Restriction map for
pFN21AB4741
(Linear) (Six-base) MAPSORT of: KIBB4741.seq Check: 2326 from: 1 to: 3435
KIBB4741.orf
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AatII G_ACGT'C
Cuts at: 0 3188 3435
Size: 3188 247
AclI AA'CG_TT
Cuts at: 0 1065 3435
Size: 1065 2370
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2022 2034 2624 2653 2999 3353 3371 3435
Size: 2022 12 590 29 346 354 18 64
Fragments arranged by size:
2022 590 354 346 64 29 18 12
AfeI AGC'GCT
Cuts at: 0 150 3435
Size: 150 3285
AflIII A'CryG_T
Cuts at: 0 196 3435
Size: 196 3239
AleI CACnn'nnGTG
Cuts at: 0 2075 3435
Size: 2075 1360
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2093 2924 3435
Size: 2093 831 511
Fragments arranged by size:
2093 831 511
ApaLI G'TGCA_C
Cuts at: 0 2068 3435
Size: 2068 1367
ApoI r'AATT_y
Cuts at: 0 858 1483 2277 2356 2436 2970 3256 3435
Size: 858 625 794 79 80 534 286 179
Fragments arranged by size:
858 794 625 534 286 179 80 79
AseI AT'TA_AT
Cuts at: 0 400 2926 3435
Size: 400 2526 509
Fragments arranged by size:
2526 509 400
AvaI C'yCGr_G
Cuts at: 0 1835 3435
Size: 1835 1600
BamHI G'GATC_C
Cuts at: 0 1995 2322 3435
Size: 1995 327 1113
Fragments arranged by size:
1995 1113 327
BanI G'GyrC_C
Cuts at: 0 1986 3313 3435
Size: 1986 1327 122
Fragments arranged by size:
1986 1327 122
BanII G_rGCy'C
Cuts at: 0 139 3435
Size: 139 3296
BbsI GAAGACnn'nnnn_
Cuts at: 0 1473 3435
Size: 1473 1962
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2174 2208 3435
Size: 2174 34 1227
Fragments arranged by size:
2174 1227 34
BclI T'GATC_A
Cuts at: 0 839 3435
Size: 839 2596
BfrBI ATG'CAT
Cuts at: 0 1020 2575 3435
Size: 1020 1555 860
Fragments arranged by size:
1555 1020 860
BglII A'GATC_T
Cuts at: 0 1143 3435
Size: 1143 2292
Bme1580I G_kGCm'C
Cuts at: 0 1834 2072 3318 3435
Size: 1834 238 1246 117
Fragments arranged by size:
1834 1246 238 117
BmrI ACTGGGnnnn_n'
Cuts at: 0 2211 2324 2751 3435
Size: 2211 113 427 684
Fragments arranged by size:
2211 684 427 113
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3023 3435
Size: 3023 412
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1223 1746 2406 2910 3435
Size: 1223 523 660 504 525
Fragments arranged by size:
1223 660 525 523 504
Bpu10I CC'TnA_GC
Cuts at: 0 2179 2621 3435
Size: 2179 442 814
Fragments arranged by size:
2179 814 442
BsaI GGTCTCn'nnnn_
Cuts at: 0 2632 2711 3435
Size: 2632 79 724
Fragments arranged by size:
2632 724 79
BsaHI Gr'CG_yC
Cuts at: 0 3185 3435
Size: 3185 250
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1838 1868 3435
Size: 1838 30 1567
Fragments arranged by size:
1838 1567 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 956 1999 3435
Size: 956 1043 1436
Fragments arranged by size:
1436 1043 956
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 713 1673 1823 3435
Size: 713 960 150 1612
Fragments arranged by size:
1612 960 713 150
BsiHKAI G_wGCw'C
Cuts at: 0 966 2072 2736 3435
Size: 966 1106 664 699
Fragments arranged by size:
1106 966 699 664
BsmI GAATG_Cn'
Cuts at: 0 2037 3435
Size: 2037 1398
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2345 3435
Size: 2345 1090
Bsp1286I G_dGCh'C
Cuts at: 0 139 966 1834 2072 2736 3318 3435
Size: 139 827 868 238 664 582 117
Fragments arranged by size:
868 827 664 582 238 139 117
BsrDI GCAATG_nn'
Cuts at: 0 905 944 1682 3188 3435
Size: 905 39 738 1506 247
Fragments arranged by size:
1506 905 738 247 39
BsrGI T'GTAC_A
Cuts at: 0 448 3435
Size: 448 2987
BssSI C'ACGA_G
Cuts at: 0 3366 3435
Size: 3366 69
BstYI r'GATC_y
Cuts at: 0 1143 1339 1995 2322 3031 3435
Size: 1143 196 656 327 709 404
Fragments arranged by size:
1143 709 656 404 327 196
Bsu36I CC'TnA_GG
Cuts at: 0 1991 3435
Size: 1991 1444
BtgI C'CryG_G
Cuts at: 0 1498 1980 3435
Size: 1498 482 1455
Fragments arranged by size:
1498 1455 482
BtsI GCAGTG_nn'
Cuts at: 0 2070 2082 3018 3435
Size: 2070 12 936 417
Fragments arranged by size:
2070 936 417 12
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1740 1775 3435
Size: 1740 35 1660
Fragments arranged by size:
1740 1660 35
DraI TTT'AAA
Cuts at: 0 485 863 3422 3435
Size: 485 378 2559 13
Fragments arranged by size:
2559 485 378 13
DraIII CAC_nnn'GTG
Cuts at: 0 1986 2332 3313 3435
Size: 1986 346 981 122
Fragments arranged by size:
1986 981 346 122
EaeI y'GGCC_r
Cuts at: 0 1813 1972 3435
Size: 1813 159 1463
Fragments arranged by size:
1813 1463 159
EarI CTCTTCn'nnn_
Cuts at: 0 1820 1935 3086 3435
Size: 1820 115 1151 349
Fragments arranged by size:
1820 1151 349 115
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1223 1746 2022 2034 2406 2624 2653 2910
Size: 1223 523 276 12 372 218 29 257
Cuts at: 2910 2999 3353 3371 3435
Size: 89 354 18 64
Fragments arranged by size:
1223 523 372 354 276 257 218 89
64 29 18 12
EcoO109I rG'GnC_Cy
Cuts at: 0 1696 3435
Size: 1696 1739
EcoRI G'AATT_C
Cuts at: 0 2356 2436 2970 3435
Size: 2356 80 534 465
Fragments arranged by size:
2356 534 465 80
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1486 3434 3435
Size: 1486 1948 1
Fragments arranged by size:
1948 1486 1
HaeII r_GCGC'y
Cuts at: 0 152 3435
Size: 152 3283
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1612 1644 2512 2544 2604 2636 3435
Size: 1612 32 868 32 60 32 799
Fragments arranged by size:
1612 868 799 60 32 32 32
HincII GTy'rAC
Cuts at: 0 1569 2781 3435
Size: 1569 1212 654
Fragments arranged by size:
1569 1212 654
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1412 3435
Size: 1412 2023
MscI TGG'CCA
Cuts at: 0 1974 3435
Size: 1974 1461
MslI CAynn'nnrTG
Cuts at: 0 223 2065 2075 3435
Size: 223 1842 10 1360
Fragments arranged by size:
1842 1360 223 10
MspA1I CmG'CkG
Cuts at: 0 1500 1970 3435
Size: 1500 470 1465
Fragments arranged by size:
1500 1465 470
NdeI CA'TA_TG
Cuts at: 0 2454 2763 3435
Size: 2454 309 672
Fragments arranged by size:
2454 672 309
NsiI A_TGCA'T
Cuts at: 0 1022 2577 3435
Size: 1022 1555 858
Fragments arranged by size:
1555 1022 858
NspI r_CATG'y
Cuts at: 0 200 898 1024 3435
Size: 200 698 126 2411
Fragments arranged by size:
2411 698 200 126
PasI CC'CwG_GG
Cuts at: 0 1040 1700 3435
Size: 1040 660 1735
Fragments arranged by size:
1735 1040 660
PciI A'CATG_T
Cuts at: 0 196 3435
Size: 196 3239
PfoI T'CCnGG_A
Cuts at: 0 690 1200 3435
Size: 690 510 2235
Fragments arranged by size:
2235 690 510
PpuMI rG'GwC_Cy
Cuts at: 0 1696 3435
Size: 1696 1739
PsiI TTA'TAA
Cuts at: 0 1238 1442 1589 2584 3435
Size: 1238 204 147 995 851
Fragments arranged by size:
1238 995 851 204 147
PstI C_TGCA'G
Cuts at: 0 2077 2913 3025 3435
Size: 2077 836 112 410
Fragments arranged by size:
2077 836 410 112
PvuII CAG'CTG
Cuts at: 0 1970 3435
Size: 1970 1465
SacII CC_GC'GG
Cuts at: 0 1501 3435
Size: 1501 1934
ScaI AGT'ACT
Cuts at: 0 2740 3435
Size: 2740 695
SfcI C'TryA_G
Cuts at: 0 534 2073 2909 3021 3435
Size: 534 1539 836 112 414
Fragments arranged by size:
1539 836 534 414 112
SspI AAT'ATT
Cuts at: 0 404 498 528 1153 3050 3435
Size: 404 94 30 625 1897 385
Fragments arranged by size:
1897 625 404 385 94 30
StuI AGG'CCT
Cuts at: 0 1035 1177 2132 3435
Size: 1035 142 955 1303
Fragments arranged by size:
1303 1035 955 142
StyI C'CwwG_G
Cuts at: 0 2918 3435
Size: 2918 517
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2343 3435
Size: 2343 1092
TatI w'GTAC_w
Cuts at: 0 379 448 2219 2738 3001 3435
Size: 379 69 1771 519 263 434
Fragments arranged by size:
1771 519 434 379 263 69
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1785 1817 1931 1963 3435
Size: 1785 32 114 32 1472
Fragments arranged by size:
1785 1472 114 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 977 3435
Size: 977 2458
XmnI GAAnn'nnTTC
Cuts at: 0 431 2035 3435
Size: 431 1604 1400
Fragments arranged by size:
1604 1400 431
ZraI GAC'GTC
Cuts at: 0 3186 3435
Size: 3186 249
Enzymes that do cut:
AatII AclI AcuI AfeI AflIII AleI AlfI ApaLI
ApoI AseI AvaI BamHI BanI BanII BbsI BcgI
BclI BfrBI BglII Bme1580I BmrI BplI BpmI Bpu10I
BsaI BsaHI BsaXI BseRI BsgI BsiHKAI BsmI BsmBI
Bsp1286I BsrDI BsrGI BssSI BstYI Bsu36I BtgI BtsI
CspCI DraI DraIII EaeI EarI Eco57MI EcoO109I EcoRI
FalI HaeII Hin4I HincII MmeI MscI MslI MspA1I
NdeI NsiI NspI PasI PciI PfoI PpuMI PsiI
PstI PvuII SacII ScaI SfcI SspI StuI StyI
TaqII TatI TstI XcmI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AflII AgeI AhdI AloI AlwNI
ApaI AscI AsiSI AvrII BaeI BbeI BbvCI BciVI
BglI BlpI BmgBI BmtI BpuEI BsaAI BsaBI BsaWI
BseYI BsiEI BsiWI BspEI BspHI BspMI BsrBI BsrFI
BssHII BstAPI BstBI BstEII BstXI BstZ17I BtgZI ClaI
DrdI EagI EciI EcoICRI EcoNI EcoRV FseI FspI
FspAI HindIII HpaI KasI KpnI MfeI MluI NaeI
NarI NcoI NgoMIV NheI NotI NruI PacI PflMI
PmeI PmlI PpiI PshAI PspOMI PspXI PsrI PvuI
RsrII SacI SalI SanDI SapI SbfI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SwaI Tth111I XbaI XhoI XmaI