(Circular) (Six-base) MAPSORT of: pFN21AB4123.seq Check: 8604 from: 1 to: 6109 pFN21AB4123 6109 bp With 182 enzymes: SgfI * October 20, 2009 10:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3919 278 Size: 53 83 186 936 2383 2468 Fragments arranged by size: 2468 2383 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2886 3452 1937 Size: 949 566 4594 Fragments arranged by size: 4594 949 566 Acc65I G'GTAC_C Cuts at: 3216 3357 3430 3216 Size: 141 73 5895 Fragments arranged by size: 5895 141 73 AclI AA'CG_TT Cuts at: 1563 4236 4609 1563 Size: 2673 373 3063 Fragments arranged by size: 3063 2673 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2079 4172 5556 5824 1632 Size: 447 2093 1384 268 1917 Fragments arranged by size: 2093 1917 1384 447 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5912 AflIII A'CryG_T Cuts at: 1203 2664 5008 5985 1203 Size: 1461 2344 977 1327 Fragments arranged by size: 2344 1461 1327 977 AgeI A'CCGG_T Cuts at: 4940 4940 Size: 6109 AhdI GACnn_n'nnGTC Cuts at: 4838 4838 Size: 6109 AleI CACnn'nnGTG Cuts at: 1166 2570 2683 2685 1166 Size: 1404 113 2 4590 Fragments arranged by size: 4590 1404 113 2 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6109 AlwNI CAG_nnn'CTG Cuts at: 3852 5424 3852 Size: 1572 4537 ApaI G_GGCC'C Cuts at: 3303 3303 Size: 6109 ApaLI G'TGCA_C Cuts at: 4166 5322 4166 Size: 1156 4953 ApoI r'AATT_y Cuts at: 1493 3116 3418 3644 3796 5905 1493 Size: 1623 302 226 152 2109 1697 Fragments arranged by size: 2109 1697 1623 302 226 152 AseI AT'TA_AT Cuts at: 160 1032 4663 160 Size: 872 3631 1606 Fragments arranged by size: 3631 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6109 AvaI C'yCGr_G Cuts at: 1870 1943 2142 2199 3434 1870 Size: 73 199 57 1235 4545 Fragments arranged by size: 4545 1235 199 73 57 BamHI G'GATC_C Cuts at: 3439 3806 3439 Size: 367 5742 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2113 2237 3216 3357 3430 Size: 325 217 627 326 124 979 141 73 Cuts at: 3430 4885 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 979 627 326 325 217 141 124 73 BanII G_rGCy'C Cuts at: 1391 2718 3058 3303 3428 1391 Size: 1327 340 245 125 4072 Fragments arranged by size: 4072 1327 340 245 125 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2648 3402 928 Size: 971 749 754 3635 Fragments arranged by size: 3635 971 754 749 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2999 3033 4300 4334 1903 Size: 34 1062 34 1267 34 3678 Fragments arranged by size: 3678 1267 1062 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2512 4001 5211 2512 Size: 1489 1210 3410 Fragments arranged by size: 3410 1489 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6109 BfrBI ATG'CAT Cuts at: 2895 2895 Size: 6109 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2034 4720 136 Size: 107 122 71 631 605 362 2686 1525 Fragments arranged by size: 2686 1525 631 605 362 122 107 71 BglII A'GATC_T Cuts at: 2580 6104 2580 Size: 3524 2585 BlpI GC'TnA_GC Cuts at: 2513 3526 2513 Size: 1013 5096 Bme1580I G_kGCm'C Cuts at: 1790 3303 4170 5326 1790 Size: 1513 867 1156 2573 Fragments arranged by size: 2573 1513 1156 867 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6109 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3840 4798 442 Size: 416 53 83 399 24 2423 958 1753 Fragments arranged by size: 2423 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6109 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2008 3072 4769 1239 Size: 769 1064 1697 2579 Fragments arranged by size: 2579 1697 1064 769 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2792 4234 5099 5397 2792 Size: 1442 865 298 3504 Fragments arranged by size: 3504 1442 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2866 4772 882 Size: 617 1367 1906 2219 Fragments arranged by size: 2219 1906 1367 617 BsaAI yAC'GTr Cuts at: 493 1204 3900 493 Size: 711 2696 2702 Fragments arranged by size: 2702 2696 711 BsaBI GATnn'nnATC Cuts at: 2923 6103 2923 Size: 3180 2929 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3916 4298 4933 275 Size: 53 83 186 936 2383 382 635 1451 Fragments arranged by size: 2383 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2733 3213 4542 4940 5214 5361 5772 2733 Size: 480 1329 398 274 147 411 3070 Fragments arranged by size: 3070 1329 480 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5884 Fragments arranged by size: 5884 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2575 1186 Size: 1389 4720 BseYI C'CCAG_C Cuts at: 5312 5808 5312 Size: 496 5613 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3395 1163 Size: 573 1659 3877 Fragments arranged by size: 3877 1659 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3559 4320 5348 1540 Size: 275 186 1558 761 1028 2301 Fragments arranged by size: 2301 1558 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2741 4170 4255 5326 2741 Size: 1429 85 1071 3524 Fragments arranged by size: 3524 1429 1071 85 BsmI GAATG_Cn' Cuts at: 2895 3277 3620 3713 2895 Size: 382 343 93 5291 Fragments arranged by size: 5291 382 343 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2718 2741 3058 3303 3428 4170 4255 Size: 399 928 23 317 245 125 742 85 Cuts at: 4255 5326 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 928 742 399 317 245 125 85 23 BspEI T'CCGG_A Cuts at: 5772 5772 Size: 6109 BspHI T'CATG_A Cuts at: 2374 3267 3998 5728 2374 Size: 893 731 1730 2755 Fragments arranged by size: 2755 1730 893 731 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2508 3464 844 Size: 1664 956 3489 Fragments arranged by size: 3489 1664 956 BsrBI CCG'CTC Cuts at: 1394 1823 3996 1394 Size: 429 2173 3507 Fragments arranged by size: 3507 2173 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4604 4778 65 Size: 1183 3356 174 1396 Fragments arranged by size: 3356 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4753 4940 1700 Size: 110 2943 187 2869 Fragments arranged by size: 2943 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2406 96 Size: 2310 3799 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6109 BssSI C'ACGA_G Cuts at: 4169 5181 4169 Size: 1012 5097 BstAPI GCAn_nnn'nTGC Cuts at: 794 3352 794 Size: 2558 3551 BstBI TT'CG_AA Cuts at: 2596 4913 2596 Size: 2317 3792 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6109 BstYI r'GATC_y Cuts at: 1972 2580 3439 3806 4191 4208 5660 5746 5758 Size: 608 859 367 385 17 1452 86 12 Cuts at: 5758 6104 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 859 608 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3321 3321 Size: 6109 BtgI C'CryG_G Cuts at: 513 1065 2004 2877 513 Size: 552 939 873 3745 Fragments arranged by size: 3745 939 873 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2704 487 Size: 683 56 1478 3892 Fragments arranged by size: 3892 1478 683 56 BtsI GCAGTG_nn' Cuts at: 3625 4419 4439 3625 Size: 794 20 5295 Fragments arranged by size: 5295 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6109 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6074 DraI TTT'AAA Cuts at: 3413 3413 Size: 6109 DraIII CAC_nnn'GTG Cuts at: 2347 2347 Size: 6109 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5116 809 Size: 507 3800 1802 Fragments arranged by size: 3800 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3556 4445 6074 Size: 54 1425 50 275 18 1726 889 1629 Cuts at: 6074 8 Size: 43 Fragments arranged by size: 1726 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3556 1537 Size: 275 1744 4090 Fragments arranged by size: 4090 1744 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2170 2330 2351 4039 1353 Size: 817 160 21 1688 3423 Fragments arranged by size: 3423 1688 817 160 21 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2225 4696 5070 5216 387 Size: 1064 138 636 2471 374 146 1280 Fragments arranged by size: 2471 1280 1064 636 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2008 2079 3072 4172 4769 5556 5824 Size: 393 376 71 993 1100 597 787 268 Cuts at: 5824 1239 Size: 1524 Fragments arranged by size: 1524 1100 993 787 597 393 376 268 71 EcoNI CCTnn'n_nnAGG Cuts at: 3236 3236 Size: 6109 EcoO109I rG'GnC_Cy Cuts at: 3315 3315 Size: 6109 EcoRI G'AATT_C Cuts at: 3418 3796 3418 Size: 378 5731 FspI TGC'GCA Cuts at: 4615 4615 Size: 6109 HaeII r_GCGC'y Cuts at: 1678 5256 1678 Size: 3578 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2182 2214 2362 2394 4750 Size: 32 135 32 610 32 148 32 2356 Cuts at: 4750 4782 4824 4856 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2356 610 148 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3453 3699 669 Size: 988 281 1515 246 3079 Fragments arranged by size: 3079 1515 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6109 HpaI GTT'AAC Cuts at: 3699 3699 Size: 6109 KpnI G_GTAC'C Cuts at: 3220 3361 3434 3220 Size: 141 73 5895 Fragments arranged by size: 5895 141 73 MfeI C'AATT_G Cuts at: 3708 3708 Size: 6109 MluI A'CGCG_T Cuts at: 5985 5985 Size: 6109 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5223 5407 1305 Size: 3918 184 2007 Fragments arranged by size: 3918 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4287 Fragments arranged by size: 4287 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2570 2683 2685 3308 4067 4426 4585 Size: 648 1404 113 2 623 759 359 159 Cuts at: 4585 518 Size: 2042 Fragments arranged by size: 2042 1404 759 648 623 359 159 113 2 MspA1I CmG'CkG Cuts at: 1606 2263 2570 3525 4202 5350 5595 1606 Size: 657 307 955 677 1148 245 2120 Fragments arranged by size: 2120 1148 955 677 657 307 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2877 513 Size: 552 939 873 3745 Fragments arranged by size: 3745 939 873 552 NdeI CA'TA_TG Cuts at: 387 2891 3100 387 Size: 2504 209 3396 Fragments arranged by size: 3396 2504 209 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6109 NotI GC'GGCC_GC Cuts at: 3556 3556 Size: 6109 NsiI A_TGCA'T Cuts at: 2897 2897 Size: 6109 NspI r_CATG'y Cuts at: 1132 1229 3467 5012 1132 Size: 97 2238 1545 2229 Fragments arranged by size: 2238 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6109 PciI A'CATG_T Cuts at: 5008 5008 Size: 6109 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6109 PmeI GTTT'AAAC Cuts at: 3413 3413 Size: 6109 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4178 4210 5691 5723 4178 Size: 32 1481 32 4564 Fragments arranged by size: 4564 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6109 PsiI TTA'TAA Cuts at: 3679 3679 Size: 6109 PspOMI G'GGCC_C Cuts at: 3299 3299 Size: 6109 PstI C_TGCA'G Cuts at: 830 3461 830 Size: 2631 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6109 SalI G'TCGA_C Cuts at: 1936 3451 1936 Size: 1515 4594 SapI GCTCTTCn'nnn_ Cuts at: 2330 2330 Size: 6109 SbfI CC_TGCA'GG Cuts at: 3461 3461 Size: 6109 ScaI AGT'ACT Cuts at: 4357 6069 4357 Size: 1712 4397 SfcI C'TryA_G Cuts at: 826 1045 2317 3457 4592 5273 5464 826 Size: 219 1272 1140 1135 681 191 1471 Fragments arranged by size: 1471 1272 1140 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6109 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6109 SmaI CCC'GGG Cuts at: 1872 3436 1872 Size: 1564 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2807 4213 5114 5376 820 Size: 197 926 864 1406 901 262 1553 Fragments arranged by size: 1553 1406 926 901 864 262 197 SnaBI TAC'GTA Cuts at: 493 3900 493 Size: 3407 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6109 SphI G_CATG'C Cuts at: 1132 3467 1132 Size: 2335 3774 SspI AAT'ATT Cuts at: 5 52 2168 2693 4033 5 Size: 47 2116 525 1340 2081 Fragments arranged by size: 2116 2081 1340 525 47 StuI AGG'CCT Cuts at: 1517 2934 1517 Size: 1417 4692 StyI C'CwwG_G Cuts at: 513 1065 2004 2725 2877 3548 513 Size: 552 939 721 152 671 3074 Fragments arranged by size: 3074 939 721 671 552 152 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2339 4136 4153 4306 4491 4936 325 Size: 1255 759 1797 17 153 185 445 1498 Fragments arranged by size: 1797 1498 1255 759 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2406 2430 2826 Size: 274 80 33 51 1443 429 24 396 Cuts at: 2826 4355 6067 96 Size: 1529 1712 138 Fragments arranged by size: 1712 1529 1443 429 396 274 138 80 51 33 24 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6069 6101 6069 Size: 32 6077 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6109 XbaI T'CTAG_A Cuts at: 3445 3445 Size: 6109 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6109 XmaI C'CCGG_G Cuts at: 1870 3434 1870 Size: 1564 4545 XmnI GAAnn'nnTTC Cuts at: 4238 4238 Size: 6109 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3917 276 Size: 53 83 186 936 2383 2468 Fragments arranged by size: 2468 2383 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI