(Circular) (Six-base) MAPSORT of: pFN21AA1526.seq Check: 96 from: 1 to: 7426 pFN21AA1526 7426 bp With 182 enzymes: SgfI * October 20, 2009 10:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3309 3309 Size: 7426 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3081 4209 5236 278 Size: 53 83 186 936 1545 1128 1027 2468 Fragments arranged by size: 2468 1545 1128 1027 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4769 1937 Size: 2832 4594 Acc65I G'GTAC_C Cuts at: 4747 4747 Size: 7426 AclI AA'CG_TT Cuts at: 1563 5553 5926 1563 Size: 3990 373 3063 Fragments arranged by size: 3990 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2449 2648 2964 5489 6873 7141 1632 Size: 817 199 316 2525 1384 268 1917 Fragments arranged by size: 2525 1917 1384 817 316 268 199 AfeI AGC'GCT Cuts at: 2139 2139 Size: 7426 AflII C'TTAA_G Cuts at: 820 1017 3041 820 Size: 197 2024 5205 Fragments arranged by size: 5205 2024 197 AflIII A'CryG_T Cuts at: 1203 2505 6325 7302 1203 Size: 1302 3820 977 1327 Fragments arranged by size: 3820 1327 1302 977 AgeI A'CCGG_T Cuts at: 6257 6257 Size: 7426 AhdI GACnn_n'nnGTC Cuts at: 2026 2552 2866 6155 2026 Size: 526 314 3289 3297 Fragments arranged by size: 3297 3289 526 314 AleI CACnn'nnGTG Cuts at: 1166 2711 2769 2927 3107 4566 1166 Size: 1545 58 158 180 1459 4026 Fragments arranged by size: 4026 1545 1459 180 158 58 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4397 1184 Size: 3213 4213 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3118 3150 3118 Size: 32 7394 AlwNI CAG_nnn'CTG Cuts at: 2569 2942 3730 4100 4522 5169 6741 2569 Size: 373 788 370 422 647 1572 3254 Fragments arranged by size: 3254 1572 788 647 422 373 370 ApaI G_GGCC'C Cuts at: 3227 3227 Size: 7426 ApaLI G'TGCA_C Cuts at: 5483 6639 5483 Size: 1156 6270 ApoI r'AATT_y Cuts at: 1493 4735 4961 5113 7222 1493 Size: 3242 226 152 2109 1697 Fragments arranged by size: 3242 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5980 160 Size: 872 4948 1606 Fragments arranged by size: 4948 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7426 AvaI C'yCGr_G Cuts at: 1870 1943 2595 2746 3536 3853 4751 1870 Size: 73 652 151 790 317 898 4545 Fragments arranged by size: 4545 898 790 652 317 151 73 AvrII C'CTAG_G Cuts at: 4526 4526 Size: 7426 BamHI G'GATC_C Cuts at: 4756 5123 4756 Size: 367 7059 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2268 2771 3054 3297 3446 Size: 325 217 627 481 503 283 243 149 Cuts at: 3446 3602 3899 4502 4747 6202 618 Size: 156 297 603 245 1455 1842 Fragments arranged by size: 1842 1455 627 603 503 481 325 297 283 245 243 217 156 149 BanII G_rGCy'C Cuts at: 1391 2039 2063 2483 2636 2964 3227 4440 4745 Size: 648 24 420 153 328 263 1213 305 Cuts at: 4745 1391 Size: 4072 Fragments arranged by size: 4072 1213 648 420 328 305 263 153 24 BbeI G_GCGC'C Cuts at: 2272 4506 2272 Size: 2234 5192 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2209 3856 928 Size: 971 310 1647 4498 Fragments arranged by size: 4498 1647 971 310 BbvCI CC'TCA_GC Cuts at: 3491 3491 Size: 7426 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2200 2234 4474 4508 5617 5651 1903 Size: 34 263 34 2240 34 1109 34 3678 Fragments arranged by size: 3678 2240 1109 263 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5318 6528 5318 Size: 1210 6216 BclI T'GATC_A Cuts at: 1548 1548 Size: 7426 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2621 3231 3329 Size: 107 122 71 631 605 949 610 98 Cuts at: 3329 4010 4097 6037 136 Size: 681 87 1940 1525 Fragments arranged by size: 1940 1525 949 681 631 610 605 122 107 98 87 71 BglII A'GATC_T Cuts at: 7421 7421 Size: 7426 BlpI GC'TnA_GC Cuts at: 2153 4555 4843 2153 Size: 2402 288 4736 Fragments arranged by size: 4736 2402 288 Bme1580I G_kGCm'C Cuts at: 1790 2776 3227 3860 3902 5487 6643 1790 Size: 986 451 633 42 1585 1156 2573 Fragments arranged by size: 2573 1585 1156 986 633 451 42 BmgBI CAC'GTC Cuts at: 1318 4443 1318 Size: 3125 4301 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5157 6115 442 Size: 416 53 83 399 24 3740 958 1753 Fragments arranged by size: 3740 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7426 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3839 3839 Size: 7426 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3302 3665 3809 4454 6086 1239 Size: 2063 363 144 645 1632 2579 Fragments arranged by size: 2579 2063 1632 645 363 144 Bpu10I CC'TnA_GC Cuts at: 3248 3491 3248 Size: 243 7183 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2948 4562 5551 6416 6714 2948 Size: 1614 989 865 298 3660 Fragments arranged by size: 3660 1614 989 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3110 3143 3568 6089 882 Size: 617 1611 33 425 2521 2219 Fragments arranged by size: 2521 2219 1611 617 425 33 BsaAI yAC'GTr Cuts at: 493 1204 2506 4591 5217 493 Size: 711 1302 2085 626 2702 Fragments arranged by size: 2702 2085 1302 711 626 BsaBI GATnn'nnATC Cuts at: 7420 7420 Size: 7426 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2269 3078 4002 4206 Size: 53 83 186 936 736 809 924 204 Cuts at: 4206 4503 5233 5615 6250 275 Size: 297 730 382 635 1451 Fragments arranged by size: 1451 936 924 809 736 730 635 382 297 204 186 83 53 BsaWI w'CCGG_w Cuts at: 2257 2796 5859 6257 6531 6678 7089 2257 Size: 539 3063 398 274 147 411 2594 Fragments arranged by size: 3063 2594 539 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3539 3569 3590 3620 3968 Size: 30 165 30 2136 30 21 30 348 Cuts at: 3968 3998 4427 4457 1178 Size: 30 429 30 4147 Fragments arranged by size: 4147 2136 429 348 165 30 30 30 30 30 30 21 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2815 3299 3419 3913 1186 Size: 1629 484 120 494 4699 Fragments arranged by size: 4699 1629 494 484 120 BseYI C'CCAG_C Cuts at: 2056 2757 2854 3226 3542 3808 3980 4100 4257 Size: 701 97 372 316 266 172 120 157 Cuts at: 4257 6629 7125 2056 Size: 2372 496 2357 Fragments arranged by size: 2372 2357 701 496 372 316 266 172 157 120 97 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2114 2795 3912 1163 Size: 573 378 681 1117 4677 Fragments arranged by size: 4677 1117 681 573 378 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2070 2337 2866 4876 5637 6665 Size: 275 186 69 267 529 2010 761 1028 Cuts at: 6665 1540 Size: 2301 Fragments arranged by size: 2301 2010 1028 761 529 275 267 186 69 BsiHKAI G_wGCw'C Cuts at: 2039 2490 2807 4635 5487 5572 6643 2039 Size: 451 317 1828 852 85 1071 2822 Fragments arranged by size: 2822 1828 1071 852 451 317 85 BsiWI C'GTAC_G Cuts at: 2119 2119 Size: 7426 BsmI GAATG_Cn' Cuts at: 4937 5030 4937 Size: 93 7333 BsmBI CGTCTCn'nnnn_ Cuts at: 3370 3970 3370 Size: 600 6826 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2039 2063 2483 2490 2636 2776 2807 Size: 399 249 24 420 7 146 140 31 Cuts at: 2807 2964 3227 3860 3902 4440 4635 4745 5487 Size: 157 263 633 42 538 195 110 742 Cuts at: 5487 5572 6643 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 633 538 420 399 263 249 195 157 146 140 110 85 42 31 24 7 BspEI T'CCGG_A Cuts at: 7089 7089 Size: 7426 BspHI T'CATG_A Cuts at: 5315 7045 5315 Size: 1730 5696 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2358 3309 4781 844 Size: 1514 951 1472 3489 Fragments arranged by size: 3489 1514 1472 951 BsrBI CCG'CTC Cuts at: 1394 1823 2167 5313 1394 Size: 429 344 3146 3507 Fragments arranged by size: 3507 3146 429 344 BsrDI GCAATG_nn' Cuts at: 65 1248 5921 6095 65 Size: 1183 4673 174 1396 Fragments arranged by size: 4673 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2048 2865 3230 6070 6257 1700 Size: 110 238 817 365 2840 187 2869 Fragments arranged by size: 2869 2840 817 365 238 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7426 BssHII G'CGCG_C Cuts at: 1925 2204 1925 Size: 279 7147 BssSI C'ACGA_G Cuts at: 2450 3270 5486 6498 2450 Size: 820 2216 1012 3378 Fragments arranged by size: 3378 2216 1012 820 BstAPI GCAn_nnn'nTGC Cuts at: 794 2818 794 Size: 2024 5402 BstBI TT'CG_AA Cuts at: 6230 6230 Size: 7426 BstEII G'GTnAC_C Cuts at: 1184 2599 2792 3241 1184 Size: 1415 193 449 5369 Fragments arranged by size: 5369 1415 449 193 BstXI CCAn_nnnn'nTGG Cuts at: 2644 2644 Size: 7426 BstYI r'GATC_y Cuts at: 1972 3713 4756 5123 5508 5525 6977 7063 7075 Size: 1741 1043 367 385 17 1452 86 12 Cuts at: 7075 7421 1972 Size: 346 1977 Fragments arranged by size: 1977 1741 1452 1043 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2777 2777 Size: 7426 BtgI C'CryG_G Cuts at: 513 1065 2473 2764 2887 3153 3327 3352 3558 Size: 552 1408 291 123 266 174 25 206 Cuts at: 3558 3598 3699 4046 513 Size: 40 101 347 3893 Fragments arranged by size: 3893 1408 552 347 291 266 206 174 123 101 40 25 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4471 487 Size: 683 56 3245 3442 Fragments arranged by size: 3442 3245 683 56 BtsI GCAGTG_nn' Cuts at: 2182 3746 4942 5736 5756 2182 Size: 1564 1196 794 20 3852 Fragments arranged by size: 3852 1564 1196 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7426 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7391 DraI TTT'AAA Cuts at: 4730 4730 Size: 7426 DraIII CAC_nnn'GTG Cuts at: 2494 3487 2494 Size: 993 6433 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2258 3029 4700 6433 809 Size: 507 942 771 1671 1733 1802 Fragments arranged by size: 1802 1733 1671 942 771 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2067 2334 2863 Size: 54 1425 50 275 18 237 267 529 Cuts at: 2863 3624 4132 4473 4873 5762 7391 8 Size: 761 508 341 400 889 1629 43 Fragments arranged by size: 1629 1425 889 761 529 508 400 341 275 267 237 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2067 2334 2863 4873 1537 Size: 275 255 267 529 2010 4090 Fragments arranged by size: 4090 2010 529 275 267 255 EarI CTCTTCn'nnn_ Cuts at: 1353 2324 3949 5356 1353 Size: 971 1625 1407 3423 Fragments arranged by size: 3423 1625 1407 971 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2177 3013 6013 6387 6533 387 Size: 1064 138 588 836 3000 374 146 1280 Fragments arranged by size: 3000 1280 1064 836 588 374 146 138 EcoICRI GAG'CTC Cuts at: 2037 2037 Size: 7426 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2449 2648 2964 3302 3665 3809 4454 Size: 393 817 199 316 338 363 144 645 Cuts at: 4454 5489 6086 6873 7141 1239 Size: 1035 597 787 268 1524 Fragments arranged by size: 1524 1035 817 787 645 597 393 363 338 316 268 199 144 EcoNI CCTnn'n_nnAGG Cuts at: 3434 3434 Size: 7426 EcoO109I rG'GnC_Cy Cuts at: 2341 3223 3376 3533 3820 3918 2341 Size: 882 153 157 287 98 5849 Fragments arranged by size: 5849 882 287 157 153 98 EcoRI G'AATT_C Cuts at: 4735 5113 4735 Size: 378 7048 FspI TGC'GCA Cuts at: 2231 5932 2231 Size: 3701 3725 FspAI rTGC'GCAy Cuts at: 2231 2231 Size: 7426 HaeII r_GCGC'y Cuts at: 1678 2141 2272 4226 4329 4506 6573 1678 Size: 463 131 1954 103 177 2067 2531 Fragments arranged by size: 2531 2067 1954 463 177 131 103 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2012 2044 2538 2570 2852 Size: 32 135 32 440 32 494 32 282 Cuts at: 2852 2884 2924 2956 3491 3523 3539 3571 3698 Size: 32 40 32 535 32 16 32 127 Cuts at: 3698 3730 3926 3958 4427 4459 6067 6099 6141 Size: 32 196 32 469 32 1608 32 42 Cuts at: 6141 6173 1373 Size: 32 2626 Fragments arranged by size: 2626 1608 535 494 469 440 282 196 135 127 42 40 32 32 32 32 32 32 32 32 32 32 32 32 32 16 HincII GTy'rAC Cuts at: 669 1657 1938 2578 4021 4770 5016 669 Size: 988 281 640 1443 749 246 3079 Fragments arranged by size: 3079 1443 988 749 640 281 246 HindIII A'AGCT_T Cuts at: 748 3736 748 Size: 2988 4438 HpaI GTT'AAC Cuts at: 5016 5016 Size: 7426 KasI G'GCGC_C Cuts at: 2268 4502 2268 Size: 2234 5192 KpnI G_GTAC'C Cuts at: 4751 4751 Size: 7426 MfeI C'AATT_G Cuts at: 5025 5025 Size: 7426 MluI A'CGCG_T Cuts at: 7302 7302 Size: 7426 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2331 6540 6724 1305 Size: 1026 4209 184 2007 Fragments arranged by size: 4209 2007 1026 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4134 10 Size: 54 1425 343 2302 3302 Fragments arranged by size: 3302 2302 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2504 2711 2769 2927 3107 4566 5384 Size: 648 1338 207 58 158 180 1459 818 Cuts at: 5384 5743 5902 518 Size: 359 159 2042 Fragments arranged by size: 2042 1459 1338 818 648 359 207 180 159 158 58 MspA1I CmG'CkG Cuts at: 1606 2017 2128 2314 2958 3896 3997 4272 4842 Size: 411 111 186 644 938 101 275 570 Cuts at: 4842 5519 6667 6912 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 938 677 644 570 411 275 245 186 111 101 NaeI GCC'GGC Cuts at: 3232 3232 Size: 7426 NarI GG'CG_CC Cuts at: 2269 4503 2269 Size: 2234 5192 NcoI C'CATG_G Cuts at: 513 1065 3153 3327 3558 513 Size: 552 2088 174 231 4381 Fragments arranged by size: 4381 2088 552 231 174 NdeI CA'TA_TG Cuts at: 387 387 Size: 7426 NgoMIV G'CCGG_C Cuts at: 3230 3230 Size: 7426 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7426 NotI GC'GGCC_GC Cuts at: 4873 4873 Size: 7426 NspI r_CATG'y Cuts at: 1132 1229 4784 6329 1132 Size: 97 3555 1545 2229 Fragments arranged by size: 3555 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2678 2872 3724 3914 4480 1348 Size: 1330 194 852 190 566 4294 Fragments arranged by size: 4294 1330 852 566 194 190 PciI A'CATG_T Cuts at: 6325 6325 Size: 7426 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7426 PfoI T'CCnGG_A Cuts at: 3137 3137 Size: 7426 PmeI GTTT'AAAC Cuts at: 4730 4730 Size: 7426 PmlI CAC'GTG Cuts at: 4591 4591 Size: 7426 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5495 5527 7008 7040 5495 Size: 32 1481 32 5881 Fragments arranged by size: 5881 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3820 3918 3820 Size: 98 7328 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7426 PsiI TTA'TAA Cuts at: 4996 4996 Size: 7426 PspOMI G'GGCC_C Cuts at: 3223 3223 Size: 7426 PstI C_TGCA'G Cuts at: 830 4087 4778 830 Size: 3257 691 3478 Fragments arranged by size: 3478 3257 691 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7426 PvuII CAG'CTG Cuts at: 2128 2314 2128 Size: 186 7240 SacI G_AGCT'C Cuts at: 2039 2039 Size: 7426 SalI G'TCGA_C Cuts at: 1936 4768 1936 Size: 2832 4594 SapI GCTCTTCn'nnn_ Cuts at: 2324 2324 Size: 7426 SbfI CC_TGCA'GG Cuts at: 4778 4778 Size: 7426 ScaI AGT'ACT Cuts at: 5674 7386 5674 Size: 1712 5714 SexAI A'CCwGG_T Cuts at: 2223 2847 3483 2223 Size: 624 636 6166 Fragments arranged by size: 6166 636 624 SfcI C'TryA_G Cuts at: 826 1045 2368 3568 4083 4774 5909 6590 6781 Size: 219 1323 1200 515 691 1135 681 191 Cuts at: 6781 826 Size: 1471 Fragments arranged by size: 1471 1323 1200 1135 691 681 515 219 191 SfoI GGC'GCC Cuts at: 2270 4504 2270 Size: 2234 5192 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7426 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7426 SmaI CCC'GGG Cuts at: 1872 2597 3538 4753 1872 Size: 725 941 1215 4545 Fragments arranged by size: 4545 1215 941 725 SmlI C'TyrA_G Cuts at: 820 1017 1943 2963 3041 4577 5530 6431 6693 Size: 197 926 1020 78 1536 953 901 262 Cuts at: 6693 820 Size: 1553 Fragments arranged by size: 1553 1536 1020 953 926 901 262 197 78 SnaBI TAC'GTA Cuts at: 493 5217 493 Size: 4724 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7426 SphI G_CATG'C Cuts at: 1132 4784 1132 Size: 3652 3774 SspI AAT'ATT Cuts at: 5 52 5350 5 Size: 47 5298 2081 Fragments arranged by size: 5298 2081 47 StuI AGG'CCT Cuts at: 1517 4664 1517 Size: 3147 4279 StyI C'CwwG_G Cuts at: 513 1065 2442 2908 3153 3327 3558 3772 3800 Size: 552 1377 466 245 174 231 214 28 Cuts at: 3800 4526 4865 513 Size: 726 339 3074 Fragments arranged by size: 3074 1377 726 552 466 339 245 231 214 174 28 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2195 2701 3425 3922 4152 5453 5470 Size: 1255 615 506 724 497 230 1301 17 Cuts at: 5470 5623 5808 6253 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1301 1255 724 615 506 497 445 230 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2658 5672 7384 Size: 274 80 33 51 1443 681 3014 1712 Cuts at: 7384 96 Size: 138 Fragments arranged by size: 3014 1712 1443 681 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3590 3622 7386 7418 3590 Size: 32 3764 32 3598 Fragments arranged by size: 3764 3598 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7426 XbaI T'CTAG_A Cuts at: 2983 4762 2983 Size: 1779 5647 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2053 2287 2765 3634 3994 4447 2053 Size: 234 478 869 360 453 5032 Fragments arranged by size: 5032 869 478 453 360 234 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7426 XmaI C'CCGG_G Cuts at: 1870 2595 3536 4751 1870 Size: 725 941 1215 4545 Fragments arranged by size: 4545 1215 941 725 XmnI GAAnn'nnTTC Cuts at: 5555 5555 Size: 7426 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3079 4207 5234 276 Size: 53 83 186 936 1545 1128 1027 2468 Fragments arranged by size: 2468 1545 1128 1027 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI BaeI BfrBI BstZ17I EcoRV FalI FseI NruI NsiI PacI PspXI PsrI RsrII SacII SanDI SfiI SrfI SwaI