Restriction map for
pFN21AB3650
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# NO CUT ENZYMES ( 128 enzymes) #
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AatII AccIII AclI AcsI AflII
AflIII AgeI Alw44I AlwI Aor51HI
ApaLI ApoI AscI AseI AsnI
AspI AvaI AviII AvrII BamHI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BsaBI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspEI BspHI
BspLU11I BspMI BsrDI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstYI BstZ17I CelII CpoI DpnII
DraI DrdI EagI EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KspI MaeI MamI
MboI MfeI MflI MluI MroI
MseI MunI NdeI NdeII NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmeI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacII SalI
SapI Sau3AI SbfI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tru9I Tsp509I Tth111I XbaI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccI 811 GTCTAC GT^[AC][GT]AC
AhdI 58 GACCAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1418 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 474 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 58 GACCAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 124 GAGCTC G[AT]GC[AT]^C
BbeI 382 GGCGCC GGCGC^C
BbrPI 1197 CACGTG CAC^GTG
BbvCI 439 CCTCAGC CCTCAGC(-5/-2)
BglI 31 GCCCTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 1197 CACGTG [CT]AC^GT[GA]
BsiHKAI 124 GAGCTC G[AT]GC[AT]^C
BspDI 616 ATCGAT AT^CGAT
BsrBI 805 GAGCGG CCGCTC(-3/-3)
BsrFI 1181 GCCGGC [GA]^CCGG[CT]
BstXI 1152 CCACAGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 1181 GCCGGC [GA]^CCGG[CT]
ClaI 616 ATCGAT AT^CGAT
DraIII 1018 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 58 GACCAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 660 CCTCTTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
KasI 382 GGCGCC G^GCGCC
MslI 1153 CACAGCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 1181 GCCGGC GCC^GGC
NarI 382 GGCGCC GG^CGCC
NcoI 1046 CCATGG C^CATGG
NgoMIV 1181 GCCGGC G^CCGGC
PflMI 1417 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1197 CACGTG CAC^GTG
PmlI 1197 CACGTG CAC^GTG
PvuII 933 CAGCTG CAG^CTG
SacI 124 GAGCTC GAGCT^C
SfoI 382 GGCGCC GGC^GCC
TfiI 324 GAATC G^A[AT]TC
Van91I 1417 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 786 CCATAGCTACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 474 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 27 enzymes) #
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Acc65I 572 GGTACC G^GTACC
982 GGTACC
AccII 190 CGCG CG^CG
1192 CGCG
AcyI 382 GGCGCC G[GA]^CG[CT]C
484 GACGCC
ApaI 282 GGGCCC GGGCC^C
609 GGGCCC
Asp718I 572 GGTACC G^GTACC
982 GGTACC
AvaII 705 GGACC G^G[AT]CC
1371 GGACC
BalI 1094 TGGCCA TGG^CCA
1425 TGGCCA
BbsI 651 GAAGAC GAAGAC(2/6)
1306 GTCTTC
BpuAI 651 GAAGAC GAAGAC(2/6)
1306 GTCTTC
BsaHI 382 GGCGCC G[GA]^CG[CT]C
484 GACGCC
BsmFI 929 GGGAC GGGAC(10/14)
1010 GGGAC
BstAPI 40 GCATGGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1017 GCACGGTGTGC
BstUI 190 CGCG CG^CG
1192 CGCG
DsaI 1046 CCATGG C^C[GA][CT]GG
1158 CCGTGG
HaeII 382 GGCGCC [GA]GCGC^[CT]
683 GGCGCT
HgaI 484 GACGC GACGC(5/10)
1039 GACGC
Hin1I 382 GGCGCC G[GA]^CG[CT]C
484 GACGCC
HphI 491 TCACC GGTGA(8/7)
629 TCACC
KpnI 572 GGTACC GGTAC^C
982 GGTACC
MaeIII 119 GTGAC ^GT[ACGT]AC
184 GTGAC
MluNI 1094 TGGCCA TGG^CCA
1425 TGGCCA
MscI 1094 TGGCCA TGG^CCA
1425 TGGCCA
MvnI 190 CGCG CG^CG
1192 CGCG
SfcI 792 CTACAG C^T[GA][CT]AG
1257 CTACAG
SmlI 408 CTCAAG C^T[CT][GA]AG
499 CTCAAG
StuI 412 AGGCCT AGG^CCT
565 AGGCCT
Tsp45I 119 GTGAC ^GT[GC]AC
184 GTGAC
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# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AfaI AluI AocI BanI
BanII BbvI BcnI BmyI BpmI
BsaI BsaJI BseRI BsgI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI Bsu36I Cac8I CfoI
Cfr13I DdeI DraII EaeI EarI
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI Ksp632I
MaeII MboII MnlI MspA1I MspI
MvaI MwoI NciI NlaIII NlaIV
PleI RsaI Sau96I ScaI ScrFI
SfaNI StyI TaqI TseI TspRI
TthHB8I