 

                 996     960     716     599     548
 
BseYI C'CCAG_C

Cuts at:      0      44     178     506     683     705     716    1258    2790 
   Size:         44     134     328     177      22      11     542    1532

Cuts at:   2790    2877    3819 
   Size:         87     942

  Fragments arranged by size: 

                1532     942     542     328     177     134      87      44
                  22      11
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     292     311     355    1448    2677    3073    3819 
   Size:        292      19      44    1093    1229     396     746

  Fragments arranged by size: 

                1229    1093     746     396     292      44      19
 
BsiHKAI G_wGCw'C

Cuts at:      0     211    3819 
   Size:        211    3608
 
BsmI GAATG_Cn'

Cuts at:      0     979    2307    2408    3819 
   Size:        979    1328     101    1411

  Fragments arranged by size: 

                1411    1328     979     101
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1096    2842    3819 
   Size:       1096    1746     977

  Fragments arranged by size: 

                1746    1096     977
 
Bsp1286I G_dGCh'C

Cuts at:      0     211     227    1666    1978    3585    3668    3731    3819 
   Size:        211      16    1439     312    1607      83      63      88

  Fragments arranged by size: 

                1607    1439     312     211      88      83      63      16
            
 
BspEI T'CCGG_A

Cuts at:      0    3398    3819 
   Size:       3398     421
 
BspHI T'CATG_A

Cuts at:      0     449    3819 
   Size:        449    3370
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     747    3045    3819 
   Size:        747    2298     774

  Fragments arranged by size: 

                2298     774     747
 
BsrDI GCAATG_nn'

Cuts at:      0    1622    2066    2975    3819 
   Size:       1622     444     909     844

  Fragments arranged by size: 

                1622     909     844     444
 
BsrFI r'CCGG_y

Cuts at:      0      30     834    3819 
   Size:         30     804    2985

  Fragments arranged by size: 

                2985     804      30
 
BsrGI T'GTAC_A

Cuts at:      0    1581    1708    3819 
   Size:       1581     127    2111

  Fragments arranged by size: 

                2111    1581     127
 
BssSI C'ACGA_G

Cuts at:      0    1487    3819 
   Size:       1487    2332
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      96    3819 
   Size:         96    3723
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     594    3819 
   Size:        594    3225
 
BstYI r'GATC_y

Cuts at:      0      56    1277    3044    3395    3819 
   Size:         56    1221    1767     351     424

  Fragments arranged by size: 

                1767    1221     424     351      56
 
BstZ17I GTA'TAC

Cuts at:      0    2646    3819 
   Size:       2646    1173
 
Bsu36I CC'TnA_GG

Cuts at:      0     422    2230    2710    3819 
   Size:        422    1808     480    1109

  Fragments arranged by size: 

                1808    1109     480     422
 
BtgI C'CryG_G

Cuts at:      0     279     639    2987    3464    3819 
   Size:        279     360    2348     477     355

  Fragments arranged by size: 

                2348     477     360     355     279
 
BtsI GCAGTG_nn'

Cuts at:      0     134    3819 
   Size:        134    3685
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1356    1391    3819 
   Size:       1356      35    2428

  Fragments arranged by size: 

                2428    1356      35
 
DraI TTT'AAA

Cuts at:      0     462    1098    1554    1742    2208    3819 
   Size:        462     636     456     188     466    1611

  Fragments arranged by size: 

                1611     636     466     462     456     188
 
DraIII CAC_nnn'GTG

Cuts at:      0      96    2391    3819 
   Size:         96    2295    1428

  Fragments arranged by size: 

                2295    1428      96
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     144    2224    3819 
   Size:        144    2080    1595

  Fragments arranged by size: 

                2080    1595     144
 
EaeI y'GGCC_r

Cuts at:      0     615     782    3819 
   Size:        615     167    3037

  Fragments arranged by size: 

                3037     615     167
 
EarI CTCTTCn'nnn_

Cuts at:      0    1424    2389    2501    2513    2659    3248    3819 
   Size:       1424     965     112      12     146     589     571

  Fragments arranged by size: 

                1424     965     589     571     146     112      12
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     821    3408    3819 
   Size:        821    2587     411

  Fragments arranged by size: 

                2587     821     411
 
EcoICRI GAG'CTC

Cuts at:      0     209    3819 
   Size:        209    3610
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     382     674    1006    1034    1055    1292    1360    1650 
   Size:        382     292     332      28      21     237      68     290

Cuts at:   1650    1955    1990    3157    3514    3736    3819 
   Size:        305      35    1167     357     222      83

  Fragments arranged by size: 

                1167     382     357     332     305     292     290     237
                 222      83      68      35      28      21
 
EcoO109I rG'GnC_Cy

Cuts at:      0    2226    3720    3819 
   Size:       2226    1494      99

  Fragments arranged by size: 

                2226    1494      99
 
EcoRI G'AATT_C

Cuts at:      0     158    3261    3318    3513    3819 
   Size:        158    3103      57     195     306

  Fragments arranged by size: 

                3103     306     195     158      57
 
HaeII r_GCGC'y

Cuts at:      0    3291    3819 
   Size:       3291     528
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      10      42     364     396    1102    1134    1191    1205 
   Size:         10      32     322      32     706      32      57      14

Cuts at:   1205    1223    1237    2043    2075    3277    3309    3316    3348 
   Size:         18      14     806      32    1202      32       7      32

Cuts at:   3348    3819 
   Size:        471

  Fragments arranged by size: 

                1202     806     706     471     322      57      32      32
                  32      32      32      32      18      14      14      10
                   7
 
HincII GTy'rAC

Cuts at:      0    2664    3143    3409    3819 
   Size:       2664     479     266     410

  Fragments arranged by size: 

                2664     479     410     266
 
HindIII A'AGCT_T

Cuts at:      0    1915    3193    3819 
   Size:       1915    1278     626

  Fragments arranged by size: 

                1915    1278     626
 
MfeI C'AATT_G

Cuts at:      0    2078    3819 
   Size:       2078    1741
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      34    3819 
   Size:         34    3785
 
MscI TGG'CCA

Cuts at:      0     617    3819 
   Size:        617    3202
 
MslI CAynn'nnrTG

Cuts at:      0     853    1376    1627    2564    3599    3819 
   Size:        853     523     251     937    1035     220

  Fragments arranged by size: 

                1035     937     853     523     251     220
 
MspA1I CmG'CkG

Cuts at:      0     268     325    2032    3021    3108    3153    3228    3819 
   Size:        268      57    1707     989      87      45      75     591

  Fragments arranged by size: 

                1707     989     591     268      87      75      57      45
            
 
NcoI C'CATG_G

Cuts at:      0     639    2987    3464    3819 
   Size:        639    2348     477     355

  Fragments arranged by size: 

                2348     639     477     355
 
NsiI A_TGCA'T

Cuts at:      0     819    2450    3819 
   Size:        819    1631    1369

  Fragments arranged by size: 

                1631    1369     819
 
NspI r_CATG'y

Cuts at:      0    3664    3819 
   Size:       3664     155
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    3695    3819 
   Size:       3695     124
 
PfoI T'CCnGG_A

Cuts at:      0     983    2885    3134    3819 
   Size:        983    1902     249     685

  Fragments arranged by size: 

                1902     983     685     249
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1290    1322    3819 
   Size:       1290      32    2497

  Fragments arranged by size: 

                2497    1290      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    2226    3819 
   Size:       2226    1593
 
PspOMI G'GGCC_C

Cuts at:      0     223    3819 
   Size:        223    3596
 
PstI C_TGCA'G

Cuts at:      0     407    3026    3819 
   Size:        407    2619     793

  Fragments arranged by size: 

                2619     793     407
 
PvuII CAG'CTG

Cuts at:      0     325    2032    3021    3108    3228    3819 
   Size:        325    1707     989      87     120     591

  Fragments arranged by size: 

                1707     989     591     325     120      87
 
SacI G_AGCT'C

Cuts at:      0     211    3819 
   Size:        211    3608
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1424    3819 
   Size:       1424    2395
 
ScaI AGT'ACT

Cuts at:      0     166    3819 
   Size:        166    3653
 
SexAI A'CCwGG_T

Cuts at:      0     185     392    3819 
   Size:        185     207    3427

  Fragments arranged by size: 

                3427     207     185
 
SfcI C'TryA_G

Cuts at:      0     230     403    1292    3022    3819 
   Size:        230     173     889    1730     797

  Fragments arranged by size: 

                1730     889     797     230     173
 
SgrAI Cr'CCGG_yG

Cuts at:      0      30    3819 
   Size:         30    3789
 
SmlI C'TyrA_G

Cuts at:      0    3123    3819 
   Size:       3123     696
 
SpeI A'CTAG_T

Cuts at:      0    1506    3819 
   Size:       1506    2313
 
StuI AGG'CCT

Cuts at:      0    3634    3819 
   Size:       3634     185
 
StyI C'CwwG_G

Cuts at:      0     639    1025    1968    2222    2987    3213    3464    3653 
   Size:        639     386     943     254     765     226     251     189

Cuts at:   3653    3819 
   Size:        166

  Fragments arranged by size: 

                 943     765     639     386     254     251     226     189
                 166
 
SwaI ATTT'AAAT

Cuts at:      0     462    3819 
   Size:        462    3357
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     192     275     652    3694    3819 
   Size:        192      83     377    3042     125

  Fragments arranged by size: 

                3042     377     192     125      83
 
TatI w'GTAC_w

Cuts at:      0     164    1581    1708    3819 
   Size:        164    1417     127    2111

  Fragments arranged by size: 

                2111    1417     164     127
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     275     307    3819 
   Size:        275      32    3512

  Fragments arranged by size: 

                3512     275      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1953    3471    3819 
   Size:       1953    1518     348

  Fragments arranged by size: 

                1953    1518     348
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AfeI     AgeI     AhdI     AleI     AlfI
     AloI    AlwNI     ApaI     ApoI     BaeI    BamHI     BanI    BanII
     BbsI    BbvCI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaWI
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI
    BstXI    BstYI  BstZ17I   Bsu36I     BtgI     BtsI    CspCI     DraI
   DraIII     DrdI     EaeI     EarI     EciI  EcoICRI  Eco57MI EcoO109I
    EcoRI    HaeII    Hin4I   HincII  HindIII     MfeI     MmeI     MscI
     MslI   MspA1I     NcoI     NsiI     NspI    PflMI     PfoI     PpiI
    PpuMI   PspOMI     PstI    PvuII     SacI     SapI     ScaI    SexAI
     SfcI    SgrAI     SmlI     SpeI     StuI     StyI     SwaI    TaqII
     TatI     TstI     XcmI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI    AflII   AflIII    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BbeI     BcgI    BciVI    BmgBI     BmtI
    BsaBI    BsaHI    BsiEI    BsiWI    BsrBI   BssHII    BstBI   BstEII
    BtgZI     ClaI     EagI    EcoNI    EcoRV     FalI     FseI     FspI
    FspAI     HpaI     KasI     KpnI     MluI     NaeI     NarI     NdeI
   NgoMIV     NheI     NotI     NruI     PacI     PasI     PciI     PmeI
     PmlI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII    SacII
     SalI    SanDI     SbfI     SfiI     SfoI     SgfI     SmaI    SnaBI
     SphI     SrfI     SspI  Tth111I     XbaI     XhoI     XmaI     XmnI
     ZraI
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