(Circular) (Six-base) MAPSORT of: pFN21AB4057.seq Check: 3787 from: 1 to: 6766 pFN21AB4057 6766 bp With 182 enzymes: SgfI * October 20, 2009 10:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3477 4576 278 Size: 53 83 186 936 1941 1099 2468 Fragments arranged by size: 2468 1941 1099 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2166 2658 3210 4109 1937 Size: 229 492 552 899 4594 Fragments arranged by size: 4594 899 552 492 229 Acc65I G'GTAC_C Cuts at: 4087 4087 Size: 6766 AclI AA'CG_TT Cuts at: 1563 4893 5266 1563 Size: 3330 373 3063 Fragments arranged by size: 3330 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2164 2636 3296 4043 4829 6213 6481 1632 Size: 532 472 660 747 786 1384 268 1917 Fragments arranged by size: 1917 1384 786 747 660 532 472 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6569 AflIII A'CryG_T Cuts at: 1203 5665 6642 1203 Size: 4462 977 1327 Fragments arranged by size: 4462 1327 977 AgeI A'CCGG_T Cuts at: 5597 5597 Size: 6766 AhdI GACnn_n'nnGTC Cuts at: 3232 5495 3232 Size: 2263 4503 AleI CACnn'nnGTG Cuts at: 1166 2279 1166 Size: 1113 5653 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6766 AlwNI CAG_nnn'CTG Cuts at: 3073 4509 6081 3073 Size: 1436 1572 3758 Fragments arranged by size: 3758 1572 1436 ApaI G_GGCC'C Cuts at: 3570 3700 3570 Size: 130 6636 ApaLI G'TGCA_C Cuts at: 3239 4823 5979 3239 Size: 1584 1156 4026 Fragments arranged by size: 4026 1584 1156 ApoI r'AATT_y Cuts at: 1493 2545 4075 4301 4453 6562 1493 Size: 1052 1530 226 152 2109 1697 Fragments arranged by size: 2109 1697 1530 1052 226 152 AseI AT'TA_AT Cuts at: 160 1032 5320 160 Size: 872 4288 1606 Fragments arranged by size: 4288 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6766 AvaI C'yCGr_G Cuts at: 1870 1943 3569 4091 1870 Size: 73 1626 522 4545 Fragments arranged by size: 4545 1626 522 73 BamHI G'GATC_C Cuts at: 2384 2568 3216 3432 3780 4096 4463 2384 Size: 184 648 216 348 316 367 4687 Fragments arranged by size: 4687 648 367 348 316 216 184 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2430 2871 3578 4087 5542 Size: 325 217 627 643 441 707 509 1455 Cuts at: 5542 618 Size: 1842 Fragments arranged by size: 1842 1455 707 643 627 509 441 325 217 BanII G_rGCy'C Cuts at: 1391 2516 3570 3700 3795 4085 1391 Size: 1125 1054 130 95 290 4072 Fragments arranged by size: 4072 1125 1054 290 130 95 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2833 3911 928 Size: 971 934 1078 3783 Fragments arranged by size: 3783 1078 971 934 BbvCI CC'TCA_GC Cuts at: 2354 2867 3504 3819 2354 Size: 513 637 315 5301 Fragments arranged by size: 5301 637 513 315 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4957 4991 1903 Size: 34 3020 34 3678 Fragments arranged by size: 3678 3020 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4658 5868 4658 Size: 1210 5556 BclI T'GATC_A Cuts at: 1548 1548 Size: 6766 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2122 2852 5377 Size: 107 122 71 631 605 450 730 2525 Cuts at: 5377 136 Size: 1525 Fragments arranged by size: 2525 1525 730 631 605 450 122 107 71 BglII A'GATC_T Cuts at: 6761 6761 Size: 6766 BlpI GC'TnA_GC Cuts at: 4183 4183 Size: 6766 Bme1580I G_kGCm'C Cuts at: 1790 2435 3243 3538 3570 3700 4827 5983 1790 Size: 645 808 295 32 130 1127 1156 2573 Fragments arranged by size: 2573 1156 1127 808 645 295 130 32 BmgBI CAC'GTC Cuts at: 1318 3205 1318 Size: 1887 4879 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3116 4497 5455 Size: 416 53 83 399 24 1699 1381 958 Cuts at: 5455 442 Size: 1753 Fragments arranged by size: 1753 1699 1381 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6766 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3367 3631 3648 3367 Size: 264 17 6485 Fragments arranged by size: 6485 264 17 