(Circular) (Six-base) MAPSORT of: pFN21AB3432.seq Check: 4995 from: 1 to: 5047 pFN21AB3432 5047 bp With 182 enzymes: SgfI * October 20, 2009 10:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2857 278 Size: 53 83 186 936 1321 2468 Fragments arranged by size: 2468 1321 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2390 1937 Size: 453 4594 Acc65I G'GTAC_C Cuts at: 2368 2368 Size: 5047 AclI AA'CG_TT Cuts at: 1563 3174 3547 1563 Size: 1611 373 3063 Fragments arranged by size: 3063 1611 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3110 4494 4762 1632 Size: 1478 1384 268 1917 Fragments arranged by size: 1917 1478 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4850 AflIII A'CryG_T Cuts at: 1203 3946 4923 1203 Size: 2743 977 1327 Fragments arranged by size: 2743 1327 977 AgeI A'CCGG_T Cuts at: 3878 3878 Size: 5047 AhdI GACnn_n'nnGTC Cuts at: 3776 3776 Size: 5047 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5047 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5047 AlwNI CAG_nnn'CTG Cuts at: 2219 2790 4362 2219 Size: 571 1572 2904 Fragments arranged by size: 2904 1572 571 ApaLI G'TGCA_C Cuts at: 3104 4260 3104 Size: 1156 3891 ApoI r'AATT_y Cuts at: 1493 2356 2582 2734 4843 1493 Size: 863 226 152 2109 1697 Fragments arranged by size: 2109 1697 863 226 152 AseI AT'TA_AT Cuts at: 160 1032 3601 160 Size: 872 2569 1606 Fragments arranged by size: 2569 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5047 AvaI C'yCGr_G Cuts at: 1870 1943 2372 1870 Size: 73 429 4545 Fragments arranged by size: 4545 429 73 BamHI G'GATC_C Cuts at: 2377 2744 2377 Size: 367 4680 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2368 3823 618 Size: 325 217 627 581 1455 1842 Fragments arranged by size: 1842 1455 627 581 325 217 BanII G_rGCy'C Cuts at: 1391 2025 2366 1391 Size: 634 341 4072 Fragments arranged by size: 4072 634 341 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4076 BbvCI CC'TCA_GC Cuts at: 2271 2271 Size: 5047 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3238 3272 1903 Size: 34 1301 34 3678 Fragments arranged by size: 3678 1301 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2939 4149 2939 Size: 1210 3837 BclI T'GATC_A Cuts at: 1548 1548 Size: 5047 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2131 3658 136 Size: 107 122 71 631 605 459 1527 1525 Fragments arranged by size: 1527 1525 631 605 459 122 107 71 BglII A'GATC_T Cuts at: 5042 5042 Size: 5047 BlpI GC'TnA_GC Cuts at: 2464 2464 Size: 5047 Bme1580I G_kGCm'C Cuts at: 1790 3108 4264 1790 Size: 1318 1156 2573 Fragments arranged by size: 2573 1318 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5047 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2778 3736 442 Size: 416 53 83 399 24 1361 958 1753 Fragments arranged by size: 1753 1361 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5047 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2196 2250 3707 1239 Size: 957 54 1457 2579 Fragments arranged by size: 2579 1457 957 54 Bpu10I CC'TnA_GC Cuts at: 2271 2271 Size: 5047 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3172 4037 4335 3172 Size: 865 298 3884 Fragments arranged by size: 3884 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3710 882 Size: 617 2211 2219 Fragments arranged by size: 2219 2211 617 BsaAI yAC'GTr Cuts at: 493 1204 2838 493 Size: 711 1634 2702 Fragments arranged by size: 2702 1634 711 BsaBI GATnn'nnATC Cuts at: 5041 5041 Size: 5047 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2854 3236 3871 275 Size: 53 83 186 936 1321 382 635 1451 Fragments arranged by size: 1451 1321 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3480 3878 4152 4299 4710 3480 Size: 398 274 147 411 3817 Fragments arranged by size: 3817 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4822 Fragments arranged by size: 4822 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2288 2324 1186 Size: 1102 36 3909 Fragments arranged by size: 3909 1102 36 BseYI C'CCAG_C Cuts at: 4250 4746 4250 Size: 496 4551 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2036 2211 1163 Size: 573 300 175 3999 Fragments arranged by size: 3999 573 300 175 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2497 3258 4286 1540 Size: 275 186 496 761 1028 2301 Fragments arranged by size: 2301 1028 761 496 275 186 BsiHKAI G_wGCw'C Cuts at: 2025 3108 3193 4264 2025 Size: 1083 85 1071 2808 Fragments arranged by size: 2808 1083 1071 85 BsmI GAATG_Cn' Cuts at: 2558 2651 2558 Size: 93 4954 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2025 2366 3108 3193 4264 1391 Size: 399 235 341 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 341 235 85 BspEI T'CCGG_A Cuts at: 4710 4710 Size: 5047 BspHI T'CATG_A Cuts at: 2936 4666 2936 Size: 1730 3317 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2215 2402 844 Size: 1371 187 3489 Fragments arranged by size: 3489 1371 187 BsrBI CCG'CTC Cuts at: 1394 1823 2934 1394 Size: 429 1111 3507 Fragments arranged by size: 3507 1111 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2194 3542 3716 65 Size: 1183 946 1348 174 1396 Fragments arranged by size: 1396 1348 1183 946 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2108 2198 3691 3878 1700 Size: 110 298 90 1493 187 2869 Fragments arranged by size: 2869 1493 298 187 110 90 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5047 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5047 BssSI C'ACGA_G Cuts at: 3107 4119 3107 Size: 1012 4035 BstAPI GCAn_nnn'nTGC Cuts at: 794 2222 794 Size: 1428 3619 BstBI TT'CG_AA Cuts at: 3851 3851 Size: 5047 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5047 BstYI r'GATC_y Cuts at: 1972 2377 2744 3129 3146 4598 4684 4696 5042 Size: 405 367 385 17 1452 86 12 346 Cuts at: 5042 1972 Size: 1977 Fragments arranged by size: 1977 1452 405 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3556 Fragments arranged by size: 3556 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4308 Fragments arranged by size: 4308 683 56 BtsI GCAGTG_nn' Cuts at: 2253 2563 3357 3377 2253 Size: 310 794 20 3923 Fragments arranged by size: 3923 794 310 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5047 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5012 DraI TTT'AAA Cuts at: 2351 2351 Size: 5047 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4054 809 Size: 507 2738 1802 Fragments arranged by size: 2738 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2157 2494 3383 Size: 54 1425 50 275 18 327 337 889 Cuts at: 3383 5012 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 337 327 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2494 1537 Size: 275 682 4090 Fragments arranged by size: 4090 682 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2977 1353 Size: 1624 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3634 4008 4154 387 Size: 1064 138 2045 374 146 1280 Fragments arranged by size: 2045 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2023 2023 Size: 5047 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2196 2250 3110 3707 4494 4762 1239 Size: 393 564 54 860 597 787 268 1524 Fragments arranged by size: 1524 860 787 597 564 393 268 54 EcoO109I rG'GnC_Cy Cuts at: 2152 2240 2152 Size: 88 4959 EcoRI G'AATT_C Cuts at: 2356 2734 2356 Size: 378 4669 FspI TGC'GCA Cuts at: 3553 3553 Size: 5047 HaeII r_GCGC'y Cuts at: 1678 4194 1678 Size: 2516 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3688 3720 3762 3794 1373 Size: 32 135 32 2116 32 42 32 2626 Fragments arranged by size: 2626 2116 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2167 2391 2637 669 Size: 988 281 229 224 246 3079 Fragments arranged by size: 3079 988 281 246 229 224 HindIII A'AGCT_T Cuts at: 748 748 Size: 5047 HpaI GTT'AAC Cuts at: 2637 2637 Size: 5047 KpnI G_GTAC'C Cuts at: 2372 2372 Size: 5047 MfeI C'AATT_G Cuts at: 2646 2646 Size: 5047 MluI A'CGCG_T Cuts at: 4923 4923 Size: 5047 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4161 4345 1305 Size: 2856 184 2007 Fragments arranged by size: 2856 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2159 10 Size: 54 1425 343 327 2898 Fragments arranged by size: 2898 1425 343 327 54 MslI CAynn'nnrTG Cuts at: 518 1166 2058 3005 3364 3523 518 Size: 648 892 947 359 159 2042 Fragments arranged by size: 2042 947 892 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2219 2270 2463 3140 4288 4533 1606 Size: 613 51 193 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 613 245 193 51 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3556 Fragments arranged by size: 3556 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5047 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5047 NotI GC'GGCC_GC Cuts at: 2494 2494 Size: 5047 NspI r_CATG'y Cuts at: 1132 1229 2405 3950 1132 Size: 97 1176 1545 2229 Fragments arranged by size: 2229 1545 1176 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5047 PciI A'CATG_T Cuts at: 3946 3946 Size: 5047 PflMI CCAn_nnn'nTGG Cuts at: 1108 2219 1108 Size: 1111 3936 PmeI GTTT'AAAC Cuts at: 2351 2351 Size: 5047 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3116 3148 4629 4661 3116 Size: 32 1481 32 3502 Fragments arranged by size: 3502 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2240 2240 Size: 5047 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5047 PsiI TTA'TAA Cuts at: 2617 2617 Size: 5047 PstI C_TGCA'G Cuts at: 830 2399 830 Size: 1569 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5047 PvuII CAG'CTG Cuts at: 2219 2270 2219 Size: 51 4996 SacI G_AGCT'C Cuts at: 2025 2025 Size: 5047 SalI G'TCGA_C Cuts at: 1936 2389 1936 Size: 453 4594 SbfI CC_TGCA'GG Cuts at: 2399 2399 Size: 5047 ScaI AGT'ACT Cuts at: 3295 5007 3295 Size: 1712 3335 SfcI C'TryA_G Cuts at: 826 1045 2395 3530 4211 4402 826 Size: 219 1350 1135 681 191 1471 Fragments arranged by size: 1471 1350 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5047 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5047 SmaI CCC'GGG Cuts at: 1872 2374 1872 Size: 502 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3151 4052 4314 820 Size: 197 926 1208 901 262 1553 Fragments arranged by size: 1553 1208 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2838 493 Size: 2345 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5047 SphI G_CATG'C Cuts at: 1132 2405 1132 Size: 1273 3774 SspI AAT'ATT Cuts at: 5 52 2971 5 Size: 47 2919 2081 Fragments arranged by size: 2919 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5047 StyI C'CwwG_G Cuts at: 513 1065 2004 2486 513 Size: 552 939 482 3074 Fragments arranged by size: 3074 939 552 482 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3074 3091 3244 3429 3874 325 Size: 1255 1494 17 153 185 445 1498 Fragments arranged by size: 1498 1494 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3293 5005 96 Size: 274 80 33 51 1443 1316 1712 138 Fragments arranged by size: 1712 1443 1316 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5007 5039 5007 Size: 32 5015 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5047 XbaI T'CTAG_A Cuts at: 2383 2383 Size: 5047 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5047 XmaI C'CCGG_G Cuts at: 1870 2372 1870 Size: 502 4545 XmnI GAAnn'nnTTC Cuts at: 3176 3176 Size: 5047 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2855 276 Size: 53 83 186 936 1321 2468 Fragments arranged by size: 2468 1321 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI