Restriction map for pFN21AB4044
 (Circular) (Six-base) MAPSORT of: pFN21AB4044.seq  Check: 736  from: 1  to: 6253



pFN21AB4044  6253 bp

 With 182 enzymes: SgfI * 

                        October 20, 2009 10:54  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2598    2598 
   Size:       6253
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    4063     278 
   Size:         53      83     186     936    2527    2468

  Fragments arranged by size: 

                2527    2468     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3596    1937 
   Size:       1659    4594
 
Acc65I G'GTAC_C

Cuts at:   3574    3574 
   Size:       6253
 
AclI AA'CG_TT

Cuts at:   1563    4380    4753    1563 
   Size:       2817     373    3063

  Fragments arranged by size: 

                3063    2817     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    4316    5700    5968    1632 
   Size:       2684    1384     268    1917

  Fragments arranged by size: 

                2684    1917    1384     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    6056
 
AflIII A'CryG_T

Cuts at:   1203    2287    2433    3377    5152    6129    1203 
   Size:       1084     146     944    1775     977    1327

  Fragments arranged by size: 

                1775    1327    1084     977     944     146
 
AgeI A'CCGG_T

Cuts at:   5084    5084 
   Size:       6253
 
AhdI GACnn_n'nnGTC

Cuts at:   4982    4982 
   Size:       6253
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       6253
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       6253
 
AlwNI CAG_nnn'CTG

Cuts at:   2590    3521    3996    5568    2590 
   Size:        931     475    1572    3275

  Fragments arranged by size: 

                3275    1572     931     475
 
ApaLI G'TGCA_C

Cuts at:   3445    4310    5466    3445 
   Size:        865    1156    4232

  Fragments arranged by size: 

                4232    1156     865
 
ApoI r'AATT_y

Cuts at:   1493    2313    2861    2996    3562    3788    3940    6049    1493 
   Size:        820     548     135     566     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     820     566     548     226     152     135
            
 
AseI AT'TA_AT

Cuts at:    160    1032    2097    4807     160 
   Size:        872    1065    2710    1606

  Fragments arranged by size: 

                2710    1606    1065     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6253
 
AvaI C'yCGr_G

Cuts at:   1870    1943    3578    1870 
   Size:         73    1635    4545

  Fragments arranged by size: 

                4545    1635      73
 
AvrII C'CTAG_G

Cuts at:   3470    3470 
   Size:       6253
 
BamHI G'GATC_C

Cuts at:   2545    3583    3950    2545 
   Size:       1038     367    4848

  Fragments arranged by size: 

                4848    1038     367
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    3574    5029     618 
   Size:        325     217     627    1787    1455    1842

  Fragments arranged by size: 

                1842    1787    1455     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2752    3277    3509    3572    1391 
   Size:       1361     525     232      63    4072

  Fragments arranged by size: 

                4072    1361     525     232      63
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    5282
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    1987    2021    4444    4478    1903 
   Size:         34      50      34    2423      34    3678

  Fragments arranged by size: 

                3678    2423      50      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   4145    5355    4145 
   Size:       1210    5043
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       6253
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4864     136 
   Size:        107     122      71     631     605    3192    1525

  Fragments arranged by size: 

                3192    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   2820    6248    2820 
   Size:       3428    2825
 
BlpI GC'TnA_GC

Cuts at:   2977    3670    2977 
   Size:        693    5560
 
Bme1580I G_kGCm'C

Cuts at:   1790    2799    3449    4314    5470    1790 
   Size:       1009     650     865    1156    2573

  Fragments arranged by size: 

                2573    1156    1009     865     650
 
BmgBI CAC'GTC

Cuts at:   1318    2434    1318 
   Size:       1116    5137
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3304    3984    4942 
   Size:        416      53      83     399      24    1887     680     958

Cuts at:   4942     442 
   Size:       1753

  Fragments arranged by size: 

                1887    1753     958     680     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       6253
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2063    2561    4913    1239 
   Size:        824     498    2352    2579

  Fragments arranged by size: 

                2579    2352     824     498
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3128    4378    5243    5541    3128 
   Size:       1250     865     298    3840

  Fragments arranged by size: 

                3840    1250     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4916     882 
   Size:        617    3417    2219

  Fragments arranged by size: 

                3417    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    4044     493 
   Size:        711    2840    2702

  Fragments arranged by size: 

                2840    2702     711
 
BsaBI GATnn'nnATC

Cuts at:   6247    6247 
   Size:       6253
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    4060    4442    5077     275 
   Size:         53      83     186     936    2527     382     635    1451

  Fragments arranged by size: 

                2527    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   4686    5084    5358    5505    5916    4686 
   Size:        398     274     147     411    5023

  Fragments arranged by size: 

                5023     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    6028

  Fragments arranged by size: 

                6028     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2760    1186 
   Size:       1574    4679
 
BseYI C'CCAG_C

Cuts at:   2102    3279    5456    5952    2102 
   Size:       1177    2177     496    2403

  Fragments arranged by size: 

                2403    2177    1177     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2576    1163 
   Size:        573     840    4840

  Fragments arranged by size: 

                4840     840     573
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3703    4464    5492    1540 
   Size:        275     186    1702     761    1028    2301

  Fragments arranged by size: 

                2301    1702    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2366    2517    2752    3449    4314    4399    5470    2366 
   Size:        151     235     697     865      85    1071    3149

  Fragments arranged by size: 

                3149    1071     865     697     235     151      85
 
BsmI GAATG_Cn'

Cuts at:   3443    3764    3857    3443 
   Size:        321      93    5839

  Fragments arranged by size: 

                5839     321      93
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2366    2517    2752    2799    3277    3449    3509 
   Size:        399     576     151     235      47     478     172      60

Cuts at:   3509    3572    4314    4399    5470    1391 
   Size:         63     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     576     478     399     235     172
                 151      85      63      60      47
 
BspEI T'CCGG_A

Cuts at:   5916    5916 
   Size:       6253
 
BspHI T'CATG_A

Cuts at:   4142    5872    4142 
   Size:       1730    4523
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2598    3341    3608     844 
   Size:       1754     743     267    3489

  Fragments arranged by size: 

                3489    1754     743     267
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3091    4140    1394 
   Size:        429    1268    1049    3507

  Fragments arranged by size: 

                3507    1268    1049     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4748    4922      65 
   Size:       1183    3500     174    1396

  Fragments arranged by size: 

                3500    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4897    5084    1700 
   Size:        110    3087     187    2869

  Fragments arranged by size: 

                3087    2869     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       6253
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       6253
 
BssSI C'ACGA_G

Cuts at:   4313    5325    4313 
   Size:       1012    5241
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    3490     794 
   Size:       2696    3557
 
BstBI TT'CG_AA

Cuts at:   2611    5057    2611 
   Size:       2446    3807
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       6253
 
BstYI r'GATC_y

Cuts at:   1972    2545    2556    2820    3583    3950    4335    4352    5804 
   Size:        573      11     264     763     367     385      17    1452

Cuts at:   5804    5890    5902    6248    1972 
   Size:         86      12     346    1977

  Fragments arranged by size: 

                1977    1452     763     573     385     367     346     264
                  86      17      12      11
 
BtgI C'CryG_G

Cuts at:    513    1065    2799     513 
   Size:        552    1734    3967

  Fragments arranged by size: 

                3967    1734     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    5514

  Fragments arranged by size: 

                5514     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2503    3769    4563    4583    2503 
   Size:       1266     794      20    4173

  Fragments arranged by size: 

                4173    1266     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       6253
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    6218
 
DraI TTT'AAA

Cuts at:   2079    2340    2406    3430    3557    2079 
   Size:        261      66    1024     127    4775

  Fragments arranged by size: 

                4775    1024     261     127      66
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    5260     809 
   Size:        507    3944    1802

  Fragments arranged by size: 

                3944    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2488    3458    3700 
   Size:         54    1425      50     275      18     658     970     242

Cuts at:   3700    4589    6218       8 
   Size:        889    1629      43

  Fragments arranged by size: 

                1629    1425     970     889     658     275     242      54
                  50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3700    1537 
   Size:        275    1888    4090

  Fragments arranged by size: 

                4090    1888     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3044    3165    4183    1353 
   Size:       1691     121    1018    3423

  Fragments arranged by size: 

                3423    1691    1018     121
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4840    5214    5360     387 
   Size:       1064     138    3251     374     146    1280

  Fragments arranged by size: 

                3251    1280    1064     374     146     138
 
EcoICRI GAG'CTC

Cuts at:   2750    2750 
   Size:       6253
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2063    2561    4316    4913    5700    5968    1239 
   Size:        393     431     498    1755     597     787     268    1524

  Fragments arranged by size: 

                1755    1524     787     597     498     431     393     268
            
 
EcoO109I rG'GnC_Cy

Cuts at:   3410    3518    3410 
   Size:        108    6145
 
EcoRI G'AATT_C

Cuts at:   3562    3940    3562 
   Size:        378    5875
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2432    2702    2432 
   Size:        270    5983
 
FspI TGC'GCA

Cuts at:   4759    4759 
   Size:       6253
 
HaeII r_GCGC'y

Cuts at:   1678    5400    1678 
   Size:       3722    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    3236    3268    4894    4926    4968 
   Size:         32     135      32    1664      32    1626      32      42

Cuts at:   4968    5000    1373 
   Size:         32    2626

  Fragments arranged by size: 

                2626    1664    1626     135      42      32      32      32
                  32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3022    3597    3843     669 
   Size:        988     281    1084     575     246    3079

  Fragments arranged by size: 

                3079    1084     988     575     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       6253
 
HpaI GTT'AAC

Cuts at:   3843    3843 
   Size:       6253
 
KpnI G_GTAC'C

Cuts at:   3578    3578 
   Size:       6253
 
MfeI C'AATT_G

Cuts at:   3852    3852 
   Size:       6253
 
MluI A'CGCG_T

Cuts at:   6129    6129 
   Size:       6253
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2228    5367    5551    1305 
   Size:        923    3139     184    2007

  Fragments arranged by size: 

                3139    2007     923     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2490      10 
   Size:         54    1425     343     658    3773

  Fragments arranged by size: 

                3773    1425     658     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2286    2307    2427    4211    4570    4729     518 
   Size:        648    1120      21     120    1784     359     159    2042

  Fragments arranged by size: 

                2042    1784    1120     648     359     159     120      21
            
 
MspA1I CmG'CkG

Cuts at:   1606    3084    3279    3669    4346    5494    5739    1606 
   Size:       1478     195     390     677    1148     245    2120

  Fragments arranged by size: 

                2120    1478    1148     677     390     245     195
 
NcoI C'CATG_G

Cuts at:    513    1065    2799     513 
   Size:        552    1734    3967

  Fragments arranged by size: 

                3967    1734     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       6253
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       6253
 
NotI GC'GGCC_GC

Cuts at:   3700    3700 
   Size:       6253
 
NspI r_CATG'y

Cuts at:   1132    1229    2291    2306    3381    3491    3611    5156    1132 
   Size:         97    1062      15    1075     110     120    1545    2229

  Fragments arranged by size: 

                2229    1545    1075    1062     120     110      97      15
            
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       6253
 
PciI A'CATG_T

Cuts at:   2287    3377    5152    2287 
   Size:       1090    1775    3388

  Fragments arranged by size: 

                3388    1775    1090
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2539    1108 
   Size:       1431    4822
 
PmeI GTTT'AAAC

Cuts at:   3557    3557 
   Size:       6253
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4322    4354    5835    5867    4322 
   Size:         32    1481      32    4708

  Fragments arranged by size: 

                4708    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       6253
 
PsiI TTA'TAA

Cuts at:   3823    3823 
   Size:       6253
 
PstI C_TGCA'G

Cuts at:    830    2389    2623    3605     830 
   Size:       1559     234     982    3478

  Fragments arranged by size: 

                3478    1559     982     234
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       6253
 
SacI G_AGCT'C

Cuts at:   2752    2752 
   Size:       6253
 
SalI G'TCGA_C

Cuts at:   1936    3595    1936 
   Size:       1659    4594
 
SbfI CC_TGCA'GG

Cuts at:   3605    3605 
   Size:       6253
 
ScaI AGT'ACT

Cuts at:   4501    6213    4501 
   Size:       1712    4541
 
SfcI C'TryA_G

Cuts at:    826    1045    2385    2619    3601    4736    5417    5608     826 
   Size:        219    1340     234     982    1135     681     191    1471

  Fragments arranged by size: 

                1471    1340    1135     982     681     234     219     191
            
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6253
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       6253
 
SmaI CCC'GGG

Cuts at:   1872    3580    1872 
   Size:       1708    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3143    4357    5258    5520     820 
   Size:        197     926    1200    1214     901     262    1553

  Fragments arranged by size: 

                1553    1214    1200     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    4044     493 
   Size:       3551    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       6253
 
SphI G_CATG'C

Cuts at:   1132    3611    1132 
   Size:       2479    3774
 
SspI AAT'ATT

Cuts at:      5      52    4177       5 
   Size:         47    4125    2081

  Fragments arranged by size: 

                4125    2081      47
 
StuI AGG'CCT

Cuts at:   1517    2171    1517 
   Size:        654    5599
 
StyI C'CwwG_G

Cuts at:    513    1065    2029    2799    2968    3114    3470    3692     513 
   Size:        552     964     770     169     146     356     222    3074

  Fragments arranged by size: 

                3074     964     770     552     356     222     169     146
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    4280    4297    4450    4635    5080     325 
   Size:       1255    2700      17     153     185     445    1498

  Fragments arranged by size: 

                2700    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2135    3063    4499 
   Size:        274      80      33      51    1443     158     928    1436

Cuts at:   4499    6211      96 
   Size:       1712     138

  Fragments arranged by size: 

                1712    1443    1436     928     274     158     138      80
                  51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2629    2661    2699    2731    3428    3460    6213    6245    2629 
   Size:         32      38      32     697      32    2753      32    2637

  Fragments arranged by size: 

                2753    2637     697      38      32      32      32      32
            
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       6253
 
XbaI T'CTAG_A

Cuts at:   3031    3589    3031 
   Size:        558    5695
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2671    3146    2671 
   Size:        475    5778
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       6253
 
XmaI C'CCGG_G

Cuts at:   1870    3578    1870 
   Size:       1708    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3174    4382    3174 
   Size:       1208    5045
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    4061     276 
   Size:         53      83     186     936    2527    2468

  Fragments arranged by size: 

                2527    2468     936     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI    AvrII    BamHI     BanI    BanII     BbsI     BcgI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NcoI     NdeI     NheI     NotI     NspI     PasI
     PciI    PflMI     PmeI     PpiI    PshAI     PsiI     PstI     PvuI
     SacI     SalI     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI

 Enzymes that do not cut: 

     AfeI     AloI     ApaI     AscI     BaeI     BbeI    BbvCI    BfrBI
     BplI   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I   DraIII
    EcoNI    EcoRV     FseI    FspAI     KasI     NaeI     NarI   NgoMIV
     NruI     NsiI     PacI     PfoI     PmlI    PpuMI   PspOMI    PspXI
     PsrI    PvuII    RsrII    SacII    SanDI     SapI    SexAI     SfiI
     SfoI     SrfI     SwaI
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