Restriction map for pFN21AB3919
 (Linear) (Six-base) MAPSORT of: KIBB3919.seq  Check: 8746  from: 1  to: 1737



KIBB3919.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:02  ..

AatII G_ACGT'C

Cuts at:      0     870    1737 
   Size:        870     867
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     232     770    1121    1154    1522    1737 
   Size:        232     538     351      33     368     215

  Fragments arranged by size: 

                 538     368     351     232     215      33
 
AflIII A'CryG_T

Cuts at:      0     116    1003    1737 
   Size:        116     887     734

  Fragments arranged by size: 

                 887     734     116
 
ApaI G_GGCC'C

Cuts at:      0     150    1166    1737 
   Size:        150    1016     571

  Fragments arranged by size: 

                1016     571     150
 
ApoI r'AATT_y

Cuts at:      0     435     814    1406    1542    1655    1664    1737 
   Size:        435     379     592     136     113       9      73

  Fragments arranged by size: 

                 592     435     379     136     113      73       9
 
AvaI C'yCGr_G

Cuts at:      0     503    1737 
   Size:        503    1234
 
BamHI G'GATC_C

Cuts at:      0     556    1737 
   Size:        556    1181
 
BanI G'GyrC_C

Cuts at:      0     643     754    1252    1737 
   Size:        643     111     498     485

  Fragments arranged by size: 

                 643     498     485     111
 
BanII G_rGCy'C

Cuts at:      0     150     535    1166    1737 
   Size:        150     385     631     571

  Fragments arranged by size: 

                 631     571     385     150
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     112    1737 
   Size:        112    1625
 
BbvCI CC'TCA_GC

Cuts at:      0      25    1016    1737 
   Size:         25     991     721

  Fragments arranged by size: 

                 991     721      25
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     220     254    1276    1310    1737 
   Size:        220      34    1022      34     427

  Fragments arranged by size: 

                1022     427     220      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     573    1737 
   Size:        573    1164
 
BfrBI ATG'CAT

Cuts at:      0     792    1737 
   Size:        792     945
 
BglI GCCn_nnn'nGGC

Cuts at:      0     154     163    1737 
   Size:        154       9    1574

  Fragments arranged by size: 

                1574     154       9
 
Bme1580I G_kGCm'C

Cuts at:      0     150    1166    1737 
   Size:        150    1016     571

  Fragments arranged by size: 

                1016     571     150
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     669    1737 
   Size:        669    1068
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     536     802    1310    1325    1737 
   Size:        536     266     508      15     412

  Fragments arranged by size: 

                 536     508     412     266      15
 
Bpu10I CC'TnA_GC

Cuts at:      0      25    1016    1397    1737 
   Size:         25     991     381     340

  Fragments arranged by size: 

                 991     381     340      25
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     136     522     788    1737 
   Size:        136     386     266     949

  Fragments arranged by size: 

                 949     386     266     136
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     554    1737 
   Size:        554    1183
 
BsaBI GATnn'nnATC

Cuts at:      0     561     733    1737 
   Size:        561     172    1004

  Fragments arranged by size: 

                1004     561     172
 
BsaHI Gr'CG_yC

Cuts at:      0      71     867    1737 
   Size:         71     796     870

  Fragments arranged by size: 

                 870     796      71
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     921     951    1737 
   Size:        921      30     786

  Fragments arranged by size: 

                 921     786      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     525    1033    1036    1172    1737 
   Size:        525     508       3     136     565

  Fragments arranged by size: 

                 565     525     508     136       3
 
BseYI C'CCAG_C

Cuts at:      0     143     479    1236    1737 
   Size:        143     336     757     501

  Fragments arranged by size: 

                 757     501     336     143
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     172    1574    1737 
   Size:        172    1402     163

  Fragments arranged by size: 

                1402     172     163
 
BsiHKAI G_wGCw'C

Cuts at:      0     215    1737 
   Size:        215    1522
 
Bsp1286I G_dGCh'C

Cuts at:      0     150     215     535    1166    1737 
   Size:        150      65     320     631     571

  Fragments arranged by size: 

                 631     571     320     150      65
 
BspHI T'CATG_A

Cuts at:      0    1028    1737 
   Size:       1028     709
 
BsrDI GCAATG_nn'

Cuts at:      0     940     999    1737 
   Size:        940      59     738

  Fragments arranged by size: 

                 940     738      59
 
BssSI C'ACGA_G

Cuts at:      0    1571    1737 
   Size:       1571     166
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1342    1737 
   Size:       1342     395
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     619    1737 
   Size:        619    1118
 
BstYI r'GATC_y

Cuts at:      0      77     556     827    1737 
   Size:         77     479     271     910

  Fragments arranged by size: 

                 910     479     271      77
 
Bsu36I CC'TnA_GG

Cuts at:      0    1158    1737 
   Size:       1158     579
 
ClaI AT'CG_AT

Cuts at:      0     686    1737 
   Size:        686    1051
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     597     632    1737 
   Size:        597      35    1105

  Fragments arranged by size: 

                1105     597      35
 
EaeI y'GGCC_r

Cuts at:      0     473    1737 
   Size:        473    1264
 
EarI CTCTTCn'nnn_

Cuts at:      0     818    1445    1737 
   Size:        818     627     292

  Fragments arranged by size: 

                 818     627     292
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     232     536     770     802    1121    1154    1310    1325 
   Size:        232     304     234      32     319      33     156      15

Cuts at:   1325    1522    1737 
   Size:        197     215

  Fragments arranged by size: 

                 319     304     234     232     215     197     156      33
                  32      15
 
EcoO109I rG'GnC_Cy

Cuts at:      0      91     147    1162    1163    1737 
   Size:         91      56    1015       1     574

  Fragments arranged by size: 

                1015     574      91      56       1
 
EcoRV GAT'ATC

Cuts at:      0     935    1737 
   Size:        935     802
 
HaeII r_GCGC'y

Cuts at:      0     169    1737 
   Size:        169    1568
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     831     863    1134    1166    1209    1241    1737 
   Size:        831      32     271      32      43      32     496

  Fragments arranged by size: 

                 831     496     271      43      32      32      32
 
MluI A'CGCG_T

Cuts at:      0     116    1737 
   Size:        116    1621
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     600     843    1737 
   Size:        600     243     894

  Fragments arranged by size: 

                 894     600     243
 
MslI CAynn'nnrTG

Cuts at:      0    1628    1737 
   Size:       1628     109
 
MspA1I CmG'CkG

Cuts at:      0    1330    1737 
   Size:       1330     407
 
NsiI A_TGCA'T

Cuts at:      0     794    1737 
   Size:        794     943
 
NspI r_CATG'y

Cuts at:      0    1007    1737 
   Size:       1007     730
 
PciI A'CATG_T

Cuts at:      0    1003    1737 
   Size:       1003     734
 
PfoI T'CCnGG_A

Cuts at:      0     552    1095    1737 
   Size:        552     543     642

  Fragments arranged by size: 

                 642     552     543
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     331     363    1372    1404    1737 
   Size:        331      32    1009      32     333

  Fragments arranged by size: 

                1009     333     331      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0      91    1737 
   Size:         91    1646
 
PspOMI G'GGCC_C

Cuts at:      0     146    1162    1737 
   Size:        146    1016     575

  Fragments arranged by size: 

                1016     575     146
 
PstI C_TGCA'G

Cuts at:      0     360    1737 
   Size:        360    1377
 
SanDI GG'GwC_CC

Cuts at:      0      91    1737 
   Size:         91    1646
 
SfcI C'TryA_G

Cuts at:      0     356    1737 
   Size:        356    1381
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     154    1737 
   Size:        154    1583
 
SmlI C'TyrA_G

Cuts at:      0     151     537     803    1737 
   Size:        151     386     266     934

  Fragments arranged by size: 

                 934     386     266     151
 
SpeI A'CTAG_T

Cuts at:      0     300    1737 
   Size:        300    1437
 
StyI C'CwwG_G

Cuts at:      0    1115    1737 
   Size:       1115     622
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     207     625    1146    1737 
   Size:        207     418     521     591

  Fragments arranged by size: 

                 591     521     418     207
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     921     953    1737 
   Size:        921      32     784

  Fragments arranged by size: 

                 921     784      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     675    1737 
   Size:        675    1062
 
ZraI GAC'GTC

Cuts at:      0     868    1737 
   Size:        868     869
 

 Enzymes that do cut:

    AatII     AcuI   AflIII     ApaI     ApoI     AvaI    BamHI     BanI
    BanII     BbsI    BbvCI     BcgI    BciVI    BfrBI     BglI Bme1580I
     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI    BsaXI
    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspHI    BsrDI    BssSI
   BstAPI    BstXI    BstYI   Bsu36I     ClaI    CspCI     EaeI     EarI
  Eco57MI EcoO109I    EcoRV    HaeII    Hin4I     MluI     MmeI     MslI
   MspA1I     NsiI     NspI     PciI     PfoI     PpiI    PpuMI   PspOMI
     PstI    SanDI     SfcI     SfiI     SmlI     SpeI     StyI    TaqII
     TstI  Tth111I     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI     BbeI     BclI    BglII     BlpI    BmgBI     BmtI
     BplI    BsaAI    BsaWI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BstBI   BstEII  BstZ17I
     BtgI    BtgZI     BtsI     DraI   DraIII     DrdI     EagI     EciI
  EcoICRI    EcoNI    EcoRI     FalI     FseI     FspI    FspAI   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MscI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     PacI     PasI
    PflMI     PmeI     PmlI    PshAI     PsiI    PspXI     PsrI     PvuI
    PvuII    RsrII     SacI    SacII     SalI     SapI     SbfI     ScaI
    SexAI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SphI     SrfI
     SspI     StuI     SwaI     TatI     XbaI     XcmI     XhoI     XmaI
     XmnI
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