(Circular) (Six-base) MAPSORT of: pFN21AA0382.seq Check: 9795 from: 1 to: 9337 pFN21AA0382 9337 bp With 182 enzymes: SgfI * April 15, 2010 09:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2704 5733 2704 Size: 3029 6308 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7147 278 Size: 53 83 186 936 5611 2468 Fragments arranged by size: 5611 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2021 6680 1937 Size: 84 4659 4594 Fragments arranged by size: 4659 4594 84 Acc65I G'GTAC_C Cuts at: 6658 6658 Size: 9337 AclI AA'CG_TT Cuts at: 1563 7464 7837 1563 Size: 5901 373 3063 Fragments arranged by size: 5901 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2502 3999 4442 4802 5021 7400 8784 9052 Size: 870 1497 443 360 219 2379 1384 268 Cuts at: 9052 1632 Size: 1917 Fragments arranged by size: 2379 1917 1497 1384 870 443 360 268 219 AfeI AGC'GCT Cuts at: 6440 6440 Size: 9337 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9140 AflIII A'CryG_T Cuts at: 1203 3554 5121 5226 8236 9213 1203 Size: 2351 1567 105 3010 977 1327 Fragments arranged by size: 3010 2351 1567 1327 977 105 AgeI A'CCGG_T Cuts at: 8168 8168 Size: 9337 AhdI GACnn_n'nnGTC Cuts at: 8066 8066 Size: 9337 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 9337 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 9337 AlwNI CAG_nnn'CTG Cuts at: 2780 2858 3977 4091 5357 5519 5791 7080 8652 Size: 78 1119 114 1266 162 272 1289 1572 Cuts at: 8652 2780 Size: 3465 Fragments arranged by size: 3465 1572 1289 1266 1119 272 162 114 78 ApaLI G'TGCA_C Cuts at: 7394 8550 7394 Size: 1156 8181 ApoI r'AATT_y Cuts at: 1493 2625 2955 3694 4467 4494 5038 5767 6422 Size: 1132 330 739 773 27 544 729 655 Cuts at: 6422 6646 6872 7024 9133 1493 Size: 224 226 152 2109 1697 Fragments arranged by size: 2109 1697 1132 773 739 729 655 544 330 226 224 152 27 AscI GG'CGCG_CC Cuts at: 3875 3875 Size: 9337 AseI AT'TA_AT Cuts at: 160 1032 4380 7891 160 Size: 872 3348 3511 1606 Fragments arranged by size: 3511 3348 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9337 AvaI C'yCGr_G Cuts at: 1870 1943 6662 1870 Size: 73 4719 4545 Fragments arranged by size: 4719 4545 73 BamHI G'GATC_C Cuts at: 6667 7034 6667 Size: 367 8970 BanI G'GyrC_C Cuts at: 618 943 1160 1787 6034 6658 8113 618 Size: 325 217 627 4247 624 1455 1842 Fragments arranged by size: 4247 1842 1455 627 624 325 217 BanII G_rGCy'C Cuts at: 1391 3369 3629 4408 5034 6656 1391 Size: 1978 260 779 626 1622 4072 Fragments arranged by size: 4072 1978 1622 779 626 260 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2168 2272 3364 3996 4766 5142 5838 Size: 971 269 104 1092 632 770 376 696 Cuts at: 5838 928 Size: 4427 Fragments arranged by size: 4427 1092 971 770 696 632 376 269 104 BbvCI CC'TCA_GC Cuts at: 2745 2745 Size: 9337 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7528 7562 1903 Size: 34 5591 34 3678 Fragments arranged by size: 5591 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3974 4465 7229 8439 3974 Size: 491 2764 1210 4872 Fragments arranged by size: 4872 2764 1210 491 BclI T'GATC_A Cuts at: 1548 4432 4695 1548 Size: 2884 263 6190 Fragments arranged by size: 6190 2884 263 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2318 7948 136 Size: 107 122 71 631 605 646 5630 1525 Fragments arranged by size: 5630 1525 646 631 605 122 107 71 BglII A'GATC_T Cuts at: 3343 4911 9332 3343 Size: 1568 4421 3348 Fragments