Restriction map for pFN21AB4057
 (Linear) (Six-base) MAPSORT of: KIBB4057.seq  Check: 9000  from: 1  to: 2061



KIBB4057.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:02  ..

AatII G_ACGT'C

Cuts at:      0    1472    2061 
   Size:       1472     589
 
AccI GT'mk_AC

Cuts at:      0     161     653    1205    2061 
   Size:        161     492     552     856

  Fragments arranged by size: 

                 856     552     492     161
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     159     631    1291    2038    2061 
   Size:        159     472     660     747      23

  Fragments arranged by size: 

                 747     660     472     159      23
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1227    2061 
   Size:       1227     834
 
AleI CACnn'nnGTG

Cuts at:      0     274    2061 
   Size:        274    1787
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1068    2061 
   Size:       1068     993
 
ApaI G_GGCC'C

Cuts at:      0    1565    1695    2061 
   Size:       1565     130     366

  Fragments arranged by size: 

                1565     366     130
 
ApaLI G'TGCA_C

Cuts at:      0    1234    2061 
   Size:       1234     827
 
ApoI r'AATT_y

Cuts at:      0     540    2061 
   Size:        540    1521
 
AvaI C'yCGr_G

Cuts at:      0    1564    2061 
   Size:       1564     497
 
BamHI G'GATC_C

Cuts at:      0     379     563    1211    1427    1775    2061 
   Size:        379     184     648     216     348     286

  Fragments arranged by size: 

                 648     379     348     286     216     184
 
BanI G'GyrC_C

Cuts at:      0     425     866    1573    2061 
   Size:        425     441     707     488

  Fragments arranged by size: 

                 707     488     441     425
 
BanII G_rGCy'C

Cuts at:      0     511    1565    1695    1790    2061 
   Size:        511    1054     130      95     271

  Fragments arranged by size: 

                1054     511     271     130      95
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     828    1906    2061 
   Size:        828    1078     155

  Fragments arranged by size: 

                1078     828     155
 
BbvCI CC'TCA_GC

Cuts at:      0     349     862    1499    1814    2061 
   Size:        349     513     637     315     247

  Fragments arranged by size: 

                 637     513     349     315     247
 
BglI GCCn_nnn'nGGC

Cuts at:      0     117     847    2061 
   Size:        117     730    1214

  Fragments arranged by size: 

                1214     730     117
 
Bme1580I G_kGCm'C

Cuts at:      0     430    1238    1533    1565    1695    2061 
   Size:        430     808     295      32     130     366

  Fragments arranged by size: 

                 808     430     366     295     130      32
 
BmgBI CAC'GTC

Cuts at:      0    1200    2061 
   Size:       1200     861
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1111    2061 
   Size:       1111     950
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1362    1626    1643    2061 
   Size:       1362     264      17     418

  Fragments arranged by size: 

                1362     418     264      17
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      55      61     376    1621    1771    2061 
   Size:         55       6     315    1245     150     290

  Fragments arranged by size: 

                1245     315     290     150      55       6
 
Bpu10I CC'TnA_GC

Cuts at:      0     349     862    1150    1499    1814    2061 
   Size:        349     513     288     349     315     247

  Fragments arranged by size: 

                 513     349     349     315     288     247
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      37      50    2061 
   Size:         37      13    2011

  Fragments arranged by size: 

                2011      37      13
 
BsaAI yAC'GTr

Cuts at:      0     657    2061 
   Size:        657    1404
 
BsaBI GATnn'nnATC

Cuts at:      0    1774    2061 
   Size:       1774     287
 
BsaHI Gr'CG_yC

Cuts at:      0     743    1469    2061 
   Size:        743     726     592

  Fragments arranged by size: 

                 743     726     592
 
BsaWI w'CCGG_w

Cuts at:      0    1772    2036    2061 
   Size:       1772     264      25

  Fragments arranged by size: 

                1772     264      25
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      22     265     475    1366    1420    1516    1687    1906 
   Size:         22     243     210     891      54      96     171     219

Cuts at:   1906    2061 
   Size:        155

  Fragments arranged by size: 

                 891     243     219     210     171     155      96      54
                  22
 
BseYI C'CCAG_C

Cuts at:      0     192     393     429     485     711     719     830    1007 
   Size:        192     201      36      56     226       8     111     177

Cuts at:   1007    2061 
   Size:       1054

  Fragments arranged by size: 

                1054     226     201     192     177     111      56      36
                   8
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     112     276     837    1842    2061 
   Size:        112     164     561    1005     219

  Fragments arranged by size: 

                1005     561     219     164     112
 
BsiEI CG_ry'CG

Cuts at:      0      71     624    2061 
   Size:         71     553    1437

  Fragments arranged by size: 

                1437     553      71
 
BsiHKAI G_wGCw'C

Cuts at:      0    1156    1238    2061 
   Size:       1156      82     823

  Fragments arranged by size: 

                1156     823      82
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1292    2061 
   Size:       1292     769
 
Bsp1286I G_dGCh'C

Cuts at:      0     430     511    1156    1238    1533    1565    1695    1790 
   Size:        430      81     645      82     295      32     130      95

Cuts at:   1790    2061 
   Size:        271

  Fragments arranged by size: 

                 645     430     295     271     130      95      82      81
                  32
 
BspEI T'CCGG_A

Cuts at:      0    1772    2036    2061 
   Size:       1772     264      25

  Fragments arranged by size: 

                1772     264      25
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1046    1592    1733    1967    2061 
   Size:       1046     546     141     234      94

  Fragments arranged by size: 

                1046     546     234     141      94
 
BsrBI CCG'CTC

Cuts at:      0     465    1305    1836    1968    2061 
   Size:        465     840     531     132      93

  Fragments arranged by size: 

                 840     531     465     132      93
 
BsrDI GCAATG_nn'

Cuts at:      0     957    2061 
   Size:        957    1104
 
BsrFI r'CCGG_y

Cuts at:      0     187     619    1879    2061 
   Size:        187     432    1260     182

  Fragments arranged by size: 

                1260     432     187     182
 
BssSI C'ACGA_G

Cuts at:      0    1699    2061 
   Size:       1699     362
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     276    2061 
   Size:        276    1785
 
BstYI r'GATC_y

Cuts at:      0     379     563     965    1211    1427    1553    1769    1775 
   Size:        379     184     402     246     216     126     216       6

Cuts at:   1775    1910    2061 
   Size:        135     151

  Fragments arranged by size: 

                 402     379     246     216     216     184     151     135
                 126       6
 
BtgI C'CryG_G

Cuts at:      0     272     798     845    1922    2061 
   Size:        272     526      47    1077     139

  Fragments arranged by size: 

                1077     526     272     139      47
 
BtsI GCAGTG_nn'

Cuts at:      0    1002    1464    2061 
   Size:       1002     462     597

  Fragments arranged by size: 

                1002     597     462
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1468    2061 
   Size:       1468     593
 
EaeI y'GGCC_r

Cuts at:      0       2      51      68     320     512     621     822    1394 
   Size:          2      49      17     252     192     109     201     572

Cuts at:   1394    1418    2061 
   Size:         24     643

  Fragments arranged by size: 

                 643     572     252     201     192     109      49      24
                  17       2
 
EagI C'GGCC_G

Cuts at:      0      68     621    2061 
   Size:         68     553    1440

  Fragments arranged by size: 

                1440     553      68
 
EarI CTCTTCn'nnn_

Cuts at:      0     418     452    1084    1612    2061 
   Size:        418      34     632     528     449

  Fragments arranged by size: 

                 632     528     449     418      34
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      55      61     159     376     631    1291    1621    1771 
   Size:         55       6      98     217     255     660     330     150

Cuts at:   1771    2038    2061 
   Size:        267      23

  Fragments arranged by size: 

                 660     330     267     255     217     150      98      55
                  23       6
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1368    1681    1888    2061 
   Size:       1368     313     207     173

  Fragments arranged by size: 

                1368     313     207     173
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1065    1343    1561    1692    1740    2061 
   Size:       1065     278     218     131      48     321

  Fragments arranged by size: 

                1065     321     278     218     131      48
 
EcoRV GAT'ATC

Cuts at:      0    1314    2061 
   Size:       1314     747
 
FseI GG_CCGG'CC

Cuts at:      0    1883    2061 
   Size:       1883     178
 
HaeII r_GCGC'y

Cuts at:      0     789    2061 
   Size:        789    1272
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      15      47    1213    1245    2061 
   Size:         15      32    1166      32     816

  Fragments arranged by size: 

                1166     816      32      32      15
 
HincII GTy'rAC

Cuts at:      0     597     684    2061 
   Size:        597      87    1377

  Fragments arranged by size: 

                1377     597      87
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1697    2061 
   Size:       1697     364
 
MscI TGG'CCA

Cuts at:      0     824    1420    2061 
   Size:        824     596     641

  Fragments arranged by size: 

                 824     641     596
 
MslI CAynn'nnrTG

Cuts at:      0     274    1450    1684    2061 
   Size:        274    1176     234     377

  Fragments arranged by size: 

                1176     377     274     234
 
MspA1I CmG'CkG

Cuts at:      0     433    1498    2061 
   Size:        433    1065     563

  Fragments arranged by size: 

                1065     563     433
 
NaeI GCC'GGC

Cuts at:      0     621    1881    2061 
   Size:        621    1260     180

  Fragments arranged by size: 

                1260     621     180
 
NgoMIV G'CCGG_C

Cuts at:      0     619    1879    2061 
   Size:        619    1260     182

  Fragments arranged by size: 

                1260     619     182
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     758    1989    2061 
   Size:        758    1231      72

  Fragments arranged by size: 

                1231     758      72
 
PfoI T'CCnGG_A

Cuts at:      0     757    2061 
   Size:        757    1304
 
PpuMI rG'GwC_Cy

Cuts at:      0    1343    1740    2061 
   Size:       1343     397     321

  Fragments arranged by size: 

                1343     397     321
 
PspOMI G'GGCC_C

Cuts at:      0    1561    1691    2061 
   Size:       1561     130     370

  Fragments arranged by size: 

                1561     370     130
 
PstI C_TGCA'G

Cuts at:      0     308    1043    1829    2061 
   Size:        308     735     786     232

  Fragments arranged by size: 

                 786     735     308     232
 
SapI GCTCTTCn'nnn_

Cuts at:      0     418    1084    1612    2061 
   Size:        418     666     528     449

  Fragments arranged by size: 

                 666     528     449     418
 
ScaI AGT'ACT

Cuts at:      0     943    2061 
   Size:        943    1118
 
SfcI C'TryA_G

Cuts at:      0     304    1039    1825    2061 
   Size:        304     735     786     236

  Fragments arranged by size: 

                 786     735     304     236
 
StuI AGG'CCT

Cuts at:      0     361    1363    2061 
   Size:        361    1002     698

  Fragments arranged by size: 

                1002     698     361
 
StyI C'CwwG_G

Cuts at:      0     218     535    1779    2061 
   Size:        218     317    1244     282

  Fragments arranged by size: 

                1244     317     282     218
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     499     999    1333    2061 
   Size:        499     500     334     728

  Fragments arranged by size: 

                 728     500     499     334
 
TatI w'GTAC_w

Cuts at:      0     941    2061 
   Size:        941    1120
 
Tth111I GACn'n_nGTC

Cuts at:      0     742    2061 
   Size:        742    1319
 
XbaI T'CTAG_A

Cuts at:      0     588    2061 
   Size:        588    1473
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     390    1222    2061 
   Size:        390     832     839

  Fragments arranged by size: 

                 839     832     390
 
XmnI GAAnn'nnTTC

Cuts at:      0    2032    2061 
   Size:       2032      29
 
ZraI GAC'GTC

Cuts at:      0    1470    2061 
   Size:       1470     591
 

 Enzymes that do cut:

    AatII     AccI     AcuI     AhdI     AleI    AlwNI     ApaI    ApaLI
     ApoI     AvaI    BamHI     BanI    BanII     BbsI    BbvCI     BglI
 Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsmBI Bsp1286I    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BssSI
    BstXI    BstYI     BtgI     BtsI     DrdI     EaeI     EagI     EarI
  Eco57MI    EcoNI EcoO109I    EcoRV     FseI    HaeII    Hin4I   HincII
     MmeI     MscI     MslI   MspA1I     NaeI   NgoMIV    PflMI     PfoI
    PpuMI   PspOMI     PstI     SapI     ScaI     SfcI     StuI     StyI
    TaqII     TatI  Tth111I     XbaI     XcmI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AlfI
     AloI     AscI     AseI    AsiSI    AvrII     BaeI     BbeI     BcgI
    BciVI     BclI    BfrBI    BglII     BlpI     BmtI    BpuEI    BsaXI
    BsiWI     BsmI    BspHI    BsrGI   BssHII   BstAPI    BstBI   BstEII
  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     EciI
  EcoICRI    EcoRI     FalI     FspI    FspAI  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     NarI     NcoI     NdeI     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI     PmeI     PmlI
     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SbfI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI     TstI     XhoI     XmaI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com