(Linear) (Six-base) MAPSORT of: KIEE4286.seq Check: 8458 from: 1 to: 4893 KIEE4286.orf With 182 enzymes: SgfI * July 3, 2012 18:56 .. AccI GT'mk_AC Cuts at: 0 416 740 4893 Size: 416 324 4153 Fragments arranged by size: 4153 416 324 Acc65I G'GTAC_C Cuts at: 0 275 998 1691 4893 Size: 275 723 693 3202 Fragments arranged by size: 3202 723 693 275 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 163 549 947 2644 3472 4659 4893 Size: 163 386 398 1697 828 1187 234 Fragments arranged by size: 1697 1187 828 398 386 234 163 AfeI AGC'GCT Cuts at: 0 124 4893 Size: 124 4769 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 101 2264 4893 Size: 101 2163 2629 Fragments arranged by size: 2629 2163 101 AlwNI CAG_nnn'CTG Cuts at: 0 99 1044 2670 3223 4069 4479 4626 4893 Size: 99 945 1626 553 846 410 147 267 Fragments arranged by size: 1626 945 846 553 410 267 147 99 ApaI G_GGCC'C Cuts at: 0 22 1359 2403 4893 Size: 22 1337 1044 2490 Fragments arranged by size: 2490 1337 1044 22 ApoI r'AATT_y Cuts at: 0 496 3416 3472 4893 Size: 496 2920 56 1421 Fragments arranged by size: 2920 1421 496 56 AvaI C'yCGr_G Cuts at: 0 407 1367 2117 3325 4893 Size: 407 960 750 1208 1568 Fragments arranged by size: 1568 1208 960 750 407 BamHI G'GATC_C Cuts at: 0 1743 4893 Size: 1743 3150 BanI G'GyrC_C Cuts at: 0 275 998 1691 3765 4893 Size: 275 723 693 2074 1128 Fragments arranged by size: 2074 1128 723 693 275 BanII G_rGCy'C Cuts at: 0 22 599 888 925 1310 1359 1959 2403 Size: 22 577 289 37 385 49 600 444 Cuts at: 2403 3600 3932 4893 Size: 1197 332 961 Fragments arranged by size: 1197 961 600 577 444 385 332 289 49 37 22 BbsI GAAGACnn'nnnn_ Cuts at: 0 1630 3200 4893 Size: 1630 1570 1693 Fragments arranged by size: 1693 1630 1570 BbvCI CC'TCA_GC Cuts at: 0 53 506 1421 2609 4893 Size: 53 453 915 1188 2284 Fragments arranged by size: 2284 1188 915 453 53 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 3348 3382 4893 Size: 3348 34 1511 Fragments arranged by size: 3348 1511 34 BclI T'GATC_A Cuts at: 0 1113 3906 4893 Size: 1113 2793 987 Fragments arranged by size: 2793 1113 987 BglI GCCn_nnn'nGGC Cuts at: 0 883 1363 2225 3628 4893 Size: 883 480 862 1403 1265 Fragments arranged by size: 1403 1265 883 862 480 BglII A'GATC_T Cuts at: 0 251 4656 4893 Size: 251 4405 237 Fragments arranged by size: 4405 251 237 BlpI GC'TnA_GC Cuts at: 0 2252 2833 3205 3638 4619 4893 Size: 2252 581 372 433 981 274 Fragments arranged by size: 2252 981 581 433 372 274 Bme1580I G_kGCm'C Cuts at: 0 22 1207 1359 2403 3153 4001 4893 Size: 22 1185 152 1044 750 848 892 Fragments arranged by size: 1185 1044 892 848 750 152 22 BmgBI CAC'GTC Cuts at: 0 3081 4893 Size: 3081 1812 BmrI ACTGGGnnnn_n' Cuts at: 0 692 4893 Size: 692 4201 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1347 1844 4893 Size: 1347 497 3049 Fragments arranged by size: 3049 1347 497 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 130 193 535 730 1024 1208 1258 1279 Size: 130 63 342 195 294 184 50 21 Cuts at: 1279 1381 1407 1769 2194 2362 2614 2692 2872 Size: 102 26 362 425 168 252 78 180 Cuts at: 2872 2976 3004 3025 3736 3901 3955 4780 4893 Size: 104 28 21 711 165 54 825 113 Fragments arranged by size: 825 711 425 362 342 294 252 195 184 180 168 165 130 113 104 102 78 63 54 50 28 26 21 21 Bpu10I CC'TnA_GC Cuts at: 0 53 506 1421 1504 2609 4009 4480 4893 Size: 53 453 915 83 1105 1400 471 413 Fragments arranged by size: 1400 1105 915 471 453 413 83 53 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 922 1786 3336 3715 4138 4893 Size: 922 864 1550 379 423 755 Fragments arranged by size: 1550 922 864 755 423 379 BsaI GGTCTCn'nnnn_ Cuts at: 0 367 2751 4893 Size: 367 2384 2142 Fragments arranged by size: 2384 2142 367 BsaHI Gr'CG_yC Cuts at: 0 526 2436 3482 3563 4221 4893 Size: 526 1910 1046 81 658 672 Fragments arranged by size: 1910 1046 672 658 526 81 BsaWI w'CCGG_w Cuts at: 0 1163 2723 4245 4893 Size: 1163 1560 1522 648 Fragments arranged by size: 1560 1522 1163 648 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1186 1216 2116 2146 2974 3004 3347 3377 Size: 1186 30 900 30 828 30 343 30 Cuts at: 3377 4150 4180 4893 Size: 773 30 713 Fragments arranged by size: 1186 900 828 773 713 343 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 21 70 127 163 190 993 1318 1690 Size: 21 49 57 36 27 803 325 372 Cuts at: 1690 1783 2137 2537 3355 3793 4519 4858 4893 Size: 93 354 400 818 438 726 339 35 Fragments arranged by size: 818 803 726 438 400 372 354 339 325 93 57 49 36 35 27 21 BseYI C'CCAG_C Cuts at: 0 26 210 453 1044 1214 1442 2283 3543 Size: 26 184 243 591 170 228 841 1260 Cuts at: 3543 3611 4893 Size: 68 1282 Fragments arranged by size: 1282 1260 841 591 243 228 184 170 68 26 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1162 2425 4893 Size: 1162 1263 2468 Fragments arranged by size: 2468 1263 1162 BsiHKAI G_wGCw'C Cuts at: 0 599 705 1274 1310 3206 3887 3932 4105 Size: 599 106 569 36 1896 681 45 173 Cuts at: 4105 4486 4893 Size: 381 407 Fragments arranged by size: 1896 681 599 569 407 381 173 106 45 36 BsmI GAATG_Cn' Cuts at: 0 2256 4893 Size: 2256 2637 BsmBI CGTCTCn'nnnn_ Cuts at: 0 3330 4893 Size: 3330 1563 Bsp1286I G_dGCh'C Cuts at: 0 22 599 705 888 925 1207 1274 1310 Size: 22 577 106 183 37 282 67 36 Cuts at: 1310 1359 1959 2403 3153 3206 3600 3887 3932 Size: 49 600 444 750 53 394 287 45 Cuts at: 3932 4001 4105 4486 4893 Size: 69 104 381 407 Fragments arranged by size: 750 600 577 444 407 394 381 287 282 183 106 104 69 67 53 49 45 37 36 22 BspEI T'CCGG_A Cuts at: 0 1163 4893 Size: 1163 3730 BspHI T'CATG_A Cuts at: 0 4817 4893 Size: 4817 76 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1526 2891 4337 4893 Size: 1526 1365 1446 556 Fragments arranged by size: 1526 1446 1365 556 BsrBI CCG'CTC Cuts at: 0 1887 4268 4893 Size: 1887 2381 625 Fragments arranged by size: 2381 1887 625 BsrDI GCAATG_nn' Cuts at: 0 3896 4893 Size: 3896 997 BsrFI r'CCGG_y Cuts at: 0 104 4893 Size: 104 4789 BsrGI T'GTAC_A Cuts at: 0 2978 3836 4231 4893 Size: 2978 858 395 662 Fragments arranged by size: 2978 858 662 395 BstAPI GCAn_nnn'nTGC Cuts at: 0 622 784 4893 Size: 622 162 4109 Fragments arranged by size: 4109 622 162 BstXI CCAn_nnnn'nTGG Cuts at: 0 286 1416 2907 2955 3831 4743 4893 Size: 286 1130 1491 48 876 912 150 Fragments arranged by size: 1491 1130 912 876 286 150 48 BstYI r'GATC_y Cuts at: 0 251 1743 1772 2069 2126 2297 2387 2477 Size: 251 1492 29 297 57 171 90 90 Cuts at: 2477 2651 3939 4160 4656 4782 4893 Size: 174 1288 221 496 126 111 Fragments arranged by size: 1492 1288 496 297 251 221 174 171 126 111 90 90 57 29 Bsu36I CC'TnA_GG Cuts at: 0 1208 2431 4893 Size: 1208 1223 2462 Fragments arranged by size: 2462 1223 1208 BtgI C'CryG_G Cuts at: 0 233 377 879 1970 2223 3581 3830 3845 Size: 233 144 502 1091 253 1358 249 15 Cuts at: 3845 4893 Size: 1048 Fragments arranged by size: 1358 1091 1048 502 253 249 233 144 15 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 3333 3969 4893 Size: 3333 636 924 Fragments arranged by size: 3333 924 636 BtsI GCAGTG_nn' Cuts at: 0 656 1274 1913 2033 2150 2751 2969 3797 Size: 656 618 639 120 117 601 218 828 Cuts at: 3797 4252 4610 4893 Size: 455 358 283 Fragments arranged by size: 828 656 639 618 601 455 358 283 218 120 117 DraI TTT'AAA Cuts at: 0 2361 2825 4893 Size: 2361 464 2068 Fragments arranged by size: 2361 2068 464 DraIII CAC_nnn'GTG Cuts at: 0 1140 4893 Size: 1140 3753 DrdI GACnn_nn'nnGTC Cuts at: 0 3496 4893 Size: 3496 1397 EaeI y'GGCC_r Cuts at: 0 203 536 587 1296 2909 3440 3578 3821 Size: 203 333 51 709 1613 531 138 243 Cuts at: 3821 4118 4397 4893 Size: 297 279 496 Fragments arranged by size: 1613 709 531 496 333 297 279 243 203 138 51 EarI CTCTTCn'nnn_ Cuts at: 0 445 451 710 1315 1553 2630 2881 4607 Size: 445 6 259 605 238 1077 251 1726 Cuts at: 4607 4831 4893 Size: 224 62 Fragments arranged by size: 1726 1077 605 445 259 251 238 224 62 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1765 4452 4893 Size: 1765 2687 441 Fragments arranged by size: 2687 1765 441 EcoICRI GAG'CTC Cuts at: 0 597 1308 3930 4893 Size: 597 711 2622 963 Fragments arranged by size: 2622 963 711 597 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 130 163 193 535 549 730 947 1024 Size: 130 33 30 342 14 181 217 77 Cuts at: 1024 1208 1258 1279 1381 1407 1769 2194 2362 Size: 184 50 21 102 26 362 425 168 Cuts at: 2362 2614 2644 2692 2872 2976 3004 3025 3472 Size: 252 30 48 180 104 28 21 447 Cuts at: 3472 3736 3901 3955 4659 4780 4893 Size: 264 165 54 704 121 113 Fragments arranged by size: 704 447 425 362 342 264 252 217 184 181 180 168 165 130 121 113 104 102 77 54 50 48 33 30 30 28 26 21 21 14 EcoNI CCTnn'n_nnAGG Cuts at: 0 145 838 1409 1420 4630 4893 Size: 145 693 571 11 3210 263 Fragments arranged by size: 3210 693 571 263 145 11 EcoO109I rG'GnC_Cy Cuts at: 0 942 1356 1572 2273 2679 3699 4476 4893 Size: 942 414 216 701 406 1020 777 417 Fragments arranged by size: 1020 942 777 701 417 414 406 216 EcoRV GAT'ATC Cuts at: 0 4125 4893 Size: 4125 768 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 2937 4512 4656 4893 Size: 2937 1575 144 237 Fragments arranged by size: 2937 1575 237 144 HaeII r_GCGC'y Cuts at: 0 126 3618 4364 4893 Size: 126 3492 746 529 Fragments arranged by size: 3492 746 529 126 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 462 494 1728 1760 2116 2148 2289 2321 Size: 462 32 1234 32 356 32 141 32 Cuts at: 2321 3332 3364 3787 3819 4150 4182 4893 Size: 1011 32 423 32 331 32 711 Fragments arranged by size: 1234 1011 711 462 423 356 331 141 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 873 2463 3861 4851 4893 Size: 873 1590 1398 990 42 Fragments arranged by size: 1590 1398 990 873 42 HpaI GTT'AAC Cuts at: 0 2463 4893 Size: 2463 2430 KpnI G_GTAC'C Cuts at: 0 279 1002 1695 4893 Size: 279 723 693 3198 Fragments arranged by size: 3198 723 693 279 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 4103 4893 Size: 4103 790 MscI TGG'CCA Cuts at: 0 205 538 589 1298 2911 3442 3580 3823 Size: 205 333 51 709 1613 531 138 243 Cuts at: 3823 4399 4893 Size: 576 494 Fragments arranged by size: 1613 709 576 531 494 333 243 205 138 51 MslI CAynn'nnrTG Cuts at: 0 3844 4893 Size: 3844 1049 MspA1I CmG'CkG Cuts at: 0 16 52 270 505 555 628 1011 1146 Size: 16 36 218 235 50 73 383 135 Cuts at: 1146 1218 1257 1336 1654 2137 2328 2670 2787 Size: 72 39 79 318 483 191 342 117 Cuts at: 2787 3024 3547 3589 4113 4209 4311 4893 Size: 237 523 42 524 96 102 582 Fragments arranged by size: 582 524 523 483 383 342 318 237 235 218 191 135 117 102 96 79 73 72 50 42 39 36 16 NaeI GCC'GGC Cuts at: 0 106 4893 Size: 106 4787 NcoI C'CATG_G Cuts at: 0 879 1970 3581 3830 4893 Size: 879 1091 1611 249 1063 Fragments arranged by size: 1611 1091 1063 879 249 NdeI CA'TA_TG Cuts at: 0 3291 4893 Size: 3291 1602 NgoMIV G'CCGG_C Cuts at: 0 104 4893 Size: 104 4789 NspI r_CATG'y Cuts at: 0 405 2536 4893 Size: 405 2131 2357 Fragments arranged by size: 2357 2131 405 PasI CC'CwG_GG Cuts at: 0 3624 4347 4893 Size: 3624 723 546 Fragments arranged by size: 3624 723 546 PflMI CCAn_nnn'nTGG Cuts at: 0 125 1785 3081 3624 4893 Size: 125 1660 1296 543 1269 Fragments arranged by size: 1660 1296 1269 543 125 PfoI T'CCnGG_A Cuts at: 0 1746 2630 3041 3179 3932 4625 4893 Size: 1746 884 411 138 753 693 268 Fragments arranged by size: 1746 884 753 693 411 268 138 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1910 1942 4893 Size: 1910 32 2951 Fragments arranged by size: 2951 1910 32 PpuMI rG'GwC_Cy Cuts at: 0 1572 2273 2679 3699 4893 Size: 1572 701 406 1020 1194 Fragments arranged by size: 1572 1194 1020 701 406 PspOMI G'GGCC_C Cuts at: 0 18 1355 2399 4893 Size: 18 1337 1044 2494 Fragments arranged by size: 2494 1337 1044 18 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 3508 3540 4893 Size: 3508 32 1353 Fragments arranged by size: 3508 1353 32 PstI C_TGCA'G Cuts at: 0 618 1098 1523 1653 2057 2738 3158 3993 Size: 618 480 425 130 404 681 420 835 Cuts at: 3993 4463 4793 4893 Size: 470 330 100 Fragments arranged by size: 835 681 618 480 470 425 420 404 330 130 100 PvuII CAG'CTG Cuts at: 0 52 270 505 555 628 1011 1218 1257 Size: 52 218 235 50 73 383 207 39 Cuts at: 1257 1336 1654 2137 2670 2787 3024 3547 3589 Size: 79 318 483 533 117 237 523 42 Cuts at: 3589 4113 4209 4893 Size: 524 96 684 Fragments arranged by size: 684 533 524 523 483 383 318 237 235 218 207 117 96 79 73 52 50 42 39 SacI G_AGCT'C Cuts at: 0 599 1310 3932 4893 Size: 599 711 2622 961 Fragments arranged by size: 2622 961 711 599 SapI GCTCTTCn'nnn_ Cuts at: 0 710 1315 4893 Size: 710 605 3578 Fragments arranged by size: 3578 710 605 SbfI CC_TGCA'GG Cuts at: 0 1523 3158 4893 Size: 1523 1635 1735 Fragments arranged by size: 1735 1635 1523 ScaI AGT'ACT Cuts at: 0 3802 4893 Size: 3802 1091 SfcI C'TryA_G Cuts at: 0 614 1094 1519 1649 2053 2517 2734 3154 Size: 614 480 425 130 404 464 217 420 Cuts at: 3154 3646 3989 4459 4521 4789 4893 Size: 492 343 470 62 268 104 Fragments arranged by size: 614 492 480 470 464 425 420 404 343 268 217 130 104 62 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1363 4893 Size: 1363 3530 SmaI CCC'GGG Cuts at: 0 409 1369 2119 4893 Size: 409 960 750 2774 Fragments arranged by size: 2774 960 750 409 SmlI C'TyrA_G Cuts at: 0 937 1765 3315 3730 4153 4893 Size: 937 828 1550 415 423 740 Fragments arranged by size: 1550 937 828 740 423 415 SphI G_CATG'C Cuts at: 0 2536 4893 Size: 2536 2357 StuI AGG'CCT Cuts at: 0 136 1415 2395 3007 3313 3670 4228 4893 Size: 136 1279 980 612 306 357 558 665 Fragments arranged by size: 1279 980 665 612 558 357 306 136 StyI C'CwwG_G Cuts at: 0 879 1970 2144 3581 3830 4400 4893 Size: 879 1091 174 1437 249 570 493 Fragments arranged by size: 1437 1091 879 570 493 249 174 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 337 1480 4735 4893 Size: 337 1143 3255 158 Fragments arranged by size: 3255 1143 337 158 TatI w'GTAC_w Cuts at: 0 2978 3524 3800 3836 4231 4529 4893 Size: 2978 546 276 36 395 298 364 Fragments arranged by size: 2978 546 395 364 298 276 36 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1186 1218 4893 Size: 1186 32 3675 Fragments arranged by size: 3675 1186 32 Tth111I GACn'n_nGTC Cuts at: 0 1570 1629 3223 4893 Size: 1570 59 1594 1670 Fragments arranged by size: 1670 1594 1570 59 XbaI T'CTAG_A Cuts at: 0 888 4893 Size: 888 4005 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 126 705 2667 2952 3421 4113 4893 Size: 126 579 1962 285 469 692 780 Fragments arranged by size: 1962 780 692 579 469 285 126 XmaI C'CCGG_G Cuts at: 0 407 1367 2117 4893 Size: 407 960 750 2776 Fragments arranged by size: 2776 960 750 407 XmnI GAAnn'nnTTC Cuts at: 0 4658 4825 4893 Size: 4658 167 68 Fragments arranged by size: 4658 167 68 Enzymes that do cut: AccI Acc65I AcuI AfeI AlfI AlwNI ApaI ApoI AvaI BamHI BanI BanII BbsI BbvCI BcgI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII Hin4I HincII HpaI KpnI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NspI PasI PflMI PfoI PpiI PpuMI PspOMI PsrI PstI PvuII SacI SapI SbfI ScaI SfcI SfiI SmaI SmlI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AclI AflII AflIII AgeI AhdI AleI AloI ApaLI AscI AseI AsiSI AvrII BaeI BbeI BciVI BfrBI BmtI BsaAI BsaBI BsiEI BsiWI BssHII BssSI BstBI BstEII BstZ17I ClaI CspCI EagI EcoRI FseI FspI FspAI HindIII KasI MfeI MluI NarI NheI NotI NruI NsiI PacI PciI PmeI PmlI PshAI PsiI PspXI PvuI RsrII SacII SalI SanDI SexAI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI XhoI ZraI