(Circular) (Six-base) MAPSORT of: pFN21AE0298.seq Check: 6408 from: 1 to: 12859 pFN21AE0298 12859 bp With 182 enzymes: SgfI * October 20, 2009 10:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 7636 9857 9865 7636 Size: 2221 8 10630 Fragments arranged by size: 10630 2221 8 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 10669 278 Size: 53 83 186 936 9133 2468 Fragments arranged by size: 9133 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5330 6605 7201 8880 10202 1937 Size: 3393 1275 596 1679 1322 4594 Fragments arranged by size: 4594 3393 1679 1322 1275 596 Acc65I G'GTAC_C Cuts at: 9223 10180 9223 Size: 957 11902 AclI AA'CG_TT Cuts at: 1563 6510 10986 11359 1563 Size: 4947 4476 373 3063 Fragments arranged by size: 4947 4476 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2201 2385 2400 3776 4704 5117 5162 5190 Size: 569 184 15 1376 928 413 45 28 Cuts at: 5190 5606 6693 7209 7620 7875 8654 9245 9419 Size: 416 1087 516 411 255 779 591 174 Cuts at: 9419 10019 10032 10093 10922 12306 12574 1632 Size: 600 13 61 829 1384 268 1917 Fragments arranged by size: 1917 1384 1376 1087 928 829 779 600 591 569 516 416 413 411 268 255 184 174 61 45 28 15 13 AflII C'TTAA_G Cuts at: 820 1017 3721 7846 8071 820 Size: 197 2704 4125 225 5608 Fragments arranged by size: 5608 4125 2704 225 197 AflIII A'CryG_T Cuts at: 1203 3825 5656 11758 12735 1203 Size: 2622 1831 6102 977 1327 Fragments arranged by size: 6102 2622 1831 1327 977 AgeI A'CCGG_T Cuts at: 11690 11690 Size: 12859 AhdI GACnn_n'nnGTC Cuts at: 11588 11588 Size: 12859 AleI CACnn'nnGTG Cuts at: 1166 9251 9344 1166 Size: 8085 93 4681 Fragments arranged by size: 8085 4681 93 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3426 8862 9410 1184 Size: 2242 5436 548 4633 Fragments arranged by size: 5436 4633 2242 548 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3718 3750 4399 4431 9558 9590 3718 Size: 32 649 32 5127 32 6987 Fragments arranged by size: 6987 5127 649 32 32 32 AlwNI CAG_nnn'CTG Cuts at: 3413 4556 4759 5497 7795 9577 10602 12174 3413 Size: 1143 203 738 2298 1782 1025 1572 4098 Fragments arranged by size: 4098 2298 1782 1572 1143 1025 738 203 ApaI G_GGCC'C Cuts at: 5303 5303 Size: 12859 ApaLI G'TGCA_C Cuts at: 3551 3771 10103 10916 12072 3551 Size: 220 6332 813 1156 4338 Fragments arranged by size: 6332 4338 1156 813 220 ApoI r'AATT_y Cuts at: 1493 2022 2468 3865 4921 4940 6631 7054 7112 Size: 529 446 1397 1056 19 1691 423 58 Cuts at: 7112 7150 7538 7574 8114 8164 8937 10168 10394 Size: 38 388 36 540 50 773 1231 226 Cuts at: 10394 10546 12655 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1691 1397 1231 1056 773 540 529 446 423 388 226 152 58 50 38 36 19 AseI AT'TA_AT Cuts at: 160 1032 6117 6818 11413 160 Size: 872 5085 701 4595 1606 Fragments arranged by size: 5085 4595 1606 872 701 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 12859 AvaI C'yCGr_G Cuts at: 1870 1943 5565 6237 10184 1870 Size: 73 3622 672 3947 4545 Fragments arranged by size: 4545 3947 3622 672 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 9430 9463 9430 Size: 33 12826 BamHI G'GATC_C Cuts at: 3748 5317 9213 10189 10556 3748 Size: 1569 3896 976 367 6051 Fragments arranged by size: 6051 3896 1569 976 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3500 3530 6892 9223 10180 Size: 325 217 627 1713 30 3362 2331 957 Cuts at: 10180 11635 618 Size: 1455 1842 Fragments arranged by size: 3362 2331 1842 1713 1455 957 627 325 217 30 BanII G_rGCy'C Cuts at: 1391 2648 5303 6864 7425 8987 9709 10178 1391 Size: 1257 2655 1561 561 1562 722 469 4072 Fragments arranged by size: 4072 2655 1562 1561 1257 722 561 469 BbeI G_GCGC'C Cuts at: 3504 3534 3504 Size: 30 12829 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2766 3480 4719 6642 8583 8819 928 Size: 971 867 714 1239 1923 1941 236 4968 Fragments arranged by size: 4968 1941 1923 1239 971 867 714 236 BbvCI CC'TCA_GC Cuts at: 6405 6405 Size: 12859 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2143 2177 6148 6182 11050 Size: 34 50 34 122 34 3971 34 4868 Cuts at: 11050 11084 1903 Size: 34 3678 Fragments arranged by size: 4868 3971 3678 122 50 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 8470 10751 11961 8470 Size: 2281 1210 9368 Fragments arranged by size: 9368 2281 1210 BclI T'GATC_A Cuts at: 1548 5761 1548 Size: 4213 8646 BfrBI ATG'CAT Cuts at: 2541 2541 Size: 12859 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 9617 11470 136 Size: 107 122 71 631 605 7945 1853 1525 Fragments arranged by size: 7945 1853 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3029 3653 6991 9639 12854 3029 Size: 624 3338 2648 3215 3034 Fragments arranged by size: 3338 3215 3034 2648 624 BlpI GC'TnA_GC Cuts at: 9018 10276 9018 Size: 1258 11601 Bme1580I G_kGCm'C Cuts at: 1790 3555 3775 4668 5013 5303 9258 10107 10920 Size: 1765 220 893 345 290 3955 849 813 Cuts at: 10920 12076 1790 Size: 1156 2573 Fragments arranged by size: 3955 2573 1765 1156 893 849 813 345 290 220 BmgBI CAC'GTC Cuts at: 1318 7333 9984 10117 1318 Size: 6015 2651 133 4060 Fragments arranged by size: 6015 4060 2651 133 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3398 5202 5474 Size: 416 53 83 399 24 1981 1804 272 Cuts at: 5474 6261 6369 10590 11548 442 Size: 787 108 4221 958 1753 Fragments arranged by size: 4221 1981 1804 1753 958 787 416 399 272 108 83 53 24 BmtI G_CTAG'C Cuts at: 1056 8640 1056 Size: 7584 5275 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2229 2229 Size: 12859 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3312 3829 5617 5896 6481 7680 9099 9390 Size: 2073 517 1788 279 585 1199 1419 291 Cuts at: 9390 9620 9693 10035 11519 1239 Size: 230 73 342 1484 2579 Fragments arranged by size: 2579 2073 1788 1484 1419 1199 585 517 342 291 279 230 73 Bpu10I CC'TnA_GC Cuts at: 4995 5304 5429 5457 6405 6863 8783 4995 Size: 309 125 28 948 458 1920 9071 Fragments arranged by size: 9071 1920 948 458 309 125 28 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2482 4256 5659 9063 10984 11849 12147 2482 Size: 1774 1403 3404 1921 865 298 3194 Fragments arranged by size: 3404 3194 1921 1774 1403 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5526 5580 5733 6148 7330 9638 11522 Size: 617 4027 54 153 415 1182 2308 1884 Cuts at: 11522 882 Size: 2219 Fragments arranged by size: 4027 2308 2219 1884 1182 617 415 153 54 BsaAI yAC'GTr Cuts at: 493 1204 3919 9934 10650 493 Size: 711 2715 6015 716 2702 Fragments arranged by size: 6015 2715 2702 716 711 BsaBI GATnn'nnATC Cuts at: 2443 5537 6327 12853 2443 Size: 3094 790 6526 2449 Fragments arranged by size: 6526 3094 2449 790 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3501 3531 5078 8448 Size: 53 83 186 936 1968 30 1547 3370 Cuts at: 8448 10666 11048 11683 275 Size: 2218 382 635 1451 Fragments arranged by size: 3370 2218 1968 1547 1451 936 635 382 186 83 53 30 BsaWI w'CCGG_w Cuts at: 11292 11690 11964 12111 12522 11292 Size: 398 274 147 411 11629 Fragments arranged by size: 11629 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4399 4429 5408 5438 5908 Size: 30 165 30 2996 30 979 30 470 Cuts at: 5908 5938 7950 7980 8806 8836 9784 9814 10033 Size: 30 2012 30 826 30 948 30 219 Cuts at: 10033 10063 1178 Size: 30 3974 Fragments arranged by size: 3974 2996 2012 979 948 826 470 219 165 30 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3033 3506 4253 4563 4566 4825 5628 9792 Size: 1847 473 747 310 3 259 803 4164 Cuts at: 9792 1186 Size: 4253 Fragments arranged by size: 4253 4164 1847 803 747 473 310 259 3 BseYI C'CCAG_C Cuts at: 3936 4094 5051 5793 7252 7788 9168 12062 12558 Size: 158 957 742 1459 536 1380 2894 496 Cuts at: 12558 3936 Size: 4237 Fragments arranged by size: 4237 2894 1459 1380 957 742 536 496 158 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2676 6905 8341 9296 1163 Size: 573 940 4229 1436 955 4726 Fragments arranged by size: 4726 4229 1436 955 940 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 10309 11070 12098 1540 Size: 275 186 8308 761 1028 2301 Fragments arranged by size: 8308 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2630 3555 3775 5001 6584 6864 8789 9024 9709 Size: 925 220 1226 1583 280 1925 235 685 Cuts at: 9709 10107 10920 11005 12076 2630 Size: 398 813 85 1071 3413 Fragments arranged by size: 3413 1925 1583 1226 1071 925 813 685 398 280 235 220 85 BsmI GAATG_Cn' Cuts at: 2543 2588 2965 5194 6388 6739 7093 9163 9562 Size: 45 377 2229 1194 351 354 2070 399 Cuts at: 9562 10370 10463 2543 Size: 808 93 4939 Fragments arranged by size: 4939 2229 2070 1194 808 399 377 354 351 93 45 BsmBI CGTCTCn'nnnn_ Cuts at: 8455 8455 Size: 12859 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2630 2648 3555 3775 4668 5001 5013 Size: 399 840 18 907 220 893 333 12 Cuts at: 5013 5303 6584 6864 7425 8789 8987 9024 9258 Size: 290 1281 280 561 1364 198 37 234 Cuts at: 9258 9709 10107 10178 10920 11005 12076 1391 Size: 451 398 71 742 85 1071 2174 Fragments arranged by size: 2174 1364 1281 1071 907 893 840 742 561 451 399 398 333 290 280 234 220 198 85 71 37 18 12 BspEI T'CCGG_A Cuts at: 12522 12522 Size: 12859 BspHI T'CATG_A Cuts at: 10748 12478 10748 Size: 1730 11129 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4564 7636 9235 9562 9857 9865 10214 844 Size: 3720 3072 1599 327 295 8 349 3489 Fragments arranged by size: 3720 3489 3072 1599 349 327 295 8 BsrBI CCG'CTC Cuts at: 1394 1823 3282 4243 10746 1394 Size: 429 1459 961 6503 3507 Fragments arranged by size: 6503 3507 1459 961 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2616 2838 4187 7269 9653 11354 11528 Size: 1183 1368 222 1349 3082 2384 1701 174 Cuts at: 11528 65 Size: 1396 Fragments arranged by size: 3082 2384 1701 1396 1368 1349 1183 222 174 BsrFI r'CCGG_y Cuts at: 1700 1810 11503 11690 1700 Size: 110 9693 187 2869 Fragments arranged by size: 9693 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 6340 7667 96 Size: 6244 1327 5288 Fragments arranged by size: 6244 5288 1327 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 12859 BssSI C'ACGA_G Cuts at: 3434 10919 11931 3434 Size: 7485 1012 4362 Fragments arranged by size: 7485 4362 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3550 4556 6745 6847 6892 9738 794 Size: 2756 1006 2189 102 45 2846 3915 Fragments arranged by size: 3915 2846 2756 2189 1006 102 45 BstBI TT'CG_AA Cuts at: 7618 11663 7618 Size: 4045 8814 BstEII G'GTnAC_C Cuts at: 1184 4089 9810 9869 1184 Size: 2905 5721 59 4174 Fragments arranged by size: 5721 4174 2905 59 BstXI CCAn_nnnn'nTGG Cuts at: 4790 4790 Size: 12859 BstYI r'GATC_y Cuts at: 1972 2444 3029 3653 3748 5317 6472 6766 6991 Size: 472 585 624 95 1569 1155 294 225 Cuts at: 6991 9213 9639 10189 10556 10941 10958 12410 12496 Size: 2222 426 550 367 385 17 1452 86 Cuts at: 12496 12508 12854 1972 Size: 12 346 1977 Fragments arranged by size: 2222 1977 1569 1452 1155 624 585 550 472 426 385 367 346 294 225 95 86 17 12 Bsu36I CC'TnA_GG Cuts at: 9615 9804 10030 9615 Size: 189 226 12444 Fragments arranged by size: 12444 226 189 BtgI C'CryG_G Cuts at: 513 1065 3534 3744 4310 5313 7275 10085 513 Size: 552 2469 210 566 1003 1962 2810 3287 Fragments arranged by size: 3287 2810 2469 1962 1003 566 552 210 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 10140 487 Size: 683 56 8914 3206 Fragments arranged by size: 8914 3206 683 56 BtsI GCAGTG_nn' Cuts at: 2583 2997 6393 8353 9394 10375 11169 11189 2583 Size: 414 3396 1960 1041 981 794 20 4253 Fragments arranged by size: 4253 3396 1960 1041 981 794 414 20 ClaI AT'CG_AT Cuts at: 1554 6012 1554 Size: 4458 8401 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 7923 7936 7958 7971 8092 8127 529 Size: 35 7359 13 22 13 121 35 5261 Fragments arranged by size: 7359 5261 121 35 35 22 13 13 DraI TTT'AAA Cuts at: 3270 4369 4392 6309 10163 3270 Size: 1099 23 1917 3854 5966 Fragments arranged by size: 5966 3854 1917 1099 23 DraIII CAC_nnn'GTG Cuts at: 2654 2654 Size: 12859 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8042 8696 11866 809 Size: 507 6726 654 3170 1802 Fragments arranged by size: 6726 3170 1802 654 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4221 9412 10306 Size: 54 1425 50 275 18 2391 5191 894 Cuts at: 10306 11195 12824 8 Size: 889 1629 43 Fragments arranged by size: 5191 2391 1629 1425 894 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 10306 1537 Size: 275 8494 4090 Fragments arranged by size: 8494 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2220 2238 2551 4723 6375 6730 8223 8251 Size: 867 18 313 2172 1652 355 1493 28 Cuts at: 8251 10789 1353 Size: 2538 3423 Fragments arranged by size: 3423 2538 2172 1652 1493 867 355 313 28 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 8333 11446 11820 11966 387 Size: 1064 138 6744 3113 374 146 1280 Fragments arranged by size: 6744 3113 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 6862 9707 6862 Size: 2845 10014 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2201 2385 2400 3312 3776 3829 4704 Size: 393 569 184 15 912 464 53 875 Cuts at: 4704 5117 5162 5190 5606 5617 5896 6481 6693 Size: 413 45 28 416 11 279 585 212 Cuts at: 6693 7209 7620 7680 7875 8654 9099 9245 9390 Size: 516 411 60 195 779 445 146 145 Cuts at: 9390 9419 9620 9693 10019 10032 10035 10093 10922 Size: 29 201 73 326 13 3 58 829 Cuts at: 10922 11519 12306 12574 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 912 875 829 787 779 597 585 569 516 464 445 416 413 411 393 326 279 268 212 201 195 184 146 145 73 60 58 53 45 29 28 15 13 11 3 EcoO109I rG'GnC_Cy Cuts at: 3787 4649 5055 5299 5300 6401 6867 8674 8892 Size: 862 406 244 1 1101 466 1807 218 Cuts at: 8892 3787 Size: 7754 Fragments arranged by size: 7754 1807 1101 862 466 406 244 218 1 EcoRI G'AATT_C Cuts at: 3865 8114 10168 10546 3865 Size: 4249 2054 378 6178 Fragments arranged by size: 6178 4249 2054 378 EcoRV GAT'ATC Cuts at: 4248 6538 4248 Size: 2290 10569 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2422 4493 5577 7976 8053 2422 Size: 2071 1084 2399 77 7228 Fragments arranged by size: 7228 2399 2071 1084 77 FspI TGC'GCA Cuts at: 11365 11365 Size: 12859 HaeII r_GCGC'y Cuts at: 1678 2457 3504 3534 4623 7920 8762 12006 1678 Size: 779 1047 30 1089 3297 842 3244 2531 Fragments arranged by size: 3297 3244 2531 1089 1047 842 779 30 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4232 4264 4663 4695 5302 Size: 32 135 32 2660 32 399 32 607 Cuts at: 5302 5334 6815 6847 6976 7008 7505 7537 8806 Size: 32 1481 32 129 32 497 32 1269 Cuts at: 8806 8838 9784 9816 10033 10065 11500 11532 11574 Size: 32 946 32 217 32 1435 32 42 Cuts at: 11574 11606 1373 Size: 32 2626 Fragments arranged by size: 2660 2626 1481 1435 1269 946 607 497 399 217 135 129 42 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4155 5331 7202 7745 10203 10449 Size: 988 281 2217 1176 1871 543 2458 246 Cuts at: 10449 669 Size: 3079 Fragments arranged by size: 3079 2458 2217 1871 1176 988 543 281 246 HindIII A'AGCT_T Cuts at: 748 3598 4253 4272 748 Size: 2850 655 19 9335 Fragments arranged by size: 9335 2850 655 19 HpaI GTT'AAC Cuts at: 10449 10449 Size: 12859 KasI G'GCGC_C Cuts at: 3500 3530 3500 Size: 30 12829 KpnI G_GTAC'C Cuts at: 9227 10184 9227 Size: 957 11902 MfeI C'AATT_G Cuts at: 6686 10458 6686 Size: 3772 9087 MluI A'CGCG_T Cuts at: 12735 12735 Size: 12859 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2910 3831 6754 7422 9544 11973 12157 1305 Size: 1605 921 2923 668 2122 2429 184 2007 Fragments arranged by size: 2923 2429 2122 2007 1605 921 668 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4223 10 Size: 54 1425 343 2391 8646 Fragments arranged by size: 8646 2391 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2862 4805 7086 7274 8337 8771 9251 Size: 648 1696 1943 2281 188 1063 434 480 Cuts at: 9251 9344 10817 11176 11335 518 Size: 93 1473 359 159 2042 Fragments arranged by size: 2281 2042 1943 1696 1473 1063 648 480 434 359 188 159 93 MspA1I CmG'CkG Cuts at: 1606 4312 6050 7511 7795 9017 9041 9826 10087 Size: 2706 1738 1461 284 1222 24 785 261 Cuts at: 10087 10275 10952 12100 12345 1606 Size: 188 677 1148 245 2120 Fragments arranged by size: 2706 2120 1738 1461 1222 1148 785 677 284 261 245 188 24 NarI GG'CG_CC Cuts at: 3501 3531 3501 Size: 30 12829 NcoI C'CATG_G Cuts at: 513 1065 3534 3744 5313 7275 513 Size: 552 2469 210 1569 1962 6097 Fragments arranged by size: 6097 2469 1962 1569 552 210 NdeI CA'TA_TG Cuts at: 387 2799 387 Size: 2412 10447 NheI G'CTAG_C Cuts at: 1052 8636 1052 Size: 7584 5275 NotI GC'GGCC_GC Cuts at: 10306 10306 Size: 12859 NsiI A_TGCA'T Cuts at: 2543 2543 Size: 12859 NspI r_CATG'y Cuts at: 1132 1229 3249 3829 5660 6260 8311 9741 10217 Size: 97 2020 580 1831 600 2051 1430 476 Cuts at: 10217 11762 1132 Size: 1545 2229 Fragments arranged by size: 2229 2051 2020 1831 1545 1430 600 580 476 97 PasI CC'CwG_GG Cuts at: 1348 3832 8754 1348 Size: 2484 4922 5453 Fragments arranged by size: 5453 4922 2484 PciI A'CATG_T Cuts at: 3825 5656 11758 3825 Size: 1831 6102 4926 Fragments arranged by size: 6102 4926 1831 PflMI CCAn_nnn'nTGG Cuts at: 1108 3058 3413 7795 1108 Size: 1950 355 4382 6172 Fragments arranged by size: 6172 4382 1950 355 PfoI T'CCnGG_A Cuts at: 5490 5912 6073 5490 Size: 422 161 12276 Fragments arranged by size: 12276 422 161 PmeI GTTT'AAAC Cuts at: 10163 10163 Size: 12859 PmlI CAC'GTG Cuts at: 9934 9934 Size: 12859 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4399 4431 10928 10960 12441 12473 4399 Size: 32 6497 32 1481 32 4785 Fragments arranged by size: 6497 4785 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3787 4649 5055 8674 8892 3787 Size: 862 406 3619 218 7754 Fragments arranged by size: 7754 3619 862 406 218 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 12859 PsiI TTA'TAA Cuts at: 2982 9192 10429 2982 Size: 6210 1237 5412 Fragments arranged by size: 6210 5412 1237 PspOMI G'GGCC_C Cuts at: 5299 5299 Size: 12859 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 9304 9336 9304 Size: 32 12827 PstI C_TGCA'G Cuts at: 830 2418 3546 3661 4138 5025 5385 6480 6927 Size: 1588 1128 115 477 887 360 1095 447 Cuts at: 6927 9016 9573 9868 10211 830 Size: 2089 557 295 343 3478 Fragments arranged by size: 3478 2089 1588 1128 1095 887 557 477 447 360 343 295 115 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 12859 PvuII CAG'CTG Cuts at: 6050 7511 7795 9017 6050 Size: 1461 284 1222 9892 Fragments arranged by size: 9892 1461 1222 284 SacI G_AGCT'C Cuts at: 6864 9709 6864 Size: 2845 10014 SacII CC_GC'GG Cuts at: 4313 10088 4313 Size: 5775 7084 SalI G'TCGA_C Cuts at: 1936 5329 7200 10201 1936 Size: 3393 1871 3001 4594 Fragments arranged by size: 4594 3393 3001 1871 SapI GCTCTTCn'nnn_ Cuts at: 6375 6730 6375 Size: 355 12504 SbfI CC_TGCA'GG Cuts at: 9868 10211 9868 Size: 343 12516 ScaI AGT'ACT Cuts at: 8686 11107 12819 8686 Size: 2421 1712 8726 Fragments arranged by size: 8726 2421 1712 SexAI A'CCwGG_T Cuts at: 4198 9780 4198 Size: 5582 7277 SfcI C'TryA_G Cuts at: 826 1045 2414 3003 3542 3644 3657 4134 5021 Size: 219 1369 589 539 102 13 477 887 Cuts at: 5021 5041 5381 6476 6923 7416 8877 9012 9104 Size: 20 340 1095 447 493 1461 135 92 Cuts at: 9104 9569 9765 9816 9864 10207 11342 12023 12214 Size: 465 196 51 48 343 1135 681 191 Cuts at: 12214 826 Size: 1471 Fragments arranged by size: 1471 1461 1369 1135 1095 887 681 589 539 493 477 465 447 343 340 219 196 191 135 102 92 51 48 20 13 SfoI GGC'GCC Cuts at: 3502 3532 3502 Size: 30 12829 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 12859 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 12859 SmaI CCC'GGG Cuts at: 1872 10186 1872 Size: 8314 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2497 3721 4235 5674 6237 7846 Size: 197 926 554 1224 514 1439 563 1609 Cuts at: 7846 8071 9078 10963 11864 12126 820 Size: 225 1007 1885 901 262 1553 Fragments arranged by size: 1885 1609 1553 1439 1224 1007 926 901 563 554 514 262 225 197 SnaBI TAC'GTA Cuts at: 493 10650 493 Size: 10157 2702 SpeI A'CTAG_T Cuts at: 152 6413 6979 152 Size: 6261 566 6032 Fragments arranged by size: 6261 6032 566 SphI G_CATG'C Cuts at: 1132 9741 10217 1132 Size: 8609 476 3774 Fragments arranged by size: 8609 3774 476 SspI AAT'ATT Cuts at: 5 52 8202 9496 10783 5 Size: 47 8150 1294 1287 2081 Fragments arranged by size: 8150 2081 1294 1287 47 StuI AGG'CCT Cuts at: 1517 3009 5175 9432 9504 1517 Size: 1492 2166 4257 72 4872 Fragments arranged by size: 4872 4257 2166 1492 72 StyI C'CwwG_G Cuts at: 513 1065 3534 3744 4774 5313 6871 7275 8415 Size: 552 2469 210 1030 539 1558 404 1140 Cuts at: 8415 10298 513 Size: 1883 3074 Fragments arranged by size: 3074 2469 1883 1558 1140 1030 552 539 404 210 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5854 6360 7802 9809 10886 10903 11056 Size: 1255 4274 506 1442 2007 1077 17 153 Cuts at: 11056 11241 11686 325 Size: 185 445 1498 Fragments arranged by size: 4274 2007 1498 1442 1255 1077 506 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 6340 6416 7412 Size: 274 80 33 51 1443 4363 76 996 Cuts at: 7412 7667 8684 11105 12817 96 Size: 255 1017 2421 1712 138 Fragments arranged by size: 4363 2421 1712 1443 1017 996 274 255 138 80 76 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3350 3382 5408 5440 6867 6899 7950 7982 8396 Size: 32 2026 32 1427 32 1051 32 414 Cuts at: 8396 8428 12819 12851 3350 Size: 32 4391 32 3358 Fragments arranged by size: 4391 3358 2026 1427 1051 414 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 9796 1316 Size: 8480 4379 XbaI T'CTAG_A Cuts at: 8657 10195 8657 Size: 1538 11321 XcmI CCAnnnn_n'nnnnTGG Cuts at: 9517 9517 Size: 12859 XhoI C'TCGA_G Cuts at: 1943 6237 1943 Size: 4294 8565 XmaI C'CCGG_G Cuts at: 1870 10184 1870 Size: 8314 4545 XmnI GAAnn'nnTTC Cuts at: 4414 4522 5130 5175 8937 10988 4414 Size: 108 608 45 3762 2051 6285 Fragments arranged by size: 6285 3762 2051 608 108 45 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 10667 276 Size: 53 83 186 936 9133 2468 Fragments arranged by size: 9133 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BsiWI BstZ17I EcoNI FseI FspAI NaeI NgoMIV NruI PacI PspXI RsrII SanDI SfiI SrfI SwaI