Restriction map for pFN21AE3525
 (Linear) (Six-base) MAPSORT of: KIEE3525.seq  Check: 8967  from: 1  to: 747



KIEE3525.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 16:36  ..

AflII C'TTAA_G

Cuts at:      0      45     747 
   Size:         45     702
 
BamHI G'GATC_C

Cuts at:      0     126     747 
   Size:        126     621
 
BanII G_rGCy'C

Cuts at:      0     285     747 
   Size:        285     462
 
BbvCI CC'TCA_GC

Cuts at:      0     182     747 
   Size:        182     565
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     574     747 
   Size:        574     173
 
Bpu10I CC'TnA_GC

Cuts at:      0     182     747 
   Size:        182     565
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     604     613     688     747 
   Size:        604       9      75      59

  Fragments arranged by size: 

                 604      75      59       9
 
Bsp1286I G_dGCh'C

Cuts at:      0     285     747 
   Size:        285     462
 
BstYI r'GATC_y

Cuts at:      0     126     747 
   Size:        126     621
 
BtgI C'CryG_G

Cuts at:      0     176     197     747 
   Size:        176      21     550

  Fragments arranged by size: 

                 550     176      21
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     620     655     747 
   Size:        620      35      92

  Fragments arranged by size: 

                 620      92      35
 
EarI CTCTTCn'nnn_

Cuts at:      0     590     747 
   Size:        590     157
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     329     747 
   Size:        329     418
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     574     747 
   Size:        574     173
 
EcoO109I rG'GnC_Cy

Cuts at:      0     549     618     747 
   Size:        549      69     129

  Fragments arranged by size: 

                 549     129      69
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     539     747 
   Size:        539     208
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     391     423     747 
   Size:        391      32     324

  Fragments arranged by size: 

                 391     324      32
 
HindIII A'AGCT_T

Cuts at:      0      48     100     747 
   Size:         48      52     647

  Fragments arranged by size: 

                 647      52      48
 
MfeI C'AATT_G

Cuts at:      0     218     747 
   Size:        218     529
 
NcoI C'CATG_G

Cuts at:      0     176     197     747 
   Size:        176      21     550

  Fragments arranged by size: 

                 550     176      21
 
PpuMI rG'GwC_Cy

Cuts at:      0     549     618     747 
   Size:        549      69     129

  Fragments arranged by size: 

                 549     129      69
 
PsiI TTA'TAA

Cuts at:      0     718     747 
   Size:        718      29
 
SfcI C'TryA_G

Cuts at:      0     251     747 
   Size:        251     496
 
SmlI C'TyrA_G

Cuts at:      0      45     747 
   Size:         45     702
 
StyI C'CwwG_G

Cuts at:      0     176     197     747 
   Size:        176      21     550

  Fragments arranged by size: 

                 550     176      21
 
XbaI T'CTAG_A

Cuts at:      0     529     747 
   Size:        529     218
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     493     747 
   Size:        493     254
 

 Enzymes that do cut:

    AflII    BamHI    BanII    BbvCI     BpmI   Bpu10I    BseRI Bsp1286I
    BstYI     BtgI    CspCI     EarI     EciI  Eco57MI EcoO109I     FalI
    Hin4I  HindIII     MfeI     NcoI    PpuMI     PsiI     SfcI     SmlI
     StyI     XbaI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI     BanI
     BbeI     BbsI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI     DraI   DraIII
     DrdI     EaeI     EagI  EcoICRI    EcoNI    EcoRI    EcoRV     FseI
     FspI    FspAI    HaeII   HincII     HpaI     KasI     KpnI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII     TatI     TstI  Tth111I     XhoI     XmaI     XmnI
     ZraI
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