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2060 2066 2381 3626 3776 5426 1239 Size: 821 6 315 1245 150 1650 2579 Fragments arranged by size: 2579 1650 1245 821 315 150 6 Bpu10I CC'TnA_GC Cuts at: 2354 2867 3155 3504 3819 2354 Size: 513 288 349 315 5301 Fragments arranged by size: 5301 513 349 315 288 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4891 5756 6054 4891 Size: 865 298 5603 Fragments arranged by size: 5603 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2042 2055 5429 882 Size: 617 543 13 3374 2219 Fragments arranged by size: 3374 2219 617 543 13 BsaAI yAC'GTr Cuts at: 493 1204 2662 4557 493 Size: 711 1458 1895 2702 Fragments arranged by size: 2702 1895 1458 711 BsaBI GATnn'nnATC Cuts at: 3779 6760 3779 Size: 2981 3785 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2748 3474 4573 4955 Size: 53 83 186 936 1215 726 1099 382 Cuts at: 4955 5590 275 Size: 635 1451 Fragments arranged by size: 1451 1215 1099 936 726 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3777 4041 5199 5597 5871 6018 6429 3777 Size: 264 1158 398 274 147 411 4114 Fragments arranged by size: 4114 1158 411 398 274 264 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6541 Fragments arranged by size: 6541 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2027 2270 2480 3371 3425 3521 3692 3911 Size: 841 243 210 891 54 96 171 219 Cuts at: 3911 1186 Size: 4041 Fragments arranged by size: 4041 891 841 243 219 210 171 96 54 BseYI C'CCAG_C Cuts at: 2197 2398 2434 2490 2716 2724 2835 3012 5969 Size: 201 36 56 226 8 111 177 2957 Cuts at: 5969 6465 2197 Size: 496 2498 Fragments arranged by size: 2957 2498 496 226 201 177 111 56 36 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2117 2281 2842 3847 1163 Size: 573 381 164 561 1005 4082 Fragments arranged by size: 4082 1005 573 561 381 164 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2076 2629 4216 4977 6005 1540 Size: 275 186 75 553 1587 761 1028 2301 Fragments arranged by size: 2301 1587 1028 761 553 275 186 75 BsiHKAI G_wGCw'C Cuts at: 3161 3243 4827 4912 5983 3161 Size: 82 1584 85 1071 3944 Fragments arranged by size: 3944 1584 1071 85 82 BsmI GAATG_Cn' Cuts at: 4277 4370 4277 Size: 93 6673 BsmBI CGTCTCn'nnnn_ Cuts at: 3297 3297 Size: 6766 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2435 2516 3161 3243 3538 3570 3700 Size: 399 645 81 645 82 295 32 130 Cuts at: 3700 3795 4085 4827 4912 5983 1391 Size: 95 290 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 645 645 399 295 290 130 95 85 82 81 32 BspEI T'CCGG_A Cuts at: 3777 4041 6429 3777 Size: 264 2388 4114 Fragments arranged by size: 4114 2388 264 BspHI T'CATG_A Cuts at: 4655 6385 4655 Size: 1730 5036 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3051 3597 3738 3972 4121 844 Size: 2207 546 141 234 149 3489 Fragments arranged by size: 3489 2207 546 234 149 141 BsrBI CCG'CTC Cuts at: 1394 1823 2470 3310 3841 3973 4653 1394 Size: 429 647 840 531 132 680 3507 Fragments arranged by size: 3507 840 680 647 531 429 132 BsrDI GCAATG_nn' Cuts at: 65 1248 2962 5261 5435 65 Size: 1183 1714 2299 174 1396 Fragments arranged by size: 2299 1714 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2192 2624 3884 5410 5597 1700 Size: 110 382 432 1260 1526 187 2869 Fragments arranged by size: 2869 1526 1260 432 382 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6766 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6766 BssSI C'ACGA_G Cuts at: 3704 4826 5838 3704 Size: 1122 1012 4632 Fragments arranged by size: 4632 1122 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6766 BstBI TT'CG_AA Cuts at: 5570 5570 Size: 6766 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6766 BstXI CCAn_nnnn'nTGG Cuts at: 2281 2281 Size: 6766 BstYI r'GATC_y Cuts at: 1972 2384 2568 2970 3216 3432 3558 3774 3780 Size: 412 184 402 246 216 126 216 6 Cuts at: 3780 3915 4096 4463 4848 4865 6317 6403 6415 Size: 135 181 367 385 17 1452 86 12 Cuts at: 6415 6761 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 412 402 385 367 346 246 216 216 184 181 135 126 86 17 12 6 BtgI C'CryG_G Cuts at: 513 1065 2004 2277 2803 2850 3927 513 Size: 552 939 273 526 47 1077 3352 Fragments arranged by size: 3352 1077 939 552 526 273 47 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6027 Fragments arranged by size: 6027 683 56 BtsI GCAGTG_nn' Cuts at: 3007 3469 4282 5076 5096 3007 Size: 462 813 794 20 4677 Fragments arranged by size: 4677 813 794 462 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6766 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6731 DraI TTT'AAA Cuts at: 4070 4070 Size: 6766 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3473 5773 809 Size: 507 2157 2300 1802 Fragments arranged by size: 2300 2157 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2056 2073 Size: 54 1425 50 275 18 177 49 17 Cuts at: 2073 2325 2517 2626 2827 3399 3423 4213 5102 Size: 252 192 109 201 572 24 790 889 Cuts at: 5102 6731 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 790 572 275 252 201 192 177 109 54 50 49 43 24 18 17 EagI C'GGCC_G Cuts at: 1537 1812 2073 2626 4213 1537 Size: 275 261 553 1587 4090 Fragments arranged by size: 4090 1587 553 275 261 EarI CTCTTCn'nnn_ Cuts at: 1353 2423 2457 3089 3617 4696 1353 Size: 1070 34 632 528 1079 3423 Fragments arranged by size: 3423 1079 1070 632 528 34 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5353 5727 5873 387 Size: 1064 138 3764 374 146 1280 Fragments arranged by size: 3764 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2060 2066 2164 2381 2636 3296 3626 Size: 393 428 6 98 217 255 660 330 Cuts at: 3626 3776 4043 4829 5426 6213 6481 1239 Size: 150 267 786 597 787 268 1524 Fragments arranged by size: 1524 787 786 660 597 428 393 330 268 267 255 217 150 98 6 EcoNI CCTnn'n_nnAGG Cuts at: 3373 3686 3893 3373 Size: 313 207 6246 Fragments arranged by size: 6246 313 207 EcoO109I rG'GnC_Cy Cuts at: 3070 3348 3566 3697 3745 3070 Size: 278 218 131 48 6091 Fragments arranged by size: 6091 278 218 131 48 EcoRI G'AATT_C Cuts at: 4075 4453 4075 Size: 378 6388 EcoRV GAT'ATC Cuts at: 3319 3319 Size: 6766 FseI GG_CCGG'CC Cuts at: 3888 3888 Size: 6766 FspI TGC'GCA Cuts at: 5272 5272 Size: 6766 HaeII r_GCGC'y Cuts at: 1678 2794 5913 1678 Size: 1116 3119 2531 Fragments arranged by size: 3119 2531 1116 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2020 2052 3218 3250 5407 Size: 32 135 32 448 32 1166 32 2157 Cuts at: 5407 5439 5481 5513 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2157 1166 448 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2602 2689 4110 4356 669 Size: 988 281 664 87 1421 246 3079 Fragments arranged by size: 3079 1421 988 664 281 246 87 HindIII A'AGCT_T Cuts at: 748 748 Size: 6766 HpaI GTT'AAC Cuts at: 4356 4356 Size: 6766 KpnI G_GTAC'C Cuts at: 4091 4091 Size: 6766 MfeI C'AATT_G Cuts at: 4365 4365 Size: 6766 MluI A'CGCG_T Cuts at: 6642 6642 Size: 6766 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3702 5880 6064 1305 Size: 2397 2178 184 2007 Fragments arranged by size: 2397 2178 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2829 3425 10 Size: 54 1425 343 997 596 3351 Fragments arranged by size: 3351 1425 997 596 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2279 3455 3689 4724 5083 5242 518 Size: 648 1113 1176 234 1035 359 159 2042 Fragments arranged by size: 2042 1176 1113 1035 648 359 234 159 MspA1I CmG'CkG Cuts at: 1606 2438 3503 4182 4859 6007 6252 1606 Size: 832 1065 679 677 1148 245 2120 Fragments arranged by size: 2120 1148 1065 832 679 677 245 NaeI GCC'GGC Cuts at: 2626 3886 2626 Size: 1260 5506 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5275 Fragments arranged by size: 5275 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6766 NgoMIV G'CCGG_C Cuts at: 2624 3884 2624 Size: 1260 5506 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6766 NotI GC'GGCC_GC Cuts at: 4213 4213 Size: 6766 NspI r_CATG'y Cuts at: 1132 1229 4124 5669 1132 Size: 97 2895 1545 2229 Fragments arranged by size: 2895 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6766 PciI A'CATG_T Cuts at: 5665 5665 Size: 6766 PflMI CCAn_nnn'nTGG Cuts at: 1108 2763 3994 1108 Size: 1655 1231 3880 Fragments arranged by size: 3880 1655 1231 PfoI T'CCnGG_A Cuts at: 2762 2762 Size: 6766 PmeI GTTT'AAAC Cuts at: 4070 4070 Size: 6766 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4835 4867 6348 6380 4835 Size: 32 1481 32 5221 Fragments arranged by size: 5221 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3348 3745 3348 Size: 397 6369 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6766 PsiI TTA'TAA Cuts at: 4336 4336 Size: 6766 PspOMI G'GGCC_C Cuts at: 3566 3696 3566 Size: 130 6636 PstI C_TGCA'G Cuts at: 830 2313 3048 3834 4118 830 Size: 1483 735 786 284 3478 Fragments arranged by size: 3478 1483 786 735 284 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6766 SalI G'TCGA_C Cuts at: 1936 4108 1936 Size: 2172 4594 SapI GCTCTTCn'nnn_ Cuts at: 2423 3089 3617 2423 Size: 666 528 5572 Fragments arranged by size: 5572 666 528 SbfI CC_TGCA'GG Cuts at: 4118 4118 Size: 6766 ScaI AGT'ACT Cuts at: 2948 5014 6726 2948 Size: 2066 1712 2988 Fragments arranged by size: 2988 2066 1712 SfcI C'TryA_G Cuts at: 826 1045 2309 3044 3830 4114 5249 5930 6121 Size: 219 1264 735 786 284 1135 681 191 Cuts at: 6121 826 Size: 1471 Fragments arranged by size: 1471 1264 1135 786 735 681 284 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6766 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6766 SmaI CCC'GGG Cuts at: 1872 4093 1872 Size: 2221 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4870 5771 6033 820 Size: 197 926 2927 901 262 1553 Fragments arranged by size: 2927 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4557 493 Size: 4064 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6766 SphI G_CATG'C Cuts at: 1132 4124 1132 Size: 2992 3774 SspI AAT'ATT Cuts at: 5 52 4690 5 Size: 47 4638 2081 Fragments arranged by size: 4638 2081 47 StuI AGG'CCT Cuts at: 1517 2366 3368 1517 Size: 849 1002 4915 Fragments arranged by size: 4915 1002 849 StyI C'CwwG_G Cuts at: 513 1065 2004 2223 2540 3784 4205 513 Size: 552 939 219 317 1244 421 3074 Fragments arranged by size: 3074 1244 939 552 421 317 219 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2504 3004 3338 4793 4810 4963 5148 Size: 1255 924 500 334 1455 17 153 185 Cuts at: 5148 5593 325 Size: 445 1498 Fragments arranged by size: 1498 1455 1255 924 500 445 334 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2946 5012 6724 Size: 274 80 33 51 1443 969 2066 1712 Cuts at: 6724 96 Size: 138 Fragments arranged by size: 2066 1712 1443 969 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6726 6758 6726 Size: 32 6734 Tth111I GACn'n_nGTC Cuts at: 1316 2747 1316 Size: 1431 5335 XbaI T'CTAG_A Cuts at: 2593 4102 2593 Size: 1509 5257 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2395 3227 2395 Size: 832 5934 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6766 XmaI C'CCGG_G Cuts at: 1870 4091 1870 Size: 2221 4545 XmnI GAAnn'nnTTC Cuts at: 4037 4895 4037 Size: 858 5908 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3475 4574 276 Size: 53 83 186 936 1941 1099 2468 Fragments arranged by size: 2468 1941 1099 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoICRI FalI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI