Restriction map for pFN21AB5023
 (Circular) (Six-base) MAPSORT of: pFN21AB5023.seq  Check: 9408  from: 1  to: 5692



pFN21AB5023  5692 bp

 With 182 enzymes: SgfI * 

                        October 20, 2009 11:00  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3502     278 
   Size:         53      83     186     936    1966    2468

  Fragments arranged by size: 

                2468    1966     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3035    1937 
   Size:       1098    4594
 
Acc65I G'GTAC_C

Cuts at:   3013    3013 
   Size:       5692
 
AclI AA'CG_TT

Cuts at:   1563    3819    4192    1563 
   Size:       2256     373    3063

  Fragments arranged by size: 

                3063    2256     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3755    5139    5407    1632 
   Size:       2123    1384     268    1917

  Fragments arranged by size: 

                2123    1917    1384     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5495
 
AflIII A'CryG_T

Cuts at:   1203    4591    5568    1203 
   Size:       3388     977    1327

  Fragments arranged by size: 

                3388    1327     977
 
AgeI A'CCGG_T

Cuts at:   4523    4523 
   Size:       5692
 
AhdI GACnn_n'nnGTC

Cuts at:   2063    4421    2063 
   Size:       2358    3334
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5692
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5692
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2442    2474    2442 
   Size:         32    5660
 
AlwNI CAG_nnn'CTG

Cuts at:   2436    3435    5007    2436 
   Size:        999    1572    3121

  Fragments arranged by size: 

                3121    1572     999
 
ApaLI G'TGCA_C

Cuts at:   3749    4905    3749 
   Size:       1156    4536
 
ApoI r'AATT_y

Cuts at:   1493    2048    2837    3001    3227    3379    5488    1493 
   Size:        555     789     164     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     789     555     226     164     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4246     160 
   Size:        872    3214    1606

  Fragments arranged by size: 

                3214    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5692
 
AvaI C'yCGr_G

Cuts at:   1870    1943    3017    1870 
   Size:         73    1074    4545

  Fragments arranged by size: 

                4545    1074      73
 
BamHI G'GATC_C

Cuts at:   3022    3389    3022 
   Size:        367    5325
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    3013    4468     618 
   Size:        325     217     627    1226    1455    1842

  Fragments arranged by size: 

                1842    1455    1226     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2422    2587    3011    1391 
   Size:       1031     165     424    4072

  Fragments arranged by size: 

                4072    1031     424     165
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2702     928 
   Size:        971     803    3918

  Fragments arranged by size: 

                3918     971     803
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    2775    2809    3883    3917    1903 
   Size:         34     838      34    1074      34    3678

  Fragments arranged by size: 

                3678    1074     838      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3584    4794    3584 
   Size:       1210    4482
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5692
 
BfrBI ATG'CAT

Cuts at:   2135    2255    2135 
   Size:        120    5572
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4303     136 
   Size:        107     122      71     631     605    2631    1525

  Fragments arranged by size: 

                2631    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5687    5687 
   Size:       5692
 
BlpI GC'TnA_GC

Cuts at:   3109    3109 
   Size:       5692
 
Bme1580I G_kGCm'C

Cuts at:   1790    2540    3753    4909    1790 
   Size:        750    1213    1156    2573

  Fragments arranged by size: 

                2573    1213    1156     750
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5692
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3423    4381     442 
   Size:        416      53      83     399      24    2006     958    1753

  Fragments arranged by size: 

                2006    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5692
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2831    2831 
   Size:       5692
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2469    4352    1239 
   Size:       1230    1883    2579

  Fragments arranged by size: 

                2579    1883    1230
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2725    3817    4682    4980    2725 
   Size:       1092     865     298    3437

  Fragments arranged by size: 

                3437    1092     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4355     882 
   Size:        617    2856    2219

  Fragments arranged by size: 

                2856    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3483     493 
   Size:        711    2279    2702

  Fragments arranged by size: 

                2702    2279     711
 
BsaBI GATnn'nnATC

Cuts at:   5686    5686 
   Size:       5692
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3499    3881    4516     275 
   Size:         53      83     186     936    1966     382     635    1451

  Fragments arranged by size: 

                1966    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   4125    4523    4797    4944    5355    4125 
   Size:        398     274     147     411    4462

  Fragments arranged by size: 

                4462     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2442    2472    2715    2745    1178 
   Size:         30     165      30    1039      30     243      30    4125

  Fragments arranged by size: 

                4125    1039     243     165      30      30      30      30
            
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2496    1186 
   Size:       1310    4382
 
BseYI C'CCAG_C

Cuts at:   2225    4895    5391    2225 
   Size:       2670     496    2526

  Fragments arranged by size: 

                2670    2526     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    5119
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3142    3903    4931    1540 
   Size:        275     186    1141     761    1028    2301

  Fragments arranged by size: 

                2301    1141    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2422    3753    3838    4909    2422 
   Size:       1331      85    1071    3205

  Fragments arranged by size: 

                3205    1331    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2394    3203    3296    2394 
   Size:        809      93    4790

  Fragments arranged by size: 

                4790     809      93
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2422    2540    2587    3011    3753    3838    4909 
   Size:        399     632     118      47     424     742      85    1071

Cuts at:   4909    1391 
   Size:       2174

  Fragments arranged by size: 

                2174    1071     742     632     424     399     118      85
                  47
 
BspEI T'CCGG_A

Cuts at:   5355    5355 
   Size:       5692
 
BspHI T'CATG_A

Cuts at:   2938    3581    5311    2938 
   Size:        643    1730    3319

  Fragments arranged by size: 

                3319    1730     643
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    3047     844 
   Size:       2203    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3579    1394 
   Size:        429    1756    3507

  Fragments arranged by size: 

                3507    1756     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4187    4361      65 
   Size:       1183    2939     174    1396

  Fragments arranged by size: 

                2939    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4336    4523    1700 
   Size:        110    2526     187    2869

  Fragments arranged by size: 

                2869    2526     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5692
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5692
 
BssSI C'ACGA_G

Cuts at:   2772    3752    4764    2772 
   Size:        980    1012    3700

  Fragments arranged by size: 

                3700    1012     980
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2321     794 
   Size:       1527    4165
 
BstBI TT'CG_AA

Cuts at:   4496    4496 
   Size:       5692
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5692
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2138    2138 
   Size:       5692
 
BstYI r'GATC_y

Cuts at:   1972    3022    3389    3774    3791    5243    5329    5341    5687 
   Size:       1050     367     385      17    1452      86      12     346

Cuts at:   5687    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452    1050     385     367     346      86      17
                  12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2151    2781     513 
   Size:        552     939     147     630    3424

  Fragments arranged by size: 

                3424     939     630     552     147
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4953

  Fragments arranged by size: 

                4953     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2538    3208    4002    4022    2538 
   Size:        670     794      20    4208

  Fragments arranged by size: 

                4208     794     670      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5692
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5657
 
DraI TTT'AAA

Cuts at:   2374    2996    2374 
   Size:        622    5070
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4699     809 
   Size:        507    3383    1802

  Fragments arranged by size: 

                3383    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2928    3139    4028 
   Size:         54    1425      50     275      18    1098     211     889

Cuts at:   4028    5657       8 
   Size:       1629      43

  Fragments arranged by size: 

                1629    1425    1098     889     275     211      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3139    1537 
   Size:        275    1327    4090

  Fragments arranged by size: 

                4090    1327     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3622    1353 
   Size:       2269    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4279    4653    4799     387 
   Size:       1064     138    2690     374     146    1280

  Fragments arranged by size: 

                2690    1280    1064     374     146     138
 
EcoICRI GAG'CTC

Cuts at:   2420    2420 
   Size:       5692
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2469    3755    4352    5139    5407    1239 
   Size:        393     837    1286     597     787     268    1524

  Fragments arranged by size: 

                1524    1286     837     787     597     393     268
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2898    2898 
   Size:       5692
 
EcoRI G'AATT_C

Cuts at:   3001    3379    3001 
   Size:        378    5314
 
FspI TGC'GCA

Cuts at:   4198    4198 
   Size:       5692
 
HaeII r_GCGC'y

Cuts at:   1678    4839    1678 
   Size:       3161    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2049    2081    2240    2272    2373 
   Size:         32     135      32     477      32     159      32     101

Cuts at:   2373    2405    2409    2441    2890    2922    4333    4365    4407 
   Size:         32       4      32     449      32    1411      32      42

Cuts at:   4407    4439    1373 
   Size:         32    2626

  Fragments arranged by size: 

                2626    1411     477     449     159     135     101      42
                  32      32      32      32      32      32      32      32
                  32       4
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2146    3036    3282     669 
   Size:        988     281     208     890     246    3079

  Fragments arranged by size: 

                3079     988     890     281     246     208
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5692
 
HpaI GTT'AAC

Cuts at:   3282    3282 
   Size:       5692
 
KpnI G_GTAC'C

Cuts at:   3017    3017 
   Size:       5692
 
MfeI C'AATT_G

Cuts at:   3291    3291 
   Size:       5692
 
MluI A'CGCG_T

Cuts at:   5568    5568 
   Size:       5692
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2224    2467    4806    4990    1305 
   Size:        919     243    2339     184    2007

  Fragments arranged by size: 

                2339    2007     919     243     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3870

  Fragments arranged by size: 

                3870    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2136    2958    3650    4009    4168     518 
   Size:        648     970     822     692     359     159    2042

  Fragments arranged by size: 

                2042     970     822     692     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    3108    3785    4933    5178    1606 
   Size:       1502     677    1148     245    2120

  Fragments arranged by size: 

                2120    1502    1148     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2004    2781     513 
   Size:        552     939     777    3424

  Fragments arranged by size: 

                3424     939     777     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5692
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5692
 
NotI GC'GGCC_GC

Cuts at:   3139    3139 
   Size:       5692
 
NsiI A_TGCA'T

Cuts at:   2137    2257    2137 
   Size:        120    5572
 
NspI r_CATG'y

Cuts at:   1132    1229    2139    2502    3050    4595    1132 
   Size:         97     910     363     548    1545    2229

  Fragments arranged by size: 

                2229    1545     910     548     363      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5692
 
PciI A'CATG_T

Cuts at:   4591    4591 
   Size:       5692
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5692
 
PmeI GTTT'AAAC

Cuts at:   2996    2996 
   Size:       5692
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2442    2474    3761    3793    5274    5306    2442 
   Size:         32    1287      32    1481      32    2828

  Fragments arranged by size: 

                2828    1481    1287      32      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    2706    1613 
   Size:       1093    4599
 
PsiI TTA'TAA

Cuts at:   3262    3262 
   Size:       5692
 
PstI C_TGCA'G

Cuts at:    830    3044     830 
   Size:       2214    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5692
 
SacI G_AGCT'C

Cuts at:   2422    2422 
   Size:       5692
 
SalI G'TCGA_C

Cuts at:   1936    3034    1936 
   Size:       1098    4594
 
SbfI CC_TGCA'GG

Cuts at:   3044    3044 
   Size:       5692
 
ScaI AGT'ACT

Cuts at:   3940    5652    3940 
   Size:       1712    3980
 
SfcI C'TryA_G

Cuts at:    826    1045    3040    4175    4856    5047     826 
   Size:        219    1995    1135     681     191    1471

  Fragments arranged by size: 

                1995    1471    1135     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5692
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5692
 
SmaI CCC'GGG

Cuts at:   1872    3019    1872 
   Size:       1147    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2704    3796    4697    4959     820 
   Size:        197     926     761    1092     901     262    1553

  Fragments arranged by size: 

                1553    1092     926     901     761     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3483     493 
   Size:       2990    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5692
 
SphI G_CATG'C

Cuts at:   1132    2502    3050    1132 
   Size:       1370     548    3774

  Fragments arranged by size: 

                3774    1370     548
 
SspI AAT'ATT

Cuts at:      5      52    3616       5 
   Size:         47    3564    2081

  Fragments arranged by size: 

                3564    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5692
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2781    2829    3131     513 
   Size:        552     939     777      48     302    3074

  Fragments arranged by size: 

                3074     939     777     552     302      48
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3719    3736    3889    4074    4519     325 
   Size:       1255    2139      17     153     185     445    1498

  Fragments arranged by size: 

                2139    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3938    5650      96 
   Size:        274      80      33      51    1443    1961    1712     138

  Fragments arranged by size: 

                1961    1712    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2715    2747    5652    5684    2715 
   Size:         32    2905      32    2723

  Fragments arranged by size: 

                2905    2723      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5692
 
XbaI T'CTAG_A

Cuts at:   3028    3028 
   Size:       5692
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5692
 
XmaI C'CCGG_G

Cuts at:   1870    3017    1870 
   Size:       1147    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3821    3821 
   Size:       5692
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3500     276 
   Size:         53      83     186     936    1966    2468

  Fragments arranged by size: 

                2468    1966     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI    EcoRI     FspI    HaeII
    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NcoI     NdeI     NheI     NotI     NsiI
     NspI     PasI     PciI    PflMI     PmeI     PpiI    PshAI     PsiI
     PstI     PvuI     SacI     SalI     SbfI     ScaI     SfcI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     ApaI     AscI    AvrII     BaeI     BbeI    BbvCI
   Bpu10I    BsiWI    BsmBI  BstZ17I   Bsu36I   DraIII EcoO109I    EcoRV
     FalI     FseI    FspAI     KasI     NaeI     NarI   NgoMIV     NruI
     PacI     PfoI     PmlI    PpuMI   PspOMI    PspXI     PsrI    PvuII
    RsrII    SacII    SanDI     SapI    SexAI     SfiI     SfoI     SrfI
     SwaI     XcmI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com