(Circular) (Six-base) MAPSORT of: pFN21AB5204.seq Check: 5144 from: 1 to: 5512 pFN21AB5204 5512 bp With 182 enzymes: SgfI * October 20, 2009 11:00 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3322 278 Size: 53 83 186 936 1786 2468 Fragments arranged by size: 2468 1786 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2855 1937 Size: 918 4594 Acc65I G'GTAC_C Cuts at: 2833 2833 Size: 5512 AclI AA'CG_TT Cuts at: 1563 3639 4012 1563 Size: 2076 373 3063 Fragments arranged by size: 3063 2076 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3575 4959 5227 1632 Size: 1943 1384 268 1917 Fragments arranged by size: 1943 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5315 AflIII A'CryG_T Cuts at: 1203 4411 5388 1203 Size: 3208 977 1327 Fragments arranged by size: 3208 1327 977 AgeI A'CCGG_T Cuts at: 4343 4343 Size: 5512 AhdI GACnn_n'nnGTC Cuts at: 2356 4241 2356 Size: 1885 3627 AleI CACnn'nnGTG Cuts at: 1166 2184 1166 Size: 1018 4494 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5512 AlwNI CAG_nnn'CTG Cuts at: 2071 2086 3255 4827 2071 Size: 15 1169 1572 2756 Fragments arranged by size: 2756 1572 1169 15 ApaI G_GGCC'C Cuts at: 2484 2484 Size: 5512 ApaLI G'TGCA_C Cuts at: 3569 4725 3569 Size: 1156 4356 ApoI r'AATT_y Cuts at: 1493 2129 2492 2821 3047 3199 5308 1493 Size: 636 363 329 226 152 2109 1697 Fragments arranged by size: 2109 1697 636 363 329 226 152 AseI AT'TA_AT Cuts at: 160 1032 4066 160 Size: 872 3034 1606 Fragments arranged by size: 3034 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5512 AvaI C'yCGr_G Cuts at: 1870 1943 2285 2354 2837 1870 Size: 73 342 69 483 4545 Fragments arranged by size: 4545 483 342 73 69 BamHI G'GATC_C Cuts at: 2842 3209 2842 Size: 367 5145 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2421 2833 4288 618 Size: 325 217 627 634 412 1455 1842 Fragments arranged by size: 1842 1455 634 627 412 325 217 BanII G_rGCy'C Cuts at: 1391 2484 2831 1391 Size: 1093 347 4072 Fragments arranged by size: 4072 1093 347 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2201 928 Size: 971 302 4239 Fragments arranged by size: 4239 971 302 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3703 3737 1903 Size: 34 50 34 1682 34 3678 Fragments arranged by size: 3678 1682 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3404 4614 3404 Size: 1210 4302 BclI T'GATC_A Cuts at: 1548 1548 Size: 5512 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4123 136 Size: 107 122 71 631 605 2451 1525 Fragments arranged by size: 2451 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5507 5507 Size: 5512 BlpI GC'TnA_GC Cuts at: 2929 2929 Size: 5512 Bme1580I G_kGCm'C Cuts at: 1790 2484 2790 3573 4729 1790 Size: 694 306 783 1156 2573 Fragments arranged by size: 2573 1156 783 694 306 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5512 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3243 4201 442 Size: 416 53 83 399 24 1826 958 1753 Fragments arranged by size: 1826 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5512 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2500 2500 Size: 5512 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2550 2744 4172 1239 Size: 1311 194 1428 2579 Fragments arranged by size: 2579 1428 1311 194 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3637 4502 4800 3637 Size: 865 298 4349 Fragments arranged by size: 4349 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4175 882 Size: 617 2676 2219 Fragments arranged by size: 2676 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3303 493 Size: 711 2099 2702 Fragments arranged by size: 2702 2099 711 BsaBI GATnn'nnATC Cuts at: 5506 5506 Size: 5512 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3319 3701 4336 275 Size: 53 83 186 936 1786 382 635 1451 Fragments arranged by size: 1786 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3945 4343 4617 4764 5175 3945 Size: 398 274 147 411 4282 Fragments arranged by size: 4282 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2285 2315 2548 2578 1178 Size: 30 165 30 882 30 233 30 4112 Fragments arranged by size: 4112 882 233 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5512 BseYI C'CCAG_C Cuts at: 2071 4715 5211 2071 Size: 2644 496 2372 Fragments arranged by size: 2644 2372 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2327 2338 1163 Size: 573 591 11 4337 Fragments arranged by size: 4337 591 573 11 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2962 3723 4751 1540 Size: 275 186 961 761 1028 2301 Fragments arranged by size: 2301 1028 961 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3573 3658 4729 3573 Size: 85 1071 4356 Fragments arranged by size: 4356 1071 85 BsmI GAATG_Cn' Cuts at: 3023 3116 3023 Size: 93 5419 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2484 2790 2831 3573 3658 4729 1391 Size: 399 694 306 41 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 694 399 306 85 41 BspEI T'CCGG_A Cuts at: 5175 5175 Size: 5512 BspHI T'CATG_A Cuts at: 3401 5131 3401 Size: 1730 3782 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2653 2719 2867 844 Size: 1809 66 148 3489 Fragments arranged by size: 3489 1809 148 66 BsrBI CCG'CTC Cuts at: 1394 1823 3399 1394 Size: 429 1576 3507 Fragments arranged by size: 3507 1576 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4007 4181 65 Size: 1183 2759 174 1396 Fragments arranged by size: 2759 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2424 4156 4343 1700 Size: 110 614 1732 187 2869 Fragments arranged by size: 2869 1732 614 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5512 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5512 BssSI C'ACGA_G Cuts at: 2459 2485 3572 4584 2459 Size: 26 1087 1012 3387 Fragments arranged by size: 3387 1087 1012 26 BstAPI GCAn_nnn'nTGC Cuts at: 794 2086 794 Size: 1292 4220 BstBI TT'CG_AA Cuts at: 4316 4316 Size: 5512 BstEII G'GTnAC_C Cuts at: 1184 2731 1184 Size: 1547 3965 BstXI CCAn_nnnn'nTGG Cuts at: 2186 2186 Size: 5512 BstYI r'GATC_y Cuts at: 1972 2842 3209 3594 3611 5063 5149 5161 5507 Size: 870 367 385 17 1452 86 12 346 Cuts at: 5507 1972 Size: 1977 Fragments arranged by size: 1977 1452 870 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2389 2520 513 Size: 552 1324 131 3505 Fragments arranged by size: 3505 1324 552 131 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2002 487 Size: 683 56 776 3997 Fragments arranged by size: 3997 776 683 56 BtsI GCAGTG_nn' Cuts at: 2543 3028 3822 3842 2543 Size: 485 794 20 4213 Fragments arranged by size: 4213 794 485 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5512 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5477 DraI TTT'AAA Cuts at: 2816 2816 Size: 5512 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4519 809 Size: 507 3203 1802 Fragments arranged by size: 3203 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2717 2959 3848 Size: 54 1425 50 275 18 887 242 889 Cuts at: 3848 5477 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 887 275 242 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2959 1537 Size: 275 1147 4090 Fragments arranged by size: 4090 1147 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2641 3442 1353 Size: 1288 801 3423 Fragments arranged by size: 3423 1288 801 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2577 4099 4473 4619 387 Size: 1064 138 988 1522 374 146 1280 Fragments arranged by size: 1522 1280 1064 988 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2550 2744 3575 4172 4959 5227 1239 Size: 393 918 194 831 597 787 268 1524 Fragments arranged by size: 1524 918 831 787 597 393 268 194 EcoO109I rG'GnC_Cy Cuts at: 2480 2481 2711 2480 Size: 1 230 5281 Fragments arranged by size: 5281 230 1 EcoRI G'AATT_C Cuts at: 2129 2821 3199 2129 Size: 692 378 4442 Fragments arranged by size: 4442 692 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2062 2062 Size: 5512 FspI TGC'GCA Cuts at: 4018 4018 Size: 5512 HaeII r_GCGC'y Cuts at: 1678 2118 4659 1678 Size: 440 2541 2531 Fragments arranged by size: 2541 2531 440 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2342 2374 2548 2580 4153 Size: 32 135 32 770 32 174 32 1573 Cuts at: 4153 4185 4227 4259 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1573 770 174 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2622 2856 3102 669 Size: 988 281 684 234 246 3079 Fragments arranged by size: 3079 988 684 281 246 234 HindIII A'AGCT_T Cuts at: 748 748 Size: 5512 HpaI GTT'AAC Cuts at: 2622 3102 2622 Size: 480 5032 KpnI G_GTAC'C Cuts at: 2837 2837 Size: 5512 MfeI C'AATT_G Cuts at: 3111 3111 Size: 5512 MluI A'CGCG_T Cuts at: 5388 5388 Size: 5512 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4626 4810 1305 Size: 3321 184 2007 Fragments arranged by size: 3321 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3690 Fragments arranged by size: 3690 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2184 2265 2447 3470 3829 3988 518 Size: 648 1018 81 182 1023 359 159 2042 Fragments arranged by size: 2042 1023 1018 648 359 182 159 81 MspA1I CmG'CkG Cuts at: 1606 2071 2086 2928 3605 4753 4998 1606 Size: 465 15 842 677 1148 245 2120 Fragments arranged by size: 2120 1148 842 677 465 245 15 NcoI C'CATG_G Cuts at: 513 1065 2520 513 Size: 552 1455 3505 Fragments arranged by size: 3505 1455 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5512 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5512 NotI GC'GGCC_GC Cuts at: 2959 2959 Size: 5512 NspI r_CATG'y Cuts at: 1132 1229 2167 2870 4415 1132 Size: 97 938 703 1545 2229 Fragments arranged by size: 2229 1545 938 703 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5512 PciI A'CATG_T Cuts at: 4411 4411 Size: 5512 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5512 PmeI GTTT'AAAC Cuts at: 2816 2816 Size: 5512 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3581 3613 5094 5126 3581 Size: 32 1481 32 3967 Fragments arranged by size: 3967 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2711 2711 Size: 5512 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5512 PsiI TTA'TAA Cuts at: 3082 3082 Size: 5512 PspOMI G'GGCC_C Cuts at: 2480 2480 Size: 5512 PstI C_TGCA'G Cuts at: 830 2420 2864 830 Size: 1590 444 3478 Fragments arranged by size: 3478 1590 444 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5512 PvuII CAG'CTG Cuts at: 2071 2071 Size: 5512 SalI G'TCGA_C Cuts at: 1936 2854 1936 Size: 918 4594 SanDI GG'GwC_CC Cuts at: 2711 2711 Size: 5512 SbfI CC_TGCA'GG Cuts at: 2864 2864 Size: 5512 ScaI AGT'ACT Cuts at: 3760 5472 3760 Size: 1712 3800 SfcI C'TryA_G Cuts at: 826 1045 2416 2860 3995 4676 4867 826 Size: 219 1371 444 1135 681 191 1471 Fragments arranged by size: 1471 1371 1135 681 444 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5512 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5512 SmaI CCC'GGG Cuts at: 1872 2839 1872 Size: 967 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3616 4517 4779 820 Size: 197 926 1673 901 262 1553 Fragments arranged by size: 1673 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3303 493 Size: 2810 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5512 SphI G_CATG'C Cuts at: 1132 2870 1132 Size: 1738 3774 SspI AAT'ATT Cuts at: 5 52 3436 5 Size: 47 3384 2081 Fragments arranged by size: 3384 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5512 StyI C'CwwG_G Cuts at: 513 1065 2378 2520 2951 513 Size: 552 1313 142 431 3074 Fragments arranged by size: 3074 1313 552 431 142 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3539 3556 3709 3894 4339 325 Size: 1255 1959 17 153 185 445 1498 Fragments arranged by size: 1959 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3758 5470 96 Size: 274 80 33 51 1443 1781 1712 138 Fragments arranged by size: 1781 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5472 5504 5472 Size: 32 5480 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5512 XbaI T'CTAG_A Cuts at: 2848 2848 Size: 5512 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2788 2788 Size: 5512 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5512 XmaI C'CCGG_G Cuts at: 1870 2837 1870 Size: 967 4545 XmnI GAAnn'nnTTC Cuts at: 3641 3641 Size: 5512 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3320 276 Size: 53 83 186 936 1786 2468 Fragments arranged by size: 2468 1786 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI