Restriction map for
pFN21AB3472
(Linear) (Six-base) MAPSORT of: KIBB3472.seq Check: 1962 from: 1 to: 2349
KIBB3472.orf
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AccI GT'mk_AC
Cuts at: 0 1871 2349
Size: 1871 478
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 143 215 469 602 822 1115 2154 2184
Size: 143 72 254 133 220 293 1039 30
Cuts at: 2184 2349
Size: 165
Fragments arranged by size:
1039 293 254 220 165 143 133 72
30
AfeI AGC'GCT
Cuts at: 0 1879 2349
Size: 1879 470
AflII C'TTAA_G
Cuts at: 0 997 2349
Size: 997 1352
AlwNI CAG_nnn'CTG
Cuts at: 0 700 789 1359 2349
Size: 700 89 570 990
Fragments arranged by size:
990 700 570 89
ApoI r'AATT_y
Cuts at: 0 207 1368 2349
Size: 207 1161 981
Fragments arranged by size:
1161 981 207
AvaI C'yCGr_G
Cuts at: 0 2259 2349
Size: 2259 90
BanI G'GyrC_C
Cuts at: 0 1858 2349
Size: 1858 491
BanII G_rGCy'C
Cuts at: 0 302 2349
Size: 302 2047
BbsI GAAGACnn'nnnn_
Cuts at: 0 712 1103 2349
Size: 712 391 1246
Fragments arranged by size:
1246 712 391
BclI T'GATC_A
Cuts at: 0 1050 1886 2349
Size: 1050 836 463
Fragments arranged by size:
1050 836 463
BglII A'GATC_T
Cuts at: 0 984 2349
Size: 984 1365
Bme1580I G_kGCm'C
Cuts at: 0 780 2349
Size: 780 1569
BmgBI CAC'GTC
Cuts at: 0 1865 2349
Size: 1865 484
BmrI ACTGGGnnnn_n'
Cuts at: 0 482 710 2349
Size: 482 228 1639
Fragments arranged by size:
1639 482 228
Bpu10I CC'TnA_GC
Cuts at: 0 383 2349
Size: 383 1966
BsaAI yAC'GTr
Cuts at: 0 1495 2349
Size: 1495 854
BsaBI GATnn'nnATC
Cuts at: 0 928 2349
Size: 928 1421
BsaWI w'CCGG_w
Cuts at: 0 474 2349
Size: 474 1875
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1457 1487 1864 1894 2316 2346 2349
Size: 1457 30 377 30 422 30 3
Fragments arranged by size:
1457 422 377 30 30 30 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 97 256 367 626 1822 2002 2349
Size: 97 159 111 259 1196 180 347
Fragments arranged by size:
1196 347 259 180 159 111 97
BseYI C'CCAG_C
Cuts at: 0 1980 2034 2349
Size: 1980 54 315
Fragments arranged by size:
1980 315 54
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1475 2349
Size: 1475 874
BsiHKAI G_wGCw'C
Cuts at: 0 1930 2349
Size: 1930 419
Bsp1286I G_dGCh'C
Cuts at: 0 302 780 1930 2349
Size: 302 478 1150 419
Fragments arranged by size:
1150 478 419 302
BsrDI GCAATG_nn'
Cuts at: 0 53 1111 2349
Size: 53 1058 1238
Fragments arranged by size:
1238 1058 53
BstYI r'GATC_y
Cuts at: 0 984 2299 2349
Size: 984 1315 50
Fragments arranged by size:
1315 984 50
Bsu36I CC'TnA_GG
Cuts at: 0 782 1511 2349
Size: 782 729 838
Fragments arranged by size:
838 782 729
BtgI C'CryG_G
Cuts at: 0 2149 2349
Size: 2149 200
BtsI GCAGTG_nn'
Cuts at: 0 64 1763 1930 2349
Size: 64 1699 167 419
Fragments arranged by size:
1699 419 167 64
ClaI AT'CG_AT
Cuts at: 0 1569 2349
Size: 1569 780
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1211 1246 2349
Size: 1211 35 1103
Fragments arranged by size:
1211 1103 35
EaeI y'GGCC_r
Cuts at: 0 2120 2349
Size: 2120 229
EarI CTCTTCn'nnn_
Cuts at: 0 119 131 760 1055 2349
Size: 119 12 629 295 1294
Fragments arranged by size:
1294 629 295 119 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1593 2349
Size: 1593 756
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 143 215 469 602 822 1115 2154 2184
Size: 143 72 254 133 220 293 1039 30
Cuts at: 2184 2349
Size: 165
Fragments arranged by size:
1039 293 254 220 165 143 133 72
30
EcoRI G'AATT_C
Cuts at: 0 207 1368 2349
Size: 207 1161 981
Fragments arranged by size:
1161 981 207
EcoRV GAT'ATC
Cuts at: 0 930 2349
Size: 930 1419
HaeII r_GCGC'y
Cuts at: 0 560 1881 2349
Size: 560 1321 468
Fragments arranged by size:
1321 560 468
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 232 264 636 668 1307 1339 1378 1410
Size: 232 32 372 32 639 32 39 32
Cuts at: 1410 1540 1572 1864 1896 2349
Size: 130 32 292 32 453
Fragments arranged by size:
639 453 372 292 232 130 39 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 201 2349
Size: 201 2148
HindIII A'AGCT_T
Cuts at: 0 1718 2349
Size: 1718 631
MfeI C'AATT_G
Cuts at: 0 1931 2349
Size: 1931 418
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 918 1013 2349
Size: 918 95 1336
Fragments arranged by size:
1336 918 95
MscI TGG'CCA
Cuts at: 0 2122 2349
Size: 2122 227
MslI CAynn'nnrTG
Cuts at: 0 46 2349
Size: 46 2303
MspA1I CmG'CkG
Cuts at: 0 665 1792 1980 2181 2211 2241 2349
Size: 665 1127 188 201 30 30 108
Fragments arranged by size:
1127 665 201 188 108 30 30
NdeI CA'TA_TG
Cuts at: 0 1179 2349
Size: 1179 1170
NspI r_CATG'y
Cuts at: 0 1006 2349
Size: 1006 1343
PflMI CCAn_nnn'nTGG
Cuts at: 0 1032 2349
Size: 1032 1317
PfoI T'CCnGG_A
Cuts at: 0 1010 2349
Size: 1010 1339
PmlI CAC'GTG
Cuts at: 0 1495 2349
Size: 1495 854
PvuII CAG'CTG
Cuts at: 0 1792 1980 2349
Size: 1792 188 369
Fragments arranged by size:
1792 369 188
SexAI A'CCwGG_T
Cuts at: 0 945 2349
Size: 945 1404
SmlI C'TyrA_G
Cuts at: 0 997 2349
Size: 997 1352
SspI AAT'ATT
Cuts at: 0 1947 2349
Size: 1947 402
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2026 2349
Size: 2026 323
TatI w'GTAC_w
Cuts at: 0 1647 2349
Size: 1647 702
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1457 1489 2349
Size: 1457 32 860
Fragments arranged by size:
1457 860 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 970 1743 2349
Size: 970 773 606
Fragments arranged by size:
970 773 606
XmnI GAAnn'nnTTC
Cuts at: 0 709 2349
Size: 709 1640
Enzymes that do cut:
AccI AcuI AfeI AflII AlwNI ApoI AvaI BanI
BanII BbsI BclI BglII Bme1580I BmgBI BmrI Bpu10I
BsaAI BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
Bsp1286I BsrDI BstYI Bsu36I BtgI BtsI ClaI CspCI
EaeI EarI EciI Eco57MI EcoRI EcoRV HaeII Hin4I
HincII HindIII MfeI MmeI MscI MslI MspA1I NdeI
NspI PflMI PfoI PmlI PvuII SexAI SmlI SspI
TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflIII AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BcgI BciVI BfrBI BglI
BlpI BmtI BplI BpmI BpuEI BsaI BsaHI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
BtgZI DraI DraIII DrdI EagI EcoICRI EcoNI EcoO109I
FalI FseI FspI FspAI HpaI KasI KpnI MluI
NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PmeI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI
StyI SwaI Tth111I XbaI XhoI XmaI ZraI