(Circular) (Six-base) MAPSORT of: pFN21AB5008.seq Check: 1746 from: 1 to: 5587 pFN21AB5008 5587 bp With 182 enzymes: SgfI * October 20, 2009 11:00 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2017 2564 2017 Size: 547 5040 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3397 278 Size: 53 83 186 936 1861 2468 Fragments arranged by size: 2468 1861 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2930 1937 Size: 993 4594 Acc65I G'GTAC_C Cuts at: 2908 2908 Size: 5587 AclI AA'CG_TT Cuts at: 1563 3714 4087 1563 Size: 2151 373 3063 Fragments arranged by size: 3063 2151 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3650 5034 5302 1632 Size: 2018 1384 268 1917 Fragments arranged by size: 2018 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5390 AflIII A'CryG_T Cuts at: 1203 4486 5463 1203 Size: 3283 977 1327 Fragments arranged by size: 3283 1327 977 AgeI A'CCGG_T Cuts at: 4418 4418 Size: 5587 AhdI GACnn_n'nnGTC Cuts at: 4316 4316 Size: 5587 AleI CACnn'nnGTG Cuts at: 1166 2777 1166 Size: 1611 3976 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5587 AlwNI CAG_nnn'CTG Cuts at: 2743 3330 4902 2743 Size: 587 1572 3428 Fragments arranged by size: 3428 1572 587 ApaI G_GGCC'C Cuts at: 2113 2829 2113 Size: 716 4871 ApaLI G'TGCA_C Cuts at: 3644 4800 3644 Size: 1156 4431 ApoI r'AATT_y Cuts at: 1493 2896 3122 3274 5383 1493 Size: 1403 226 152 2109 1697 Fragments arranged by size: 2109 1697 1403 226 152 AseI AT'TA_AT Cuts at: 160 1032 4141 160 Size: 872 3109 1606 Fragments arranged by size: 3109 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5587 AvaI C'yCGr_G Cuts at: 1870 1943 2775 2912 1870 Size: 73 832 137 4545 Fragments arranged by size: 4545 832 137 73 BamHI G'GATC_C Cuts at: 2917 3284 2917 Size: 367 5220 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2545 2831 2908 4363 618 Size: 325 217 627 758 286 77 1455 1842 Fragments arranged by size: 1842 1455 758 627 325 286 217 77 BanII G_rGCy'C Cuts at: 1391 2113 2325 2820 2829 2906 1391 Size: 722 212 495 9 77 4072 Fragments arranged by size: 4072 722 495 212 77 9 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4616 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2501 2535 3778 3812 1903 Size: 34 50 34 480 34 1243 34 3678 Fragments arranged by size: 3678 1243 480 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3479 4689 3479 Size: 1210 4377 BclI T'GATC_A Cuts at: 1548 2673 1548 Size: 1125 4462 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2183 4198 136 Size: 107 122 71 631 605 511 2015 1525 Fragments arranged by size: 2015 1525 631 605 511 122 107 71 BglII A'GATC_T Cuts at: 5582 5582 Size: 5587 BlpI GC'TnA_GC Cuts at: 3004 3004 Size: 5587 Bme1580I G_kGCm'C Cuts at: 1790 2113 2524 2550 2829 3648 4804 1790 Size: 323 411 26 279 819 1156 2573 Fragments arranged by size: 2573 1156 819 411 323 279 26 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5587 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2731 3318 4276 Size: 416 53 83 399 24 1314 587 958 Cuts at: 4276 442 Size: 1753 Fragments arranged by size: 1753 1314 958 587 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5587 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2172 4247 1239 Size: 933 2075 2579 Fragments arranged by size: 2579 2075 933 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2048 3712 4577 4875 2048 Size: 1664 865 298 2760 Fragments arranged by size: 2760 1664 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2236 4250 882 Size: 617 737 2014 2219 Fragments arranged by size: 2219 2014 737 617 BsaAI yAC'GTr Cuts at: 493 1204 2650 3378 493 Size: 711 1446 728 2702 Fragments arranged by size: 2702 1446 728 711 BsaBI GATnn'nnATC Cuts at: 5581 5581 Size: 5587 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2214 2252 3394 3776 Size: 53 83 186 936 681 38 1142 382 Cuts at: 3776 4411 275 Size: 635 1451 Fragments arranged by size: 1451 1142 936 681 635 382 186 83 53 38 BsaWI w'CCGG_w Cuts at: 2352 4020 4418 4692 4839 5250 2352 Size: 1668 398 274 147 411 2689 Fragments arranged by size: 2689 1668 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5362 Fragments arranged by size: 5362 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5587 BseYI C'CCAG_C Cuts at: 2840 4790 5286 2840 Size: 1950 496 3141 Fragments arranged by size: 3141 1950 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2644 2654 1163 Size: 573 908 10 4096 Fragments arranged by size: 4096 908 573 10 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3037 3798 4826 1540 Size: 275 186 1036 761 1028 2301 Fragments arranged by size: 2301 1036 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2325 2802 3648 3733 4804 2325 Size: 477 846 85 1071 3108 Fragments arranged by size: 3108 1071 846 477 85 BsmI GAATG_Cn' Cuts at: 2285 3098 3191 2285 Size: 813 93 4681 Fragments arranged by size: 4681 813 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2113 2325 2524 2550 2802 2820 2829 Size: 399 323 212 199 26 252 18 9 Cuts at: 2829 2906 3648 3733 4804 1391 Size: 77 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 323 252 212 199 85 77 26 18 9 BspEI T'CCGG_A Cuts at: 2352 5250 2352 Size: 2898 2689 BspHI T'CATG_A Cuts at: 3476 5206 3476 Size: 1730 3857 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2017 2564 2942 844 Size: 1173 547 378 3489 Fragments arranged by size: 3489 1173 547 378 BsrBI CCG'CTC Cuts at: 1394 1823 2149 3474 1394 Size: 429 326 1325 3507 Fragments arranged by size: 3507 1325 429 326 BsrDI GCAATG_nn' Cuts at: 65 1248 4082 4256 65 Size: 1183 2834 174 1396 Fragments arranged by size: 2834 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4231 4418 1700 Size: 110 2421 187 2869 Fragments arranged by size: 2869 2421 187 110 BsrGI T'GTAC_A Cuts at: 96 2292 2766 96 Size: 2196 474 2917 Fragments arranged by size: 2917 2196 474 BssHII G'CGCG_C Cuts at: 1925 2040 2042 1925 Size: 115 2 5470 Fragments arranged by size: 5470 115 2 BssSI C'ACGA_G Cuts at: 3647 4659 3647 Size: 1012 4575 BstAPI GCAn_nnn'nTGC Cuts at: 794 2865 794 Size: 2071 3516 BstBI TT'CG_AA Cuts at: 2389 4391 2389 Size: 2002 3585 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5587 BstYI r'GATC_y Cuts at: 1972 2304 2917 3284 3669 3686 5138 5224 5236 Size: 332 613 367 385 17 1452 86 12 Cuts at: 5236 5582 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 613 385 367 346 332 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5035 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4848 Fragments arranged by size: 4848 683 56 BtsI GCAGTG_nn' Cuts at: 2524 3103 3897 3917 2524 Size: 579 794 20 4194 Fragments arranged by size: 4194 794 579 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5587 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2449 2484 529 Size: 35 1885 35 3632 Fragments arranged by size: 3632 1885 35 35 DraI TTT'AAA Cuts at: 2891 2891 Size: 5587 DraIII CAC_nnn'GTG Cuts at: 2595 2595 Size: 5587 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4594 809 Size: 507 3278 1802 Fragments arranged by size: 3278 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3034 3923 5552 Size: 54 1425 50 275 18 1204 889 1629 Cuts at: 5552 8 Size: 43 Fragments arranged by size: 1629 1425 1204 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3034 1537 Size: 275 1222 4090 Fragments arranged by size: 4090 1222 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2386 3517 1353 Size: 1033 1131 3423 Fragments arranged by size: 3423 1131 1033 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4174 4548 4694 387 Size: 1064 138 2585 374 146 1280 Fragments arranged by size: 2585 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2323 2323 Size: 5587 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2172 3650 4247 5034 5302 1239 Size: 393 540 1478 597 787 268 1524 Fragments arranged by size: 1524 1478 787 597 540 393 268 EcoO109I rG'GnC_Cy Cuts at: 2109 2110 2825 2109 Size: 1 715 4871 Fragments arranged by size: 4871 715 1 EcoRI G'AATT_C Cuts at: 2896 3274 2896 Size: 378 5209 FspI TGC'GCA Cuts at: 4093 4093 Size: 5587 HaeII r_GCGC'y Cuts at: 1678 4734 1678 Size: 3056 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2485 2517 4228 4260 4302 Size: 32 135 32 913 32 1711 32 42 Cuts at: 4302 4334 1373 Size: 32 2626 Fragments arranged by size: 2626 1711 913 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2587 2931 3177 669 Size: 988 281 649 344 246 3079 Fragments arranged by size: 3079 988 649 344 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5587 HpaI GTT'AAC Cuts at: 3177 3177 Size: 5587 KpnI G_GTAC'C Cuts at: 2912 2912 Size: 5587 MfeI C'AATT_G Cuts at: 3186 3186 Size: 5587 MluI A'CGCG_T Cuts at: 5463 5463 Size: 5587 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4701 4885 1305 Size: 3396 184 2007 Fragments arranged by size: 3396 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3765 Fragments arranged by size: 3765 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2225 2243 2648 2702 2777 2792 3545 Size: 648 1059 18 405 54 75 15 753 Cuts at: 3545 3904 4063 518 Size: 359 159 2042 Fragments arranged by size: 2042 1059 753 648 405 359 159 75 54 18 15 MspA1I CmG'CkG Cuts at: 1606 3003 3680 4828 5073 1606 Size: 1397 677 1148 245 2120 Fragments arranged by size: 2120 1397 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5035 NdeI CA'TA_TG Cuts at: 387 387 Size: 5587 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5587 NotI GC'GGCC_GC Cuts at: 3034 3034 Size: 5587 NspI r_CATG'y Cuts at: 1132 1229 2945 4490 1132 Size: 97 1716 1545 2229 Fragments arranged by size: 2229 1716 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5587 PciI A'CATG_T Cuts at: 4486 4486 Size: 5587 PflMI CCAn_nnn'nTGG Cuts at: 1108 2743 1108 Size: 1635 3952 PmeI GTTT'AAAC Cuts at: 2891 2891 Size: 5587 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3656 3688 5169 5201 3656 Size: 32 1481 32 4042 Fragments arranged by size: 4042 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5587 PsiI TTA'TAA Cuts at: 3157 3157 Size: 5587 PspOMI G'GGCC_C Cuts at: 2109 2825 2109 Size: 716 4871 PstI C_TGCA'G Cuts at: 830 2519 2939 830 Size: 1689 420 3478 Fragments arranged by size: 3478 1689 420 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5587 SacI G_AGCT'C Cuts at: 2325 2325 Size: 5587 SalI G'TCGA_C Cuts at: 1936 2929 1936 Size: 993 4594 SbfI CC_TGCA'GG Cuts at: 2939 2939 Size: 5587 ScaI AGT'ACT Cuts at: 3835 5547 3835 Size: 1712 3875 SfcI C'TryA_G Cuts at: 826 1045 2515 2935 4070 4751 4942 826 Size: 219 1470 420 1135 681 191 1471 Fragments arranged by size: 1471 1470 1135 681 420 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5587 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5587 SmaI CCC'GGG Cuts at: 1872 2777 2914 1872 Size: 905 137 4545 Fragments arranged by size: 4545 905 137 SmlI C'TyrA_G Cuts at: 820 1017 1943 2063 3691 4592 4854 820 Size: 197 926 120 1628 901 262 1553 Fragments arranged by size: 1628 1553 926 901 262 197 120 SnaBI TAC'GTA Cuts at: 493 3378 493 Size: 2885 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5587 SphI G_CATG'C Cuts at: 1132 2945 1132 Size: 1813 3774 SspI AAT'ATT Cuts at: 5 52 3511 5 Size: 47 3459 2081 Fragments arranged by size: 3459 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5587 StyI C'CwwG_G Cuts at: 513 1065 2299 3026 513 Size: 552 1234 727 3074 Fragments arranged by size: 3074 1234 727 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3614 3631 3784 3969 4414 325 Size: 1255 2034 17 153 185 445 1498 Fragments arranged by size: 2034 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2292 2766 3833 Size: 274 80 33 51 1443 315 474 1067 Cuts at: 3833 5545 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1067 474 315 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5547 5579 5547 Size: 32 5555 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5587 XbaI T'CTAG_A Cuts at: 2923 2923 Size: 5587 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2842 2842 Size: 5587 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5587 XmaI C'CCGG_G Cuts at: 1870 2775 2912 1870 Size: 905 137 4545 Fragments arranged by size: 4545 905 137 XmnI GAAnn'nnTTC Cuts at: 2456 3716 2456 Size: 1260 4327 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3395 276 Size: 53 83 186 936 1861 2468 Fragments arranged by size: 2468 1861 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI