################################## # NO CUT ENZYMES ( 132 enzymes) # ################################## AatII AccII AccIII AclI AcyI AflII AgeI AhdI Aor51HI ApaI AscI Asp700I AspEI AspI AvaI AviII AvrII BalI BamHI BbeI BbrPI BcgI BciVI BclI BcnI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmAI BsmBI BsoBI BspDI BspEI BspHI BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII KasI KspI MfeI MluI MluNI MroI MscI MunI MvnI NaeI NarI NciI NdeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PstI PvuI RcaI RsrII SacII SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I StuI SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### Acc65I 1155 GGTACC G^GTACC AflIII 906 ACATGT A^C[GA][CT]GT Alw44I 2116 GTGCAC G^TGCAC AlwNI 2080 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 889 CCTGAGG CC^T[ACGT]AGG ApaLI 2116 GTGCAC G^TGCAC AseI 1221 ATTAAT AT^TAAT AsnI 1221 ATTAAT AT^TAAT Asp718I 1155 GGTACC G^GTACC BanI 1155 GGTACC G^G[CT][GA]CC BanII 1396 GAGCTC G[GA]GC[CT]^C BbsI 677 GTCTTC GAAGAC(2/6) BbvCI 1189 GCTGAGG CCTCAGC(-5/-2) BglII 2775 AGATCT A^GATCT BpuAI 677 GTCTTC GAAGAC(2/6) BsaBI 949 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmI 1195 GAATGC GAATGC(1/-1) Bsp1407I 352 TGTACA T^GTACA BspLU11I 906 ACATGT A^CATGT BspMI 1617 ACCTGC ACCTGC(4/8) BsrGI 352 TGTACA T^GTACA BstXI 2581 CCAAATCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 889 CCTGAGG CC^T[ACGT]AGG DraI 2769 TTTAAA TTT^AAA DraII 653 GGGACCC [GA]G^G[ACGT]CC[CT] EaeI 1697 TGGCCG [CT]^GGCC[GA] EarI 24 GAAGAG CTCTTC(1/4) Eco81I 889 CCTGAGG CC^T[ACGT]AGG EcoNI 2608 CCTCCTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 653 GGGACCC [GA]G^G[ACGT]CC[CT] EcoRV 1579 GATATC GAT^ATC HapII 1700 CCGG C^CGG HpaI 276 GTTAAC GTT^AAC HpaII 1700 CCGG C^CGG HphI 1682 TCACC GGTGA(8/7) KpnI 1155 GGTACC GGTAC^C Ksp632I 24 GAAGAG CTCTTC(1/4) MaeII 2660 ACGT A^CGT MamI 949 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MspI 1700 CCGG C^CGG PpuMI 653 GGGACCC [GA]G^G[AT]CC[CT] PshBI 1221 ATTAAT AT^TAAT SacI 1396 GAGCTC GAGCT^C SalI 104 GTCGAC G^TCGAC SfcI 2382 CTACAG C^T[GA][CT]AG SmlI 2612 CTCAAG C^T[CT][GA]AG SpeI 58 ACTAGT A^CTAGT SspBI 352 TGTACA T^GTACA ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### AccI 104 GTCGAC GT^[AC][GT]AC 1487 GTCTAC AciI 1507 GCGG CCGC(-3/-1) 1621 GCGG AvaII 654 GGACC G^G[AT]CC 1623 GGTCC BpmI 422 CTCCAG CTGGAG(16/14) 1828 CTGGAG BsgI 992 GTGCAG GTGCAG(16/14) 1105 GTGCAG BsmFI 653 GGGAC GGGAC(10/14) 2647 GGGAC DsaI 1352 CCATGG C^C[GA][CT]GG 2252 CCATGG MspA1I 47 CAGCTG C[AC]G^C[GT]G 1693 CAGCTG NcoI 1352 CCATGG C^CATGG 2252 CCATGG NheI 132 GCTAGC G^CTAGC 2758 GCTAGC NspI 906 ACATGT [GA]CATG^[CT] 2354 ACATGC PvuII 47 CAGCTG CAG^CTG 1693 CAGCTG ScaI 28 AGTACT AGT^ACT 2230 AGTACT ######################################### # 3 OR MORE CUT ENZYMES ( 65 enzymes) # ######################################### AcsI AfaI AluI AlwI ApoI AspHI BbvI BfaI BmyI BsaJI BseRI BsiHKAI BsiYI BslI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII EcoRII EcoT14I Fnu4HI FokI HaeIII HincII HindII HinfI ItaI MaeI MaeIII MboI MboII MflI MnlI MseI MslI MvaI MwoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII