Restriction map for
pFN21AB4044
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# NO CUT ENZYMES ( 136 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
AocI Aor51HI ApaI AscI Asp718I
AspEI AspI AvaI AviII BanI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BfrI BglI BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiWI BsmAI BsmBI
BsoBI Bsp1407I BspDI BspEI BspHI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CfoI Cfr10I ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI PvuII RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tth111I XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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Alw44I 1440 GTGCAC G^TGCAC
ApaLI 1440 GTGCAC G^TGCAC
AseI 91 ATTAAT AT^TAAT
AsnI 91 ATTAAT AT^TAAT
Asp700I 1165 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 1278 GGACC G^G[AT]CC
AvrII 1465 CCTAGG C^CTAGG
BalI 483 TGGCCA TGG^CCA
BamHI 540 GGATCC G^GATCC
BcnI 1067 CCGGG CC^[GC]GG
BglII 815 AGATCT A^GATCT
BlnI 1465 CCTAGG C^CTAGG
BlpI 971 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 971 GCTTAGC GC^T[ACGT]AGC
BseRI 740 GAGGAG GAGGAG(10/8)
BsgI 586 CTGCAC GTGCAG(16/14)
BsmI 1432 GAATGC GAATGC(1/-1)
BsrBI 1084 GAGCGG CCGCTC(-3/-3)
BstAPI 1479 GCAGCATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 605 TTCGAA TT^CGAA
CelII 971 GCTTAGC GC^T[ACGT]AGC
DsaI 794 CCATGG C^C[GA][CT]GG
HapII 1067 CCGG C^CGG
HincII 1015 GTTGAC GT[CT]^[GA]AC
HindII 1015 GTTGAC GT[CT]^[GA]AC
HpaII 1067 CCGG C^CGG
MluNI 483 TGGCCA TGG^CCA
MscI 483 TGGCCA TGG^CCA
MspI 1067 CCGG C^CGG
MwoI 1479 GCAGCATGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 1067 CCGGG CC^[GC]GG
NcoI 794 CCATGG C^CATGG
NspV 605 TTCGAA TT^CGAA
PflMI 528 CCATGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 91 ATTAAT AT^TAAT
SacI 743 GAGCTC GAGCT^C
SfuI 605 TTCGAA TT^CGAA
SmlI 1138 CTCAAG C^T[CT][GA]AG
StuI 164 AGGCCT AGG^CCT
TaqI 606 TCGA T^CGA
TthHB8I 606 TCGA T^CGA
Van91I 528 CCATGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1026 TCTAGA T^CTAGA
XmnI 1165 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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AlwNI 580 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
1511 CAGGGCCTG
BpmI 37 CTGGAG CTGGAG(16/14)
535 CTGGAG
BsmFI 631 GGGAC GGGAC(10/14)
1277 GGGAC
BspLU11I 282 ACATGT A^CATGT
1372 ACATGT
BspMI 584 ACCTGC ACCTGC(4/8)
1327 ACCTGC
Cac8I 1270 GCCCGC GC[ACGT]^[ACGT]GC
1313 GCCAGC
DdeI 887 CTCAG C^T[ACGT]AG
972 CTTAG
DraII 1404 AGGCCCT [GA]G^G[ACGT]CC[CT]
1512 AGGGCCT
EaeI 483 TGGCCA [CT]^GGCC[GA]
1453 CGGCCA
EarI 1044 GAAGAG CTCTTC(1/4)
1165 GAAGAG
EcoO109I 1404 AGGCCCT [GA]G^G[ACGT]CC[CT]
1512 AGGGCCT
FauI 1271 CCCGC CCCGC(4/6)
1086 GCGGG
Ksp632I 1044 GAAGAG CTCTTC(1/4)
1165 GAAGAG
MaeII 428 ACGT A^CGT
1013 ACGT
MspA1I 1077 CCGCTG C[AC]G^C[GT]G
1272 CCGCTG
PleI 84 GAGTC GAGTC(4/5)
369 GAGTC
PstI 380 CTGCAG CTGCA^G
614 CTGCAG
SfcI 380 CTGCAG C^T[GA][CT]AG
614 CTGCAG
Tsp45I 349 GTGAC ^GT[GC]AC
1369 GTCAC
XcmI 659 CCAGACTTTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1134 CCAGCTCAAGGGTGG
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# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AspHI BanII BbvI
BfaI BmyI BsaJI BsiHKAI BsiYI
BslI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cfr13I DpnII DraI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HinfI HphI ItaI MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MvaI NdeII NlaIII NlaIV
NspI RsaI Sau3AI Sau96I ScrFI
SfaNI StyI TfiI Tru9I TseI
Tsp509I TspRI XhoII