Restriction map for
pFN21AB4123
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AflII AgeI AhdI Alw44I AlwNI
Aor51HI ApaLI AscI AseI AsnI
Asp700I AspEI AspI AviII AvrII
BalI BamHI BbeI BbrPI BbvCI
BclI BcnI BfaI BfrI BlnI
BsaAI BsaHI BseAI BsiEI BsiWI
BsmBI BspDI BspEI BspLU11I BsrBI
BsrDI BsrFI BssHII BssSI Bst1107I
BstEII BstPI BstUI BstXI BstZ17I
CfoI Cfr10I ClaI CpoI DraI
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
FauI FbaI FseI FspI HaeII
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI KspI MaeI
MfeI MluI MluNI MroI MscI
MunI MvnI NaeI NarI NciI
NgoMIV NheI NotI NruI NspI
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SwaI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 880 GTAGAC GT^[AC][GT]AC
AcsI 1111 AAATTT [GA]^AATT[CT]
AflIII 659 ACACGT A^C[GA][CT]GT
AocI 1315 CCTGAGG CC^T[ACGT]AGG
ApaI 1294 GGGCCC GGGCC^C
ApoI 1111 AAATTT [GA]^AATT[CT]
AspHI 732 GTGCTC G[AT]GC[AT]^C
BcgI 1005 CGATATATCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 496 GTATCC GTATCC(6/5)
BglI 23 GCCTGTCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 575 AGATCT A^GATCT
BlpI 507 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 507 GCTGAGC GC^T[ACGT]AGC
BsaBI 914 GATTAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 867 GAGACC GGTCTC(1/5)
BseRI 579 CTCCTC GAGGAG(10/8)
BsgI 1369 GTGCAG GTGCAG(16/14)
BsiHKAI 732 GTGCTC G[AT]GC[AT]^C
BsiYI 1227 CCTCAAAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 1227 CCTCAAAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 116 GTCCC GGGAC(10/14)
Bsp1407I 401 TGTACA T^GTACA
BspMI 512 GCAGGT ACCTGC(4/8)
BsrGI 401 TGTACA T^GTACA
BstAPI 1341 GCATGATATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 590 TTCGAA TT^CGAA
BstYI 575 AGATCT [GA]^GATC[CT]
Bsu36I 1315 CCTGAGG CC^T[ACGT]AGG
CelII 507 GCTGAGC GC^T[ACGT]AGC
DraII 1309 AGGCCCC [GA]G^G[ACGT]CC[CT]
DraIII 337 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 872 CCATGG C^C[GA][CT]GG
Eco81I 1315 CCTGAGG CC^T[ACGT]AGG
EcoNI 1227 CCTCAAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1309 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoT22I 888 ATGCAT ATGCA^T
HgaI 7 GCGTC GACGC(5/10)
MamI 914 GATTAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 575 AGATCT [GA]^GATC[CT]
NcoI 872 CCATGG C^CATGG
NsiI 888 ATGCAT ATGCA^T
NspV 590 TTCGAA TT^CGAA
SapI 318 GCTCTTC GCTCTTC(1/4)
SfcI 312 CTACAG C^T[GA][CT]AG
SfuI 590 TTCGAA TT^CGAA
SmlI 802 CTCAAG C^T[CT][GA]AG
SspBI 401 TGTACA T^GTACA
StuI 927 AGGCCT AGG^CCT
XhoII 575 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
Acc65I 1211 GGTACC G^GTACC
1352 GGTACC
Asp718I 1211 GGTACC G^GTACC
1352 GGTACC
AvaI 137 CTCGGG C^[CT]CG[GA]G
194 CTCGGG
AvaII 654 GGTCC G^G[AT]CC
726 GGTCC
BbsI 636 GAAGAC GAAGAC(2/6)
1390 GAAGAC
BpmI 18 CTCCAG CTGGAG(16/14)
1046 CTGGAG
BpuAI 636 GAAGAC GAAGAC(2/6)
1390 GAAGAC
BsaWI 728 TCCGGT [AT]^CCGG[AT]
1208 TCCGGT
BsmI 1266 GAATGC GAATGC(1/-1)
890 GCATTC
BsoBI 137 CTCGGG C^[CT]CG[GA]G
194 CTCGGG
BspHI 369 TCATGA T^CATGA
1262 TCATGA
BsrI 677 ACTGG ACTGG(1/-1)
1045 ACTGG
BssKI 265 CCAGG ^CC[ACGT]GG
585 CCTGG
BstNI 265 CCAGG CC^[AT]GG
585 CCTGG
EcoRII 265 CCAGG ^CC[AT]GG
585 CCTGG
EcoT14I 720 CCAAGG C^C[AT][AT]GG
872 CCATGG
KpnI 1211 GGTACC GGTAC^C
1352 GGTACC
MspA1I 256 CAGCGG C[AC]G^C[GT]G
563 CAGCGG
MvaI 265 CCAGG CC^[AT]GG
585 CCTGG
NdeI 885 CATATG CA^TATG
1094 CATATG
PleI 361 GAGTC GAGTC(4/5)
938 GAGTC
RcaI 369 TCATGA T^CATGA
1262 TCATGA
ScrFI 265 CCAGG CC^[ACGT]GG
585 CCTGG
SspI 161 AATATT AAT^ATT
686 AATATT
StyI 720 CCAAGG C^C[AT][AT]GG
872 CCATGG
TaqI 364 TCGA T^CGA
591 TCGA
TfiI 125 GAATC G^A[AT]TC
366 GATTC
TthHB8I 364 TCGA T^CGA
591 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AfaI AluI AlwI BanI
BanII BbvI BmyI BsaJI BsmAI
Bsp1286I BstF5I Cac8I Cfr13I DdeI
DpnII EarI Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MnlI MseI MslI MspI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I SfaNI Tru9I TseI Tsp45I
Tsp509I TspRI