arranged by size: 4421 3348 1568 BlpI GC'TnA_GC Cuts at: 6754 6754 Size: 9337 Bme1580I G_kGCm'C Cuts at: 1790 7398 8554 1790 Size: 5608 1156 2573 Fragments arranged by size: 5608 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 9337 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2879 2904 4349 Size: 416 53 83 399 24 1462 25 1445 Cuts at: 4349 6266 7068 8026 442 Size: 1917 802 958 1753 Fragments arranged by size: 1917 1753 1462 1445 958 802 416 399 83 53 25 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9337 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2343 2408 2562 2794 2880 4113 4978 7997 Size: 1104 65 154 232 86 1233 865 3019 Cuts at: 7997 1239 Size: 2579 Fragments arranged by size: 3019 2579 1233 1104 865 232 154 86 65 Bpu10I CC'TnA_GC Cuts at: 2745 2784 3363 3603 3885 5033 2745 Size: 39 579 240 282 1148 7049 Fragments arranged by size: 7049 1148 579 282 240 39 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3293 7462 8327 8625 3293 Size: 4169 865 298 4005 Fragments arranged by size: 4169 4005 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3814 4568 8000 882 Size: 617 2315 754 3432 2219 Fragments arranged by size: 3432 2315 2219 754 617 BsaAI yAC'GTr Cuts at: 493 1204 5621 7128 493 Size: 711 4417 1507 2702 Fragments arranged by size: 4417 2702 1507 711 BsaBI GATnn'nnATC Cuts at: 3384 5554 6177 9331 3384 Size: 2170 623 3154 3390 Fragments arranged by size: 3390 3154 2170 623 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2443 7144 7526 8161 Size: 53 83 186 936 910 4701 382 635 Cuts at: 8161 275 Size: 1451 Fragments arranged by size: 4701 1451 936 910 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 7770 8168 8442 8589 9000 7770 Size: 398 274 147 411 8107 Fragments arranged by size: 8107 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2784 2814 3663 3693 1178 Size: 30 165 30 1381 30 849 30 6822 Fragments arranged by size: 6822 1381 849 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2143 2195 2520 4187 4649 5510 6311 6366 Size: 957 52 325 1667 462 861 801 55 Cuts at: 6366 6475 1186 Size: 109 4048 Fragments arranged by size: 4048 1667 957 861 801 462 325 109 55 52 BseYI C'CCAG_C Cuts at: 3797 6441 8540 9036 3797 Size: 2644 2099 496 4098 Fragments arranged by size: 4098 2644 2099 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3130 3579 4187 6296 6513 1163 Size: 573 1394 449 608 2109 217 3987 Fragments arranged by size: 3987 2109 1394 608 573 449 217 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6787 7548 8576 1540 Size: 275 186 4786 761 1028 2301 Fragments arranged by size: 4786 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2583 3369 3629 3705 4408 5034 6144 7398 7483 Size: 786 260 76 703 626 1110 1254 85 Cuts at: 7483 8554 2583 Size: 1071 3366 Fragments arranged by size: 3366 1254 1110 1071 786 703 626 260 85 76 BsmI GAATG_Cn' Cuts at: 6848 6941 6848 Size: 93 9244 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2583 3369 3629 3705 4408 5034 6144 Size: 399 793 786 260 76 703 626 1110 Cuts at: 6144 6656 7398 7483 8554 1391 Size: 512 742 85 1071 2174 Fragments arranged by size: 2174 1110 1071 793 786 742 703 626 512 399 260 85 76 BspEI T'CCGG_A Cuts at: 9000 9000 Size: 9337 BspHI T'CATG_A Cuts at: 2520 3832 5068 7226 8956 2520 Size: 1312 1236 2158 1730 2901 Fragments arranged by size: 2901 2158 1730 1312 1236 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2704 3993 5733 6692 844 Size: 1860 1289 1740 959 3489 Fragments arranged by size: 3489 1860 1740 1289 959 BsrBI CCG'CTC Cuts at: 1394 1823 3550 7224 1394 Size: 429 1727 3674 3507 Fragments arranged by size: 3674 3507 1727 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2118 5967 7832 8006 65 Size: 1183 870 3849 1865 174 1396 Fragments arranged by size: 3849 1865 1396 1183 870 174 BsrFI r'CCGG_y Cuts at: 1700 1810 7981 8168 1700 Size: 110 6171 187 2869 Fragments arranged by size: 6171 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 9337 BssHII G'CGCG_C Cuts at: 1925 3875 1925 Size: 1950 7387 BssSI C'ACGA_G Cuts at: 7397 8409 7397 Size: 1012 8325 BstAPI GCAn_nnn'nTGC Cuts at: 794 5756 794 Size: 4962 4375 BstBI TT'CG_AA Cuts at: 4225 4414 4561 8141 4225 Size: 189 147 3580 5421 Fragments arranged by size: 5421 3580 189 147 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 9337 BstXI CCAn_nnnn'nTGG Cuts at: 3805 4308 5085 3805 Size: 503 777 8057 Fragments arranged by size: 8057 777 503 BstYI r'GATC_y Cuts at: 1972 2189 2718 2854 3343 3379 4911 5476 6471 Size: 217 529 136 489 36 1532 565 995 Cuts at: 6471 6667 7034 7419 7436 8888 8974 8986 9332 Size: 196 367 385 17 1452 86 12 346 Cuts at: 9332 1972 Size: 1977 Fragments arranged by size: 1977 1532 1452 995 565 529 489 385 367 346 217 196 136 86 36 17 12 Bsu36I CC'TnA_GG Cuts at: 3375 3924 3375 Size: 549 8788 BtgI C'CryG_G Cuts at: 513 1065 3815 3858 5987 6014 6105 513 Size: 552 2750 43 2129 27 91 3745 Fragments arranged by size: 3745 2750 2129 552 91 43 27 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3371 487 Size: 683 56 2145 6453 Fragments arranged by size: 6453 2145 683 56 BtsI GCAGTG_nn' Cuts at: 2896 6853 7647 7667 2896 Size: 3957 794 20 4566 Fragments arranged by size: 4566 3957 794 20 ClaI AT'CG_AT Cuts at: 1554 3298 4587 1554 Size: 1744 1289 6304 Fragments arranged by size: 6304 1744 1289 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4815 4850 529 Size: 35 4251 35 5016 Fragments arranged by size: 5016 4251 35 35 DraI TTT'AAA Cuts at: 6641 6641 Size: 9337 DraIII CAC_nnn'GTG Cuts at: 6170 6170 Size: 9337 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2276 3828 8344 809 Size: 507 960 1552 4516 1802 Fragments arranged by size: 4516 1802 1552 960 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4862 6784 7673 Size: 54 1425 50 275 18 3032 1922 889 Cuts at: 7673 9302 8 Size: 1629 43 Fragments arranged by size: 3032 1922 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6784 1537 Size: 275 4972 4090 Fragments arranged by size: 4972 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3781 5022 5813 6207 7267 1353 Size: 2428 1241 791 394 1060 3423 Fragments arranged by size: 3423 2428 1241 1060 791 394 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7924 8298 8444 387 Size: 1064 138 6335 374 146 1280 Fragments arranged by size: 6335 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3367 3627 4406 5032 3367 Size: 260 779 626 7672 Fragments arranged by size: 7672 779 626 260 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2343 2408 2502 2562 2794 2880 3999 Size: 393 711 65 94 60 232 86 1119 Cuts at: 3999 4113 4442 4802 4978 5021 7400 7997 8784 Size: 114 329 360 176 43 2379 597 787 Cuts at: 8784 9052 1239 Size: 268 1524 Fragments arranged by size: 2379 1524 1119 787 711 597 393 360 329 268 232 176 114 94 86 65 60 43 EcoNI CCTnn'n_nnAGG Cuts at: 5454 5454 Size: 9337 EcoO109I rG'GnC_Cy Cuts at: 3809 4020 5377 3809 Size: 211 1357 7769 Fragments arranged by size: 7769 1357 211 EcoRI G'AATT_C Cuts at: 4467 6646 7024 4467 Size: 2179 378 6780 Fragments arranged by size: 6780 2179 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4535 5159 6140 4535 Size: 624 981 7732 Fragments arranged by size: 7732 981 624 FspI TGC'GCA Cuts at: 7843 7843 Size: 9337 HaeII r_GCGC'y Cuts at: 1678 4963 5325 6442 8484 1678 Size: 3285 362 1117 2042 2531 Fragments arranged by size: 3285 2531 2042 1117 362 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2138 2170 2174 2188 2206 Size: 32 135 32 566 32 4 14 18 Cuts at: 2206 2220 2323 2355 2515 2547 2747 2779 2944 Size: 14 103 32 160 32 200 32 165 Cuts at: 2944 2976 2996 3028 3320 3351 3352 3383 3586 Size: 32 20 32 292 31 1 31 203 Cuts at: 3586 3611 3618 3643 4046 4078 4687 4719 6167 Size: 25 7 25 403 32 609 32 1448 Cuts at: 6167 6199 6463 6495 6594 6608 6626 6640 7978 Size: 32 264 32 99 14 18 14 1338 Cuts at: 7978 8010 8052 8084 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1448 1338 609 566 403 292 264 203 200 165 160 135 103 99 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 31 31 25 25 20 18 18 14 14 14 14 7 4 1 HincII GTy'rAC Cuts at: 669 1657 1938 5445 6205 6681 6927 669 Size: 988 281 3507 760 476 246 3079 Fragments arranged by size: 3507 3079 988 760 476 281 246 HindIII A'AGCT_T Cuts at: 748 5289 748 Size: 4541 4796 HpaI GTT'AAC Cuts at: 6205 6927 6205 Size: 722 8615 KpnI G_GTAC'C Cuts at: 6662 6662 Size: 9337 MfeI C'AATT_G Cuts at: 3846 6936 3846 Size: 3090 6247 MluI A'CGCG_T Cuts at: 9213 9213 Size: 9337 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3001 3695 4054 4815 5369 5668 8451 8635 Size: 1696 694 359 761 554 299 2783 184 Cuts at: 8635 1305 Size: 2007 Fragments arranged by size: 2783 2007 1696 761 694 554 359 299 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4864 10 Size: 54 1425 343 3032 4483 Fragments arranged by size: 4483 3032 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2351 3656 4802 5306 5667 5928 6083 Size: 648 1185 1305 1146 504 361 261 155 Cuts at: 6083 6171 6393 7295 7654 7813 518 Size: 88 222 902 359 159 2042 Fragments arranged by size: 2042 1305 1185 1146 902 648 504 361 359 261 222 159 155 88 MspA1I CmG'CkG Cuts at: 1606 2749 3114 4623 5213 6753 7430 8578 8823 Size: 1143 365 1509 590 1540 677 1148 245 Cuts at: 8823 1606 Size: 2120 Fragments arranged by size: 2120 1540 1509 1148 1143 677 590 365 245 NcoI C'CATG_G Cuts at: 513 1065 3858 6105 513 Size: 552 2793 2247 3745 Fragments arranged by size: 3745 2793 2247 552 NdeI CA'TA_TG Cuts at: 387 2508 5940 387 Size: 2121 3432 3784 Fragments arranged by size: 3784 3432 2121 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9337 NotI GC'GGCC_GC Cuts at: 6784 6784 Size: 9337 NspI r_CATG'y Cuts at: 1132 1229 3558 4652 6695 8240 1132 Size: 97 2329 1094 2043 1545 2229 Fragments arranged by size: 2329 2229 2043 1545 1094 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 9337 PciI A'CATG_T Cuts at: 3554 8236 3554 Size: 4682 4655 PflMI CCAn_nnn'nTGG Cuts at: 1108 6514 6595 1108 Size: 5406 81 3850 Fragments arranged by size: 5406 3850 81 PfoI T'CCnGG_A Cuts at: 2857 2857 Size: 9337 PmeI GTTT'AAAC Cuts at: 6641 6641 Size: 9337 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4395 4427 5851 5883 7406 7438 8919 8951 4395 Size: 32 1424 32 1523 32 1481 32 4781 Fragments arranged by size: 4781 1523 1481 1424 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3809 5377 3809 Size: 1568 7769 PshAI GACnn'nnGTC Cuts at: 1613 2509 1613 Size: 896 8441 PsiI TTA'TAA Cuts at: 6907 6907 Size: 9337 PstI C_TGCA'G Cuts at: 830 3343 4897 6324 6689 830 Size: 2513 1554 1427 365 3478 Fragments arranged by size: 3478 2513 1554 1427 365 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9337 PvuII CAG'CTG Cuts at: 2749 3114 5213 2749 Size: 365 2099 6873 Fragments arranged by size: 6873 2099 365 SacI G_AGCT'C Cuts at: 3369 3629 4408 5034 3369 Size: 260 779 626 7672 Fragments arranged by size: 7672 779 626 260 SalI G'TCGA_C Cuts at: 1936 6679 1936 Size: 4743 4594 SanDI GG'GwC_CC Cuts at: 3809 3809 Size: 9337 SapI GCTCTTCn'nnn_ Cuts at: 5022 5813 5022 Size: 791 8546 SbfI CC_TGCA'GG Cuts at: 6689 6689 Size: 9337 ScaI AGT'ACT Cuts at: 7585 9297 7585 Size: 1712 7625 SfcI C'TryA_G Cuts at: 826 1045 2663 2796 2884 3339 3609 3866 4008 Size: 219 1618 133 88 455 270 257 142 Cuts at: 4008 4893 5208 6320 6685 7820 8501 8692 826 Size: 885 315 1112 365 1135 681 191 1471 Fragments arranged by size: 1618 1471 1135 1112 885 681 455 365 315 270 257 219 191 142 133 88 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9337 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9337 SmaI CCC'GGG Cuts at: 1872 6664 1872 Size: 4792 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3272 7441 8342 8604 820 Size: 197 926 1329 4169 901 262 1553 Fragments arranged by size: 4169 1553 1329 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 7128 493 Size: 6635 2702 SpeI A'CTAG_T Cuts at: 152 2570 152 Size: 2418 6919 SphI G_CATG'C Cuts at: 1132 6695 1132 Size: 5563 3774 SspI AAT'ATT Cuts at: 5 52 4843 5911 7261 5 Size: 47 4791 1068 1350 2081 Fragments arranged by size: 4791 2081 1350 1068 47 StuI AGG'CCT Cuts at: 1517 5918 1517 Size: 4401 4936 StyI C'CwwG_G Cuts at: 513 1065 3480 3858 6105 6776 513 Size: 552 2415 378 2247 671 3074 Fragments arranged by size: 3074 2415 2247 671 552 378 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3543 4318 6371 7364 7381 7534 7719 Size: 1255 1963 775 2053 993 17 153 185 Cuts at: 7719 8164 325 Size: 445 1498 Fragments arranged by size: 2053 1963 1498 1255 993 775 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2326 2866 5252 Size: 274 80 33 51 1443 349 540 2386 Cuts at: 5252 6519 7583 9295 96 Size: 1267 1064 1712 138 Fragments arranged by size: 2386 1712 1443 1267 1064 540 349 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2352 2384 3663 3695 9297 9329 2352 Size: 32 1279 32 5602 32 2360 Fragments arranged by size: 5602 2360 1279 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2167 5888 1316 Size: 851 3721 4765 Fragments arranged by size: 4765 3721 851 XbaI T'CTAG_A Cuts at: 2962 3292 3346 6673 2962 Size: 330 54 3327 5626 Fragments arranged by size: 5626 3327 330 54 XcmI CCAnnnn_n'nnnnTGG Cuts at: 6596 6596 Size: 9337 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9337 XmaI C'CCGG_G Cuts at: 1870 6662 1870 Size: 4792 4545 XmnI GAAnn'nnTTC Cuts at: 2991 3450 4467 4515 5139 7466 2991 Size: 459 1017 48 624 2327 4862 Fragments arranged by size: 4862 2327 1017 624 459 48 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7145 276 Size: 53 83 186 936 5611 2468 Fragments arranged by size: 5611 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI