(Circular) (Six-base) MAPSORT of: pFN21AE3436.seq Check: 6200 from: 1 to: 7492 pFN21AE3436 7492 bp With 182 enzymes: SgfI * March 28, 2012 14:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5302 278 Size: 53 83 186 936 3766 2468 Fragments arranged by size: 3766 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2110 3493 4835 1937 Size: 173 1383 1342 4594 Fragments arranged by size: 4594 1383 1342 173 Acc65I G'GTAC_C Cuts at: 3160 4813 3160 Size: 1653 5839 AclI AA'CG_TT Cuts at: 1563 5619 5992 1563 Size: 4056 373 3063 Fragments arranged by size: 4056 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2465 2511 3029 3120 4332 5555 6939 7207 Size: 833 46 518 91 1212 1223 1384 268 Cuts at: 7207 1632 Size: 1917 Fragments arranged by size: 1917 1384 1223 1212 833 518 268 91 46 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7295 AflIII A'CryG_T Cuts at: 1203 2911 6391 7368 1203 Size: 1708 3480 977 1327 Fragments arranged by size: 3480 1708 1327 977 AgeI A'CCGG_T Cuts at: 6323 6323 Size: 7492 AhdI GACnn_n'nnGTC Cuts at: 6221 6221 Size: 7492 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7492 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7492 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3612 3644 3612 Size: 32 7460 AlwNI CAG_nnn'CTG Cuts at: 4090 5235 6807 4090 Size: 1145 1572 4775 Fragments arranged by size: 4775 1572 1145 ApaLI G'TGCA_C Cuts at: 4121 5549 6705 4121 Size: 1428 1156 4908 Fragments arranged by size: 4908 1428 1156 ApoI r'AATT_y Cuts at: 1493 2096 2231 2639 2665 3117 3140 3523 3606 Size: 603 135 408 26 452 23 383 83 Cuts at: 3606 3639 4306 4801 5027 5179 7288 1493 Size: 33 667 495 226 152 2109 1697 Fragments arranged by size: 2109 1697 667 603 495 452 408 383 226 152 135 83 33 26 23 AseI AT'TA_AT Cuts at: 160 1032 3227 6046 160 Size: 872 2195 2819 1606 Fragments arranged by size: 2819 2195 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7492 AvaI C'yCGr_G Cuts at: 1870 1943 4817 1870 Size: 73 2874 4545 Fragments arranged by size: 4545 2874 73 BamHI G'GATC_C Cuts at: 4822 5189 4822 Size: 367 7125 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3160 4813 6268 618 Size: 325 217 627 1373 1653 1455 1842 Fragments arranged by size: 1842 1653 1455 1373 627 325 217 BanII G_rGCy'C Cuts at: 1391 3405 4811 1391 Size: 2014 1406 4072 Fragments arranged by size: 4072 2014 1406 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2675 928 Size: 971 776 5745 Fragments arranged by size: 5745 971 776 BbvCI CC'TCA_GC Cuts at: 3195 3195 Size: 7492 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5683 5717 1903 Size: 34 3746 34 3678 Fragments arranged by size: 3746 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5384 6594 5384 Size: 1210 6282 BclI T'GATC_A Cuts at: 1548 1548 Size: 7492 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6103 136 Size: 107 122 71 631 605 4431 1525 Fragments arranged by size: 4431 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4780 7487 4780 Size: 2707 4785 BlpI GC'TnA_GC Cuts at: 4909 4909 Size: 7492 Bme1580I G_kGCm'C Cuts at: 1790 4125 5553 6709 1790 Size: 2335 1428 1156 2573 Fragments arranged by size: 2573 2335 1428 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7492 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2410 5223 6181 Size: 416 53 83 399 24 993 2813 958 Cuts at: 6181 442 Size: 1753 Fragments arranged by size: 2813 1753 993 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2141 4767 1056 Size: 1085 2626 3781 Fragments arranged by size: 3781 2626 1085 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4488 4488 Size: 7492 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2412 3854 6152 1239 Size: 1173 1442 2298 2579 Fragments arranged by size: 2579 2298 1442 1173 Bpu10I CC'TnA_GC Cuts at: 3195 3538 3195 Size: 343 7149 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4602 5617 6482 6780 4602 Size: 1015 865 298 5314 Fragments arranged by size: 5314 1015 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6155 882 Size: 617 4656 2219 Fragments arranged by size: 4656 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5283 493 Size: 711 4079 2702 Fragments arranged by size: 4079 2702 711 BsaBI GATnn'nnATC Cuts at: 2958 7486 2958 Size: 4528 2964 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5299 5681 6316 275 Size: 53 83 186 936 3766 382 635 1451 Fragments arranged by size: 3766 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5925 6323 6597 6744 7155 5925 Size: 398 274 147 411 6262 Fragments arranged by size: 6262 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7267 Fragments arranged by size: 7267 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2283 3917 4605 1186 Size: 1097 1634 688 4073 Fragments arranged by size: 4073 1634 1097 688 BseYI C'CCAG_C Cuts at: 6695 7191 6695 Size: 496 6996 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3018 3131 1163 Size: 573 1282 113 5524 Fragments arranged by size: 5524 1282 573 113 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4942 5703 6731 1540 Size: 275 186 2941 761 1028 2301 Fragments arranged by size: 2941 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3373 3405 4125 5553 5638 6709 3373 Size: 32 720 1428 85 1071 4156 Fragments arranged by size: 4156 1428 1071 720 85 32 BsmI GAATG_Cn' Cuts at: 3206 5003 5096 3206 Size: 1797 93 5602 Fragments arranged by size: 5602 1797 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3373 3405 4125 4811 5553 5638 6709 Size: 399 1583 32 720 686 742 85 1071 Cuts at: 6709 1391 Size: 2174 Fragments arranged by size: 2174 1583 1071 742 720 686 399 85 32 BspEI T'CCGG_A Cuts at: 7155 7155 Size: 7492 BspHI T'CATG_A Cuts at: 5381 7111 5381 Size: 1730 5762 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3631 4847 844 Size: 2787 1216 3489 Fragments arranged by size: 3489 2787 1216 BsrBI CCG'CTC Cuts at: 1394 1823 5379 1394 Size: 429 3556 3507 Fragments arranged by size: 3556 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2115 2137 4774 5987 6161 65 Size: 1183 867 22 2637 1213 174 1396 Fragments arranged by size: 2637 1396 1213 1183 867 174 22 BsrFI r'CCGG_y Cuts at: 1700 1810 6136 6323 1700 Size: 110 4326 187 2869 Fragments arranged by size: 4326 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2357 96 Size: 2261 5231 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7492 BssSI C'ACGA_G Cuts at: 5552 6564 5552 Size: 1012 6480 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7492 BstBI TT'CG_AA Cuts at: 6296 6296 Size: 7492 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7492 BstXI CCAn_nnnn'nTGG Cuts at: 4593 4593 Size: 7492 BstYI r'GATC_y Cuts at: 1972 2870 3393 4780 4822 5189 5574 5591 7043 Size: 898 523 1387 42 367 385 17 1452 Cuts at: 7043 7129 7141 7487 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1387 898 523 385 367 346 86 42 17 12 Bsu36I CC'TnA_GG Cuts at: 2895 2895 Size: 7492 BtgI C'CryG_G Cuts at: 513 1065 2004 3357 4257 513 Size: 552 939 1353 900 3748 Fragments arranged by size: 3748 1353 939 900 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6753 Fragments arranged by size: 6753 683 56 BtsI GCAGTG_nn' Cuts at: 2521 3005 3988 5008 5802 5822 2521 Size: 484 983 1020 794 20 4191 Fragments arranged by size: 4191 1020 983 794 484 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7492 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7457 DraI TTT'AAA Cuts at: 4776 4796 4776 Size: 20 7472 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6499 809 Size: 507 5183 1802 Fragments arranged by size: 5183 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3702 4939 5828 Size: 54 1425 50 275 18 1872 1237 889 Cuts at: 5828 7457 8 Size: 1629 43 Fragments arranged by size: 1872 1629 1425 1237 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4939 1537 Size: 275 3127 4090 Fragments arranged by size: 4090 3127 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2024 5422 1353 Size: 671 3398 3423 Fragments arranged by size: 3423 3398 671 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6079 6453 6599 387 Size: 1064 138 4490 374 146 1280 Fragments arranged by size: 4490 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3403 3403 Size: 7492 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2412 2465 2511 3029 3120 3854 4332 Size: 393 780 53 46 518 91 734 478 Cuts at: 4332 5555 6152 6939 7207 1239 Size: 1223 597 787 268 1524 Fragments arranged by size: 1524 1223 787 780 734 597 518 478 393 268 91 53 46 EcoNI CCTnn'n_nnAGG Cuts at: 4617 4617 Size: 7492 EcoO109I rG'GnC_Cy Cuts at: 2659 2659 Size: 7492 EcoRI G'AATT_C Cuts at: 4801 5179 4801 Size: 378 7114 EcoRV GAT'ATC Cuts at: 3586 3586 Size: 7492 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2995 3815 2995 Size: 820 6672 FspI TGC'GCA Cuts at: 5998 5998 Size: 7492 HaeII r_GCGC'y Cuts at: 1678 6639 1678 Size: 4961 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3434 3466 4169 4201 6133 Size: 32 135 32 1862 32 703 32 1932 Cuts at: 6133 6165 6207 6239 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1932 1862 703 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2111 2172 2283 2812 4836 5082 Size: 988 281 173 61 111 529 2024 246 Cuts at: 5082 669 Size: 3079 Fragments arranged by size: 3079 2024 988 529 281 246 173 111 61 HindIII A'AGCT_T Cuts at: 748 748 Size: 7492 HpaI GTT'AAC Cuts at: 2283 5082 2283 Size: 2799 4693 KpnI G_GTAC'C Cuts at: 3164 4817 3164 Size: 1653 5839 MfeI C'AATT_G Cuts at: 5091 5091 Size: 7492 MluI A'CGCG_T Cuts at: 7368 7368 Size: 7492 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2299 2829 2963 3446 4387 4652 6606 6790 Size: 994 530 134 483 941 265 1954 184 Cuts at: 6790 1305 Size: 2007 Fragments arranged by size: 2007 1954 994 941 530 483 265 184 134 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5670 Fragments arranged by size: 5670 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2514 3532 4172 4268 4511 5450 5809 Size: 648 1348 1018 640 96 243 939 359 Cuts at: 5809 5968 518 Size: 159 2042 Fragments arranged by size: 2042 1348 1018 939 648 640 359 243 159 96 MspA1I CmG'CkG Cuts at: 1606 2054 3700 4908 5585 6733 6978 1606 Size: 448 1646 1208 677 1148 245 2120 Fragments arranged by size: 2120 1646 1208 1148 677 448 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3357 4257 513 Size: 552 939 1353 900 3748 Fragments arranged by size: 3748 1353 939 900 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7492 NheI G'CTAG_C Cuts at: 1052 2137 4763 1052 Size: 1085 2626 3781 Fragments arranged by size: 3781 2626 1085 NotI GC'GGCC_GC Cuts at: 4939 4939 Size: 7492 NspI r_CATG'y Cuts at: 1132 1229 2915 4363 4850 6395 1132 Size: 97 1686 1448 487 1545 2229 Fragments arranged by size: 2229 1686 1545 1448 487 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7492 PciI A'CATG_T Cuts at: 2911 6391 2911 Size: 3480 4012 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7492 PfoI T'CCnGG_A Cuts at: 3396 3396 Size: 7492 PmeI GTTT'AAAC Cuts at: 4796 4796 Size: 7492 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5561 5593 7074 7106 5561 Size: 32 1481 32 5947 Fragments arranged by size: 5947 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2659 2659 Size: 7492 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7492 PsiI TTA'TAA Cuts at: 2445 5062 2445 Size: 2617 4875 PstI C_TGCA'G Cuts at: 830 4844 830 Size: 4014 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7492 PvuII CAG'CTG Cuts at: 2054 3700 2054 Size: 1646 5846 SacI G_AGCT'C Cuts at: 3405 3405 Size: 7492 SalI G'TCGA_C Cuts at: 1936 2109 4834 1936 Size: 173 2725 4594 Fragments arranged by size: 4594 2725 173 SanDI GG'GwC_CC Cuts at: 2659 2659 Size: 7492 SbfI CC_TGCA'GG Cuts at: 4844 4844 Size: 7492 ScaI AGT'ACT Cuts at: 2035 4237 5740 7452 2035 Size: 2202 1503 1712 2075 Fragments arranged by size: 2202 2075 1712 1503 SfcI C'TryA_G Cuts at: 826 1045 4387 4840 5975 6656 6847 826 Size: 219 3342 453 1135 681 191 1471 Fragments arranged by size: 3342 1471 1135 681 453 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7492 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7492 SmaI CCC'GGG Cuts at: 1872 4819 1872 Size: 2947 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4617 5596 6497 6759 820 Size: 197 926 2674 979 901 262 1553 Fragments arranged by size: 2674 1553 979 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5283 493 Size: 4790 2702 SpeI A'CTAG_T Cuts at: 152 2063 152 Size: 1911 5581 SphI G_CATG'C Cuts at: 1132 4850 1132 Size: 3718 3774 SspI AAT'ATT Cuts at: 5 52 2699 3014 3922 5416 5 Size: 47 2647 315 908 1494 2081 Fragments arranged by size: 2647 2081 1494 908 315 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7492 StyI C'CwwG_G Cuts at: 513 1065 2004 3357 3615 4257 4931 513 Size: 552 939 1353 258 642 674 3074 Fragments arranged by size: 3074 1353 939 674 642 552 258 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5519 5536 5689 5874 6319 325 Size: 1255 3939 17 153 185 445 1498 Fragments arranged by size: 3939 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2033 2357 3574 Size: 274 80 33 51 1443 56 324 1217 Cuts at: 3574 3659 4235 5738 7450 96 Size: 85 576 1503 1712 138 Fragments arranged by size: 1712 1503 1443 1217 576 324 274 138 85 80 56 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4234 4266 7452 7484 4234 Size: 32 3186 32 4242 Fragments arranged by size: 4242 3186 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7492 XbaI T'CTAG_A Cuts at: 4828 4828 Size: 7492 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2344 3697 4423 2344 Size: 1353 726 5413 Fragments arranged by size: 5413 1353 726 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7492 XmaI C'CCGG_G Cuts at: 1870 4817 1870 Size: 2947 4545 XmnI GAAnn'nnTTC Cuts at: 5621 5621 Size: 7492 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5300 276 Size: 53 83 186 936 3766 2468 Fragments arranged by size: 3766 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I DraIII FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI