Restriction map for pFN21AB4123
 (Circular) (Six-base) MAPSORT of: pFN21AB4123.seq  Check: 8604  from: 1  to: 6109



pFN21AB4123  6109 bp

 With 182 enzymes: SgfI * 

                        October 20, 2009 10:54  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3919     278 
   Size:         53      83     186     936    2383    2468

  Fragments arranged by size: 

                2468    2383     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2886    3452    1937 
   Size:        949     566    4594

  Fragments arranged by size: 

                4594     949     566
 
Acc65I G'GTAC_C

Cuts at:   3216    3357    3430    3216 
   Size:        141      73    5895

  Fragments arranged by size: 

                5895     141      73
 
AclI AA'CG_TT

Cuts at:   1563    4236    4609    1563 
   Size:       2673     373    3063

  Fragments arranged by size: 

                3063    2673     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2079    4172    5556    5824    1632 
   Size:        447    2093    1384     268    1917

  Fragments arranged by size: 

                2093    1917    1384     447     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5912
 
AflIII A'CryG_T

Cuts at:   1203    2664    5008    5985    1203 
   Size:       1461    2344     977    1327

  Fragments arranged by size: 

                2344    1461    1327     977
 
AgeI A'CCGG_T

Cuts at:   4940    4940 
   Size:       6109
 
AhdI GACnn_n'nnGTC

Cuts at:   4838    4838 
   Size:       6109
 
AleI CACnn'nnGTG

Cuts at:   1166    2570    2683    2685    1166 
   Size:       1404     113       2    4590

  Fragments arranged by size: 

                4590    1404     113       2
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       6109
 
AlwNI CAG_nnn'CTG

Cuts at:   3852    5424    3852 
   Size:       1572    4537
 
ApaI G_GGCC'C

Cuts at:   3303    3303 
   Size:       6109
 
ApaLI G'TGCA_C

Cuts at:   4166    5322    4166 
   Size:       1156    4953
 
ApoI r'AATT_y

Cuts at:   1493    3116    3418    3644    3796    5905    1493 
   Size:       1623     302     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1623     302     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4663     160 
   Size:        872    3631    1606

  Fragments arranged by size: 

                3631    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6109
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2142    2199    3434    1870 
   Size:         73     199      57    1235    4545

  Fragments arranged by size: 

                4545    1235     199      73      57
 
BamHI G'GATC_C

Cuts at:   3439    3806    3439 
   Size:        367    5742
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2113    2237    3216    3357    3430 
   Size:        325     217     627     326     124     979     141      73

Cuts at:   3430    4885     618 
   Size:       1455    1842

  Fragments arranged by size: 

                1842    1455     979     627     326     325     217     141
                 124      73
 
BanII G_rGCy'C

Cuts at:   1391    2718    3058    3303    3428    1391 
   Size:       1327     340     245     125    4072

  Fragments arranged by size: 

                4072    1327     340     245     125
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2648    3402     928 
   Size:        971     749     754    3635

  Fragments arranged by size: 

                3635     971     754     749
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    2999    3033    4300    4334    1903 
   Size:         34    1062      34    1267      34    3678

  Fragments arranged by size: 

                3678    1267    1062      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   2512    4001    5211    2512 
   Size:       1489    1210    3410

  Fragments arranged by size: 

                3410    1489    1210
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       6109
 
BfrBI ATG'CAT

Cuts at:   2895    2895 
   Size:       6109
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2034    4720     136 
   Size:        107     122      71     631     605     362    2686    1525

  Fragments arranged by size: 

                2686    1525     631     605     362     122     107      71
            
 
BglII A'GATC_T

Cuts at:   2580    6104    2580 
   Size:       3524    2585
 
BlpI GC'TnA_GC

Cuts at:   2513    3526    2513 
   Size:       1013    5096
 
Bme1580I G_kGCm'C

Cuts at:   1790    3303    4170    5326    1790 
   Size:       1513     867    1156    2573

  Fragments arranged by size: 

                2573    1513    1156     867
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       6109
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3840    4798     442 
   Size:        416      53      83     399      24    2423     958    1753

  Fragments arranged by size: 

                2423    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       6109
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2008    3072    4769    1239 
   Size:        769    1064    1697    2579

  Fragments arranged by size: 

                2579    1697    1064     769
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2792    4234    5099    5397    2792 
   Size:       1442     865     298    3504

  Fragments arranged by size: 

                3504    1442     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2866    4772     882 
   Size:        617    1367    1906    2219

  Fragments arranged by size: 

                2219    1906    1367     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3900     493 
   Size:        711    2696    2702

  Fragments arranged by size: 

                2702    2696     711
 
BsaBI GATnn'nnATC

Cuts at:   2923    6103    2923 
   Size:       3180    2929
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3916    4298    4933     275 
   Size:         53      83     186     936    2383     382     635    1451

  Fragments arranged by size: 

                2383    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   2733    3213    4542    4940    5214    5361    5772    2733 
   Size:        480    1329     398     274     147     411    3070

  Fragments arranged by size: 

                3070    1329     480     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5884

  Fragments arranged by size: 

                5884     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2575    1186 
   Size:       1389    4720
 
BseYI C'CCAG_C

Cuts at:   5312    5808    5312 
   Size:        496    5613
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    3395    1163 
   Size:        573    1659    3877

  Fragments arranged by size: 

                3877    1659     573
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3559    4320    5348    1540 
   Size:        275     186    1558     761    1028    2301

  Fragments arranged by size: 

                2301    1558    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2741    4170    4255    5326    2741 
   Size:       1429      85    1071    3524

  Fragments arranged by size: 

                3524    1429    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2895    3277    3620    3713    2895 
   Size:        382     343      93    5291

  Fragments arranged by size: 

                5291     382     343      93
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2718    2741    3058    3303    3428    4170    4255 
   Size:        399     928      23     317     245     125     742      85

Cuts at:   4255    5326    1391 
   Size:       1071    2174

  Fragments arranged by size: 

                2174    1071     928     742     399     317     245     125
                  85      23
 
BspEI T'CCGG_A

Cuts at:   5772    5772 
   Size:       6109
 
BspHI T'CATG_A

Cuts at:   2374    3267    3998    5728    2374 
   Size:        893     731    1730    2755

  Fragments arranged by size: 

                2755    1730     893     731
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2508    3464     844 
   Size:       1664     956    3489

  Fragments arranged by size: 

                3489    1664     956
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3996    1394 
   Size:        429    2173    3507

  Fragments arranged by size: 

                3507    2173     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4604    4778      65 
   Size:       1183    3356     174    1396

  Fragments arranged by size: 

                3356    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4753    4940    1700 
   Size:        110    2943     187    2869

  Fragments arranged by size: 

                2943    2869     187     110
 
BsrGI T'GTAC_A

Cuts at:     96    2406      96 
   Size:       2310    3799
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       6109
 
BssSI C'ACGA_G

Cuts at:   4169    5181    4169 
   Size:       1012    5097
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    3352     794 
   Size:       2558    3551
 
BstBI TT'CG_AA

Cuts at:   2596    4913    2596 
   Size:       2317    3792
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       6109
 
BstYI r'GATC_y

Cuts at:   1972    2580    3439    3806    4191    4208    5660    5746    5758 
   Size:        608     859     367     385      17    1452      86      12

Cuts at:   5758    6104    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     859     608     385     367     346      86
                  17      12
 
Bsu36I CC'TnA_GG

Cuts at:   3321    3321 
   Size:       6109
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2877     513 
   Size:        552     939     873    3745

  Fragments arranged by size: 

                3745     939     873     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    2704     487 
   Size:        683      56    1478    3892

  Fragments arranged by size: 

                3892    1478     683      56
 
BtsI GCAGTG_nn'

Cuts at:   3625    4419    4439    3625 
   Size:        794      20    5295

  Fragments arranged by size: 

                5295     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       6109
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    6074
 
DraI TTT'AAA

Cuts at:   3413    3413 
   Size:       6109
 
DraIII CAC_nnn'GTG

Cuts at:   2347    2347 
   Size:       6109
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    5116     809 
   Size:        507    3800    1802

  Fragments arranged by size: 

                3800    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    3556    4445    6074 
   Size:         54    1425      50     275      18    1726     889    1629

Cuts at:   6074       8 
   Size:         43

  Fragments arranged by size: 

                1726    1629    1425     889     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3556    1537 
   Size:        275    1744    4090

  Fragments arranged by size: 

                4090    1744     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2170    2330    2351    4039    1353 
   Size:        817     160      21    1688    3423

  Fragments arranged by size: 

                3423    1688     817     160      21
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    2225    4696    5070    5216     387 
   Size:       1064     138     636    2471     374     146    1280

  Fragments arranged by size: 

                2471    1280    1064     636     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2008    2079    3072    4172    4769    5556    5824 
   Size:        393     376      71     993    1100     597     787     268

Cuts at:   5824    1239 
   Size:       1524

  Fragments arranged by size: 

                1524    1100     993     787     597     393     376     268
                  71
 
EcoNI CCTnn'n_nnAGG

Cuts at:   3236    3236 
   Size:       6109
 
EcoO109I rG'GnC_Cy

Cuts at:   3315    3315 
   Size:       6109
 
EcoRI G'AATT_C

Cuts at:   3418    3796    3418 
   Size:        378    5731
 
FspI TGC'GCA

Cuts at:   4615    4615 
   Size:       6109
 
HaeII r_GCGC'y

Cuts at:   1678    5256    1678 
   Size:       3578    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2182    2214    2362    2394    4750 
   Size:         32     135      32     610      32     148      32    2356

Cuts at:   4750    4782    4824    4856    1373 
   Size:         32      42      32    2626

  Fragments arranged by size: 

                2626    2356     610     148     135      42      32      32
                  32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3453    3699     669 
   Size:        988     281    1515     246    3079

  Fragments arranged by size: 

                3079    1515     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       6109
 
HpaI GTT'AAC

Cuts at:   3699    3699 
   Size:       6109
 
KpnI G_GTAC'C

Cuts at:   3220    3361    3434    3220 
   Size:        141      73    5895

  Fragments arranged by size: 

                5895     141      73
 
MfeI C'AATT_G

Cuts at:   3708    3708 
   Size:       6109
 
MluI A'CGCG_T

Cuts at:   5985    5985 
   Size:       6109
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    5223    5407    1305 
   Size:       3918     184    2007

  Fragments arranged by size: 

                3918    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    4287

  Fragments arranged by size: 

                4287    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2570    2683    2685    3308    4067    4426    4585 
   Size:        648    1404     113       2     623     759     359     159

Cuts at:   4585     518 
   Size:       2042

  Fragments arranged by size: 

                2042    1404     759     648     623     359     159     113
                   2
 
MspA1I CmG'CkG

Cuts at:   1606    2263    2570    3525    4202    5350    5595    1606 
   Size:        657     307     955     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     955     677     657     307     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2004    2877     513 
   Size:        552     939     873    3745

  Fragments arranged by size: 

                3745     939     873     552
 
NdeI CA'TA_TG

Cuts at:    387    2891    3100     387 
   Size:       2504     209    3396

  Fragments arranged by size: 

                3396    2504     209
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       6109
 
NotI GC'GGCC_GC

Cuts at:   3556    3556 
   Size:       6109
 
NsiI A_TGCA'T

Cuts at:   2897    2897 
   Size:       6109
 
NspI r_CATG'y

Cuts at:   1132    1229    3467    5012    1132 
   Size:         97    2238    1545    2229

  Fragments arranged by size: 

                2238    2229    1545      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       6109
 
PciI A'CATG_T

Cuts at:   5008    5008 
   Size:       6109
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       6109
 
PmeI GTTT'AAAC

Cuts at:   3413    3413 
   Size:       6109
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4178    4210    5691    5723    4178 
   Size:         32    1481      32    4564

  Fragments arranged by size: 

                4564    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       6109
 
PsiI TTA'TAA

Cuts at:   3679    3679 
   Size:       6109
 
PspOMI G'GGCC_C

Cuts at:   3299    3299 
   Size:       6109
 
PstI C_TGCA'G

Cuts at:    830    3461     830 
   Size:       2631    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       6109
 
SalI G'TCGA_C

Cuts at:   1936    3451    1936 
   Size:       1515    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   2330    2330 
   Size:       6109
 
SbfI CC_TGCA'GG

Cuts at:   3461    3461 
   Size:       6109
 
ScaI AGT'ACT

Cuts at:   4357    6069    4357 
   Size:       1712    4397
 
SfcI C'TryA_G

Cuts at:    826    1045    2317    3457    4592    5273    5464     826 
   Size:        219    1272    1140    1135     681     191    1471

  Fragments arranged by size: 

                1471    1272    1140    1135     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6109
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       6109
 
SmaI CCC'GGG

Cuts at:   1872    3436    1872 
   Size:       1564    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2807    4213    5114    5376     820 
   Size:        197     926     864    1406     901     262    1553

  Fragments arranged by size: 

                1553    1406     926     901     864     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3900     493 
   Size:       3407    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       6109
 
SphI G_CATG'C

Cuts at:   1132    3467    1132 
   Size:       2335    3774
 
SspI AAT'ATT

Cuts at:      5      52    2168    2693    4033       5 
   Size:         47    2116     525    1340    2081

  Fragments arranged by size: 

                2116    2081    1340     525      47
 
StuI AGG'CCT

Cuts at:   1517    2934    1517 
   Size:       1417    4692
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2725    2877    3548     513 
   Size:        552     939     721     152     671    3074

  Fragments arranged by size: 

                3074     939     721     671     552     152
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2339    4136    4153    4306    4491    4936     325 
   Size:       1255     759    1797      17     153     185     445    1498

  Fragments arranged by size: 

                1797    1498    1255     759     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2406    2430    2826 
   Size:        274      80      33      51    1443     429      24     396

Cuts at:   2826    4355    6067      96 
   Size:       1529    1712     138

  Fragments arranged by size: 

                1712    1529    1443     429     396     274     138      80
                  51      33      24
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   6069    6101    6069 
   Size:         32    6077
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       6109
 
XbaI T'CTAG_A

Cuts at:   3445    3445 
   Size:       6109
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       6109
 
XmaI C'CCGG_G

Cuts at:   1870    3434    1870 
   Size:       1564    4545
 
XmnI GAAnn'nnTTC

Cuts at:   4238    4238 
   Size:       6109
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3917     276 
   Size:         53      83     186     936    2383    2468

  Fragments arranged by size: 

                2468    2383     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstYI   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  Eco57MI    EcoNI EcoO109I    EcoRI     FspI    HaeII
    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NcoI     NdeI     NheI     NotI     NsiI
     NspI     PasI     PciI    PflMI     PmeI     PpiI    PshAI     PsiI
   PspOMI     PstI     PvuI     SalI     SapI     SbfI     ScaI     SfcI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI
     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     AscI    AvrII     BaeI     BbeI    BbvCI
     BplI   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I  EcoICRI    EcoRV
     FalI     FseI    FspAI     KasI     NaeI     NarI   NgoMIV     NruI
     PacI     PfoI     PmlI    PpuMI    PspXI     PsrI    PvuII    RsrII
     SacI    SacII    SanDI    SexAI     SfiI     SfoI     SrfI     SwaI
     XcmI
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