(Circular) (Six-base) MAPSORT of: pFN21AB4741.seq Check: 9352 from: 1 to: 8140 pFN21AB4741 8140 bp With 182 enzymes: SgfI * October 20, 2009 10:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5193 5950 278 Size: 53 83 186 936 3657 757 2468 Fragments arranged by size: 3657 2468 936 757 186 83 53 AccI GT'mk_AC Cuts at: 1937 5483 1937 Size: 3546 4594 Acc65I G'GTAC_C Cuts at: 5461 5461 Size: 8140 AclI AA'CG_TT Cuts at: 1563 3070 6267 6640 1563 Size: 1507 3197 373 3063 Fragments arranged by size: 3197 3063 1507 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4027 4039 4629 4658 5004 5358 5376 6203 Size: 2395 12 590 29 346 354 18 827 Cuts at: 6203 7587 7855 1632 Size: 1384 268 1917 Fragments arranged by size: 2395 1917 1384 827 590 354 346 268 29 18 12 AfeI AGC'GCT Cuts at: 2155 2155 Size: 8140 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7943 AflIII A'CryG_T Cuts at: 1203 2201 7039 8016 1203 Size: 998 4838 977 1327 Fragments arranged by size: 4838 1327 998 977 AgeI A'CCGG_T Cuts at: 6971 6971 Size: 8140 AhdI GACnn_n'nnGTC Cuts at: 6869 6869 Size: 8140 AleI CACnn'nnGTG Cuts at: 1166 4080 1166 Size: 2914 5226 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4098 4929 1184 Size: 2914 831 4395 Fragments arranged by size: 4395 2914 831 AlwNI CAG_nnn'CTG Cuts at: 5883 7455 5883 Size: 1572 6568 ApaLI G'TGCA_C Cuts at: 4073 6197 7353 4073 Size: 2124 1156 4860 Fragments arranged by size: 4860 2124 1156 ApoI r'AATT_y Cuts at: 1493 2863 3488 4282 4361 4441 4975 5261 5449 Size: 1370 625 794 79 80 534 286 188 Cuts at: 5449 5675 5827 7936 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1370 794 625 534 286 226 188 152 80 79 AseI AT'TA_AT Cuts at: 160 1032 2405 4931 6694 160 Size: 872 1373 2526 1763 1606 Fragments arranged by size: 2526 1763 1606 1373 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8140 AvaI C'yCGr_G Cuts at: 1870 1943 3840 5465 1870 Size: 73 1897 1625 4545 Fragments arranged by size: 4545 1897 1625 73 BamHI G'GATC_C Cuts at: 4000 4327 5470 5837 4000 Size: 327 1143 367 6303 Fragments arranged by size: 6303 1143 367 327 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3991 5318 5461 6916 618 Size: 325 217 627 2204 1327 143 1455 1842 Fragments arranged by size: 2204 1842 1455 1327 627 325 217 143 BanII G_rGCy'C Cuts at: 1391 2144 5459 1391 Size: 753 3315 4072 Fragments arranged by size: 4072 3315 753 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3478 928 Size: 971 1579 5590 Fragments arranged by size: 5590 1579 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4179 4213 6331 6365 1903 Size: 34 2242 34 2118 34 3678 Fragments arranged by size: 3678 2242 2118 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6032 7242 6032 Size: 1210 6930 BclI T'GATC_A Cuts at: 1548 2844 1548 Size: 1296 6844 BfrBI ATG'CAT Cuts at: 3025 4580 3025 Size: 1555 6585 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6751 136 Size: 107 122 71 631 605 5079 1525 Fragments arranged by size: 5079 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3148 8135 3148 Size: 4987 3153 BlpI GC'TnA_GC Cuts at: 5557 5557 Size: 8140 Bme1580I G_kGCm'C Cuts at: 1790 3839 4077 5323 6201 7357 1790 Size: 2049 238 1246 878 1156 2573 Fragments arranged by size: 2573 2049 1246 1156 878 238 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8140 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4216 4329 4756 Size: 416 53 83 399 24 2799 113 427 Cuts at: 4756 5871 6829 442 Size: 1115 958 1753 Fragments arranged by size: 2799 1753 1115 958 427 416 399 113 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8140 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5028 5028 Size: 8140 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3228 3751 4411 4915 6800 1239 Size: 1989 523 660 504 1885 2579 Fragments arranged by size: 2579 1989 1885 660 523 504 Bpu10I CC'TnA_GC Cuts at: 4184 4626 4184 Size: 442 7698 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 6265 7130 7428 6265 Size: 865 298 6977 Fragments arranged by size: 6977 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4637 4716 6803 882 Size: 617 3138 79 2087 2219 Fragments arranged by size: 3138 2219 2087 617 79 BsaAI yAC'GTr Cuts at: 493 1204 5931 493 Size: 711 4727 2702 Fragments arranged by size: 4727 2702 711 BsaBI GATnn'nnATC Cuts at: 8134 8134 Size: 8140 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5190 5947 6329 6964 Size: 53 83 186 936 3657 757 382 635 Cuts at: 6964 275 Size: 1451 Fragments arranged by size: 3657 1451 936 757 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6573 6971 7245 7392 7803 6573 Size: 398 274 147 411 6910 Fragments arranged by size: 6910 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3843 3873 1178 Size: 30 165 30 2440 30 5445 Fragments arranged by size: 5445 2440 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2961 4004 1186 Size: 1775 1043 5322 Fragments arranged by size: 5322 1775 1043 BseYI C'CCAG_C Cuts at: 7343 7839 7343 Size: 496 7644 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2718 3678 3828 1163 Size: 573 982 960 150 5475 Fragments arranged by size: 5475 982 960 573 150 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5590 6351 7379 1540 Size: 275 186 3589 761 1028 2301 Fragments arranged by size: 3589 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2971 4077 4741 6201 6286 7357 2971 Size: 1106 664 1460 85 1071 3754 Fragments arranged by size: 3754 1460 1106 1071 664 85 BsmI GAATG_Cn' Cuts at: 4042 5651 5744 4042 Size: 1609 93 6438 Fragments arranged by size: 6438 1609 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4350 4350 Size: 8140 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2144 2971 3839 4077 4741 5323 5459 Size: 399 354 827 868 238 664 582 136 Cuts at: 5459 6201 6286 7357 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 868 827 742 664 582 399 354 238 136 85 BspEI T'CCGG_A Cuts at: 7803 7803 Size: 8140 BspHI T'CATG_A Cuts at: 6029 7759 6029 Size: 1730 6410 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5495 844 Size: 4651 3489 BsrBI CCG'CTC Cuts at: 1394 1823 6027 1394 Size: 429 4204 3507 Fragments arranged by size: 4204 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2910 2949 3687 5193 6635 6809 65 Size: 1183 1662 39 738 1506 1442 174 1396 Fragments arranged by size: 1662 1506 1442 1396 1183 738 174 39 BsrFI r'CCGG_y Cuts at: 1700 1810 6784 6971 1700 Size: 110 4974 187 2869 Fragments arranged by size: 4974 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2453 96 Size: 2357 5783 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8140 BssSI C'ACGA_G Cuts at: 5371 6200 7212 5371 Size: 829 1012 6299 Fragments arranged by size: 6299 1012 829 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8140 BstBI TT'CG_AA Cuts at: 6944 6944 Size: 8140 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8140 BstYI r'GATC_y Cuts at: 1972 3148 3344 4000 4327 5036 5470 5837 6222 Size: 1176 196 656 327 709 434 367 385 Cuts at: 6222 6239 7691 7777 7789 8135 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1176 709 656 434 385 367 346 327 196 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3996 3996 Size: 8140 BtgI C'CryG_G Cuts at: 513 1065 3503 3985 513 Size: 552 2438 482 4668 Fragments arranged by size: 4668 2438 552 482 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7401 Fragments arranged by size: 7401 683 56 BtsI GCAGTG_nn' Cuts at: 4075 4087 5023 5656 6450 6470 4075 Size: 12 936 633 794 20 5745 Fragments arranged by size: 5745 936 794 633 20 12 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8140 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3745 3780 529 Size: 35 3181 35 4889 Fragments arranged by size: 4889 3181 35 35 DraI TTT'AAA Cuts at: 2490 2868 5427 5444 2490 Size: 378 2559 17 5186 Fragments arranged by size: 5186 2559 378 17 DraIII CAC_nnn'GTG Cuts at: 3991 4337 5318 3991 Size: 346 981 6813 Fragments arranged by size: 6813 981 346 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7147 809 Size: 507 5831 1802 Fragments arranged by size: 5831 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3818 3977 5587 Size: 54 1425 50 275 18 1988 159 1610 Cuts at: 5587 6476 8105 8 Size: 889 1629 43 Fragments arranged by size: 1988 1629 1610 1425 889 275 159 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5587 1537 Size: 275 3775 4090 Fragments arranged by size: 4090 3775 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3825 3940 5091 6070 1353 Size: 2472 115 1151 979 3423 Fragments arranged by size: 3423 2472 1151 979 115 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6727 7101 7247 387 Size: 1064 138 5138 374 146 1280 Fragments arranged by size: 5138 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3228 3751 4027 4039 4411 4629 4658 Size: 393 1596 523 276 12 372 218 29 Cuts at: 4658 4915 5004 5358 5376 6203 6800 7587 7855 Size: 257 89 354 18 827 597 787 268 Cuts at: 7855 1239 Size: 1524 Fragments arranged by size: 1596 1524 827 787 597 523 393 372 354 276 268 257 218 89 29 18 12 EcoO109I rG'GnC_Cy Cuts at: 3701 3701 Size: 8140 EcoRI G'AATT_C Cuts at: 4361 4441 4975 5449 5827 4361 Size: 80 534 474 378 6674 Fragments arranged by size: 6674 534 474 378 80 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3491 5439 3491 Size: 1948 6192 FspI TGC'GCA Cuts at: 6646 6646 Size: 8140 HaeII r_GCGC'y Cuts at: 1678 2157 7287 1678 Size: 479 5130 2531 Fragments arranged by size: 5130 2531 479 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3617 3649 4517 4549 4609 Size: 32 135 32 2045 32 868 32 60 Cuts at: 4609 4641 6781 6813 6855 6887 1373 Size: 32 2140 32 42 32 2626 Fragments arranged by size: 2626 2140 2045 868 135 60 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3574 4786 5484 5730 669 Size: 988 281 1636 1212 698 246 3079 Fragments arranged by size: 3079 1636 1212 988 698 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8140 HpaI GTT'AAC Cuts at: 5730 5730 Size: 8140 KpnI G_GTAC'C Cuts at: 5465 5465 Size: 8140 MfeI C'AATT_G Cuts at: 5739 5739 Size: 8140 MluI A'CGCG_T Cuts at: 8016 8016 Size: 8140 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3417 7254 7438 1305 Size: 2112 3837 184 2007 Fragments arranged by size: 3837 2112 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3979 10 Size: 54 1425 343 2147 4171 Fragments arranged by size: 4171 2147 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2228 4070 4080 6098 6457 6616 Size: 648 843 219 1842 10 2018 359 159 Cuts at: 6616 518 Size: 2042 Fragments arranged by size: 2042 2018 1842 843 648 359 219 159 10 MspA1I CmG'CkG Cuts at: 1606 3505 3975 5556 6233 7381 7626 1606 Size: 1899 470 1581 677 1148 245 2120 Fragments arranged by size: 2120 1899 1581 1148 677 470 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7588 NdeI CA'TA_TG Cuts at: 387 4459 4768 387 Size: 4072 309 3759 Fragments arranged by size: 4072 3759 309 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8140 NotI GC'GGCC_GC Cuts at: 5587 5587 Size: 8140 NsiI A_TGCA'T Cuts at: 3027 4582 3027 Size: 1555 6585 NspI r_CATG'y Cuts at: 1132 1229 2205 2903 3029 5498 7043 1132 Size: 97 976 698 126 2469 1545 2229 Fragments arranged by size: 2469 2229 1545 976 698 126 97 PasI CC'CwG_GG Cuts at: 1348 3045 3705 1348 Size: 1697 660 5783 Fragments arranged by size: 5783 1697 660 PciI A'CATG_T Cuts at: 2201 7039 2201 Size: 4838 3302 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 8140 PfoI T'CCnGG_A Cuts at: 2695 3205 2695 Size: 510 7630 PmeI GTTT'AAAC Cuts at: 5444 5444 Size: 8140 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6209 6241 7722 7754 6209 Size: 32 1481 32 6595 Fragments arranged by size: 6595 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3701 3701 Size: 8140 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8140 PsiI TTA'TAA Cuts at: 3243 3447 3594 4589 5710 3243 Size: 204 147 995 1121 5673 Fragments arranged by size: 5673 1121 995 204 147 PstI C_TGCA'G Cuts at: 830 4082 4918 5030 5492 830 Size: 3252 836 112 462 3478 Fragments arranged by size: 3478 3252 836 462 112 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8140 PvuII CAG'CTG Cuts at: 3975 3975 Size: 8140 SacII CC_GC'GG Cuts at: 3506 3506 Size: 8140 SalI G'TCGA_C Cuts at: 1936 5482 1936 Size: 3546 4594 SbfI CC_TGCA'GG Cuts at: 5492 5492 Size: 8140 ScaI AGT'ACT Cuts at: 4745 6388 8100 4745 Size: 1643 1712 4785 Fragments arranged by size: 4785 1712 1643 SfcI C'TryA_G Cuts at: 826 1045 2539 4078 4914 5026 5488 6623 7304 Size: 219 1494 1539 836 112 462 1135 681 Cuts at: 7304 7495 826 Size: 191 1471 Fragments arranged by size: 1539 1494 1471 1135 836 681 462 219 191 112 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8140 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8140 SmaI CCC'GGG Cuts at: 1872 5467 1872 Size: 3595 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 6244 7145 7407 820 Size: 197 926 4301 901 262 1553 Fragments arranged by size: 4301 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5931 493 Size: 5438 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8140 SphI G_CATG'C Cuts at: 1132 5498 1132 Size: 4366 3774 SspI AAT'ATT Cuts at: 5 52 2409 2503 2533 3158 5055 6064 5 Size: 47 2357 94 30 625 1897 1009 2081 Fragments arranged by size: 2357 2081 1897 1009 625 94 47 30 StuI AGG'CCT Cuts at: 1517 3040 3182 4137 1517 Size: 1523 142 955 5520 Fragments arranged by size: 5520 1523 955 142 StyI C'CwwG_G Cuts at: 513 1065 4923 5579 513 Size: 552 3858 656 3074 Fragments arranged by size: 3858 3074 656 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4348 6167 6184 6337 6522 6967 325 Size: 1255 2768 1819 17 153 185 445 1498 Fragments arranged by size: 2768 1819 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2384 2453 4224 Size: 274 80 33 51 1443 407 69 1771 Cuts at: 4224 4743 5006 6386 8098 96 Size: 519 263 1380 1712 138 Fragments arranged by size: 1771 1712 1443 1380 519 407 274 263 138 80 69 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3790 3822 3936 3968 8100 8132 3790 Size: 32 114 32 4132 32 3798 Fragments arranged by size: 4132 3798 114 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8140 XbaI T'CTAG_A Cuts at: 5476 5476 Size: 8140 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2982 2982 Size: 8140 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8140 XmaI C'CCGG_G Cuts at: 1870 5465 1870 Size: 3595 4545 XmnI GAAnn'nnTTC Cuts at: 2436 4040 6269 2436 Size: 1604 2229 4307 Fragments arranged by size: 4307 2229 1604 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5191 5948 276 Size: 53 83 186 936 3657 757 2468 Fragments arranged by size: 3657 2468 936 757 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BsiWI BstXI BstZ17I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI