Restriction map for pFN21AB5000
 (Circular) (Six-base) MAPSORT of: pFN21AB5000.seq  Check: 1533  from: 1  to: 5317



pFN21AB5000  5317 bp

 With 182 enzymes: SgfI * 

                        October 20, 2009 10:59  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3127     278 
   Size:         53      83     186     936    1591    2468

  Fragments arranged by size: 

                2468    1591     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2660    1937 
   Size:        723    4594
 
Acc65I G'GTAC_C

Cuts at:   2638    2638 
   Size:       5317
 
AclI AA'CG_TT

Cuts at:   1563    3444    3817    1563 
   Size:       1881     373    3063

  Fragments arranged by size: 

                3063    1881     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2282    3380    4764    5032    1632 
   Size:        650    1098    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1098     650     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5120
 
AflIII A'CryG_T

Cuts at:   1203    4216    5193    1203 
   Size:       3013     977    1327

  Fragments arranged by size: 

                3013    1327     977
 
AgeI A'CCGG_T

Cuts at:   4148    4148 
   Size:       5317
 
AhdI GACnn_n'nnGTC

Cuts at:   4046    4046 
   Size:       5317
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5317
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5317
 
AlwNI CAG_nnn'CTG

Cuts at:   3060    4632    3060 
   Size:       1572    3745
 
ApaLI G'TGCA_C

Cuts at:   3374    4530    3374 
   Size:       1156    4161
 
ApoI r'AATT_y

Cuts at:   1493    2353    2387    2626    2852    3004    5113    1493 
   Size:        860      34     239     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     860     239     226     152      34
 
AseI AT'TA_AT

Cuts at:    160    1032    3871     160 
   Size:        872    2839    1606

  Fragments arranged by size: 

                2839    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5317
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2642    1870 
   Size:         73     699    4545

  Fragments arranged by size: 

                4545     699      73
 
BamHI G'GATC_C

Cuts at:   2647    3014    2647 
   Size:        367    4950
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2638    4093     618 
   Size:        325     217     627     851    1455    1842

  Fragments arranged by size: 

                1842    1455     851     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2122    2341    2636    1391 
   Size:        731     219     295    4072

  Fragments arranged by size: 

                4072     731     295     219
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4346
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3508    3542    1903 
   Size:         34    1571      34    3678

  Fragments arranged by size: 

                3678    1571      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3209    4419    3209 
   Size:       1210    4107
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5317
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2157    2211    3928 
   Size:        107     122      71     631     605     485      54    1717

Cuts at:   3928     136 
   Size:       1525

  Fragments arranged by size: 

                1717    1525     631     605     485     122     107      71
                  54
 
BglII A'GATC_T

Cuts at:   5312    5312 
   Size:       5317
 
BlpI GC'TnA_GC

Cuts at:   2734    2734 
   Size:       5317
 
Bme1580I G_kGCm'C

Cuts at:   1790    3378    4534    1790 
   Size:       1588    1156    2573

  Fragments arranged by size: 

                2573    1588    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5317
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2165    2219    2371 
   Size:        416      53      83     399      24     748      54     152

Cuts at:   2371    2443    3048    4006     442 
   Size:         72     605     958    1753

  Fragments arranged by size: 

                1753     958     748     605     416     399     152      83
                  72      54      53      24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5317
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2582    3977    1239 
   Size:       1343    1395    2579

  Fragments arranged by size: 

                2579    1395    1343
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3442    4307    4605    3442 
   Size:        865     298    4154

  Fragments arranged by size: 

                4154     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    3980     882 
   Size:        617    2481    2219

  Fragments arranged by size: 

                2481    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3108     493 
   Size:        711    1904    2702

  Fragments arranged by size: 

                2702    1904     711
 
BsaBI GATnn'nnATC

Cuts at:   5311    5311 
   Size:       5317
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2326    3124    3506    4141 
   Size:         53      83     186     936     793     798     382     635

Cuts at:   4141     275 
   Size:       1451

  Fragments arranged by size: 

                1451     936     798     793     635     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   3750    4148    4422    4569    4980    3750 
   Size:        398     274     147     411    4087

  Fragments arranged by size: 

                4087     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5092

  Fragments arranged by size: 

                5092     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2327    1186 
   Size:       1141    4176
 
BseYI C'CCAG_C

Cuts at:   4520    5016    4520 
   Size:        496    4821
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    4744
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2767    3528    4556    1540 
   Size:        275     186     766     761    1028    2301

  Fragments arranged by size: 

                2301    1028     766     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3378    3463    4534    3378 
   Size:         85    1071    4161

  Fragments arranged by size: 

                4161    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2828    2921    2828 
   Size:         93    5224
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2122    2341    2636    3378    3463    4534    1391 
   Size:        399     332     219     295     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     399     332     295     219      85
            
 
BspEI T'CCGG_A

Cuts at:   4980    4980 
   Size:       5317
 
BspHI T'CATG_A

Cuts at:   3206    4936    3206 
   Size:       1730    3587
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2672     844 
   Size:       1828    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3204    1394 
   Size:        429    1381    3507

  Fragments arranged by size: 

                3507    1381     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3812    3986      65 
   Size:       1183    2564     174    1396

  Fragments arranged by size: 

                2564    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    3961    4148    1700 
   Size:        110    2151     187    2869

  Fragments arranged by size: 

                2869    2151     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5317
 
BssHII G'CGCG_C

Cuts at:   1925    2147    1925 
   Size:        222    5095
 
BssSI C'ACGA_G

Cuts at:   3377    4389    3377 
   Size:       1012    4305
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5317
 
BstBI TT'CG_AA

Cuts at:   4121    4121 
   Size:       5317
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5317
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2555    2555 
   Size:       5317
 
BstYI r'GATC_y

Cuts at:   1972    2647    3014    3399    3416    4868    4954    4966    5312 
   Size:        675     367     385      17    1452      86      12     346

Cuts at:   5312    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452     675     385     367     346      86      17
                  12
 
Bsu36I CC'TnA_GG

Cuts at:   2232    2232 
   Size:       5317
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2206    2518     513 
   Size:        552     939     202     312    3312

  Fragments arranged by size: 

                3312     939     552     312     202
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4578

  Fragments arranged by size: 

                4578     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2833    3627    3647    2833 
   Size:        794      20    4503

  Fragments arranged by size: 

                4503     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5317
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5282
 
DraI TTT'AAA

Cuts at:   2621    2621 
   Size:       5317
 
DraIII CAC_nnn'GTG

Cuts at:   2451    2451 
   Size:       5317
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4324     809 
   Size:        507    3008    1802

  Fragments arranged by size: 

                3008    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2212    2764    3653 
   Size:         54    1425      50     275      18     382     552     889

Cuts at:   3653    5282       8 
   Size:       1629      43

  Fragments arranged by size: 

                1629    1425     889     552     382     275      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2764    1537 
   Size:        275     952    4090

  Fragments arranged by size: 

                4090     952     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3247    1353 
   Size:       1894    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3904    4278    4424     387 
   Size:       1064     138    2315     374     146    1280

  Fragments arranged by size: 

                2315    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2282    2582    3380    3977    4764    5032    1239 
   Size:        393     650     300     798     597     787     268    1524

  Fragments arranged by size: 

                1524     798     787     650     597     393     300     268
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2528    2528 
   Size:       5317
 
EcoRI G'AATT_C

Cuts at:   2353    2626    3004    2353 
   Size:        273     378    4666

  Fragments arranged by size: 

                4666     378     273
 
FspI TGC'GCA

Cuts at:   3823    3823 
   Size:       5317
 
HaeII r_GCGC'y

Cuts at:   1678    4464    1678 
   Size:       2786    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    3958    3990    4032    4064    1373 
   Size:         32     135      32    2386      32      42      32    2626

  Fragments arranged by size: 

                2626    2386     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2272    2661    2907     669 
   Size:        988     281     334     389     246    3079

  Fragments arranged by size: 

                3079     988     389     334     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5317
 
HpaI GTT'AAC

Cuts at:   2272    2907    2272 
   Size:        635    4682
 
KpnI G_GTAC'C

Cuts at:   2642    2642 
   Size:       5317
 
MfeI C'AATT_G

Cuts at:   2916    2916 
   Size:       5317
 
MluI A'CGCG_T

Cuts at:   5193    5193 
   Size:       5317
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4431    4615    1305 
   Size:       3126     184    2007

  Fragments arranged by size: 

                3126    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3495

  Fragments arranged by size: 

                3495    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2265    3275    3634    3793     518 
   Size:        648    1099    1010     359     159    2042

  Fragments arranged by size: 

                2042    1099    1010     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2082    2733    3410    4558    4803    1606 
   Size:        476     651     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     677     651     476     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3826

  Fragments arranged by size: 

                3826     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5317
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5317
 
NotI GC'GGCC_GC

Cuts at:   2764    2764 
   Size:       5317
 
NspI r_CATG'y

Cuts at:   1132    1229    2675    4220    1132 
   Size:         97    1446    1545    2229

  Fragments arranged by size: 

                2229    1545    1446      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5317
 
PciI A'CATG_T

Cuts at:   4216    4216 
   Size:       5317
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5317
 
PfoI T'CCnGG_A

Cuts at:   2025    2025 
   Size:       5317
 
PmeI GTTT'AAAC

Cuts at:   2621    2621 
   Size:       5317
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3386    3418    4899    4931    3386 
   Size:         32    1481      32    3772

  Fragments arranged by size: 

                3772    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5317
 
PsiI TTA'TAA

Cuts at:   2887    2887 
   Size:       5317
 
PstI C_TGCA'G

Cuts at:    830    2506    2669     830 
   Size:       1676     163    3478

  Fragments arranged by size: 

                3478    1676     163
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5317
 
SalI G'TCGA_C

Cuts at:   1936    2659    1936 
   Size:        723    4594
 
SbfI CC_TGCA'GG

Cuts at:   2669    2669 
   Size:       5317
 
ScaI AGT'ACT

Cuts at:   3565    5277    3565 
   Size:       1712    3605
 
SfcI C'TryA_G

Cuts at:    826    1045    2502    2665    3800    4481    4672     826 
   Size:        219    1457     163    1135     681     191    1471

  Fragments arranged by size: 

                1471    1457    1135     681     219     191     163
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5317
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5317
 
SmaI CCC'GGG

Cuts at:   1872    2644    1872 
   Size:        772    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3421    4322    4584     820 
   Size:        197     926    1478     901     262    1553

  Fragments arranged by size: 

                1553    1478     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3108     493 
   Size:       2615    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5317
 
SphI G_CATG'C

Cuts at:   1132    2675    1132 
   Size:       1543    3774
 
SspI AAT'ATT

Cuts at:      5      52    3241       5 
   Size:         47    3189    2081

  Fragments arranged by size: 

                3189    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5317
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2524    2756     513 
   Size:        552     939     520     232    3074

  Fragments arranged by size: 

                3074     939     552     520     232
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    1997    2462    3344    3361    3514    3699    4144 
   Size:       1255     417     465     882      17     153     185     445

Cuts at:   4144     325 
   Size:       1498

  Fragments arranged by size: 

                1498    1255     882     465     445     417     185     153
                  17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2019    3563    5275 
   Size:        274      80      33      51    1443      42    1544    1712

Cuts at:   5275      96 
   Size:        138

  Fragments arranged by size: 

                1712    1544    1443     274     138      80      51      42
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5277    5309    5277 
   Size:         32    5285
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5317
 
XbaI T'CTAG_A

Cuts at:   2653    2653 
   Size:       5317
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2552    2555    2552 
   Size:          3    5314
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5317
 
XmaI C'CCGG_G

Cuts at:   1870    2642    1870 
   Size:        772    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3446    3446 
   Size:       5317
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3125     276 
   Size:         53      83     186     936    1591    2468

  Fragments arranged by size: 

                2468    1591     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  Eco57MI    EcoNI    EcoRI     FspI    HaeII    Hin4I   HincII
  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NcoI     NdeI     NheI     NotI     NspI     PasI     PciI
    PflMI     PfoI     PmeI     PpiI    PshAI     PsiI     PstI     PvuI
     SalI     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI    BfrBI     BplI   Bpu10I    BsiWI    BsmBI  BstZ17I  EcoICRI
 EcoO109I    EcoRV     FalI     FseI    FspAI     KasI     NaeI     NarI
   NgoMIV     NruI     NsiI     PacI     PmlI    PpuMI   PspOMI    PspXI
     PsrI    PvuII    RsrII     SacI    SacII    SanDI     SapI    SexAI
     SfiI     SfoI     SrfI     SwaI
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