Restriction map for
pFN21AB5023
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# NO CUT ENZYMES ( 171 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspI AvaI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbvCI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsmFI BsoBI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII Bst1107I BstBI BstEII
BstF5I BstPI BstUI BstYI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraII DraIII DrdI EagI
EarI EclXI Eco47III Eco52I Eco81I
EcoO109I EcoO65I EcoRI EcoRV FauI
FbaI FokI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HindIII HpaI HpaII KasI KpnI
Ksp632I KspI MaeII MamI MfeI
MflI MluI MluNI MroI MscI
MspA1I MspI MunI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshBI Psp1406I PstI
PvuI PvuII RsrII SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI Tth111I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AhdI 53 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 420 GATCC GGATC(4/5)
AlwNI 426 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspEI 53 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 413 GAGCTC G[AT]GC[AT]^C
BbsI 704 GTCTTC GAAGAC(2/6)
BcgI 781 GCAAAGGAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpmI 443 CTGGAG CTGGAG(16/14)
BpuAI 704 GTCTTC GAAGAC(2/6)
BseRI 476 GAGGAG GAGGAG(10/8)
BsiHKAI 413 GAGCTC G[AT]GC[AT]^C
BsiYI 889 CCTGTGATAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 889 CCTGTGATAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI 389 GCATTC GAATGC(1/-1)
BspHI 933 TCATGA T^CATGA
BssKI 205 CCTGG ^CC[ACGT]GG
BssSI 767 CACGAG CACGAG(-5/-1)
BstAPI 310 GCAGAGTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 205 CCTGG CC^[AT]GG
BstXI 126 CCATGCATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 493 GCATGC GC[ACGT]^[ACGT]GC
DraI 367 TTTAAA TTT^AAA
EaeI 923 TGGCCG [CT]^GGCC[GA]
Eam1105I 53 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 889 CCTGTGATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII 205 CCTGG ^CC[AT]GG
HaeIII 924 GGCC GG^CC
HincII 139 GTCAAC GT[CT]^[GA]AC
HindII 139 GTCAAC GT[CT]^[GA]AC
MvaI 205 CCTGG CC^[AT]GG
NcoI 776 CCATGG C^CATGG
NlaIV 577 GGAGCC GG[ACGT]^[ACGT]CC
PshAI 697 GACTTGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 933 TCATGA T^CATGA
SacI 413 GAGCTC GAGCT^C
ScrFI 205 CCTGG CC^[ACGT]GG
SfaNI 250 GCATC GCATC(5/9)
SmlI 699 CTTGAG C^T[CT][GA]AG
SphI 493 GCATGC GCATG^C
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# TWO CUT ENZYMES ( 22 enzymes) #
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AcsI 43 GAATTT [GA]^AATT[CT]
832 GAATTT
ApoI 43 GAATTT [GA]^AATT[CT]
832 GAATTT
AvaII 52 GGACC G^G[AT]CC
123 GGTCC
BanII 413 GAGCTC G[GA]GC[CT]^C
578 GAGCCC
BfaI 284 CTAG C^TAG
506 CTAG
BsmAI 151 GAGAC GTCTC(1/5)
770 GAGAC
Cfr13I 52 GGACC G^G[ACGT]CC
123 GGTCC
DsaI 146 CCGTGG C^C[GA][CT]GG
776 CCATGG
EcoT14I 776 CCATGG C^C[AT][AT]GG
824 CCAAGG
EcoT22I 128 ATGCAT ATGCA^T
248 ATGCAT
MaeI 284 CTAG C^TAG
506 CTAG
MboII 705 TCTTC GAAGA(8/7)
978 TCTTC
MslI 127 CATGCATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
949 CATGGTAGTG
NsiI 128 ATGCAT ATGCA^T
248 ATGCAT
NspI 130 GCATGT [GA]CATG^[CT]
493 GCATGC
PleI 702 GAGTC GAGTC(4/5)
787 GAGTC
Sau96I 52 GGACC G^G[ACGT]CC
123 GGTCC
StyI 776 CCATGG C^C[AT][AT]GG
824 CCAAGG
TaqI 411 TCGA T^CGA
790 TCGA
TfiI 93 GATTC G^A[AT]TC
975 GATTC
Tsp45I 269 GTGAC ^GT[GC]AC
711 GTCAC
TthHB8I 411 TCGA T^CGA
790 TCGA
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# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AfaI AluI BbvI BmyI BsaJI
Bsp1286I BsrI DdeI DpnII Fnu4HI
HinfI HphI ItaI MaeIII MboI
MnlI MseI MwoI NdeII NlaIII
RsaI Sau3AI Tru9I TseI Tsp509I
TspRI