Restriction map for
pFN21AE0298
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# NO CUT ENZYMES ( 51 enzymes) #
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AatII AccIII AgeI AhdI Aor51HI
AscI AspEI AviII AvrII BlnI
BsaWI BseAI BsiEI BsiWI BspEI
BspHI BsrFI BssHII Bst1107I BstZ17I
Cfr10I CpoI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI FseI FspI
HpaI MluI MroI NaeI NgoMIV
NotI NruI PacI PinAI PmeI
PshAI PvuI RcaI RsrII SfiI
SgrAI SmaI SnaBI SwaI XmaCI
XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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Acc65I 7218 GGTACC G^GTACC
AclI 4504 AACGTT AA^CGTT
ApaI 3294 GGGCCC GGGCC^C
Asp718I 7218 GGTACC G^GTACC
AspI 7788 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 2216 TGGCCA TGG^CCA
BbrPI 7927 CACGTG CAC^GTG
BbvCI 4399 CCTCAGC CCTCAGC(-5/-2)
BciVI 6471 GGATAC GTATCC(6/5)
BclI 3756 TGATCA T^GATCA
BglI 7606 GCCCCTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 7012 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 7012 GCTGAGC GC^T[ACGT]AGC
BsmBI 6444 CGTCTC CGTCTC(1/5)
BspDI 4006 ATCGAT AT^CGAT
BssSI 1429 CACGAG CACGAG(-5/-1)
BstBI 5612 TTCGAA TT^CGAA
BstXI 2778 CCATGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 7012 GCTGAGC GC^T[ACGT]AGC
ClaI 4006 ATCGAT AT^CGAT
DraIII 644 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoT22I 534 ATGCAT ATGCA^T
FbaI 3756 TGATCA T^GATCA
KpnI 7218 GGTACC GGTAC^C
MfeI 4681 CAATTG C^AATTG
MluNI 2216 TGGCCA TGG^CCA
MscI 2216 TGGCCA TGG^CCA
MunI 4681 CAATTG C^AATTG
NdeI 793 CATATG CA^TATG
NheI 6631 GCTAGC G^CTAGC
NsiI 534 ATGCAT ATGCA^T
NspV 5612 TTCGAA TT^CGAA
PaeR7I 4232 CTCGAG C^TCGAG
PflFI 7788 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 7927 CACGTG CAC^GTG
PmlI 7927 CACGTG CAC^GTG
Psp1406I 4504 AACGTT AA^CGTT
SbfI 7858 CCTGCAGG CCTGCA^GG
ScaI 6679 AGTACT AGT^ACT
SfuI 5612 TTCGAA TT^CGAA
SphI 7732 GCATGC GCATG^C
Sse8387I 7858 CCTGCAGG CCTGCA^GG
Tth111I 7788 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 6652 TCTAGA T^CTAGA
XcmI 7505 CCAATACAAATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 4232 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 29 enzymes) #
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AflIII 1820 ACATGT A^C[GA][CT]GT
3651 ACATGT
AseI 4111 ATTAAT AT^TAAT
4812 ATTAAT
AsnI 4111 ATTAAT AT^TAAT
4812 ATTAAT
AvaI 3560 CTCGGG C^[CT]CG[GA]G
4232 CTCGAG
BbeI 1495 GGCGCC GGCGC^C
1525 GGCGCC
BcgI 149 GCAAAAAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4154 GCAAATCATTCG
BsaAI 1912 CACGTA [CT]AC^GT[GA]
7927 CACGTG
BsoBI 3560 CTCGGG C^[CT]CG[GA]G
4232 CTCGAG
Bsp1407I 4335 TGTACA T^GTACA
5662 TGTACA
BspLU11I 1820 ACATGT A^CATGT
3651 ACATGT
BsrBI 1275 CCGCTC CCGCTC(-3/-3)
2236 GAGCGG
BsrGI 4335 TGTACA T^GTACA
5662 TGTACA
DrdI 6031 GACTTATTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6685 GACGAGGATGTC
EaeI 2216 TGGCCA [CT]^GGCC[GA]
7407 CGGCCA
EcoRI 1860 GAATTC G^AATTC
6109 GAATTC
EcoRV 2241 GATATC GAT^ATC
4531 GATATC
KasI 1495 GGCGCC G^GCGCC
1525 GGCGCC
KspI 2305 CCGCGG CCGC^GG
8080 CCGCGG
NarI 1495 GGCGCC GG^CGCC
1525 GGCGCC
PshBI 4111 ATTAAT AT^TAAT
4812 ATTAAT
SacI 4855 GAGCTC GAGCT^C
7700 GAGCTC
SacII 2305 CCGCGG CCGC^GG
8080 CCGCGG
SalI 3324 GTCGAC G^TCGAC
5195 GTCGAC
SapI 4363 GCTCTTC GCTCTTC(1/4)
4718 GCTCTTC
SexAI 2193 ACCAGGT A^CC[AT]GGT
7775 ACCAGGT
SfoI 1495 GGCGCC GGC^GCC
1525 GGCGCC
SpeI 4408 ACTAGT A^CTAGT
4974 ACTAGT
SspBI 4335 TGTACA T^GTACA
5662 TGTACA
SspI 6195 AATATT AAT^ATT
7489 AATATT
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# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflII AluI Alw44I AlwI
AlwNI AocI ApaLI ApoI Asp700I
AspHI AvaII BamHI BanI BanII
BbsI BbvI BcnI BfaI BfrI
BglII BmyI BpmI BpuAI BsaBI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspMI BsrDI BsrI
BssKI BstAPI BstEII BstF5I BstNI
BstPI BstUI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EarI Eco81I EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII XmnI