(Circular) (Six-base) MAPSORT of: pFN21AB3650.seq Check: 6361 from: 1 to: 6139 pFN21AB3650 6139 bp With 182 enzymes: SgfI * October 20, 2009 10:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3949 278 Size: 53 83 186 936 2413 2468 Fragments arranged by size: 2468 2413 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2817 3482 1937 Size: 880 665 4594 Fragments arranged by size: 4594 880 665 Acc65I G'GTAC_C Cuts at: 2577 2987 3460 2577 Size: 410 473 5256 Fragments arranged by size: 5256 473 410 AclI AA'CG_TT Cuts at: 1563 4266 4639 1563 Size: 2703 373 3063 Fragments arranged by size: 3063 2703 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4202 5586 5854 1632 Size: 2570 1384 268 1917 Fragments arranged by size: 2570 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5942 AflIII A'CryG_T Cuts at: 1203 5038 6015 1203 Size: 3835 977 1327 Fragments arranged by size: 3835 1327 977 AgeI A'CCGG_T Cuts at: 4970 4970 Size: 6139 AhdI GACnn_n'nnGTC Cuts at: 2068 4868 2068 Size: 2800 3339 AleI CACnn'nnGTG Cuts at: 1166 3162 1166 Size: 1996 4143 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6139 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2837 2869 2837 Size: 32 6107 AlwNI CAG_nnn'CTG Cuts at: 3428 3882 5454 3428 Size: 454 1572 4113 Fragments arranged by size: 4113 1572 454 ApaI G_GGCC'C Cuts at: 2291 2618 2291 Size: 327 5812 ApaLI G'TGCA_C Cuts at: 4196 5352 4196 Size: 1156 4983 ApoI r'AATT_y Cuts at: 1493 3448 3674 3826 5935 1493 Size: 1955 226 152 2109 1697 Fragments arranged by size: 2109 1955 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4693 160 Size: 872 3661 1606 Fragments arranged by size: 3661 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6139 AvaI C'yCGr_G Cuts at: 1870 1943 3464 1870 Size: 73 1521 4545 Fragments arranged by size: 4545 1521 73 BamHI G'GATC_C Cuts at: 3469 3836 3469 Size: 367 5772 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2008 2387 2577 2987 3082 Size: 325 217 627 221 379 190 410 95 Cuts at: 3082 3460 4915 618 Size: 378 1455 1842 Fragments arranged by size: 1842 1455 627 410 379 378 325 221 217 190 95 BanII G_rGCy'C Cuts at: 1391 2133 2291 2618 3458 1391 Size: 742 158 327 840 4072 Fragments arranged by size: 4072 840 742 327 158 BbeI G_GCGC'C Cuts at: 2391 2391 Size: 6139 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2663 3304 928 Size: 971 764 641 3763 Fragments arranged by size: 3763 971 764 641 BbvCI CC'TCA_GC Cuts at: 2445 2445 Size: 6139 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4330 4364 1903 Size: 34 2393 34 3678 Fragments arranged by size: 3678 2393 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4031 5241 4031 Size: 1210 4929 BclI T'GATC_A Cuts at: 1548 1548 Size: 6139 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2042 4750 136 Size: 107 122 71 631 605 370 2708 1525 Fragments arranged by size: 2708 1525 631 605 370 122 107 71 BglII A'GATC_T Cuts at: 6134 6134 Size: 6139 BlpI GC'TnA_GC Cuts at: 3556 3556 Size: 6139 Bme1580I G_kGCm'C Cuts at: 1790 2291 2618 4200 5356 1790 Size: 501 327 1582 1156 2573 Fragments arranged by size: 2573 1582 1156 501 327 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6139 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2311 2851 2862 Size: 416 53 83 399 24 894 540 11 Cuts at: 2862 2995 3870 4828 442 Size: 133 875 958 1753 Fragments arranged by size: 1753 958 894 875 540 416 399 133 83 53 24 11 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6139 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2152 2152 Size: 6139 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2294 