(Circular) (Six-base) MAPSORT of: pFN21AE0100.seq Check: 3316 from: 1 to: 7201 pFN21AE0100 7201 bp With 182 enzymes: SgfI * September 28, 2009 16:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2718 4207 2718 Size: 1489 5712 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5011 278 Size: 53 83 186 936 3475 2468 Fragments arranged by size: 3475 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2896 3380 4544 1937 Size: 959 484 1164 4594 Fragments arranged by size: 4594 1164 959 484 Acc65I G'GTAC_C Cuts at: 4522 4522 Size: 7201 AclI AA'CG_TT Cuts at: 1563 3564 5328 5701 1563 Size: 2001 1764 373 3063 Fragments arranged by size: 3063 2001 1764 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4450 5264 6648 6916 1632 Size: 2818 814 1384 268 1917 Fragments arranged by size: 2818 1917 1384 814 268 AfeI AGC'GCT Cuts at: 4237 4237 Size: 7201 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7004 AflIII A'CryG_T Cuts at: 1203 4350 6100 7077 1203 Size: 3147 1750 977 1327 Fragments arranged by size: 3147 1750 1327 977 AgeI A'CCGG_T Cuts at: 6032 6032 Size: 7201 AhdI GACnn_n'nnGTC Cuts at: 5930 5930 Size: 7201 AleI CACnn'nnGTG Cuts at: 1166 2918 1166 Size: 1752 5449 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7201 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3554 3586 4258 4290 3554 Size: 32 672 32 6465 Fragments arranged by size: 6465 672 32 32 AlwNI CAG_nnn'CTG Cuts at: 2180 4944 6516 2180 Size: 2764 1572 2865 Fragments arranged by size: 2865 2764 1572 ApaI G_GGCC'C Cuts at: 2070 2070 Size: 7201 ApaLI G'TGCA_C Cuts at: 5258 6414 5258 Size: 1156 6045 ApoI r'AATT_y Cuts at: 1493 2699 3047 3488 3977 4044 4457 4510 4736 Size: 1206 348 441 489 67 413 53 226 Cuts at: 4736 4888 6997 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1206 489 441 413 348 226 152 67 53 AseI AT'TA_AT Cuts at: 160 1032 3362 4490 5755 160 Size: 872 2330 1128 1265 1606 Fragments arranged by size: 2330 1606 1265 1128 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7201 AvaI C'yCGr_G Cuts at: 1870 1943 2069 2149 3333 4526 1870 Size: 73 126 80 1184 1193 4545 Fragments arranged by size: 4545 1193 1184 126 80 73 BamHI G'GATC_C Cuts at: 3963 4531 4898 3963 Size: 568 367 6266 Fragments arranged by size: 6266 568 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2153 2788 4522 5977 618 Size: 325 217 627 366 635 1734 1455 1842 Fragments arranged by size: 1842 1734 1455 635 627 366 325 217 BanII G_rGCy'C Cuts at: 1391 2070 2123 2150 4170 4520 1391 Size: 679 53 27 2020 350 4072 Fragments arranged by size: 4072 2020 679 350 53 27 BbeI G_GCGC'C Cuts at: 2157 2157 Size: 7201 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2809 2941 3712 928 Size: 971 910 132 771 4417 Fragments arranged by size: 4417 971 910 771 132 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5392 5426 1903 Size: 34 3455 34 3678 Fragments arranged by size: 3678 3455 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2828 3842 5093 6303 2828 Size: 1014 1251 1210 3726 Fragments arranged by size: 3726 1251 1210 1014 BclI T'GATC_A Cuts at: 1548 1548 Size: 7201 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2306 3141 5812 Size: 107 122 71 631 605 634 835 2671 Cuts at: 5812 136 Size: 1525 Fragments arranged by size: 2671 1525 835 634 631 605 122 107 71 BglII A'GATC_T Cuts at: 7196 7196 Size: 7201 BlpI GC'TnA_GC Cuts at: 4618 4618 Size: 7201 Bme1580I G_kGCm'C Cuts at: 1790 2070 2192 5262 6418 1790 Size: 280 122 3070 1156 2573 Fragments arranged by size: 3070 2573 1156 280 122 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7201 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4932 5890 442 Size: 416 53 83 399 24 3515 958 1753 Fragments arranged by size: 3515 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7201 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2297 2474 2804 3335 4196 4260 4472 5861 Size: 1058 177 330 531 861 64 212 1389 Cuts at: 5861 1239 Size: 2579 Fragments arranged by size: 2579 1389 1058 861 531 330 212 177 64 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2605 3233 3287 3684 4184 4319 5326 6191 6489 Size: 628 54 397 500 135 1007 865 298 Cuts at: 6489 2605 Size: 3317 Fragments arranged by size: 3317 1007 865 628 500 397 298 135 54 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3410 4167 5864 882 Size: 617 1911 757 1697 2219 Fragments arranged by size: 2219 1911 1697 757 617 BsaAI yAC'GTr Cuts at: 493 1204 3496 4351 4992 493 Size: 711 2292 855 641 2702 Fragments arranged by size: 2702 2292 855 711 641 BsaBI GATnn'nnATC Cuts at: 7195 7195 Size: 7201 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2154 5008 5390 6025 Size: 53 83 186 936 621 2854 382 635 Cuts at: 6025 275 Size: 1451 Fragments arranged by size: 2854 1451 936 635 621 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5634 6032 6306 6453 6864 5634 Size: 398 274 147 411 5971 Fragments arranged by size: 5971 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2447 2477 3223 3253 3554 Size: 30 165 30 1044 30 746 30 301 Cuts at: 3554 3584 4258 4288 1178 Size: 30 674 30 4091 Fragments arranged by size: 4091 1044 746 674 301 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2327 3332 1186 Size: 1141 1005 5055 Fragments arranged by size: 5055 1141 1005 BseYI C'CCAG_C Cuts at: 3176 6404 6900 3176 Size: 3228 496 3477 Fragments arranged by size: 3477 3228 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2711 1163 Size: 573 975 5653 Fragments arranged by size: 5653 975 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2196 4651 5412 6440 1540 Size: 275 186 195 2455 761 1028 2301 Fragments arranged by size: 2455 2301 1028 761 275 195 186 BsiHKAI G_wGCw'C Cuts at: 5262 5347 6418 5262 Size: 85 1071 6045 Fragments arranged by size: 6045 1071 85 BsmI GAATG_Cn' Cuts at: 3594 4110 4712 4805 3594 Size: 516 602 93 5990 Fragments arranged by size: 5990 602 516 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2070 2123 2150 2192 4170 4520 5262 Size: 399 280 53 27 42 1978 350 742 Cuts at: 5262 5347 6418 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1978 1071 742 399 350 280 85 53 42 27 BspEI T'CCGG_A Cuts at: 6864 6864 Size: 7201 BspHI T'CATG_A Cuts at: 5090 6820 5090 Size: 1730 5471 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2385 2718 4207 4556 844 Size: 1541 333 1489 349 3489 Fragments arranged by size: 3489 1541 1489 349 333 BsrBI CCG'CTC Cuts at: 1394 1823 2258 5088 1394 Size: 429 435 2830 3507 Fragments arranged by size: 3507 2830 435 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2933 3408 5696 5870 65 Size: 1183 1685 475 2288 174 1396 Fragments arranged by size: 2288 1685 1396 1183 475 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5845 6032 1700 Size: 110 4035 187 2869 Fragments arranged by size: 4035 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7201 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7201 BssSI C'ACGA_G Cuts at: 5261 6273 5261 Size: 1012 6189 BstAPI GCAn_nnn'nTGC Cuts at: 794 2180 794 Size: 1386 5815 BstBI TT'CG_AA Cuts at: 4048 6005 4048 Size: 1957 5244 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7201 BstXI CCAn_nnnn'nTGG Cuts at: 2275 3940 4450 2275 Size: 1665 510 5026 Fragments arranged by size: 5026 1665 510 BstYI r'GATC_y Cuts at: 1972 3534 3963 4309 4531 4898 5283 5300 6752 Size: 1562 429 346 222 367 385 17 1452 Cuts at: 6752 6838 6850 7196 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1562 1452 429 385 367 346 346 222 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2261 3959 4305 513 Size: 552 1196 1698 346 3409 Fragments arranged by size: 3409 1698 1196 552 346 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6462 Fragments arranged by size: 6462 683 56 BtsI GCAGTG_nn' Cuts at: 2181 4717 5511 5531 2181 Size: 2536 794 20 3851 Fragments arranged by size: 3851 2536 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7201 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7166 DraI TTT'AAA Cuts at: 4078 4505 4078 Size: 427 6774 DraIII CAC_nnn'GTG Cuts at: 2856 2856 Size: 7201 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6208 809 Size: 507 4892 1802 Fragments arranged by size: 4892 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2193 3441 3658 Size: 54 1425 50 275 18 363 1248 217 Cuts at: 3658 4648 5537 7166 8 Size: 990 889 1629 43 Fragments arranged by size: 1629 1425 1248 990 889 363 275 217 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2193 4648 1537 Size: 275 381 2455 4090 Fragments arranged by size: 4090 2455 381 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3127 3262 3462 4217 4256 5131 1353 Size: 1774 135 200 755 39 875 3423 Fragments arranged by size: 3423 1774 875 755 200 135 39 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2091 2287 5788 6162 6308 387 Size: 1064 138 502 196 3501 374 146 1280 Fragments arranged by size: 3501 1280 1064 502 374 196 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2297 2474 2804 3335 4196 4260 4450 Size: 393 665 177 330 531 861 64 190 Cuts at: 4450 4472 5264 5861 6648 6916 1239 Size: 22 792 597 787 268 1524 Fragments arranged by size: 1524 861 792 787 665 597 531 393 330 268 190 177 64 22 EcoNI CCTnn'n_nnAGG Cuts at: 4214 4214 Size: 7201 EcoRI G'AATT_C Cuts at: 4510 4888 4510 Size: 378 6823 FspI TGC'GCA Cuts at: 5707 5707 Size: 7201 HaeII r_GCGC'y Cuts at: 1678 2078 2099 2157 4239 6348 1678 Size: 400 21 58 2082 2109 2531 Fragments arranged by size: 2531 2109 2082 400 58 21 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2447 2479 2777 2809 3098 Size: 32 135 32 875 32 298 32 289 Cuts at: 3098 3130 3223 3255 3289 3321 3362 3394 5842 Size: 32 93 32 34 32 41 32 2448 Cuts at: 5842 5874 5916 5948 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2448 875 298 289 135 93 42 41 34 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4545 4791 669 Size: 988 281 2607 246 3079 Fragments arranged by size: 3079 2607 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7201 HpaI GTT'AAC Cuts at: 4791 4791 Size: 7201 KasI G'GCGC_C Cuts at: 2153 2153 Size: 7201 KpnI G_GTAC'C Cuts at: 4526 4526 Size: 7201 MfeI C'AATT_G Cuts at: 3843 4396 4800 3843 Size: 553 404 6244 Fragments arranged by size: 6244 553 404 MluI A'CGCG_T Cuts at: 7077 7077 Size: 7201 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2393 3444 6315 6499 1305 Size: 1088 1051 2871 184 2007 Fragments arranged by size: 2871 2007 1088 1051 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5379 Fragments arranged by size: 5379 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2918 2957 3938 5159 5518 5677 518 Size: 648 1752 39 981 1221 359 159 2042 Fragments arranged by size: 2042 1752 1221 981 648 359 159 39 MspA1I CmG'CkG Cuts at: 1606 2135 2186 2263 3580 4617 5294 6442 6687 Size: 529 51 77 1317 1037 677 1148 245 Cuts at: 6687 1606 Size: 2120 Fragments arranged by size: 2120 1317 1148 1037 677 529 245 77 51 NarI GG'CG_CC Cuts at: 2154 2154 Size: 7201 NcoI C'CATG_G Cuts at: 513 1065 4305 513 Size: 552 3240 3409 Fragments arranged by size: 3409 3240 552 NdeI CA'TA_TG Cuts at: 387 3826 387 Size: 3439 3762 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7201 NotI GC'GGCC_GC Cuts at: 4648 4648 Size: 7201 NspI r_CATG'y Cuts at: 1132 1229 4559 6104 1132 Size: 97 3330 1545 2229 Fragments arranged by size: 3330 2229 1545 97 PacI TTA_AT'TAA Cuts at: 3651 3651 Size: 7201 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7201 PciI A'CATG_T Cuts at: 6100 6100 Size: 7201 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7201 PfoI T'CCnGG_A Cuts at: 2973 4114 4276 4449 2973 Size: 1141 162 173 5725 Fragments arranged by size: 5725 1141 173 162 PmeI GTTT'AAAC Cuts at: 4505 4505 Size: 7201 PmlI CAC'GTG Cuts at: 3496 3496 Size: 7201 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3554 3586 4258 4290 5270 5302 6783 6815 3554 Size: 32 672 32 980 32 1481 32 3940 Fragments arranged by size: 3940 1481 980 672 32 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7201 PsiI TTA'TAA Cuts at: 4295 4771 4295 Size: 476 6725 PspOMI G'GGCC_C Cuts at: 2066 2066 Size: 7201 PstI C_TGCA'G Cuts at: 830 2185 2382 3400 4553 830 Size: 1355 197 1018 1153 3478 Fragments arranged by size: 3478 1355 1153 1018 197 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7201 PvuII CAG'CTG Cuts at: 3580 3580 Size: 7201 RsrII CG'GwC_CG Cuts at: 2162 2162 Size: 7201 SacII CC_GC'GG Cuts at: 2264 2264 Size: 7201 SalI G'TCGA_C Cuts at: 1936 4543 1936 Size: 2607 4594 SapI GCTCTTCn'nnn_ Cuts at: 3462 4217 3462 Size: 755 6446 SbfI CC_TGCA'GG Cuts at: 4553 4553 Size: 7201 ScaI AGT'ACT Cuts at: 5449 7161 5449 Size: 1712 5489 SfcI C'TryA_G Cuts at: 826 1045 2181 2378 3396 3557 4101 4549 5684 Size: 219 1136 197 1018 161 544 448 1135 Cuts at: 5684 6365 6556 826 Size: 681 191 1471 Fragments arranged by size: 1471 1136 1135 1018 681 544 448 219 197 191 161 SfoI GGC'GCC Cuts at: 2155 2155 Size: 7201 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7201 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7201 SmaI CCC'GGG Cuts at: 1872 2071 4528 1872 Size: 199 2457 4545 Fragments arranged by size: 4545 2457 199 SmlI C'TyrA_G Cuts at: 820 1017 1943 2584 3212 3266 3699 4199 4334 Size: 197 926 641 628 54 433 500 135 Cuts at: 4334 5305 6206 6468 820 Size: 971 901 262 1553 Fragments arranged by size: 1553 971 926 901 641 628 500 433 262 197 135 54 SnaBI TAC'GTA Cuts at: 493 4992 493 Size: 4499 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7201 SphI G_CATG'C Cuts at: 1132 4559 1132 Size: 3427 3774 SspI AAT'ATT Cuts at: 5 52 5125 5 Size: 47 5073 2081 Fragments arranged by size: 5073 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7201 StyI C'CwwG_G Cuts at: 513 1065 4305 4640 513 Size: 552 3240 335 3074 Fragments arranged by size: 3240 3074 552 335 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2371 2935 5228 5245 5398 5583 6028 Size: 1255 791 564 2293 17 153 185 445 Cuts at: 6028 325 Size: 1498 Fragments arranged by size: 2293 1498 1255 791 564 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2292 2694 4407 Size: 274 80 33 51 1443 315 402 1713 Cuts at: 4407 5447 7159 96 Size: 1040 1712 138 Fragments arranged by size: 1713 1712 1443 1040 402 315 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2601 2633 4129 4161 7161 7193 2601 Size: 32 1496 32 3000 32 2609 Fragments arranged by size: 3000 2609 1496 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7201 XbaI T'CTAG_A Cuts at: 4537 4537 Size: 7201 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7201 XmaI C'CCGG_G Cuts at: 1870 2069 4526 1870 Size: 199 2457 4545 Fragments arranged by size: 4545 2457 199 XmnI GAAnn'nnTTC Cuts at: 3566 4110 4364 4460 5330 3566 Size: 544 254 96 870 5437 Fragments arranged by size: 5437 870 544 254 96 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5009 276 Size: 53 83 186 936 3475 2468 Fragments arranged by size: 3475 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PpuMI PspXI PsrI SacI SanDI SexAI SfiI SrfI SwaI XcmI