Restriction map for
pFN21AB3440
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# NO CUT ENZYMES ( 153 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AcyI AflII AgeI AhdI Alw44I
AocI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII BalI BamHI BanI
BbeI BbsI BbvCI BcgI BciVI
BclI BfrI BglI BglII BlpI
Bpu1102I BpuAI BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DdeI
DraI DraIII DrdI EaeI EagI
Eam1105I EarI EclXI Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
Ksp632I KspI MamI MfeI MluNI
MroI MscI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmeI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccI 589 GTCTAC GT^[AC][GT]AC
AccII 135 CGCG CG^CG
AfaI 258 GTAC GT^AC
AflIII 134 ACGCGT A^C[GA][CT]GT
AlwNI 547 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 152 AGCGCT AGC^GCT
AspHI 607 GAGCAC G[AT]GC[AT]^C
AvrII 370 CCTAGG C^CTAGG
BbrPI 433 CACGTG CAC^GTG
BfaI 371 CTAG C^TAG
BlnI 370 CCTAGG C^CTAGG
BsaAI 433 CACGTG [CT]AC^GT[GA]
BsgI 315 GTGCAG GTGCAG(16/14)
BsiHKAI 607 GAGCAC G[AT]GC[AT]^C
BsmAI 533 GAGAC GTCTC(1/5)
BstUI 135 CGCG CG^CG
DsaI 635 CCGTGG C^C[GA][CT]GG
Eco47III 152 AGCGCT AGC^GCT
EcoNI 108 CCTTTTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 370 CCTAGG C^C[AT][AT]GG
MaeI 371 CTAG C^TAG
MboII 731 TCTTC GAAGA(8/7)
MluI 134 ACGCGT A^CGCGT
MseI 140 TTAA T^TAA
MspA1I 497 CAGCTG C[AC]G^C[GT]G
MvnI 135 CGCG CG^CG
PflMI 546 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 362 GAGTC GAGTC(4/5)
PmaCI 433 CACGTG CAC^GTG
PmlI 433 CACGTG CAC^GTG
PvuII 497 CAGCTG CAG^CTG
RsaI 258 GTAC GT^AC
ScaI 257 AGTACT AGT^ACT
StyI 370 CCTAGG C^C[AT][AT]GG
TaqI 200 TCGA T^CGA
TfiI 658 GATTC G^A[AT]TC
Tru9I 140 TTAA T^TAA
Tsp509I 103 AATT ^AATT
TthHB8I 200 TCGA T^CGA
Van91I 546 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 23 enzymes) #
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ApaI 340 GGGCCC GGGCC^C
400 GGGCCC
AvaII 78 GGTCC G^G[AT]CC
539 GGACC
BsmFI 117 GGGAC GGGAC(10/14)
145 GGGAC
BsmI 127 GAATGC GAATGC(1/-1)
458 GCATTC
BsrI 260 ACTGG ACTGG(1/-1)
536 ACTGG
BstF5I 279 CATCC GGATG(2/0)
311 GGATG
BstYI 548 AGATCC [GA]^GATC[CT]
766 GGATCT
Cac8I 294 GCGGGC GC[ACGT]^[ACGT]GC
338 GCGGGC
DraII 399 GGGGCCC [GA]G^G[ACGT]CC[CT]
400 GGGCCCT
EcoO109I 399 GGGGCCC [GA]G^G[ACGT]CC[CT]
400 GGGCCCT
FauI 294 GCGGG CCCGC(4/6)
338 GCGGG
FokI 279 CATCC GGATG(9/13)
311 GGATG
HaeII 152 AGCGCT [GA]GCGC^[CT]
356 AGCGCC
HinfI 362 GAGTC G^A[ACGT]TC
658 GATTC
MaeII 209 ACGT A^CGT
434 ACGT
MflI 548 AGATCC [GA]^GATC[CT]
766 GGATCT
MslI 69 CACATCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
729 CATCTTCATG
NlaIII 345 CATG CATG^
735 CATG
PstI 10 CTGCAG CTGCA^G
352 CTGCAG
SfaNI 558 GATGC GCATC(5/9)
728 GCATC
SfcI 10 CTGCAG C^T[GA][CT]AG
352 CTGCAG
TspRI 35 CAGTG CA[GC]TG[ACGT][ACGT]^
299 CAGTG
XhoII 548 AGATCC [GA]^GATC[CT]
766 GGATCT
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# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AciI AluI AlwI BanII BbvI
BcnI BmyI BpmI BsaJI BseRI
BsiYI BslI Bsp1286I BspMI BssKI
BstNI CfoI Cfr13I DpnII EcoRII
Fnu4HI HaeIII HapII HhaI HinP1I
HpaII HphI ItaI MaeIII MboI
MnlI MspI MvaI MwoI NciI
NdeII NlaIV Sau3AI Sau96I ScrFI
TseI Tsp45I