3205 3345 4799 1239 Size: 1055 911 140 1454 2579 Fragments arranged by size: 2579 1454 1055 911 140 Bpu10I CC'TnA_GC Cuts at: 2334 2445 2334 Size: 111 6028 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2398 2489 4264 5129 5427 2398 Size: 91 1775 865 298 3110 Fragments arranged by size: 3110 1775 865 298 91 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2011 2417 2434 4802 882 Size: 617 512 406 17 2368 2219 Fragments arranged by size: 2368 2219 617 512 406 17 BsaAI yAC'GTr Cuts at: 493 1204 3204 3930 493 Size: 711 2000 726 2702 Fragments arranged by size: 2702 2000 726 711 BsaBI GATnn'nnATC Cuts at: 6133 6133 Size: 6139 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2388 2490 3946 4328 Size: 53 83 186 936 855 102 1456 382 Cuts at: 4328 4963 275 Size: 635 1451 Fragments arranged by size: 1456 1451 936 855 635 382 186 102 83 53 BsaWI w'CCGG_w Cuts at: 4572 4970 5244 5391 5802 4572 Size: 398 274 147 411 4909 Fragments arranged by size: 4909 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2210 2240 2453 2483 2837 Size: 30 165 30 807 30 213 30 354 Cuts at: 2837 2867 3164 3194 3377 3407 1178 Size: 30 297 30 183 30 3910 Fragments arranged by size: 3910 807 354 297 213 183 165 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2264 2474 2477 2891 3242 1186 Size: 1078 210 3 414 351 4083 Fragments arranged by size: 4083 1078 414 351 210 3 BseYI C'CCAG_C Cuts at: 2452 2940 3412 3421 5342 5838 2452 Size: 488 472 9 1921 496 2753 Fragments arranged by size: 2753 1921 496 488 472 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2071 2140 2491 1163 Size: 573 335 69 351 4811 Fragments arranged by size: 4811 573 351 335 69 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3589 4350 5378 1540 Size: 275 186 1588 761 1028 2301 Fragments arranged by size: 2301 1588 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2133 4200 4285 5356 2133 Size: 2067 85 1071 2916 Fragments arranged by size: 2916 2067 1071 85 BsmI GAATG_Cn' Cuts at: 3650 3743 3650 Size: 93 6046 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2133 2291 2618 3458 4200 4285 5356 Size: 399 343 158 327 840 742 85 1071 Cuts at: 5356 1391 Size: 2174 Fragments arranged by size: 2174 1071 840 742 399 343 327 158 85 BspEI T'CCGG_A Cuts at: 5802 5802 Size: 6139 BspHI T'CATG_A Cuts at: 4028 5758 4028 Size: 1730 4409 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3494 844 Size: 2650 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2812 4026 1394 Size: 429 989 1214 3507 Fragments arranged by size: 3507 1214 989 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4634 4808 65 Size: 1183 3386 174 1396 Fragments arranged by size: 3386 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3186 4783 4970 1700 Size: 110 1376 1597 187 2869 Fragments arranged by size: 2869 1597 1376 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6139 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6139 BssSI C'ACGA_G Cuts at: 4199 5211 4199 Size: 1012 5127 BstAPI GCAn_nnn'nTGC Cuts at: 794 2051 3028 794 Size: 1257 977 3905 Fragments arranged by size: 3905 1257 977 BstBI TT'CG_AA Cuts at: 4943 4943 Size: 6139 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6139 BstXI CCAn_nnnn'nTGG Cuts at: 3164 3164 Size: 6139 BstYI r'GATC_y Cuts at: 1972 3469 3836 4221 4238 5690 5776 5788 6134 Size: 1497 367 385 17 1452 86 12 346 Cuts at: 6134 1972 Size: 1977 Fragments arranged by size: 1977 1497 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2358 2379 2400 2358 Size: 21 21 6097 Fragments arranged by size: 6097 21 21 BtgI C'CryG_G Cuts at: 513 1065 2004 3051 3163 513 Size: 552 939 1047 112 3489 Fragments arranged by size: 3489 1047 939 552 112 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3107 3382 487 Size: 683 56 1881 275 3244 Fragments arranged by size: 3244 1881 683 275 56 BtsI GCAGTG_nn' Cuts at: 2479 2571 3655 4449 4469 2479 Size: 92 1084 794 20 4149 Fragments arranged by size: 4149 1084 794 92 20 ClaI AT'CG_AT Cuts at: 1554 2622 1554 Size: 1068 5071 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6104 DraI TTT'AAA Cuts at: 3443 3443 Size: 6139 DraIII CAC_nnn'GTG Cuts at: 3028 3028 Size: 6139 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5146 809 Size: 507 3830 1802 Fragments arranged by size: 3830 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2803 3058 3099 Size: 54 1425 50 275 18 973 255 41 Cuts at: 3099 3166 3188 3430 3586 4475 6104 8 Size: 67 22 242 156 889 1629 43 Fragments arranged by size: 1629 1425 973 889 275 255 242 156 67 54 50 43 41 22 18 EagI C'GGCC_G Cuts at: 1537 1812 3586 1537 Size: 275 1774 4090 Fragments arranged by size: 4090 1774 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2358 2672 2969 4069 1353 Size: 1005 314 297 1100 3423 Fragments arranged by size: 3423 1100 1005 314 297 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4726 5100 5246 387 Size: 1064 138 3137 374 146 1280 Fragments arranged by size: 3137 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2131 2131 Size: 6139 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2294 3205 3345 4202 4799 5586 5854 Size: 393 662 911 140 857 597 787 268 Cuts at: 5854 1239 Size: 1524 Fragments arranged by size: 1524 911 857 787 662 597 393 268 140 EcoNI CCTnn'n_nnAGG Cuts at: 2669 2669 Size: 6139 EcoO109I rG'GnC_Cy Cuts at: 2287 2288 2368 2614 2615 2287 Size: 1 80 246 1 5811 Fragments arranged by size: 5811 246 80 1 1 EcoRI G'AATT_C Cuts at: 3448 3826 3448 Size: 378 5761 FspI TGC'GCA Cuts at: 4645 4645 Size: 6139 HaeII r_GCGC'y Cuts at: 1678 2391 2692 5286 1678 Size: 713 301 2594 2531 Fragments arranged by size: 2594 2531 713 301 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2010 2042 2054 2086 2416 Size: 32 135 32 438 32 12 32 330 Cuts at: 2416 2448 4780 4812 4854 4886 1373 Size: 32 2332 32 42 32 2626 Fragments arranged by size: 2626 2332 438 330 135 42 32 32 32 32 32 32 32 12 HincII GTy'rAC Cuts at: 669 1657 1938 3483 3729 669 Size: 988 281 1545 246 3079 Fragments arranged by size: 3079 1545 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6139 HpaI GTT'AAC Cuts at: 3729 3729 Size: 6139 KasI G'GCGC_C Cuts at: 2387 2387 Size: 6139 KpnI G_GTAC'C Cuts at: 2581 2991 3464 2581 Size: 410 473 5256 Fragments arranged by size: 5256 473 410 MfeI C'AATT_G Cuts at: 3738 3738 Size: 6139 MluI A'CGCG_T Cuts at: 6015 6015 Size: 6139 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5253 5437 1305 Size: 3948 184 2007 Fragments arranged by size: 3948 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3101 3432 10 Size: 54 1425 343 1269 331 2717 Fragments arranged by size: 2717 1425 1269 343 331 54 MslI CAynn'nnrTG Cuts at: 518 1166 3162 4097 4456 4615 518 Size: 648 1996 935 359 159 2042 Fragments arranged by size: 2042 1996 935 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2085 2932 2940 3555 4232 5380 5625 1606 Size: 479 847 8 615 677 1148 245 2120 Fragments arranged by size: 2120 1148 847 677 615 479 245 8 NaeI GCC'GGC Cuts at: 3188 3188 Size: 6139 NarI GG'CG_CC Cuts at: 2388 2388 Size: 6139 NcoI C'CATG_G Cuts at: 513 1065 2004 3051 513 Size: 552 939 1047 3601 Fragments arranged by size: 3601 1047 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6139 NgoMIV G'CCGG_C Cuts at: 3186 3186 Size: 6139 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6139 NotI GC'GGCC_GC Cuts at: 3586 3586 Size: 6139 NspI r_CATG'y Cuts at: 1132 1229 3497 5042 1132 Size: 97 2268 1545 2229 Fragments arranged by size: 2268 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6139 PciI A'CATG_T Cuts at: 5038 5038 Size: 6139 PflMI CCAn_nnn'nTGG Cuts at: 1108 3428 1108 Size: 2320 3819 PfoI T'CCnGG_A Cuts at: 2706 2754 2706 Size: 48 6091 PmeI GTTT'AAAC Cuts at: 3443 3443 Size: 6139 PmlI CAC'GTG Cuts at: 3204 3204 Size: 6139 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2837 2869 4208 4240 5721 5753 2837 Size: 32 1339 32 1481 32 3223 Fragments arranged by size: 3223 1481 1339 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6139 PsiI TTA'TAA Cuts at: 3709 3709 Size: 6139 PspOMI G'GGCC_C Cuts at: 2287 2614 2287 Size: 327 5812 PstI C_TGCA'G Cuts at: 830 3491 830 Size: 2661 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6139 PvuII CAG'CTG Cuts at: 2940 2940 Size: 6139 SacI G_AGCT'C Cuts at: 2133 2133 Size: 6139 SalI G'TCGA_C Cuts at: 1936 3481 1936 Size: 1545 4594 SbfI CC_TGCA'GG Cuts at: 3491 3491 Size: 6139 ScaI AGT'ACT Cuts at: 2540 2684 2840 3335 3407 4387 6099 2540 Size: 144 156 495 72 980 1712 2580 Fragments arranged by size: 2580 1712 980 495 156 144 72 SfcI C'TryA_G Cuts at: 826 1045 2797 3262 3487 4622 5303 5494 826 Size: 219 1752 465 225 1135 681 191 1471 Fragments arranged by size: 1752 1471 1135 681 465 225 219 191 SfoI GGC'GCC Cuts at: 2389 2389 Size: 6139 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6139 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6139 SmaI CCC'GGG Cuts at: 1872 3466 1872 Size: 1594 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2413 2504 4243 5144 5406 820 Size: 197 926 470 91 1739 901 262 1553 Fragments arranged by size: 1739 1553 926 901 470 262 197 91 SnaBI TAC'GTA Cuts at: 493 3930 493 Size: 3437 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6139 SphI G_CATG'C Cuts at: 1132 3497 1132 Size: 2365 3774 SspI AAT'ATT Cuts at: 5 52 4063 5 Size: 47 4011 2081 Fragments arranged by size: 4011 2081 47 StuI AGG'CCT Cuts at: 1517 2419 2572 1517 Size: 902 153 5084 Fragments arranged by size: 5084 902 153 StyI C'CwwG_G Cuts at: 513 1065 2004 2775 3051 3096 3578 513 Size: 552 939 771 276 45 482 3074 Fragments arranged by size: 3074 939 771 552 482 276 45 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4166 4183 4336 4521 4966 325 Size: 1255 2586 17 153 185 445 1498 Fragments arranged by size: 2586 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2226 2538 2682 Size: 274 80 33 51 1443 249 312 144 Cuts at: 2682 2838 3333 3405 4385 6097 96 Size: 156 495 72 980 1712 138 Fragments arranged by size: 1712 1443 980 495 312 274 249 156 144 138 80 72 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2099 2131 2453 2485 6099 6131 2099 Size: 32 322 32 3614 32 2107 Fragments arranged by size: 3614 2107 322 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6139 XbaI T'CTAG_A Cuts at: 3475 3475 Size: 6139 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2798 2798 Size: 6139 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6139 XmaI C'CCGG_G Cuts at: 1870 3464 1870 Size: 1594 4545 XmnI GAAnn'nnTTC Cuts at: 2483 4268 2483 Size: 1785 4354 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3947 276 Size: 53 83 186 936 2413 2468 Fragments arranged by size: 2468 2413 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I EcoRV FalI FseI FspAI NruI NsiI PacI PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI