Restriction map for
pFN21AE0298
(Linear) (Six-base) MAPSORT of: KIEE0298.seq Check: 4941 from: 1 to: 8154
KIEE0298.orf
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 5631 7852 7860 8154
Size: 5631 2221 8 294
Fragments arranged by size:
5631 2221 294 8
AccI GT'mk_AC
Cuts at: 0 3325 4600 5196 6875 8154
Size: 3325 1275 596 1679 1279
Fragments arranged by size:
3325 1679 1279 1275 596
Acc65I G'GTAC_C
Cuts at: 0 7218 8154
Size: 7218 936
AclI AA'CG_TT
Cuts at: 0 4505 8154
Size: 4505 3649
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 380 395 1771 2699 3112 3157 3185
Size: 196 184 15 1376 928 413 45 28
Cuts at: 3185 3601 4688 5204 5615 5870 6649 7240 7414
Size: 416 1087 516 411 255 779 591 174
Cuts at: 7414 8014 8027 8088 8154
Size: 600 13 61 66
Fragments arranged by size:
1376 1087 928 779 600 591 516 416
413 411 255 196 184 174 66 61
45 28 15 13
AflII C'TTAA_G
Cuts at: 0 1716 5841 6066 8154
Size: 1716 4125 225 2088
Fragments arranged by size:
4125 2088 1716 225
AflIII A'CryG_T
Cuts at: 0 1820 3651 8154
Size: 1820 1831 4503
Fragments arranged by size:
4503 1831 1820
AleI CACnn'nnGTG
Cuts at: 0 7246 7339 8154
Size: 7246 93 815
Fragments arranged by size:
7246 815 93
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1421 6857 7405 8154
Size: 1421 5436 548 749
Fragments arranged by size:
5436 1421 749 548
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1713 1745 2394 2426 7553 7585 8154
Size: 1713 32 649 32 5127 32 569
Fragments arranged by size:
5127 1713 649 569 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1408 2551 2754 3492 5790 7572 8154
Size: 1408 1143 203 738 2298 1782 582
Fragments arranged by size:
2298 1782 1408 1143 738 582 203
ApaI G_GGCC'C
Cuts at: 0 3298 8154
Size: 3298 4856
ApaLI G'TGCA_C
Cuts at: 0 1546 1766 8098 8154
Size: 1546 220 6332 56
Fragments arranged by size:
6332 1546 220 56
ApoI r'AATT_y
Cuts at: 0 17 463 1860 2916 2935 4626 5049 5107
Size: 17 446 1397 1056 19 1691 423 58
Cuts at: 5107 5145 5533 5569 6109 6159 6932 8154
Size: 38 388 36 540 50 773 1222
Fragments arranged by size:
1691 1397 1222 1056 773 540 446 423
388 58 50 38 36 19 17
AseI AT'TA_AT
Cuts at: 0 4112 4813 8154
Size: 4112 701 3341
Fragments arranged by size:
4112 3341 701
AvaI C'yCGr_G
Cuts at: 0 3560 4232 8154
Size: 3560 672 3922
Fragments arranged by size:
3922 3560 672
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 7425 7458 8154
Size: 7425 33 696
Fragments arranged by size:
7425 696 33
BamHI G'GATC_C
Cuts at: 0 1743 3312 7208 8154
Size: 1743 1569 3896 946
Fragments arranged by size:
3896 1743 1569 946
BanI G'GyrC_C
Cuts at: 0 1495 1525 4887 7218 8154
Size: 1495 30 3362 2331 936
Fragments arranged by size:
3362 2331 1495 936 30
BanII G_rGCy'C
Cuts at: 0 643 3298 4859 5420 6982 7704 8154
Size: 643 2655 1561 561 1562 722 450
Fragments arranged by size:
2655 1562 1561 722 643 561 450
BbeI G_GCGC'C
Cuts at: 0 1499 1529 8154
Size: 1499 30 6625
Fragments arranged by size:
6625 1499 30
BbsI GAAGACnn'nnnn_
Cuts at: 0 761 1475 2714 4637 6578 6814 8154
Size: 761 714 1239 1923 1941 236 1340
Fragments arranged by size:
1941 1923 1340 1239 761 714 236
BbvCI CC'TCA_GC
Cuts at: 0 4400 8154
Size: 4400 3754
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 138 172 4143 4177 8154
Size: 138 34 3971 34 3977
Fragments arranged by size:
3977 3971 138 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 6465 8154
Size: 6465 1689
BclI T'GATC_A
Cuts at: 0 3756 8154
Size: 3756 4398
BfrBI ATG'CAT
Cuts at: 0 536 8154
Size: 536 7618
BglI GCCn_nnn'nGGC
Cuts at: 0 7612 8154
Size: 7612 542
BglII A'GATC_T
Cuts at: 0 1024 1648 4986 7634 8154
Size: 1024 624 3338 2648 520
Fragments arranged by size:
3338 2648 1024 624 520
BlpI GC'TnA_GC
Cuts at: 0 7013 8154
Size: 7013 1141
Bme1580I G_kGCm'C
Cuts at: 0 1550 1770 2663 3008 3298 7253 8102 8154
Size: 1550 220 893 345 290 3955 849 52
Fragments arranged by size:
3955 1550 893 849 345 290 220 52
BmgBI CAC'GTC
Cuts at: 0 5328 7979 8112 8154
Size: 5328 2651 133 42
Fragments arranged by size:
5328 2651 133 42
BmrI ACTGGGnnnn_n'
Cuts at: 0 1393 3197 3469 4256 4364 8154
Size: 1393 1804 272 787 108 3790
Fragments arranged by size:
3790 1804 1393 787 272 108
BmtI G_CTAG'C
Cuts at: 0 6635 8154
Size: 6635 1519
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 224 8154
Size: 224 7930
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1307 1824 3612 3891 4476 5675 7094 7385
Size: 1307 517 1788 279 585 1199 1419 291
Cuts at: 7385 7615 7688 8030 8154
Size: 230 73 342 124
Fragments arranged by size:
1788 1419 1307 1199 585 517 342 291
279 230 124 73
Bpu10I CC'TnA_GC
Cuts at: 0 2990 3299 3424 3452 4400 4858 6778 8154
Size: 2990 309 125 28 948 458 1920 1376
Fragments arranged by size:
2990 1920 1376 948 458 309 125 28
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 477 2251 3654 7058 8154
Size: 477 1774 1403 3404 1096
Fragments arranged by size:
3404 1774 1403 1096 477
BsaI GGTCTCn'nnnn_
Cuts at: 0 3521 3575 3728 4143 5325 7633 8154
Size: 3521 54 153 415 1182 2308 521
Fragments arranged by size:
3521 2308 1182 521 415 153 54
BsaAI yAC'GTr
Cuts at: 0 1914 7929 8154
Size: 1914 6015 225
Fragments arranged by size:
6015 1914 225
BsaBI GATnn'nnATC
Cuts at: 0 438 3532 4322 8154
Size: 438 3094 790 3832
Fragments arranged by size:
3832 3094 790 438
BsaHI Gr'CG_yC
Cuts at: 0 1496 1526 3073 6443 8154
Size: 1496 30 1547 3370 1711
Fragments arranged by size:
3370 1711 1547 1496 30
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2394 2424 3403 3433 3903 3933 5945 5975
Size: 2394 30 979 30 470 30 2012 30
Cuts at: 5975 6801 6831 7779 7809 8028 8058 8154
Size: 826 30 948 30 219 30 96
Fragments arranged by size:
2394 2012 979 948 826 470 219 96
30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1028 1501 2248 2558 2561 2820 3623 7787
Size: 1028 473 747 310 3 259 803 4164
Cuts at: 7787 8154
Size: 367
Fragments arranged by size:
4164 1028 803 747 473 367 310 259
3
BseYI C'CCAG_C
Cuts at: 0 1931 2089 3046 3788 5247 5783 7163 8154
Size: 1931 158 957 742 1459 536 1380 991
Fragments arranged by size:
1931 1459 1380 991 957 742 536 158
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 671 4900 6336 7291 8154
Size: 671 4229 1436 955 863
Fragments arranged by size:
4229 1436 955 863 671
BsiHKAI G_wGCw'C
Cuts at: 0 625 1550 1770 2996 4579 4859 6784 7019
Size: 625 925 220 1226 1583 280 1925 235
Cuts at: 7019 7704 8102 8154
Size: 685 398 52
Fragments arranged by size:
1925 1583 1226 925 685 625 398 280
235 220 52
BsmI GAATG_Cn'
Cuts at: 0 538 583 960 3189 4383 4734 5088 7158
Size: 538 45 377 2229 1194 351 354 2070
Cuts at: 7158 7557 8154
Size: 399 597
Fragments arranged by size:
2229 2070 1194 597 538 399 377 354
351 45
BsmBI CGTCTCn'nnnn_
Cuts at: 0 6450 8154
Size: 6450 1704
Bsp1286I G_dGCh'C
Cuts at: 0 625 643 1550 1770 2663 2996 3008 3298
Size: 625 18 907 220 893 333 12 290
Cuts at: 3298 4579 4859 5420 6784 6982 7019 7253 7704
Size: 1281 280 561 1364 198 37 234 451
Cuts at: 7704 8102 8154
Size: 398 52
Fragments arranged by size:
1364 1281 907 893 625 561 451 398
333 290 280 234 220 198 52 37
18 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2559 5631 7230 7557 7852 7860 8154
Size: 2559 3072 1599 327 295 8 294
Fragments arranged by size:
3072 2559 1599 327 295 294 8
BsrBI CCG'CTC
Cuts at: 0 1277 2238 8154
Size: 1277 961 5916
Fragments arranged by size:
5916 1277 961
BsrDI GCAATG_nn'
Cuts at: 0 611 833 2182 5264 7648 8154
Size: 611 222 1349 3082 2384 506
Fragments arranged by size:
3082 2384 1349 611 506 222
BsrGI T'GTAC_A
Cuts at: 0 4335 5662 8154
Size: 4335 1327 2492
Fragments arranged by size:
4335 2492 1327
BssSI C'ACGA_G
Cuts at: 0 1429 8154
Size: 1429 6725
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1545 2551 4740 4842 4887 7733 8154
Size: 1545 1006 2189 102 45 2846 421
Fragments arranged by size:
2846 2189 1545 1006 421 102 45
BstBI TT'CG_AA
Cuts at: 0 5613 8154
Size: 5613 2541
BstEII G'GTnAC_C
Cuts at: 0 2084 7805 7864 8154
Size: 2084 5721 59 290
Fragments arranged by size:
5721 2084 290 59
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2785 8154
Size: 2785 5369
BstYI r'GATC_y
Cuts at: 0 439 1024 1648 1743 3312 4467 4761 4986
Size: 439 585 624 95 1569 1155 294 225
Cuts at: 4986 7208 7634 8154
Size: 2222 426 520
Fragments arranged by size:
2222 1569 1155 624 585 520 439 426
294 225 95
Bsu36I CC'TnA_GG
Cuts at: 0 7610 7799 8025 8154
Size: 7610 189 226 129
Fragments arranged by size:
7610 226 189 129
BtgI C'CryG_G
Cuts at: 0 1529 1739 2305 3308 5270 8080 8154
Size: 1529 210 566 1003 1962 2810 74
Fragments arranged by size:
2810 1962 1529 1003 566 210 74
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 8135 8154
Size: 8135 19
BtsI GCAGTG_nn'
Cuts at: 0 578 992 4388 6348 7389 8154
Size: 578 414 3396 1960 1041 765
Fragments arranged by size:
3396 1960 1041 765 578 414
ClaI AT'CG_AT
Cuts at: 0 4007 8154
Size: 4007 4147
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 5918 5931 5953 5966 6087 6122 8154
Size: 5918 13 22 13 121 35 2032
Fragments arranged by size:
5918 2032 121 35 22 13 13
DraI TTT'AAA
Cuts at: 0 1265 2364 2387 4304 8154
Size: 1265 1099 23 1917 3850
Fragments arranged by size:
3850 1917 1265 1099 23
DraIII CAC_nnn'GTG
Cuts at: 0 649 8154
Size: 649 7505
DrdI GACnn_nn'nnGTC
Cuts at: 0 6037 6691 8154
Size: 6037 654 1463
Fragments arranged by size:
6037 1463 654
EaeI y'GGCC_r
Cuts at: 0 2216 7407 8154
Size: 2216 5191 747
Fragments arranged by size:
5191 2216 747
EarI CTCTTCn'nnn_
Cuts at: 0 215 233 546 2718 4370 4725 6218 6246
Size: 215 18 313 2172 1652 355 1493 28
Cuts at: 6246 8154
Size: 1908
Fragments arranged by size:
2172 1908 1652 1493 355 313 215 28
18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 6328 8154
Size: 6328 1826
EcoICRI GAG'CTC
Cuts at: 0 4857 7702 8154
Size: 4857 2845 452
Fragments arranged by size:
4857 2845 452
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 380 395 1307 1771 1824 2699 3112
Size: 196 184 15 912 464 53 875 413
Cuts at: 3112 3157 3185 3601 3612 3891 4476 4688 5204
Size: 45 28 416 11 279 585 212 516
Cuts at: 5204 5615 5675 5870 6649 7094 7240 7385 7414
Size: 411 60 195 779 445 146 145 29
Cuts at: 7414 7615 7688 8014 8027 8030 8088 8154
Size: 201 73 326 13 3 58 66
Fragments arranged by size:
912 875 779 585 516 464 445 416
413 411 326 279 212 201 196 195
184 146 145 73 66 60 58 53
45 29 28 15 13 11 3
EcoO109I rG'GnC_Cy
Cuts at: 0 1782 2644 3050 3294 3295 4396 4862 6669
Size: 1782 862 406 244 1 1101 466 1807
Cuts at: 6669 6887 8154
Size: 218 1267
Fragments arranged by size:
1807 1782 1267 1101 862 466 406 244
218 1
EcoRI G'AATT_C
Cuts at: 0 1860 6109 8154
Size: 1860 4249 2045
Fragments arranged by size:
4249 2045 1860
EcoRV GAT'ATC
Cuts at: 0 2243 4533 8154
Size: 2243 2290 3621
Fragments arranged by size:
3621 2290 2243
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 417 2488 3572 5971 6048 8154
Size: 417 2071 1084 2399 77 2106
Fragments arranged by size:
2399 2106 2071 1084 417 77
HaeII r_GCGC'y
Cuts at: 0 452 1499 1529 2618 5915 6757 8154
Size: 452 1047 30 1089 3297 842 1397
Fragments arranged by size:
3297 1397 1089 1047 842 452 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2227 2259 2658 2690 3297 3329 4810 4842
Size: 2227 32 399 32 607 32 1481 32
Cuts at: 4842 4971 5003 5500 5532 6801 6833 7779 7811
Size: 129 32 497 32 1269 32 946 32
Cuts at: 7811 8028 8060 8154
Size: 217 32 94
Fragments arranged by size:
2227 1481 1269 946 607 497 399 217
129 94 32 32 32 32 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 2150 3326 5197 5740 8154
Size: 2150 1176 1871 543 2414
Fragments arranged by size:
2414 2150 1871 1176 543
HindIII A'AGCT_T
Cuts at: 0 1593 2248 2267 8154
Size: 1593 655 19 5887
Fragments arranged by size:
5887 1593 655 19
KasI G'GCGC_C
Cuts at: 0 1495 1525 8154
Size: 1495 30 6629
Fragments arranged by size:
6629 1495 30
KpnI G_GTAC'C
Cuts at: 0 7222 8154
Size: 7222 932
MfeI C'AATT_G
Cuts at: 0 4681 8154
Size: 4681 3473
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 905 1826 4749 5417 7539 8154
Size: 905 921 2923 668 2122 615
Fragments arranged by size:
2923 2122 921 905 668 615
MscI TGG'CCA
Cuts at: 0 2218 8154
Size: 2218 5936
MslI CAynn'nnrTG
Cuts at: 0 857 2800 5081 5269 6332 6766 7246 7339
Size: 857 1943 2281 188 1063 434 480 93
Cuts at: 7339 8154
Size: 815
Fragments arranged by size:
2281 1943 1063 857 815 480 434 188
93
MspA1I CmG'CkG
Cuts at: 0 2307 4045 5506 5790 7012 7036 7821 8082
Size: 2307 1738 1461 284 1222 24 785 261
Cuts at: 8082 8154
Size: 72
Fragments arranged by size:
2307 1738 1461 1222 785 284 261 72
24
NarI GG'CG_CC
Cuts at: 0 1496 1526 8154
Size: 1496 30 6628
Fragments arranged by size:
6628 1496 30
NcoI C'CATG_G
Cuts at: 0 1529 1739 3308 5270 8154
Size: 1529 210 1569 1962 2884
Fragments arranged by size:
2884 1962 1569 1529 210
NdeI CA'TA_TG
Cuts at: 0 794 8154
Size: 794 7360
NheI G'CTAG_C
Cuts at: 0 6631 8154
Size: 6631 1523
NsiI A_TGCA'T
Cuts at: 0 538 8154
Size: 538 7616
NspI r_CATG'y
Cuts at: 0 1244 1824 3655 4255 6306 7736 8154
Size: 1244 580 1831 600 2051 1430 418
Fragments arranged by size:
2051 1831 1430 1244 600 580 418
PasI CC'CwG_GG
Cuts at: 0 1827 6749 8154
Size: 1827 4922 1405
Fragments arranged by size:
4922 1827 1405
PciI A'CATG_T
Cuts at: 0 1820 3651 8154
Size: 1820 1831 4503
Fragments arranged by size:
4503 1831 1820
PflMI CCAn_nnn'nTGG
Cuts at: 0 1053 1408 5790 8154
Size: 1053 355 4382 2364
Fragments arranged by size:
4382 2364 1053 355
PfoI T'CCnGG_A
Cuts at: 0 3485 3907 4068 8154
Size: 3485 422 161 4086
Fragments arranged by size:
4086 3485 422 161
PmlI CAC'GTG
Cuts at: 0 7929 8154
Size: 7929 225
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2394 2426 8154
Size: 2394 32 5728
Fragments arranged by size:
5728 2394 32
PpuMI rG'GwC_Cy
Cuts at: 0 1782 2644 3050 6669 6887 8154
Size: 1782 862 406 3619 218 1267
Fragments arranged by size:
3619 1782 1267 862 406 218
PsiI TTA'TAA
Cuts at: 0 977 7187 8154
Size: 977 6210 967
Fragments arranged by size:
6210 977 967
PspOMI G'GGCC_C
Cuts at: 0 3294 8154
Size: 3294 4860
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 7299 7331 8154
Size: 7299 32 823
Fragments arranged by size:
7299 823 32
PstI C_TGCA'G
Cuts at: 0 413 1541 1656 2133 3020 3380 4475 4922
Size: 413 1128 115 477 887 360 1095 447
Cuts at: 4922 7011 7568 7863 8154
Size: 2089 557 295 291
Fragments arranged by size:
2089 1128 1095 887 557 477 447 413
360 295 291 115
PvuII CAG'CTG
Cuts at: 0 4045 5506 5790 7012 8154
Size: 4045 1461 284 1222 1142
Fragments arranged by size:
4045 1461 1222 1142 284
SacI G_AGCT'C
Cuts at: 0 4859 7704 8154
Size: 4859 2845 450
Fragments arranged by size:
4859 2845 450
SacII CC_GC'GG
Cuts at: 0 2308 8083 8154
Size: 2308 5775 71
Fragments arranged by size:
5775 2308 71
SalI G'TCGA_C
Cuts at: 0 3324 5195 8154
Size: 3324 1871 2959
Fragments arranged by size:
3324 2959 1871
SapI GCTCTTCn'nnn_
Cuts at: 0 4370 4725 8154
Size: 4370 355 3429
Fragments arranged by size:
4370 3429 355
SbfI CC_TGCA'GG
Cuts at: 0 7863 8154
Size: 7863 291
ScaI AGT'ACT
Cuts at: 0 6681 8154
Size: 6681 1473
SexAI A'CCwGG_T
Cuts at: 0 2193 7775 8154
Size: 2193 5582 379
Fragments arranged by size:
5582 2193 379
SfcI C'TryA_G
Cuts at: 0 409 998 1537 1639 1652 2129 3016 3036
Size: 409 589 539 102 13 477 887 20
Cuts at: 3036 3376 4471 4918 5411 6872 7007 7099 7564
Size: 340 1095 447 493 1461 135 92 465
Cuts at: 7564 7760 7811 7859 8154
Size: 196 51 48 295
Fragments arranged by size:
1461 1095 887 589 539 493 477 465
447 409 340 295 196 135 102 92
51 48 20 13
SfoI GGC'GCC
Cuts at: 0 1497 1527 8154
Size: 1497 30 6627
Fragments arranged by size:
6627 1497 30
SmlI C'TyrA_G
Cuts at: 0 492 1716 2230 3669 4232 5841 6066 7073
Size: 492 1224 514 1439 563 1609 225 1007
Cuts at: 7073 8154
Size: 1081
Fragments arranged by size:
1609 1439 1224 1081 1007 563 514 492
225
SpeI A'CTAG_T
Cuts at: 0 4408 4974 8154
Size: 4408 566 3180
Fragments arranged by size:
4408 3180 566
SphI G_CATG'C
Cuts at: 0 7736 8154
Size: 7736 418
SspI AAT'ATT
Cuts at: 0 6197 7491 8154
Size: 6197 1294 663
Fragments arranged by size:
6197 1294 663
StuI AGG'CCT
Cuts at: 0 1004 3170 7427 7499 8154
Size: 1004 2166 4257 72 655
Fragments arranged by size:
4257 2166 1004 655 72
StyI C'CwwG_G
Cuts at: 0 1529 1739 2769 3308 4866 5270 6410 8154
Size: 1529 210 1030 539 1558 404 1140 1744
Fragments arranged by size:
1744 1558 1529 1140 1030 539 404 210
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 3849 4355 5797 7804 8154
Size: 3849 506 1442 2007 350
Fragments arranged by size:
3849 2007 1442 506 350
TatI w'GTAC_w
Cuts at: 0 4335 4411 5407 5662 6679 8154
Size: 4335 76 996 255 1017 1475
Fragments arranged by size:
4335 1475 1017 996 255 76
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1345 1377 3403 3435 4862 4894 5945 5977
Size: 1345 32 2026 32 1427 32 1051 32
Cuts at: 5977 6391 6423 8154
Size: 414 32 1731
Fragments arranged by size:
2026 1731 1427 1345 1051 414 32 32
32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 7791 8154
Size: 7791 363
XbaI T'CTAG_A
Cuts at: 0 6652 8154
Size: 6652 1502
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 7512 8154
Size: 7512 642
XhoI C'TCGA_G
Cuts at: 0 4232 8154
Size: 4232 3922
XmnI GAAnn'nnTTC
Cuts at: 0 2409 2517 3125 3170 6932 8154
Size: 2409 108 608 45 3762 1222
Fragments arranged by size:
3762 2409 1222 608 108 45
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
BsmBI Bsp1286I BspMI BsrBI BsrDI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI HaeII Hin4I
HincII HindIII KasI KpnI MfeI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NsiI NspI PasI
PciI PflMI PfoI PmlI PpiI PpuMI PsiI PspOMI
PsrI PstI PvuII SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfoI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmnI
Enzymes that do not cut:
AatII AfeI AgeI AhdI AscI AsiSI AvrII BsaWI
BsiEI BsiWI BspEI BspHI BsrFI BssHII BstZ17I EagI
EcoNI FseI FspI FspAI HpaI MluI NaeI NgoMIV
NotI NruI PacI PmeI PshAI PspXI PvuI RsrII
SanDI SfiI SgfI SgrAI SmaI SnaBI SrfI SwaI
XmaI ZraI