Restriction map for
pFN21AB3472
(Circular) (Six-base) MAPSORT of: pFN21AB3472.seq Check: 5349 from: 1 to: 7054
pFN21AB3472 7054 bp
With 182 enzymes: SgfI *
October 20, 2009 10:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4864 278
Size: 53 83 186 936 3328 2468
Fragments arranged by size:
3328 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3876 4397 1937
Size: 1939 521 4594
Fragments arranged by size:
4594 1939 521
Acc65I G'GTAC_C
Cuts at: 4375 4375
Size: 7054
AclI AA'CG_TT
Cuts at: 1563 5181 5554 1563
Size: 3618 373 3063
Fragments arranged by size:
3618 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2148 2220 2474 2607 2827 3120 4159 4189
Size: 516 72 254 133 220 293 1039 30
Cuts at: 4189 5117 6501 6769 1632
Size: 928 1384 268 1917
Fragments arranged by size:
1917 1384 1039 928 516 293 268 254
220 133 72 30
AfeI AGC'GCT
Cuts at: 3884 3884
Size: 7054
AflII C'TTAA_G
Cuts at: 820 1017 3002 820
Size: 197 1985 4872
Fragments arranged by size:
4872 1985 197
AflIII A'CryG_T
Cuts at: 1203 5953 6930 1203
Size: 4750 977 1327
Fragments arranged by size:
4750 1327 977
AgeI A'CCGG_T
Cuts at: 5885 5885
Size: 7054
AhdI GACnn_n'nnGTC
Cuts at: 5783 5783
Size: 7054
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7054
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7054
AlwNI CAG_nnn'CTG
Cuts at: 2705 2794 3364 4797 6369 2705
Size: 89 570 1433 1572 3390
Fragments arranged by size:
3390 1572 1433 570 89
ApaLI G'TGCA_C
Cuts at: 5111 6267 5111
Size: 1156 5898
ApoI r'AATT_y
Cuts at: 1493 2212 3373 4363 4589 4741 6850 1493
Size: 719 1161 990 226 152 2109 1697
Fragments arranged by size:
2109 1697 1161 990 719 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5608 160
Size: 872 4576 1606
Fragments arranged by size:
4576 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7054
AvaI C'yCGr_G
Cuts at: 1870 1943 4264 4379 1870
Size: 73 2321 115 4545
Fragments arranged by size:
4545 2321 115 73
BamHI G'GATC_C
Cuts at: 4384 4751 4384
Size: 367 6687
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3863 4375 5830 618
Size: 325 217 627 2076 512 1455 1842
Fragments arranged by size:
2076 1842 1455 627 512 325 217
BanII G_rGCy'C
Cuts at: 1391 2307 4373 1391
Size: 916 2066 4072
Fragments arranged by size:
4072 2066 916
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2717 3108 928
Size: 971 818 391 4874
Fragments arranged by size:
4874 971 818 391
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5245 5279 1903
Size: 34 50 34 3224 34 3678
Fragments arranged by size:
3678 3224 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4946 6156 4946
Size: 1210 5844
BclI T'GATC_A
Cuts at: 1548 3055 3891 1548
Size: 1507 836 4711
Fragments arranged by size:
4711 1507 836
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5665 136
Size: 107 122 71 631 605 3993 1525
Fragments arranged by size:
3993 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2989 7049 2989
Size: 4060 2994
BlpI GC'TnA_GC
Cuts at: 4471 4471
Size: 7054
Bme1580I G_kGCm'C
Cuts at: 1790 2785 5115 6271 1790
Size: 995 2330 1156 2573
Fragments arranged by size:
2573 2330 1156 995
BmgBI CAC'GTC
Cuts at: 1318 3870 1318
Size: 2552 4502
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2487 2715 4785
Size: 416 53 83 399 24 1070 228 2070
Cuts at: 4785 5743 442
Size: 958 1753
Fragments arranged by size:
2070 1753 1070 958 416 399 228 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7054
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5714 1239
Size: 4475 2579
Bpu10I CC'TnA_GC
Cuts at: 2388 2388
Size: 7054
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5179 6044 6342 5179
Size: 865 298 5891
Fragments arranged by size:
5891 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5717 882
Size: 617 4218 2219
Fragments arranged by size:
4218 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3500 4845 493
Size: 711 2296 1345 2702
Fragments arranged by size:
2702 2296 1345 711
BsaBI GATnn'nnATC
Cuts at: 2933 7048 2933
Size: 4115 2939
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4861 5243 5878 275
Size: 53 83 186 936 3328 382 635 1451
Fragments arranged by size:
3328 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2479 5487 5885 6159 6306 6717 2479
Size: 3008 398 274 147 411 2816
Fragments arranged by size:
3008 2816 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3462 3492 3869 3899 4321
Size: 30 165 30 2059 30 377 30 422
Cuts at: 4321 4351 1178
Size: 30 3881
Fragments arranged by size:
3881 2059 422 377 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2102 2261 2372 2631 3827 4007 1186
Size: 916 159 111 259 1196 180 4233
Fragments arranged by size:
4233 1196 916 259 180 159 111
BseYI C'CCAG_C
Cuts at: 3985 4039 6257 6753 3985
Size: 54 2218 496 4286
Fragments arranged by size:
4286 2218 496 54
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3480 1163
Size: 573 1744 4737
Fragments arranged by size:
4737 1744 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4504 5265 6293 1540
Size: 275 186 2503 761 1028 2301
Fragments arranged by size:
2503 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3935 5115 5200 6271 3935
Size: 1180 85 1071 4718
Fragments arranged by size:
4718 1180 1071 85
BsmI GAATG_Cn'
Cuts at: 4565 4658 4565
Size: 93 6961
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2307 2785 3935 4373 5115 5200 6271
Size: 399 517 478 1150 438 742 85 1071
Cuts at: 6271 1391
Size: 2174
Fragments arranged by size:
2174 1150 1071 742 517 478 438 399
85
BspEI T'CCGG_A
Cuts at: 6717 6717
Size: 7054
BspHI T'CATG_A
Cuts at: 4943 6673 4943
Size: 1730 5324
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4409 844
Size: 3565 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4941 1394
Size: 429 3118 3507
Fragments arranged by size:
3507 3118 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2058 3116 5549 5723 65
Size: 1183 810 1058 2433 174 1396
Fragments arranged by size:
2433 1396 1183 1058 810 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5698 5885 1700
Size: 110 3888 187 2869
Fragments arranged by size:
3888 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7054
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7054
BssSI C'ACGA_G
Cuts at: 5114 6126 5114
Size: 1012 6042
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7054
BstBI TT'CG_AA
Cuts at: 5858 5858
Size: 7054
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7054
BstYI r'GATC_y
Cuts at: 1972 2989 4304 4384 4751 5136 5153 6605 6691
Size: 1017 1315 80 367 385 17 1452 86
Cuts at: 6691 6703 7049 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1315 1017 385 367 346 86
80 17 12
Bsu36I CC'TnA_GG
Cuts at: 2787 3516 2787
Size: 729 6325
BtgI C'CryG_G
Cuts at: 513 1065 4154 513
Size: 552 3089 3413
Fragments arranged by size:
3413 3089 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6315
Fragments arranged by size:
6315 683 56
BtsI GCAGTG_nn'
Cuts at: 2069 3768 3935 4570 5364 5384 2069
Size: 1699 167 635 794 20 3739
Fragments arranged by size:
3739 1699 794 635 167 20
ClaI AT'CG_AT
Cuts at: 1554 3574 1554
Size: 2020 5034
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3216 3251 529
Size: 35 2652 35 4332
Fragments arranged by size:
4332 2652 35 35
DraI TTT'AAA
Cuts at: 4358 4358
Size: 7054
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6061 809
Size: 507 4745 1802
Fragments arranged by size:
4745 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4125 4501 5390
Size: 54 1425 50 275 18 2295 376 889
Cuts at: 5390 7019 8
Size: 1629 43
Fragments arranged by size:
2295 1629 1425 889 376 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4501 1537
Size: 275 2689 4090
Fragments arranged by size:
4090 2689 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2124 2136 2765 3060 4984 1353
Size: 771 12 629 295 1924 3423
Fragments arranged by size:
3423 1924 771 629 295 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3598 5641 6015 6161 387
Size: 1064 138 2009 2043 374 146 1280
Fragments arranged by size:
2043 2009 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2148 2220 2474 2607 2827 3120 4159
Size: 393 516 72 254 133 220 293 1039
Cuts at: 4159 4189 5117 5714 6501 6769 1239
Size: 30 928 597 787 268 1524
Fragments arranged by size:
1524 1039 928 787 597 516 393 293
268 254 220 133 72 30
EcoRI G'AATT_C
Cuts at: 2212 3373 4363 4741 2212
Size: 1161 990 378 4525
Fragments arranged by size:
4525 1161 990 378
EcoRV GAT'ATC
Cuts at: 2935 2935
Size: 7054
FspI TGC'GCA
Cuts at: 5560 5560
Size: 7054
HaeII r_GCGC'y
Cuts at: 1678 2565 3886 6201 1678
Size: 887 1321 2315 2531
Fragments arranged by size:
2531 2315 1321 887
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2237 2269 2641 2673 3312
Size: 32 135 32 665 32 372 32 639
Cuts at: 3312 3344 3383 3415 3545 3577 3869 3901 5695
Size: 32 39 32 130 32 292 32 1794
Cuts at: 5695 5727 5769 5801 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1794 665 639 372 292 135 130
42 39 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2206 4398 4644 669
Size: 988 281 268 2192 246 3079
Fragments arranged by size:
3079 2192 988 281 268 246
HindIII A'AGCT_T
Cuts at: 748 3723 748
Size: 2975 4079
HpaI GTT'AAC
Cuts at: 4644 4644
Size: 7054
KpnI G_GTAC'C
Cuts at: 4379 4379
Size: 7054
MfeI C'AATT_G
Cuts at: 3936 4653 3936
Size: 717 6337
MluI A'CGCG_T
Cuts at: 6930 6930
Size: 7054
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2923 3018 6168 6352 1305
Size: 1618 95 3150 184 2007
Fragments arranged by size:
3150 2007 1618 184 95
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4127 10
Size: 54 1425 343 2295 2937
Fragments arranged by size:
2937 2295 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2051 5012 5371 5530 518
Size: 648 885 2961 359 159 2042
Fragments arranged by size:
2961 2042 885 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2670 3797 3985 4186 4216 4246 4470 5147
Size: 1064 1127 188 201 30 30 224 677
Cuts at: 5147 6295 6540 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 1127 1064 677 245 224 201
188 30 30
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6502
NdeI CA'TA_TG
Cuts at: 387 3184 387
Size: 2797 4257
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7054
NotI GC'GGCC_GC
Cuts at: 4501 4501
Size: 7054
NspI r_CATG'y
Cuts at: 1132 1229 3011 4412 5957 1132
Size: 97 1782 1401 1545 2229
Fragments arranged by size:
2229 1782 1545 1401 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7054
PciI A'CATG_T
Cuts at: 5953 5953
Size: 7054
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3037 1108
Size: 1929 5125
PfoI T'CCnGG_A
Cuts at: 3015 3015
Size: 7054
PmeI GTTT'AAAC
Cuts at: 4358 4358
Size: 7054
PmlI CAC'GTG
Cuts at: 3500 3500
Size: 7054
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5123 5155 6636 6668 5123
Size: 32 1481 32 5509
Fragments arranged by size:
5509 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7054
PsiI TTA'TAA
Cuts at: 4624 4624
Size: 7054
PstI C_TGCA'G
Cuts at: 830 4406 830
Size: 3576 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7054
PvuII CAG'CTG
Cuts at: 3797 3985 3797
Size: 188 6866
SalI G'TCGA_C
Cuts at: 1936 4396 1936
Size: 2460 4594
SbfI CC_TGCA'GG
Cuts at: 4406 4406
Size: 7054
ScaI AGT'ACT
Cuts at: 5302 7014 5302
Size: 1712 5342
SexAI A'CCwGG_T
Cuts at: 2950 2950
Size: 7054
SfcI C'TryA_G
Cuts at: 826 1045 4402 5537 6218 6409 826
Size: 219 3357 1135 681 191 1471
Fragments arranged by size:
3357 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7054
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7054
SmaI CCC'GGG
Cuts at: 1872 4381 1872
Size: 2509 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3002 5158 6059 6321 820
Size: 197 926 1059 2156 901 262 1553
Fragments arranged by size:
2156 1553 1059 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4845 493
Size: 4352 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7054
SphI G_CATG'C
Cuts at: 1132 4412 1132
Size: 3280 3774
SspI AAT'ATT
Cuts at: 5 52 3952 4978 5
Size: 47 3900 1026 2081
Fragments arranged by size:
3900 2081 1026 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7054
StyI C'CwwG_G
Cuts at: 513 1065 4493 513
Size: 552 3428 3074
Fragments arranged by size:
3428 3074 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4031 5081 5098 5251 5436 5881 325
Size: 1255 2451 1050 17 153 185 445 1498
Fragments arranged by size:
2451 1498 1255 1050 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3652 5300 7012
Size: 274 80 33 51 1443 1675 1648 1712
Cuts at: 7012 96
Size: 138
Fragments arranged by size:
1712 1675 1648 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3462 3494 7014 7046 3462
Size: 32 3520 32 3470
Fragments arranged by size:
3520 3470 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7054
XbaI T'CTAG_A
Cuts at: 4390 4390
Size: 7054
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2975 3748 2975
Size: 773 6281
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7054
XmaI C'CCGG_G
Cuts at: 1870 4379 1870
Size: 2509 4545
XmnI GAAnn'nnTTC
Cuts at: 2714 5183 2714
Size: 2469 4585
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4862 276
Size: 53 83 186 936 3328 2468
Fragments arranged by size:
3328 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI
EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SfiI SfoI SrfI
SwaI