(Circular) (Six-base) MAPSORT of: pFN21AB4223.seq Check: 1550 from: 1 to: 5953 pFN21AB4223 5953 bp With 182 enzymes: SgfI * October 20, 2009 10:59 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3048 3763 278 Size: 53 83 186 936 1512 715 2468 Fragments arranged by size: 2468 1512 936 715 186 83 53 AccI GT'mk_AC Cuts at: 1937 3296 1937 Size: 1359 4594 Acc65I G'GTAC_C Cuts at: 3274 3274 Size: 5953 AclI AA'CG_TT Cuts at: 1563 4080 4453 1563 Size: 2517 373 3063 Fragments arranged by size: 3063 2517 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2816 2846 4016 5400 5668 1632 Size: 1184 30 1170 1384 268 1917 Fragments arranged by size: 1917 1384 1184 1170 268 30 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5756 AflIII A'CryG_T Cuts at: 1203 2414 2613 4852 5829 1203 Size: 1211 199 2239 977 1327 Fragments arranged by size: 2239 1327 1211 977 199 AgeI A'CCGG_T Cuts at: 4784 4784 Size: 5953 AhdI GACnn_n'nnGTC Cuts at: 4682 4682 Size: 5953 AleI CACnn'nnGTG Cuts at: 1166 2033 1166 Size: 867 5086 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5953 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2795 2827 2795 Size: 32 5921 AlwNI CAG_nnn'CTG Cuts at: 2554 2752 3696 5268 2554 Size: 198 944 1572 3239 Fragments arranged by size: 3239 1572 944 198 ApaLI G'TGCA_C Cuts at: 4010 5166 4010 Size: 1156 4797 ApoI r'AATT_y Cuts at: 1493 2359 3026 3262 3488 3640 5749 1493 Size: 866 667 236 226 152 2109 1697 Fragments arranged by size: 2109 1697 866 667 236 226 152 AseI AT'TA_AT Cuts at: 160 1032 4507 160 Size: 872 3475 1606 Fragments arranged by size: 3475 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5953 AvaI C'yCGr_G Cuts at: 1870 1943 3278 1870 Size: 73 1335 4545 Fragments arranged by size: 4545 1335 73 BamHI G'GATC_C Cuts at: 3283 3650 3283 Size: 367 5586 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2044 3274 4729 618 Size: 325 217 627 257 1230 1455 1842 Fragments arranged by size: 1842 1455 1230 627 325 257 217 BanII G_rGCy'C Cuts at: 1391 3272 1391 Size: 1881 4072 BbeI G_GCGC'C Cuts at: 2048 2048 Size: 5953 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4982 BbvCI CC'TCA_GC Cuts at: 2132 2996 2132 Size: 864 5089 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4144 4178 1903 Size: 34 2207 34 3678 Fragments arranged by size: 3678 2207 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3845 5055 3845 Size: 1210 4743 BclI T'GATC_A Cuts at: 1548 2344 1548 Size: 796 5157 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4564 136 Size: 107 122 71 631 605 2892 1525 Fragments arranged by size: 2892 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3186 5948 3186 Size: 2762 3191 BlpI GC'TnA_GC Cuts at: 2051 2174 3370 2051 Size: 123 1196 4634 Fragments arranged by size: 4634 1196 123 Bme1580I G_kGCm'C Cuts at: 1790 4014 5170 1790 Size: 2224 1156 2573 Fragments arranged by size: 2573 2224 1156 BmgBI CAC'GTC Cuts at: 1318 2473 2866 1318 Size: 1155 393 4405 Fragments arranged by size: 4405 1155 393 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3684 4642 442 Size: 416 53 83 399 24 2267 958 1753 Fragments arranged by size: 2267 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5953 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3229 3229 Size: 5953 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2807 4613 1239 Size: 1568 1806 2579 Fragments arranged by size: 2579 1806 1568 Bpu10I CC'TnA_GC Cuts at: 2132 2996 3204 2132 Size: 864 208 4881 Fragments arranged by size: 4881 864 208 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2639 4078 4943 5241 2639 Size: 1439 865 298 3351 Fragments arranged by size: 3351 1439 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4616 882 Size: 617 3117 2219 Fragments arranged by size: 3117 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3744 493 Size: 711 2540 2702 Fragments arranged by size: 2702 2540 711 BsaBI GATnn'nnATC Cuts at: 5947 5947 Size: 5953 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2045 3045 3760 4142 Size: 53 83 186 936 512 1000 715 382 Cuts at: 4142 4777 275 Size: 635 1451 Fragments arranged by size: 1451 1000 936 715 635 512 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2974 4386 4784 5058 5205 5616 2974 Size: 1412 398 274 147 411 3311 Fragments arranged by size: 3311 1412 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5728 Fragments arranged by size: 5728 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2780 2900 2915 2933 2984 1186 Size: 1594 120 15 18 51 4155 Fragments arranged by size: 4155 1594 120 51 18 15 BseYI C'CCAG_C Cuts at: 2273 5156 5652 2273 Size: 2883 496 2574 Fragments arranged by size: 2883 2574 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2002 3129 1163 Size: 573 266 1127 3987 Fragments arranged by size: 3987 1127 573 266 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2437 3403 4164 5192 1540 Size: 275 186 436 966 761 1028 2301 Fragments arranged by size: 2301 1028 966 761 436 275 186 BsiHKAI G_wGCw'C Cuts at: 4014 4099 5170 4014 Size: 85 1071 4797 Fragments arranged by size: 4797 1071 85 BsmI GAATG_Cn' Cuts at: 3464 3557 3464 Size: 93 5860 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3272 4014 4099 5170 1391 Size: 399 1482 742 85 1071 2174 Fragments arranged by size: 2174 1482 1071 742 399 85 BspEI T'CCGG_A Cuts at: 2974 5616 2974 Size: 2642 3311 BspHI T'CATG_A Cuts at: 3842 5572 3842 Size: 1730 4223 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2277 3308 844 Size: 1433 1031 3489 Fragments arranged by size: 3489 1433 1031 BsrBI CCG'CTC Cuts at: 1394 1823 2050 2905 3840 1394 Size: 429 227 855 935 3507 Fragments arranged by size: 3507 935 855 429 227 BsrDI GCAATG_nn' Cuts at: 65 1248 2185 4448 4622 65 Size: 1183 937 2263 174 1396 Fragments arranged by size: 2263 1396 1183 937 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2436 4597 4784 1700 Size: 110 626 2161 187 2869 Fragments arranged by size: 2869 2161 626 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5953 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5953 BssSI C'ACGA_G Cuts at: 4013 5025 4013 Size: 1012 4941 BstAPI GCAn_nnn'nTGC Cuts at: 794 2752 3152 794 Size: 1958 400 3595 Fragments arranged by size: 3595 1958 400 BstBI TT'CG_AA Cuts at: 4757 4757 Size: 5953 BstEII G'GTnAC_C Cuts at: 1184 2602 1184 Size: 1418 4535 BstXI CCAn_nnnn'nTGG Cuts at: 2035 2035 Size: 5953 BstYI r'GATC_y Cuts at: 1972 3186 3283 3650 4035 4052 5504 5590 5602 Size: 1214 97 367 385 17 1452 86 12 Cuts at: 5602 5948 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1214 385 367 346 97 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2028 2304 2574 513 Size: 552 963 276 270 3892 Fragments arranged by size: 3892 963 552 276 270 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2624 487 Size: 683 56 1398 3816 Fragments arranged by size: 3816 1398 683 56 BtsI GCAGTG_nn' Cuts at: 3469 4263 4283 3469 Size: 794 20 5139 Fragments arranged by size: 5139 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5953 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5918 DraI TTT'AAA Cuts at: 3257 3257 Size: 5953 DraIII CAC_nnn'GTG Cuts at: 2866 2866 Size: 5953 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4960 809 Size: 507 3644 1802 Fragments arranged by size: 3644 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3099 3400 4289 Size: 54 1425 50 275 18 1269 301 889 Cuts at: 4289 5918 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1269 889 301 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3400 1537 Size: 275 1588 4090 Fragments arranged by size: 4090 1588 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2258 3883 1353 Size: 905 1625 3423 Fragments arranged by size: 3423 1625 905 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2492 4540 4914 5060 387 Size: 1064 138 903 2048 374 146 1280 Fragments arranged by size: 2048 1280 1064 903 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2807 2816 2846 4016 4613 5400 5668 Size: 393 1175 9 30 1170 597 787 268 Cuts at: 5668 1239 Size: 1524 Fragments arranged by size: 1524 1175 1170 787 597 393 268 30 9 EcoRI G'AATT_C Cuts at: 2359 3026 3262 3640 2359 Size: 667 236 378 4672 Fragments arranged by size: 4672 667 378 236 EcoRV GAT'ATC Cuts at: 3112 3112 Size: 5953 FspI TGC'GCA Cuts at: 4459 4459 Size: 5953 HaeII r_GCGC'y Cuts at: 1678 2048 2058 2379 2443 5100 1678 Size: 370 10 321 64 2657 2531 Fragments arranged by size: 2657 2531 370 321 64 10 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3221 3253 4594 4626 4668 Size: 32 135 32 1649 32 1341 32 42 Cuts at: 4668 4700 1373 Size: 32 2626 Fragments arranged by size: 2626 1649 1341 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3043 3297 3543 669 Size: 988 281 1105 254 246 3079 Fragments arranged by size: 3079 1105 988 281 254 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5953 HpaI GTT'AAC Cuts at: 3543 3543 Size: 5953 KasI G'GCGC_C Cuts at: 2044 2044 Size: 5953 KpnI G_GTAC'C Cuts at: 3278 3278 Size: 5953 MfeI C'AATT_G Cuts at: 3552 3552 Size: 5953 MluI A'CGCG_T Cuts at: 2414 5829 2414 Size: 3415 2538 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2037 3264 5067 5251 1305 Size: 732 1227 1803 184 2007 Fragments arranged by size: 2007 1803 1227 732 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3101 10 Size: 54 1425 343 1269 2862 Fragments arranged by size: 2862 1425 1269 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2033 2201 3911 4270 4429 518 Size: 648 867 168 1710 359 159 2042 Fragments arranged by size: 2042 1710 867 648 359 168 159 MspA1I CmG'CkG Cuts at: 1606 2824 3369 4046 5194 5439 1606 Size: 1218 545 677 1148 245 2120 Fragments arranged by size: 2120 1218 1148 677 545 245 NarI GG'CG_CC Cuts at: 2045 2045 Size: 5953 NcoI C'CATG_G Cuts at: 513 1065 2304 513 Size: 552 1239 4162 Fragments arranged by size: 4162 1239 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5953 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5953 NotI GC'GGCC_GC Cuts at: 3400 3400 Size: 5953 NspI r_CATG'y Cuts at: 1132 1229 2563 2759 3152 3311 4856 1132 Size: 97 1334 196 393 159 1545 2229 Fragments arranged by size: 2229 1545 1334 393 196 159 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5953 PciI A'CATG_T Cuts at: 4852 4852 Size: 5953 PflMI CCAn_nnn'nTGG Cuts at: 1108 2866 1108 Size: 1758 4195 PfoI T'CCnGG_A Cuts at: 2784 2784 Size: 5953 PmeI GTTT'AAAC Cuts at: 3257 3257 Size: 5953 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4022 4054 5535 5567 4022 Size: 32 1481 32 4408 Fragments arranged by size: 4408 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5953 PsiI TTA'TAA Cuts at: 3523 3523 Size: 5953 PstI C_TGCA'G Cuts at: 830 3305 830 Size: 2475 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5953 PvuII CAG'CTG Cuts at: 2824 2824 Size: 5953 SalI G'TCGA_C Cuts at: 1936 3295 1936 Size: 1359 4594 SbfI CC_TGCA'GG Cuts at: 3305 3305 Size: 5953 ScaI AGT'ACT Cuts at: 3212 4201 5913 3212 Size: 989 1712 3252 Fragments arranged by size: 3252 1712 989 SfcI C'TryA_G Cuts at: 826 1045 2224 2839 3301 4436 5117 5308 826 Size: 219 1179 615 462 1135 681 191 1471 Fragments arranged by size: 1471 1179 1135 681 615 462 219 191 SfoI GGC'GCC Cuts at: 2046 2046 Size: 5953 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5953 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5953 SmaI CCC'GGG Cuts at: 1872 3280 1872 Size: 1408 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2654 4057 4958 5220 820 Size: 197 926 711 1403 901 262 1553 Fragments arranged by size: 1553 1403 926 901 711 262 197 SnaBI TAC'GTA Cuts at: 493 3744 493 Size: 3251 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5953 SphI G_CATG'C Cuts at: 1132 3311 1132 Size: 2179 3774 SspI AAT'ATT Cuts at: 5 52 3877 5 Size: 47 3825 2081 Fragments arranged by size: 3825 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5953 StyI C'CwwG_G Cuts at: 513 1065 2304 2700 3392 513 Size: 552 1239 396 692 3074 Fragments arranged by size: 3074 1239 692 552 396 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2930 3980 3997 4150 4335 4780 325 Size: 1255 1350 1050 17 153 185 445 1498 Fragments arranged by size: 1498 1350 1255 1050 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3210 4199 5911 Size: 274 80 33 51 1443 1233 989 1712 Cuts at: 5911 96 Size: 138 Fragments arranged by size: 1712 1443 1233 989 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2906 2938 5913 5945 2906 Size: 32 2975 32 2914 Fragments arranged by size: 2975 2914 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2458 1316 Size: 1142 4811 XbaI T'CTAG_A Cuts at: 3289 3289 Size: 5953 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2405 2470 2405 Size: 65 5888 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5953 XmaI C'CCGG_G Cuts at: 1870 3278 1870 Size: 1408 4545 XmnI GAAnn'nnTTC Cuts at: 2084 2108 2615 4082 2084 Size: 24 507 1467 3955 Fragments arranged by size: 3955 1467 507 24 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3046 3761 276 Size: 53 83 186 936 1512 715 2468 Fragments arranged by size: 2468 1512 936 715 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI