(Circular) (Six-base) MAPSORT of: pFN21AB3488.seq Check: 5908 from: 1 to: 5755 pFN21AB3488 5755 bp With 182 enzymes: SgfI * October 20, 2009 10:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3565 278 Size: 53 83 186 936 2029 2468 Fragments arranged by size: 2468 2029 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3098 1937 Size: 1161 4594 Acc65I G'GTAC_C Cuts at: 3076 3076 Size: 5755 AclI AA'CG_TT Cuts at: 1563 2616 3882 4255 1563 Size: 1053 1266 373 3063 Fragments arranged by size: 3063 1266 1053 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3044 3818 5202 5470 1632 Size: 1412 774 1384 268 1917 Fragments arranged by size: 1917 1412 1384 774 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5558 AflIII A'CryG_T Cuts at: 1203 4654 5631 1203 Size: 3451 977 1327 Fragments arranged by size: 3451 1327 977 AgeI A'CCGG_T Cuts at: 4586 4586 Size: 5755 AhdI GACnn_n'nnGTC Cuts at: 4484 4484 Size: 5755 AleI CACnn'nnGTG Cuts at: 1166 2214 1166 Size: 1048 4707 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5755 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2554 2586 2554 Size: 32 5723 AlwNI CAG_nnn'CTG Cuts at: 2930 3498 5070 2930 Size: 568 1572 3615 Fragments arranged by size: 3615 1572 568 ApaLI G'TGCA_C Cuts at: 3812 4968 3812 Size: 1156 4599 ApoI r'AATT_y Cuts at: 1493 2347 3064 3290 3442 5551 1493 Size: 854 717 226 152 2109 1697 Fragments arranged by size: 2109 1697 854 717 226 152 AseI AT'TA_AT Cuts at: 160 1032 2484 4309 160 Size: 872 1452 1825 1606 Fragments arranged by size: 1825 1606 1452 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5755 AvaI C'yCGr_G Cuts at: 1870 1943 3080 1870 Size: 73 1137 4545 Fragments arranged by size: 4545 1137 73 AvrII C'CTAG_G Cuts at: 2578 2578 Size: 5755 BamHI G'GATC_C Cuts at: 3085 3452 3085 Size: 367 5388 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3076 4531 618 Size: 325 217 627 1289 1455 1842 Fragments arranged by size: 1842 1455 1289 627 325 217 BanII G_rGCy'C Cuts at: 1391 3074 1391 Size: 1683 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4784 BbvCI CC'TCA_GC Cuts at: 2999 2999 Size: 5755 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2222 2256 3946 3980 1903 Size: 34 285 34 1690 34 3678 Fragments arranged by size: 3678 1690 285 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3647 4857 3647 Size: 1210 4545 BclI T'GATC_A Cuts at: 1548 2781 2796 1548 Size: 1233 15 4507 Fragments arranged by size: 4507 1233 15 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4366 136 Size: 107 122 71 631 605 2694 1525 Fragments arranged by size: 2694 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5750 5750 Size: 5755 BlpI GC'TnA_GC Cuts at: 3172 3172 Size: 5755 Bme1580I G_kGCm'C Cuts at: 1790 3816 4972 1790 Size: 2026 1156 2573 Fragments arranged by size: 2573 2026 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5755 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3486 4444 442 Size: 416 53 83 399 24 2069 958 1753 Fragments arranged by size: 2069 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5755 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2436 2907 2952 4415 1239 Size: 1197 471 45 1463 2579 Fragments arranged by size: 2579 1463 1197 471 45 Bpu10I CC'TnA_GC Cuts at: 2999 2999 Size: 5755 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3880 4745 5043 3880 Size: 865 298 4592 Fragments arranged by size: 4592 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4418 882 Size: 617 2919 2219 Fragments arranged by size: 2919 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3546 493 Size: 711 2342 2702 Fragments arranged by size: 2702 2342 711 BsaBI GATnn'nnATC Cuts at: 5749 5749 Size: 5755 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3562 3944 4579 275 Size: 53 83 186 936 2029 382 635 1451 Fragments arranged by size: 2029 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4188 4586 4860 5007 5418 4188 Size: 398 274 147 411 4525 Fragments arranged by size: 4525 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2051 2081 2799 2829 1178 Size: 30 165 30 648 30 718 30 4104 Fragments arranged by size: 4104 718 648 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5755 BseYI C'CCAG_C Cuts at: 2142 2909 4958 5454 2142 Size: 767 2049 496 2443 Fragments arranged by size: 2443 2049 767 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2627 1163 Size: 573 891 4291 Fragments arranged by size: 4291 891 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3205 3966 4994 1540 Size: 275 186 1204 761 1028 2301 Fragments arranged by size: 2301 1204 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3816 3901 4972 3816 Size: 85 1071 4599 Fragments arranged by size: 4599 1071 85 BsmI GAATG_Cn' Cuts at: 2568 2628 3266 3359 2568 Size: 60 638 93 4964 Fragments arranged by size: 4964 638 93 60 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3074 3816 3901 4972 1391 Size: 399 1284 742 85 1071 2174 Fragments arranged by size: 2174 1284 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5418 5418 Size: 5755 BspHI T'CATG_A Cuts at: 3644 5374 3644 Size: 1730 4025 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2756 3110 844 Size: 1912 354 3489 Fragments arranged by size: 3489 1912 354 BsrBI CCG'CTC Cuts at: 1394 1823 3642 1394 Size: 429 1819 3507 Fragments arranged by size: 3507 1819 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2446 2628 2640 2964 4250 4424 65 Size: 1183 1198 182 12 324 1286 174 1396 Fragments arranged by size: 1396 1286 1198 1183 324 182 174 12 BsrFI r'CCGG_y Cuts at: 1700 1810 2524 4399 4586 1700 Size: 110 714 1875 187 2869 Fragments arranged by size: 2869 1875 714 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5755 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5755 BssSI C'ACGA_G Cuts at: 3815 4827 3815 Size: 1012 4743 BstAPI GCAn_nnn'nTGC Cuts at: 794 2161 794 Size: 1367 4388 BstBI TT'CG_AA Cuts at: 2104 4559 2104 Size: 2455 3300 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5755 BstXI CCAn_nnnn'nTGG Cuts at: 2953 2953 Size: 5755 BstYI r'GATC_y Cuts at: 1972 2433 3085 3452 3837 3854 5306 5392 5404 Size: 461 652 367 385 17 1452 86 12 Cuts at: 5404 5750 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 652 461 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2191 2191 Size: 5755 BtgI C'CryG_G Cuts at: 513 1065 2004 2534 513 Size: 552 939 530 3734 Fragments arranged by size: 3734 939 552 530 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5016 Fragments arranged by size: 5016 683 56 BtsI GCAGTG_nn' Cuts at: 3271 4065 4085 3271 Size: 794 20 4941 Fragments arranged by size: 4941 794 20 ClaI AT'CG_AT Cuts at: 1554 2701 1554 Size: 1147 4608 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5720 DraI TTT'AAA Cuts at: 3059 3059 Size: 5755 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4762 809 Size: 507 3446 1802 Fragments arranged by size: 3446 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2204 3202 4091 Size: 54 1425 50 275 18 374 998 889 Cuts at: 4091 5720 8 Size: 1629 43 Fragments arranged by size: 1629 1425 998 889 374 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3202 1537 Size: 275 1390 4090 Fragments arranged by size: 4090 1390 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2172 2412 3003 3685 1353 Size: 819 240 591 682 3423 Fragments arranged by size: 3423 819 682 591 240 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4342 4716 4862 387 Size: 1064 138 2753 374 146 1280 Fragments arranged by size: 2753 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2436 2907 2952 3044 3818 4415 5202 Size: 393 804 471 45 92 774 597 787 Cuts at: 5202 5470 1239 Size: 268 1524 Fragments arranged by size: 1524 804 787 774 597 471 393 268 92 45 EcoRI G'AATT_C Cuts at: 3064 3442 3064 Size: 378 5377 FspI TGC'GCA Cuts at: 4261 4261 Size: 5755 HaeII r_GCGC'y Cuts at: 1678 4902 1678 Size: 3224 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2799 2831 4396 4428 4470 Size: 32 135 32 1227 32 1565 32 42 Cuts at: 4470 4502 1373 Size: 32 2626 Fragments arranged by size: 2626 1565 1227 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3099 3345 669 Size: 988 281 1161 246 3079 Fragments arranged by size: 3079 1161 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5755 HpaI GTT'AAC Cuts at: 3345 3345 Size: 5755 KpnI G_GTAC'C Cuts at: 3080 3080 Size: 5755 MfeI C'AATT_G Cuts at: 3354 3354 Size: 5755 MluI A'CGCG_T Cuts at: 5631 5631 Size: 5755 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2597 2599 2854 4869 5053 1305 Size: 1292 2 255 2015 184 2007 Fragments arranged by size: 2015 2007 1292 255 184 2 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3933 Fragments arranged by size: 3933 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2214 2253 2421 2633 2649 2822 2951 Size: 648 1048 39 168 212 16 173 129 Cuts at: 2951 3713 4072 4231 518 Size: 762 359 159 2042 Fragments arranged by size: 2042 1048 762 648 359 212 173 168 159 129 39 16 MspA1I CmG'CkG Cuts at: 1606 2064 2279 3171 3848 4996 5241 1606 Size: 458 215 892 677 1148 245 2120 Fragments arranged by size: 2120 1148 892 677 458 245 215 NcoI C'CATG_G Cuts at: 513 1065 2004 2534 513 Size: 552 939 530 3734 Fragments arranged by size: 3734 939 552 530 NdeI CA'TA_TG Cuts at: 387 387 Size: 5755 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5755 NotI GC'GGCC_GC Cuts at: 3202 3202 Size: 5755 NspI r_CATG'y Cuts at: 1132 1229 3113 4658 1132 Size: 97 1884 1545 2229 Fragments arranged by size: 2229 1884 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5755 PciI A'CATG_T Cuts at: 4654 4654 Size: 5755 PflMI CCAn_nnn'nTGG Cuts at: 1108 2930 1108 Size: 1822 3933 PfoI T'CCnGG_A Cuts at: 2668 2929 2668 Size: 261 5494 PmeI GTTT'AAAC Cuts at: 3059 3059 Size: 5755 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3824 3856 5337 5369 3824 Size: 32 1481 32 4210 Fragments arranged by size: 4210 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5755 PsiI TTA'TAA Cuts at: 3325 3325 Size: 5755 PstI C_TGCA'G Cuts at: 830 2052 2284 2767 3107 830 Size: 1222 232 483 340 3478 Fragments arranged by size: 3478 1222 483 340 232 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5755 PvuII CAG'CTG Cuts at: 2064 2279 2064 Size: 215 5540 SalI G'TCGA_C Cuts at: 1936 3097 1936 Size: 1161 4594 SapI GCTCTTCn'nnn_ Cuts at: 3003 3003 Size: 5755 SbfI CC_TGCA'GG Cuts at: 3107 3107 Size: 5755 ScaI AGT'ACT Cuts at: 4003 5715 4003 Size: 1712 4043 SfcI C'TryA_G Cuts at: 826 1045 2048 2280 2763 3103 4238 4919 5110 Size: 219 1003 232 483 340 1135 681 191 Cuts at: 5110 826 Size: 1471 Fragments arranged by size: 1471 1135 1003 681 483 340 232 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5755 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5755 SmaI CCC'GGG Cuts at: 1872 3082 1872 Size: 1210 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3859 4760 5022 820 Size: 197 926 1916 901 262 1553 Fragments arranged by size: 1916 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3546 493 Size: 3053 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5755 SphI G_CATG'C Cuts at: 1132 3113 1132 Size: 1981 3774 SspI AAT'ATT Cuts at: 5 52 3679 5 Size: 47 3627 2081 Fragments arranged by size: 3627 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5755 StyI C'CwwG_G Cuts at: 513 1065 2004 2534 2578 3194 513 Size: 552 939 530 44 616 3074 Fragments arranged by size: 3074 939 616 552 530 44 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2173 3782 3799 3952 4137 4582 325 Size: 1255 593 1609 17 153 185 445 1498 Fragments arranged by size: 1609 1498 1255 593 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4001 5713 96 Size: 274 80 33 51 1443 2024 1712 138 Fragments arranged by size: 2024 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2051 2083 2911 2943 5715 5747 2051 Size: 32 828 32 2772 32 2059 Fragments arranged by size: 2772 2059 828 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5755 XbaI T'CTAG_A Cuts at: 3091 3091 Size: 5755 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2541 2866 2541 Size: 325 5430 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5755 XmaI C'CCGG_G Cuts at: 1870 3080 1870 Size: 1210 4545 XmnI GAAnn'nnTTC Cuts at: 3884 3884 Size: 5755 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3563 276 Size: 53 83 186 936 2029 2468 Fragments arranged by size: 2468 2029 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI