Restriction map for
pFN21AB4987
(Linear) (Six-base) MAPSORT of: KIBB4987.seq Check: 9352 from: 1 to: 1413
KIBB4987.orf
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AatII G_ACGT'C
Cuts at: 0 968 1413
Size: 968 445
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 260 518 849 1413
Size: 260 258 331 564
Fragments arranged by size:
564 331 260 258
AflIII A'CryG_T
Cuts at: 0 1190 1413
Size: 1190 223
AgeI A'CCGG_T
Cuts at: 0 1279 1413
Size: 1279 134
AlwNI CAG_nnn'CTG
Cuts at: 0 238 818 1413
Size: 238 580 595
Fragments arranged by size:
595 580 238
ApaI G_GGCC'C
Cuts at: 0 490 1413
Size: 490 923
AvaI C'yCGr_G
Cuts at: 0 1219 1237 1413
Size: 1219 18 176
Fragments arranged by size:
1219 176 18
BamHI G'GATC_C
Cuts at: 0 939 1413
Size: 939 474
BanI G'GyrC_C
Cuts at: 0 1033 1413
Size: 1033 380
BanII G_rGCy'C
Cuts at: 0 17 65 490 733 1289 1413
Size: 17 48 425 243 556 124
Fragments arranged by size:
556 425 243 124 48 17
BbeI G_GCGC'C
Cuts at: 0 1037 1413
Size: 1037 376
BfrBI ATG'CAT
Cuts at: 0 507 1413
Size: 507 906
BglI GCCn_nnn'nGGC
Cuts at: 0 850 1413
Size: 850 563
BglII A'GATC_T
Cuts at: 0 884 1413
Size: 884 529
Bme1580I G_kGCm'C
Cuts at: 0 490 569 1413
Size: 490 79 844
Fragments arranged by size:
844 490 79
BmgBI CAC'GTC
Cuts at: 0 1191 1413
Size: 1191 222
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1291 1413
Size: 1291 122
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 173 532 1154 1413
Size: 49 124 359 622 259
Fragments arranged by size:
622 359 259 124 49
Bpu10I CC'TnA_GC
Cuts at: 0 732 1413
Size: 732 681
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1273 1413
Size: 1273 140
BsaBI GATnn'nnATC
Cuts at: 0 428 1413
Size: 428 985
BsaHI Gr'CG_yC
Cuts at: 0 965 1034 1413
Size: 965 69 379
Fragments arranged by size:
965 379 69
BsaWI w'CCGG_w
Cuts at: 0 1279 1413
Size: 1279 134
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1051 1081 1413
Size: 1051 30 332
Fragments arranged by size:
1051 332 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1059 1168 1413
Size: 1059 109 245
Fragments arranged by size:
1059 245 109
BseYI C'CCAG_C
Cuts at: 0 198 1020 1413
Size: 198 822 393
Fragments arranged by size:
822 393 198
BsiHKAI G_wGCw'C
Cuts at: 0 65 733 1160 1289 1334 1413
Size: 65 668 427 129 45 79
Fragments arranged by size:
668 427 129 79 65 45
BsmI GAATG_Cn'
Cuts at: 0 657 1413
Size: 657 756
Bsp1286I G_dGCh'C
Cuts at: 0 17 65 490 569 733 1160 1289 1334
Size: 17 48 425 79 164 427 129 45
Cuts at: 1334 1413
Size: 79
Fragments arranged by size:
427 425 164 129 79 79 48 45
17
BspHI T'CATG_A
Cuts at: 0 1379 1413
Size: 1379 34
BsrDI GCAATG_nn'
Cuts at: 0 509 1413
Size: 509 904
BsrFI r'CCGG_y
Cuts at: 0 370 1279 1413
Size: 370 909 134
Fragments arranged by size:
909 370 134
BsrGI T'GTAC_A
Cuts at: 0 473 1413
Size: 473 940
BstEII G'GTnAC_C
Cuts at: 0 514 1413
Size: 514 899
BstYI r'GATC_y
Cuts at: 0 884 939 1413
Size: 884 55 474
Fragments arranged by size:
884 474 55
BtgI C'CryG_G
Cuts at: 0 158 259 1413
Size: 158 101 1154
Fragments arranged by size:
1154 158 101
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 30 651 1413
Size: 30 621 762
Fragments arranged by size:
762 621 30
BtsI GCAGTG_nn'
Cuts at: 0 285 1413
Size: 285 1128
DraIII CAC_nnn'GTG
Cuts at: 0 542 1413
Size: 542 871
EaeI y'GGCC_r
Cuts at: 0 372 1091 1413
Size: 372 719 322
Fragments arranged by size:
719 372 322
EarI CTCTTCn'nnn_
Cuts at: 0 236 498 1413
Size: 236 262 915
Fragments arranged by size:
915 262 236
EcoICRI GAG'CTC
Cuts at: 0 63 731 1287 1413
Size: 63 668 556 126
Fragments arranged by size:
668 556 126 63
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 173 260 518 532 849 1154 1413
Size: 49 124 87 258 14 317 305 259
Fragments arranged by size:
317 305 259 258 124 87 49 14
EcoNI CCTnn'n_nnAGG
Cuts at: 0 625 1413
Size: 625 788
EcoO109I rG'GnC_Cy
Cuts at: 0 71 486 487 612 1413
Size: 71 415 1 125 801
Fragments arranged by size:
801 415 125 71 1
EcoRV GAT'ATC
Cuts at: 0 906 1413
Size: 906 507
FspI TGC'GCA
Cuts at: 0 397 1413
Size: 397 1016
HaeII r_GCGC'y
Cuts at: 0 1037 1413
Size: 1037 376
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 4 36 969 1001 1051 1083 1133 1165
Size: 4 32 933 32 50 32 50 32
Cuts at: 1165 1413
Size: 248
Fragments arranged by size:
933 248 50 50 32 32 32 32
4
KasI G'GCGC_C
Cuts at: 0 1033 1413
Size: 1033 380
MslI CAynn'nnrTG
Cuts at: 0 580 724 1413
Size: 580 144 689
Fragments arranged by size:
689 580 144
MspA1I CmG'CkG
Cuts at: 0 160 1265 1413
Size: 160 1105 148
Fragments arranged by size:
1105 160 148
NarI GG'CG_CC
Cuts at: 0 1034 1413
Size: 1034 379
NcoI C'CATG_G
Cuts at: 0 259 1413
Size: 259 1154
NsiI A_TGCA'T
Cuts at: 0 509 1413
Size: 509 904
NspI r_CATG'y
Cuts at: 0 511 772 1126 1413
Size: 511 261 354 287
Fragments arranged by size:
511 354 287 261
PfoI T'CCnGG_A
Cuts at: 0 548 1413
Size: 548 865
PpuMI rG'GwC_Cy
Cuts at: 0 71 612 1413
Size: 71 541 801
Fragments arranged by size:
801 541 71
PspOMI G'GGCC_C
Cuts at: 0 486 1413
Size: 486 927
PspXI vC'TCGA_Gb
Cuts at: 0 1219 1237 1413
Size: 1219 18 176
Fragments arranged by size:
1219 176 18
PstI C_TGCA'G
Cuts at: 0 117 1031 1316 1413
Size: 117 914 285 97
Fragments arranged by size:
914 285 117 97
SacI G_AGCT'C
Cuts at: 0 65 733 1289 1413
Size: 65 668 556 124
Fragments arranged by size:
668 556 124 65
SacII CC_GC'GG
Cuts at: 0 161 1413
Size: 161 1252
SbfI CC_TGCA'GG
Cuts at: 0 1031 1413
Size: 1031 382
SfcI C'TryA_G
Cuts at: 0 113 633 1027 1312 1413
Size: 113 520 394 285 101
Fragments arranged by size:
520 394 285 113 101
SfoI GGC'GCC
Cuts at: 0 1035 1413
Size: 1035 378
SgrAI Cr'CCGG_yG
Cuts at: 0 1279 1413
Size: 1279 134
SmlI C'TyrA_G
Cuts at: 0 1219 1237 1288 1413
Size: 1219 18 51 125
Fragments arranged by size:
1219 125 51 18
SphI G_CATG'C
Cuts at: 0 1126 1413
Size: 1126 287
StuI AGG'CCT
Cuts at: 0 631 1413
Size: 631 782
StyI C'CwwG_G
Cuts at: 0 259 569 656 1109 1413
Size: 259 310 87 453 304
Fragments arranged by size:
453 310 304 259 87
TatI w'GTAC_w
Cuts at: 0 267 473 1413
Size: 267 206 940
Fragments arranged by size:
940 267 206
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 374 406 872 895 904 927 1413
Size: 374 32 466 23 9 23 486
Fragments arranged by size:
486 466 374 32 23 23 9
XhoI C'TCGA_G
Cuts at: 0 1219 1237 1413
Size: 1219 18 176
Fragments arranged by size:
1219 176 18
XmnI GAAnn'nnTTC
Cuts at: 0 1375 1413
Size: 1375 38
ZraI GAC'GTC
Cuts at: 0 966 1413
Size: 966 447
Enzymes that do cut:
AatII AcuI AflIII AgeI AlwNI ApaI AvaI BamHI
BanI BanII BbeI BfrBI BglI BglII Bme1580I BmgBI
BplI BpmI Bpu10I BpuEI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiHKAI BsmI Bsp1286I BspHI BsrDI BsrFI
BsrGI BstEII BstYI BtgI BtgZI BtsI DraIII EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FspI HaeII
Hin4I KasI MslI MspA1I NarI NcoI NsiI NspI
PfoI PpuMI PspOMI PspXI PstI SacI SacII SbfI
SfcI SfoI SgrAI SmlI SphI StuI StyI TatI
TstI XhoI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AhdI AleI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BbsI BbvCI BcgI BciVI BclI BlpI BmrI
BmtI BsaI BsaAI BsgI BsiEI BsiWI BsmBI BspEI
BspMI BsrBI BssHII BssSI BstAPI BstBI BstXI BstZ17I
Bsu36I ClaI CspCI DraI DrdI EagI EciI EcoRI
FalI FseI FspAI HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI NaeI NdeI NgoMIV NheI NotI
NruI PacI PasI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PsrI PvuI PvuII RsrII SalI SanDI
SapI ScaI SexAI SfiI SgfI SmaI SnaBI SpeI
SrfI SspI SwaI TaqII Tth111I XbaI XcmI XmaI