(Circular) (Six-base) MAPSORT of: pFN21AB3450.seq Check: 5883 from: 1 to: 5584 pFN21AB3450 5584 bp With 182 enzymes: SgfI * October 20, 2009 10:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3394 278 Size: 53 83 186 936 1858 2468 Fragments arranged by size: 2468 1858 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2927 1937 Size: 990 4594 Acc65I G'GTAC_C Cuts at: 2905 2905 Size: 5584 AclI AA'CG_TT Cuts at: 1563 3711 4084 1563 Size: 2148 373 3063 Fragments arranged by size: 3063 2148 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3647 5031 5299 1632 Size: 2015 1384 268 1917 Fragments arranged by size: 2015 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5387 AflIII A'CryG_T Cuts at: 1203 4483 5460 1203 Size: 3280 977 1327 Fragments arranged by size: 3280 1327 977 AgeI A'CCGG_T Cuts at: 4415 4415 Size: 5584 AhdI GACnn_n'nnGTC Cuts at: 4313 4313 Size: 5584 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5584 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2370 1184 Size: 1186 4398 AlwNI CAG_nnn'CTG Cuts at: 3327 4899 3327 Size: 1572 4012 ApaI G_GGCC'C Cuts at: 2037 2045 2037 Size: 8 5576 ApaLI G'TGCA_C Cuts at: 3641 4797 3641 Size: 1156 4428 ApoI r'AATT_y Cuts at: 1493 2893 3119 3271 5380 1493 Size: 1400 226 152 2109 1697 Fragments arranged by size: 2109 1697 1400 226 152 AseI AT'TA_AT Cuts at: 160 1032 4138 160 Size: 872 3106 1606 Fragments arranged by size: 3106 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5584 AvaI C'yCGr_G Cuts at: 1870 1943 2036 2682 2909 1870 Size: 73 93 646 227 4545 Fragments arranged by size: 4545 646 227 93 73 BamHI G'GATC_C Cuts at: 2914 3281 2914 Size: 367 5217 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2905 4360 618 Size: 325 217 627 1118 1455 1842 Fragments arranged by size: 1842 1455 1118 627 325 217 BanII G_rGCy'C Cuts at: 1391 2037 2045 2155 2903 1391 Size: 646 8 110 748 4072 Fragments arranged by size: 4072 748 646 110 8 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4613 BbvCI CC'TCA_GC Cuts at: 2677 2677 Size: 5584 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2596 2630 3775 3809 1903 Size: 34 659 34 1145 34 3678 Fragments arranged by size: 3678 1145 659 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3476 4686 3476 Size: 1210 4374 BclI T'GATC_A Cuts at: 1548 1548 Size: 5584 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2049 2568 4195 Size: 107 122 71 631 605 377 519 1627 Cuts at: 4195 136 Size: 1525 Fragments arranged by size: 1627 1525 631 605 519 377 122 107 71 BglII A'GATC_T Cuts at: 2244 5579 2244 Size: 3335 2249 BlpI GC'TnA_GC Cuts at: 3001 3001 Size: 5584 Bme1580I G_kGCm'C Cuts at: 1790 2037 2045 2609 3645 4801 1790 Size: 247 8 564 1036 1156 2573 Fragments arranged by size: 2573 1156 1036 564 247 8 BmgBI CAC'GTC Cuts at: 1318 2413 1318 Size: 1095 4489 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2521 3315 4273 Size: 416 53 83 399 24 1104 794 958 Cuts at: 4273 442 Size: 1753 Fragments arranged by size: 1753 1104 958 794 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5584 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2258 2850 4244 1239 Size: 1019 592 1394 2579 Fragments arranged by size: 2579 1394 1019 592 Bpu10I CC'TnA_GC Cuts at: 2677 2677 Size: 5584 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2294 3709 4574 4872 2294 Size: 1415 865 298 3006 Fragments arranged by size: 3006 1415 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4247 882 Size: 617 2748 2219 Fragments arranged by size: 2748 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3375 493 Size: 711 2171 2702 Fragments arranged by size: 2702 2171 711 BsaBI GATnn'nnATC Cuts at: 5578 5578 Size: 5584 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2016 3391 3773 4408 Size: 53 83 186 936 483 1375 382 635 Cuts at: 4408 275 Size: 1451 Fragments arranged by size: 1451 1375 936 635 483 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4017 4415 4689 4836 5247 4017 Size: 398 274 147 411 4354 Fragments arranged by size: 4354 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2779 2809 2848 2878 1178 Size: 30 165 30 1376 30 39 30 3884 Fragments arranged by size: 3884 1376 165 39 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2255 1186 Size: 1069 4515 BseYI C'CCAG_C Cuts at: 4787 5283 4787 Size: 496 5088 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2043 2360 2580 2674 2870 1163 Size: 573 307 317 220 94 196 3877 Fragments arranged by size: 3877 573 317 307 220 196 94 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2122 3034 3795 4823 1540 Size: 275 186 121 912 761 1028 2301 Fragments arranged by size: 2301 1028 912 761 275 186 121 BsiHKAI G_wGCw'C Cuts at: 2155 2435 3645 3730 4801 2155 Size: 280 1210 85 1071 2938 Fragments arranged by size: 2938 1210 1071 280 85 BsmI GAATG_Cn' Cuts at: 3095 3188 3095 Size: 93 5491 BsmBI CGTCTCn'nnnn_ Cuts at: 2623 2623 Size: 5584 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2037 2045 2155 2435 2609 2903 3645 Size: 399 247 8 110 280 174 294 742 Cuts at: 3645 3730 4801 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 294 280 247 174 110 85 8 BspEI T'CCGG_A Cuts at: 5247 5247 Size: 5584 BspHI T'CATG_A Cuts at: 2646 3473 5203 2646 Size: 827 1730 3027 Fragments arranged by size: 3027 1730 827 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2939 844 Size: 2095 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3471 1394 Size: 429 1648 3507 Fragments arranged by size: 3507 1648 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4079 4253 65 Size: 1183 2831 174 1396 Fragments arranged by size: 2831 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2018 2357 4228 4415 1700 Size: 110 208 339 1871 187 2869 Fragments arranged by size: 2869 1871 339 208 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5584 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5584 BssSI C'ACGA_G Cuts at: 3644 4656 3644 Size: 1012 4572 BstAPI GCAn_nnn'nTGC Cuts at: 794 2387 794 Size: 1593 3991 BstBI TT'CG_AA Cuts at: 4388 4388 Size: 5584 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5584 BstXI CCAn_nnnn'nTGG Cuts at: 2443 2473 2443 Size: 30 5554 BstYI r'GATC_y Cuts at: 1972 2244 2914 3281 3666 3683 5135 5221 5233 Size: 272 670 367 385 17 1452 86 12 Cuts at: 5233 5579 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 670 385 367 346 272 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2068 2080 2122 2146 2458 513 Size: 552 939 64 12 42 24 312 3639 Fragments arranged by size: 3639 939 552 312 64 42 24 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4845 Fragments arranged by size: 4845 683 56 BtsI GCAGTG_nn' Cuts at: 2101 2691 3100 3894 3914 2101 Size: 590 409 794 20 3771 Fragments arranged by size: 3771 794 590 409 20 ClaI AT'CG_AT Cuts at: 1554 2661 1554 Size: 1107 4477 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5549 DraI TTT'AAA Cuts at: 2888 2888 Size: 5584 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2822 4591 809 Size: 507 1506 1769 1802 Fragments arranged by size: 1802 1769 1506 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2119 2359 2407 Size: 54 1425 50 275 18 289 240 48 Cuts at: 2407 2445 2475 3031 3920 5549 8 Size: 38 30 556 889 1629 43 Fragments arranged by size: 1629 1425 889 556 289 275 240 54 50 48 43 38 30 18 EagI C'GGCC_G Cuts at: 1537 1812 2119 3031 1537 Size: 275 307 912 4090 Fragments arranged by size: 4090 912 307 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2258 3514 1353 Size: 905 1256 3423 Fragments arranged by size: 3423 1256 905 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 4171 4545 4691 387 Size: 1064 138 435 2147 374 146 1280 Fragments arranged by size: 2147 1280 1064 435 374 146 138 EcoICRI GAG'CTC Cuts at: 2153 2153 Size: 5584 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2258 2850 3647 4244 5031 5299 1239 Size: 393 626 592 797 597 787 268 1524 Fragments arranged by size: 1524 797 787 626 597 592 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2771 2771 Size: 5584 EcoO109I rG'GnC_Cy Cuts at: 2033 2041 2042 2301 2033 Size: 8 1 259 5316 Fragments arranged by size: 5316 259 8 1 EcoRI G'AATT_C Cuts at: 2893 3271 2893 Size: 378 5206 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2740 2740 Size: 5584 FspI TGC'GCA Cuts at: 2501 4090 2501 Size: 1589 3995 HaeII r_GCGC'y Cuts at: 1678 4731 1678 Size: 3053 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2848 2880 4225 4257 4299 Size: 32 135 32 1276 32 1345 32 42 Cuts at: 4299 4331 1373 Size: 32 2626 Fragments arranged by size: 2626 1345 1276 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2928 3174 669 Size: 988 281 990 246 3079 Fragments arranged by size: 3079 990 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5584 HpaI GTT'AAC Cuts at: 3174 3174 Size: 5584 KpnI G_GTAC'C Cuts at: 2909 2909 Size: 5584 MfeI C'AATT_G Cuts at: 3183 3183 Size: 5584 MluI A'CGCG_T Cuts at: 5460 5460 Size: 5584 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4698 4882 1305 Size: 3393 184 2007 Fragments arranged by size: 3393 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2409 2447 2477 10 Size: 54 1425 343 577 38 30 3117 Fragments arranged by size: 3117 1425 577 343 54 38 30 MslI CAynn'nnrTG Cuts at: 518 1166 3542 3901 4060 518 Size: 648 2376 359 159 2042 Fragments arranged by size: 2376 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2027 2070 2082 2148 2565 2854 2870 3000 Size: 421 43 12 66 417 289 16 130 Cuts at: 3000 3677 4825 5070 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 421 417 289 245 130 66 43 16 12 NaeI GCC'GGC Cuts at: 2359 2359 Size: 5584 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4093 Fragments arranged by size: 4093 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5584 NgoMIV G'CCGG_C Cuts at: 2357 2357 Size: 5584 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5584 NotI GC'GGCC_GC Cuts at: 3031 3031 Size: 5584 NspI r_CATG'y Cuts at: 1132 1229 2942 4487 1132 Size: 97 1713 1545 2229 Fragments arranged by size: 2229 1713 1545 97 PasI CC'CwG_GG Cuts at: 1348 2054 2601 2701 1348 Size: 706 547 100 4231 Fragments arranged by size: 4231 706 547 100 PciI A'CATG_T Cuts at: 4483 4483 Size: 5584 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5584 PmeI GTTT'AAAC Cuts at: 2888 2888 Size: 5584 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3653 3685 5166 5198 3653 Size: 32 1481 32 4039 Fragments arranged by size: 4039 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5584 PsiI TTA'TAA Cuts at: 3154 3154 Size: 5584 PspOMI G'GGCC_C Cuts at: 2033 2041 2033 Size: 8 5576 PstI C_TGCA'G Cuts at: 830 2936 830 Size: 2106 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5584 PvuII CAG'CTG Cuts at: 2870 2870 Size: 5584 SacI G_AGCT'C Cuts at: 2155 2155 Size: 5584 SacII CC_GC'GG Cuts at: 2071 2083 2149 2071 Size: 12 66 5506 Fragments arranged by size: 5506 66 12 SalI G'TCGA_C Cuts at: 1936 2926 1936 Size: 990 4594 SbfI CC_TGCA'GG Cuts at: 2936 2936 Size: 5584 ScaI AGT'ACT Cuts at: 3832 5544 3832 Size: 1712 3872 SexAI A'CCwGG_T Cuts at: 2369 2369 Size: 5584 SfcI C'TryA_G Cuts at: 826 1045 2746 2932 4067 4748 4939 826 Size: 219 1701 186 1135 681 191 1471 Fragments arranged by size: 1701 1471 1135 681 219 191 186 SfiI GGCCn_nnn'nGGCC Cuts at: 2049 2049 Size: 5584 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5584 SgrAI Cr'CCGG_yG Cuts at: 1700 2018 1700 Size: 318 5266 SmaI CCC'GGG Cuts at: 1872 2038 2684 2911 1872 Size: 166 646 227 4545 Fragments arranged by size: 4545 646 227 166 SmlI C'TyrA_G Cuts at: 820 1017 1943 2309 3688 4589 4851 820 Size: 197 926 366 1379 901 262 1553 Fragments arranged by size: 1553 1379 926 901 366 262 197 SnaBI TAC'GTA Cuts at: 493 3375 493 Size: 2882 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5584 SphI G_CATG'C Cuts at: 1132 2942 1132 Size: 1810 3774 SspI AAT'ATT Cuts at: 5 52 3508 5 Size: 47 3456 2081 Fragments arranged by size: 3456 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5584 StyI C'CwwG_G Cuts at: 513 1065 2004 2436 2715 3023 513 Size: 552 939 432 279 308 3074 Fragments arranged by size: 3074 939 552 432 308 279 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2405 2411 2614 3611 3628 3781 3966 Size: 1255 825 6 203 997 17 153 185 Cuts at: 3966 4411 325 Size: 445 1498 Fragments arranged by size: 1498 1255 997 825 445 203 185 153 17 6 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3830 5542 96 Size: 274 80 33 51 1443 1853 1712 138 Fragments arranged by size: 1853 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2056 2088 2165 2197 2779 2811 5544 5576 2056 Size: 32 77 32 582 32 2733 32 2064 Fragments arranged by size: 2733 2064 582 77 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5584 XbaI T'CTAG_A Cuts at: 2920 2920 Size: 5584 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5584 XmaI C'CCGG_G Cuts at: 1870 2036 2682 2909 1870 Size: 166 646 227 4545 Fragments arranged by size: 4545 646 227 166 XmnI GAAnn'nnTTC Cuts at: 3713 3713 Size: 5584 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3392 276 Size: 53 83 186 936 1858 2468 Fragments arranged by size: 2468 1858 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SanDI SapI SfoI SrfI SwaI XcmI