Restriction map for
pFN21AB5204
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# NO CUT ENZYMES ( 156 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AfaI AflII AflIII AgeI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspHI
AspI AviII AvrII BalI BamHI
BbeI BbrPI BbvCI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrGI BssHII
Bst1107I BstBI BstF5I BstNI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII DrdI EagI EclXI
Eco47III Eco52I Eco81I EcoNI EcoRII
EcoRV EcoT22I FbaI FokI FseI
FspI HgaI Hin1I HindIII KasI
KpnI KspI MaeI MaeII MamI
MfeI MflI MluI MluNI MroI
MscI MunI MvaI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tth111I Van91I
XbaI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AhdI 346 GACCCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 609 GATCC GGATC(4/5)
ApaI 475 GGGCCC GGGCC^C
AspEI 346 GACCCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 416 GGCACC G^G[CT][GA]CC
BanII 475 GGGCCC G[GA]GC[CT]^C
BbsI 189 GAAGAC GAAGAC(2/6)
BpuAI 189 GAAGAC GAAGAC(2/6)
BsrFI 419 ACCGGC [GA]^CCGG[CT]
BstAPI 75 GCAGCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 726 GGTCACC G^GT[ACGT]ACC
BstPI 726 GGTCACC G^GT[ACGT]ACC
BstXI 174 CCACATCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 419 ACCGGC [GA]^CCGG[CT]
DdeI 796 CTCAG C^T[ACGT]AG
EaeI 712 CGGCCA [CT]^GGCC[GA]
Eam1105I 346 GACCCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 630 CTCTTC CTCTTC(1/4)
EcoO65I 726 GGTCACC G^GT[ACGT]ACC
EcoRI 124 GAATTC G^AATTC
HaeII 109 GGCGCT [GA]GCGC^[CT]
HincII 615 GTTAAC GT[CT]^[GA]AC
HindII 615 GTTAAC GT[CT]^[GA]AC
HpaI 615 GTTAAC GTT^AAC
Ksp632I 630 CTCTTC CTCTTC(1/4)
MseI 616 TTAA T^TAA
NcoI 515 CCATGG C^CATGG
NspI 158 GCATGT [GA]CATG^[CT]
PpuMI 705 GGGACCC [GA]G^G[AT]CC[CT]
PstI 411 CTGCAG CTGCA^G
PvuII 64 CAGCTG CAG^CTG
SfcI 411 CTGCAG C^T[GA][CT]AG
TfiI 461 GATTC G^A[AT]TC
Tru9I 616 TTAA T^TAA
Tsp45I 727 GTCAC ^GT[GC]AC
XcmI 776 CCAAAGGGCACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccII 51 CGCG CG^CG
693 CGCG
AcsI 124 GAATTC [GA]^AATT[CT]
487 AAATTC
AluI 65 AGCT AG^CT
138 AGCT
AlwNI 61 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
76 CAGCCGCTG
ApoI 124 GAATTC [GA]^AATT[CT]
487 AAATTC
AvaI 280 CCCGAG C^[CT]CG[GA]G
349 CCCGAG
BmyI 475 GGGCCC G[AGT]GC[ACT]^C
781 GGGCAC
BpmI 560 CTCCAG CTGGAG(16/14)
718 CTGGAG
BsgI 337 CTGCAC GTGCAG(16/14)
312 GTGCAG
BsoBI 280 CCCGAG C^[CT]CG[GA]G
349 CCCGAG
Bsp1286I 475 GGGCCC G[AGT]GC[ACT]^C
781 GGGCAC
BspMI 657 GCAGGT ACCTGC(4/8)
723 GCAGGT
BssSI 454 CTCGTG CACGAG(-5/-1)
480 CTCGTG
BstUI 51 CGCG CG^CG
693 CGCG
DsaI 384 CCGTGG C^C[GA][CT]GG
515 CCATGG
EcoT14I 373 CCTTGG C^C[AT][AT]GG
515 CCATGG
FauI 654 CCCGC CCCGC(4/6)
694 GCGGG
HphI 184 GGTGA GGTGA(8/7)
728 TCACC
MboII 189 GAAGA GAAGA(8/7)
631 TCTTC
MspA1I 64 CAGCTG C[AC]G^C[GT]G
79 CCGCTG
MvnI 51 CGCG CG^CG
693 CGCG
SfaNI 276 GATGC GCATC(5/9)
770 GATGC
StyI 373 CCTTGG C^C[AT][AT]GG
515 CCATGG
TaqI 344 TCGA T^CGA
501 TCGA
TthHB8I 344 TCGA T^CGA
501 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AciI AvaII BbvI BcnI BsaJI
BsiYI BslI BsmFI BsrI BssKI
Cac8I CfoI Cfr13I DpnII DraII
EcoO109I Fnu4HI HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI MaeIII
MboI MnlI MslI MspI MwoI
NciI NdeII NlaIII NlaIV PleI
Sau3AI Sau96I ScrFI TseI Tsp509I
TspRI