Restriction map for
pFN21AB5008
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# NO CUT ENZYMES ( 135 enzymes) #
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AatII Acc65I AccI AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BalI BamHI BbeI
BbrPI BbsI BbvCI BciVI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaBI BseRI BsiEI BsiWI
BsmBI BspDI BspHI BspLU11I BsrDI
BsrFI BssSI Bst1107I BstEII BstPI
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DdeI DraI DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HaeII HindIII HpaI KasI KpnI
KspI MaeI MamI MfeI MluI
MluNI MscI MseI MspA1I MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SgrAI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI Tru9I Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccIII 347 TCCGGA T^CCGGA
AluI 317 AGCT AG^CT
AlwI 299 GGATC GGATC(4/5)
AlwNI 733 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 447 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 770 CCCGGG C^[CT]CG[GA]G
BcgI 507 CGACTGCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 668 TGATCA T^GATCA
BglI 172 GCCTTCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 182 CTCCAG CTGGAG(16/14)
BsaAI 643 TACGTG [CT]AC^GT[GA]
BsaI 225 GGTCTC GGTCTC(1/5)
BsaWI 347 TCCGGA [AT]^CCGG[AT]
BseAI 347 TCCGGA T^CCGGA
BsmAI 226 GTCTC GTCTC(1/5)
BsmI 280 GCATTC GAATGC(1/-1)
BsoBI 770 CCCGGG C^[CT]CG[GA]G
BspEI 347 TCCGGA T^CCGGA
BsrBI 142 GAGCGG CCGCTC(-3/-3)
BsrI 732 CCAGT ACTGG(1/-1)
BstAPI 854 GCACGGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 383 TTCGAA TT^CGAA
BstYI 299 GGATCT [GA]^GATC[CT]
DraIII 585 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 386 GAAGAG CTCTTC(1/4)
EcoT14I 294 CCTTGG C^C[AT][AT]GG
FbaI 668 TGATCA T^GATCA
HincII 580 GTCAAC GT[CT]^[GA]AC
HindII 580 GTCAAC GT[CT]^[GA]AC
HphI 767 TCACC GGTGA(8/7)
Ksp632I 386 GAAGAG CTCTTC(1/4)
MaeII 644 ACGT A^CGT
MboII 386 GAAGA GAAGA(8/7)
MflI 299 GGATCT [GA]^GATC[CT]
MroI 347 TCCGGA T^CCGGA
NspV 383 TTCGAA TT^CGAA
PflMI 732 CCAGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 13 GACTC GAGTC(4/5)
PstI 510 CTGCAG CTGCA^G
SacI 316 GAGCTC GAGCT^C
SfcI 510 CTGCAG C^T[GA][CT]AG
SfuI 383 TTCGAA TT^CGAA
SmaI 770 CCCGGG CCC^GGG
SmlI 58 CTCAAG C^T[CT][GA]AG
StyI 294 CCTTGG C^C[AT][AT]GG
TfiI 495 GAATC G^A[AT]TC
Tsp509I 393 AATT ^AATT
Van91I 732 CCAGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 830 CCAAGCCCAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 299 GGATCT [GA]^GATC[CT]
XmaCI 770 CCCGGG C^CCGGG
XmaI 770 CCCGGG C^CCGGG
XmnI 447 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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AcyI 208 GGCGTC G[GA]^CG[CT]C
246 GGCGTC
ApaI 104 GGGCCC GGGCC^C
820 GGGCCC
AspHI 316 GAGCTC G[AT]GC[AT]^C
793 GAGCAC
AvaII 111 GGTCC G^G[AT]CC
523 GGACC
BanI 540 GGTGCC G^G[CT][GA]CC
826 GGTGCC
BsaHI 208 GGCGTC G[GA]^CG[CT]C
246 GGCGTC
BsgI 654 CTGCAC GTGCAG(16/14)
628 GTGCAG
BsiHKAI 316 GAGCTC G[AT]GC[AT]^C
793 GAGCAC
BsmFI 11 GGGAC GGGAC(10/14)
249 GTCCC
Bsp1407I 287 TGTACA T^GTACA
761 TGTACA
BspMI 550 ACCTGC ACCTGC(4/8)
21 GCAGGT
BsrGI 287 TGTACA T^GTACA
761 TGTACA
BssHII 35 GCGCGC G^CGCGC
37 GCGCGC
Hin1I 208 GGCGTC G[GA]^CG[CT]C
246 GGCGTC
HinfI 13 GACTC G^A[ACGT]TC
495 GAATC
MaeIII 257 GTGAC ^GT[ACGT]AC
802 GTCAC
SspBI 287 TGTACA T^GTACA
761 TGTACA
TaqI 384 TCGA T^CGA
608 TCGA
Tsp45I 257 GTGAC ^GT[GC]AC
802 GTCAC
TthHB8I 384 TCGA T^CGA
608 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AccII AciI AfaI BanII BbvI
BcnI BmyI BsaJI BsiYI BslI
Bsp1286I BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DpnII DraII
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HpaII ItaI MboI MnlI MslI
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI TseI TspRI