(Circular) (Six-base) MAPSORT of: pFN21AB5139.seq Check: 367 from: 1 to: 6406 pFN21AB5139 6406 bp With 182 enzymes: SgfI * October 20, 2009 11:00 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3224 3224 Size: 6406 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4216 278 Size: 53 83 186 936 2680 2468 Fragments arranged by size: 2680 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2700 3749 1937 Size: 763 1049 4594 Fragments arranged by size: 4594 1049 763 Acc65I G'GTAC_C Cuts at: 3043 3727 3043 Size: 684 5722 AclI AA'CG_TT Cuts at: 1563 4533 4906 1563 Size: 2970 373 3063 Fragments arranged by size: 3063 2970 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3475 3559 4469 5853 6121 1632 Size: 1843 84 910 1384 268 1917 Fragments arranged by size: 1917 1843 1384 910 268 84 AfeI AGC'GCT Cuts at: 2735 2819 3167 2735 Size: 84 348 5974 Fragments arranged by size: 5974 348 84 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6209 AflIII A'CryG_T Cuts at: 1203 5305 6282 1203 Size: 4102 977 1327 Fragments arranged by size: 4102 1327 977 AgeI A'CCGG_T Cuts at: 5237 5237 Size: 6406 AhdI GACnn_n'nnGTC Cuts at: 5135 5135 Size: 6406 AleI CACnn'nnGTG Cuts at: 1166 2135 2364 1166 Size: 969 229 5208 Fragments arranged by size: 5208 969 229 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6406 AlwNI CAG_nnn'CTG Cuts at: 2095 4149 5721 2095 Size: 2054 1572 2780 Fragments arranged by size: 2780 2054 1572 ApaI G_GGCC'C Cuts at: 2030 2073 2173 2416 2646 3082 3181 2030 Size: 43 100 243 230 436 99 5255 Fragments arranged by size: 5255 436 243 230 100 99 43 ApaLI G'TGCA_C Cuts at: 2597 2774 3275 4463 5619 2597 Size: 177 501 1188 1156 3384 Fragments arranged by size: 3384 1188 1156 501 177 ApoI r'AATT_y Cuts at: 1493 3715 3941 4093 6202 1493 Size: 2222 226 152 2109 1697 Fragments arranged by size: 2222 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4960 160 Size: 872 3928 1606 Fragments arranged by size: 3928 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6406 AvaI C'yCGr_G Cuts at: 1870 1943 2804 2983 3731 1870 Size: 73 861 179 748 4545 Fragments arranged by size: 4545 861 748 179 73 BamHI G'GATC_C Cuts at: 3736 4103 3736 Size: 367 6039 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2150 2238 2502 3043 3626 Size: 325 217 627 363 88 264 541 583 Cuts at: 3626 3727 5182 618 Size: 101 1455 1842 Fragments arranged by size: 1842 1455 627 583 541 363 325 264 217 101 88 BanII G_rGCy'C Cuts at: 1391 2030 2073 2173 2416 2491 2646 2993 3033 Size: 639 43 100 243 75 155 347 40 Cuts at: 3033 3082 3181 3725 1391 Size: 49 99 544 4072 Fragments arranged by size: 4072 639 544 347 243 155 100 99 75 49 43 40 BbeI G_GCGC'C Cuts at: 3630 3630 Size: 6406 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3254 928 Size: 971 1355 4080 Fragments arranged by size: 4080 1355 971 BbvCI CC'TCA_GC Cuts at: 2117 2607 2117 Size: 490 5916 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4597 4631 1903 Size: 34 50 34 2576 34 3678 Fragments arranged by size: 3678 2576 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4298 5508 4298 Size: 1210 5196 BclI T'GATC_A Cuts at: 1548 1548 Size: 6406 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2522 5017 136 Size: 107 122 71 631 605 850 2495 1525 Fragments arranged by size: 2495 1525 850 631 605 122 107 71 BglII A'GATC_T Cuts at: 6401 6401 Size: 6406 BlpI GC'TnA_GC Cuts at: 3823 3823 Size: 6406 Bme1580I G_kGCm'C Cuts at: 1790 2030 2073 2155 2173 2185 2416 2601 2646 Size: 240 43 82 18 12 231 185 45 Cuts at: 2646 2778 2804 3082 3181 3279 4467 5623 1790 Size: 132 26 278 99 98 1188 1156 2573 Fragments arranged by size: 2573 1188 1156 278 240 231 185 132 99 98 82 45 43 26 18 12 BmgBI CAC'GTC Cuts at: 1318 2133 1318 Size: 815 5591 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2137 2560 4137 Size: 416 53 83 399 24 720 423 1577 Cuts at: 4137 5095 442 Size: 958 1753 Fragments arranged by size: 1753 1577 958 720 423 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6406 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2636 3182 5066 1239 Size: 1397 546 1884 2579 Fragments arranged by size: 2579 1884 1397 546 Bpu10I CC'TnA_GC Cuts at: 2117 2607 2117 Size: 490 5916 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4531 5396 5694 4531 Size: 865 298 5243 Fragments arranged by size: 5243 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5069 882 Size: 617 3570 2219 Fragments arranged by size: 3570 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2602 4197 493 Size: 711 1398 1595 2702 Fragments arranged by size: 2702 1595 1398 711 BsaBI GATnn'nnATC Cuts at: 6400 6400 Size: 6406 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3627 4213 4595 5230 Size: 53 83 186 936 2094 586 382 635 Cuts at: 5230 275 Size: 1451 Fragments arranged by size: 2094 1451 936 635 586 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4839 5237 5511 5658 6069 4839 Size: 398 274 147 411 5176 Fragments arranged by size: 5176 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2682 2712 1178 Size: 30 165 30 1279 30 4872 Fragments arranged by size: 4872 1279 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2633 3320 3683 1186 Size: 1447 687 363 3909 Fragments arranged by size: 3909 1447 687 363 BseYI C'CCAG_C Cuts at: 2049 2208 2385 2994 5609 6105 2049 Size: 159 177 609 2615 496 2350 Fragments arranged by size: 2615 2350 609 496 177 159 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2294 1163 Size: 573 558 5275 Fragments arranged by size: 5275 573 558 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2704 2899 3027 3108 3316 3373 Size: 275 186 703 195 128 81 208 57 Cuts at: 3373 3568 3856 4617 5645 1540 Size: 195 288 761 1028 2301 Fragments arranged by size: 2301 1028 761 703 288 275 208 195 195 186 128 81 57 BsiHKAI G_wGCw'C Cuts at: 2491 2601 2778 3093 3279 4467 4552 5623 2491 Size: 110 177 315 186 1188 85 1071 3274 Fragments arranged by size: 3274 1188 1071 315 186 177 110 85 BsmI GAATG_Cn' Cuts at: 3917 4010 3917 Size: 93 6313 BsmBI CGTCTCn'nnnn_ Cuts at: 2452 2452 Size: 6406 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2030 2073 2155 2173 2185 2416 2491 Size: 399 240 43 82 18 12 231 75 Cuts at: 2491 2601 2646 2778 2804 2993 3033 3082 3093 Size: 110 45 132 26 189 40 49 11 Cuts at: 3093 3181 3279 3725 4467 4552 5623 1391 Size: 88 98 446 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 446 399 240 231 189 132 110 98 88 85 82 75 49 45 43 40 26 18 12 11 BspEI T'CCGG_A Cuts at: 6069 6069 Size: 6406 BspHI T'CATG_A Cuts at: 4295 6025 4295 Size: 1730 4676 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2931 3224 3761 844 Size: 2087 293 537 3489 Fragments arranged by size: 3489 2087 537 293 BsrBI CCG'CTC Cuts at: 1394 1823 2788 3205 3289 3541 4293 1394 Size: 429 965 417 84 252 752 3507 Fragments arranged by size: 3507 965 752 429 417 252 84 BsrDI GCAATG_nn' Cuts at: 65 1248 4901 5075 65 Size: 1183 3653 174 1396 Fragments arranged by size: 3653 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2235 2850 2864 3005 3055 3435 3449 Size: 110 425 615 14 141 50 380 14 Cuts at: 3449 3519 5050 5237 1700 Size: 70 1531 187 2869 Fragments arranged by size: 2869 1531 615 425 380 187 141 110 70 50 14 14 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6406 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6406 BssSI C'ACGA_G Cuts at: 4466 5478 4466 Size: 1012 5394 BstAPI GCAn_nnn'nTGC Cuts at: 794 2892 794 Size: 2098 4308 BstBI TT'CG_AA Cuts at: 5210 5210 Size: 6406 BstEII G'GTnAC_C Cuts at: 1184 2668 1184 Size: 1484 4922 BstXI CCAn_nnnn'nTGG Cuts at: 2044 2366 2638 2044 Size: 322 272 5812 Fragments arranged by size: 5812 322 272 BstYI r'GATC_y Cuts at: 1972 3736 4103 4488 4505 5957 6043 6055 6401 Size: 1764 367 385 17 1452 86 12 346 Cuts at: 6401 1972 Size: 1977 Fragments arranged by size: 1977 1764 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2037 2110 2664 3021 513 Size: 552 972 73 554 357 3898 Fragments arranged by size: 3898 972 554 552 357 73 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2159 2422 487 Size: 683 56 933 263 4471 Fragments arranged by size: 4471 933 683 263 56 BtsI GCAGTG_nn' Cuts at: 2306 3922 4716 4736 2306 Size: 1616 794 20 3976 Fragments arranged by size: 3976 1616 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6406 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6371 DraI TTT'AAA Cuts at: 3710 3710 Size: 6406 DraIII CAC_nnn'GTG Cuts at: 3259 3259 Size: 6406 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5413 809 Size: 507 4097 1802 Fragments arranged by size: 4097 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2034 2275 2551 Size: 54 1425 50 275 18 204 241 276 Cuts at: 2551 2896 3024 3313 3649 3853 4742 6371 8 Size: 345 128 289 336 204 889 1629 43 Fragments arranged by size: 1629 1425 889 345 336 289 276 275 241 204 204 128 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2896 3024 3313 3853 1537 Size: 275 1084 128 289 540 4090 Fragments arranged by size: 4090 1084 540 289 275 128 EarI CTCTTCn'nnn_ Cuts at: 1353 4336 1353 Size: 2983 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3398 4993 5367 5513 387 Size: 1064 138 1809 1595 374 146 1280 Fragments arranged by size: 1809 1595 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2489 2489 Size: 6406 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2636 3182 3475 3559 4469 5066 5853 Size: 393 1004 546 293 84 910 597 787 Cuts at: 5853 6121 1239 Size: 268 1524 Fragments arranged by size: 1524 1004 910 787 597 546 393 293 268 84 EcoNI CCTnn'n_nnAGG Cuts at: 2099 2618 2099 Size: 519 5887 EcoO109I rG'GnC_Cy Cuts at: 2026 2027 2070 2169 2611 2642 2643 3078 3079 Size: 1 43 99 442 31 1 435 1 Cuts at: 3079 3100 2026 Size: 21 5332 Fragments arranged by size: 5332 442 435 99 43 31 21 1 1 1 EcoRI G'AATT_C Cuts at: 3715 4093 3715 Size: 378 6028 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2770 2770 Size: 6406 FspI TGC'GCA Cuts at: 4912 4912 Size: 6406 HaeII r_GCGC'y Cuts at: 1678 2737 2821 3169 3630 5553 1678 Size: 1059 84 348 461 1923 2531 Fragments arranged by size: 2531 1923 1059 461 348 84 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2682 2714 5047 5079 5121 Size: 32 135 32 1110 32 2333 32 42 Cuts at: 5121 5153 1373 Size: 32 2626 Fragments arranged by size: 2626 2333 1110 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2701 3750 3996 669 Size: 988 281 763 1049 246 3079 Fragments arranged by size: 3079 1049 988 763 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6406 HpaI GTT'AAC Cuts at: 3996 3996 Size: 6406 KasI G'GCGC_C Cuts at: 3626 3626 Size: 6406 KpnI G_GTAC'C Cuts at: 3047 3731 3047 Size: 684 5722 MfeI C'AATT_G Cuts at: 4005 4005 Size: 6406 MluI A'CGCG_T Cuts at: 6282 6282 Size: 6406 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3528 3612 5520 5704 1305 Size: 2223 84 1908 184 2007 Fragments arranged by size: 2223 2007 1908 184 84 MscI TGG'CCA Cuts at: 10 64 1489 1832 2036 3651 10 Size: 54 1425 343 204 1615 2765 Fragments arranged by size: 2765 1615 1425 343 204 54 MslI CAynn'nnrTG Cuts at: 518 1166 2135 2178 2364 4364 4723 4882 518 Size: 648 969 43 186 2000 359 159 2042 Fragments arranged by size: 2042 2000 969 648 359 186 159 43 MspA1I CmG'CkG Cuts at: 1606 2112 2423 2666 2998 3023 3132 3187 3822 Size: 506 311 243 332 25 109 55 635 Cuts at: 3822 4499 5647 5892 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 635 506 332 311 245 243 109 55 25 NaeI GCC'GGC Cuts at: 3007 3007 Size: 6406 NarI GG'CG_CC Cuts at: 3627 3627 Size: 6406 NcoI C'CATG_G Cuts at: 513 1065 2037 513 Size: 552 972 4882 Fragments arranged by size: 4882 972 552 NdeI CA'TA_TG Cuts at: 387 2537 387 Size: 2150 4256 NgoMIV G'CCGG_C Cuts at: 3005 3005 Size: 6406 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6406 NotI GC'GGCC_GC Cuts at: 2896 3313 3853 2896 Size: 417 540 5449 Fragments arranged by size: 5449 540 417 NspI r_CATG'y Cuts at: 1132 1229 3764 5309 1132 Size: 97 2535 1545 2229 Fragments arranged by size: 2535 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2095 2652 3328 1348 Size: 747 557 676 4426 Fragments arranged by size: 4426 747 676 557 PciI A'CATG_T Cuts at: 5305 5305 Size: 6406 PflMI CCAn_nnn'nTGG Cuts at: 1108 2095 1108 Size: 987 5419 PfoI T'CCnGG_A Cuts at: 3618 3618 Size: 6406 PmeI GTTT'AAAC Cuts at: 3710 3710 Size: 6406 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4475 4507 5988 6020 4475 Size: 32 1481 32 4861 Fragments arranged by size: 4861 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3100 3100 Size: 6406 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6406 PsiI TTA'TAA Cuts at: 3976 3976 Size: 6406 PspOMI G'GGCC_C Cuts at: 2026 2069 2169 2412 2642 3078 3177 2026 Size: 43 100 243 230 436 99 5255 Fragments arranged by size: 5255 436 243 230 100 99 43 PstI C_TGCA'G Cuts at: 830 3758 830 Size: 2928 3478 PvuI CG_AT'CG Cuts at: 2001 3108 3373 2001 Size: 1107 265 5034 Fragments arranged by size: 5034 1107 265 PvuII CAG'CTG Cuts at: 2423 2998 3132 2423 Size: 575 134 5697 Fragments arranged by size: 5697 575 134 SacI G_AGCT'C Cuts at: 2491 2491 Size: 6406 SacII CC_GC'GG Cuts at: 2113 2667 3024 2113 Size: 554 357 5495 Fragments arranged by size: 5495 554 357 SalI G'TCGA_C Cuts at: 1936 2699 3748 1936 Size: 763 1049 4594 Fragments arranged by size: 4594 1049 763 SbfI CC_TGCA'GG Cuts at: 3758 3758 Size: 6406 ScaI AGT'ACT Cuts at: 4654 6366 4654 Size: 1712 4694 SexAI A'CCwGG_T Cuts at: 3255 3507 3255 Size: 252 6154 SfcI C'TryA_G Cuts at: 826 1045 3127 3754 4889 5570 5761 826 Size: 219 2082 627 1135 681 191 1471 Fragments arranged by size: 2082 1471 1135 681 627 219 191 SfoI GGC'GCC Cuts at: 3628 3628 Size: 6406 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6406 SgrAI Cr'CCGG_yG Cuts at: 1700 2235 2850 2864 3435 3449 3519 1700 Size: 535 615 14 571 14 70 4587 Fragments arranged by size: 4587 615 571 535 70 14 14 SmaI CCC'GGG Cuts at: 1872 2985 3733 1872 Size: 1113 748 4545 Fragments arranged by size: 4545 1113 748 SmlI C'TyrA_G Cuts at: 820 1017 1943 4510 5411 5673 820 Size: 197 926 2567 901 262 1553 Fragments arranged by size: 2567 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4197 493 Size: 3704 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6406 SphI G_CATG'C Cuts at: 1132 3764 1132 Size: 2632 3774 SspI AAT'ATT Cuts at: 5 52 4330 5 Size: 47 4278 2081 Fragments arranged by size: 4278 2081 47 StuI AGG'CCT Cuts at: 1517 3404 1517 Size: 1887 4519 StyI C'CwwG_G Cuts at: 513 1065 2037 2631 3845 513 Size: 552 972 594 1214 3074 Fragments arranged by size: 3074 1214 972 594 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2200 2377 3108 3614 4433 4450 4603 Size: 1255 620 177 731 506 819 17 153 Cuts at: 4603 4788 5233 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 819 731 620 506 445 185 177 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4652 6364 96 Size: 274 80 33 51 1443 2675 1712 138 Fragments arranged by size: 2675 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2936 2968 6366 6398 2936 Size: 32 3398 32 2944 Fragments arranged by size: 3398 2944 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6406 XbaI T'CTAG_A Cuts at: 3742 3742 Size: 6406 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2341 2341 Size: 6406 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6406 XmaI C'CCGG_G Cuts at: 1870 2983 3731 1870 Size: 1113 748 4545 Fragments arranged by size: 4545 1113 748 XmnI GAAnn'nnTTC Cuts at: 3236 4535 3236 Size: 1299 5107 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4214 276 Size: 53 83 186 936 2680 2468 Fragments arranged by size: 2680 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I Bsu36I EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SanDI SapI SfiI SrfI SwaI