Restriction map for
pFN21AB3440
(Linear) (Six-base) MAPSORT of: KIBB3440.seq Check: 1947 from: 1 to: 774
KIBB3440.orf
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 604 774
Size: 604 170
AccI GT'mk_AC
Cuts at: 0 590 774
Size: 590 184
AfeI AGC'GCT
Cuts at: 0 154 774
Size: 154 620
AflIII A'CryG_T
Cuts at: 0 134 774
Size: 134 640
AleI CACnn'nnGTG
Cuts at: 0 73 774
Size: 73 701
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 35 751 774
Size: 35 716 23
Fragments arranged by size:
716 35 23
AlwNI CAG_nnn'CTG
Cuts at: 0 552 774
Size: 552 222
ApaI G_GGCC'C
Cuts at: 0 344 404 774
Size: 344 60 370
Fragments arranged by size:
370 344 60
AvrII C'CTAG_G
Cuts at: 0 370 774
Size: 370 404
BanII G_rGCy'C
Cuts at: 0 344 404 710 774
Size: 344 60 306 64
Fragments arranged by size:
344 306 64 60
Bme1580I G_kGCm'C
Cuts at: 0 344 404 774
Size: 344 60 370
Fragments arranged by size:
370 344 60
BmgBI CAC'GTC
Cuts at: 0 210 774
Size: 210 564
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 80 181 304 774
Size: 80 101 123 470
Fragments arranged by size:
470 123 101 80
BsaAI yAC'GTr
Cuts at: 0 435 774
Size: 435 339
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 199 268 301 759 774
Size: 199 69 33 458 15
Fragments arranged by size:
458 199 69 33 15
BseYI C'CCAG_C
Cuts at: 0 237 333 499 575 688 702 774
Size: 237 96 166 76 113 14 72
Fragments arranged by size:
237 166 113 96 76 72 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 336 774
Size: 336 438
BsiHKAI G_wGCw'C
Cuts at: 0 611 774
Size: 611 163
BsmI GAATG_Cn'
Cuts at: 0 133 458 774
Size: 133 325 316
Fragments arranged by size:
325 316 133
Bsp1286I G_dGCh'C
Cuts at: 0 344 404 611 710 774
Size: 344 60 207 99 64
Fragments arranged by size:
344 207 99 64 60
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 3 428 604 774
Size: 3 425 176 170
Fragments arranged by size:
425 176 170 3
BstYI r'GATC_y
Cuts at: 0 548 766 774
Size: 548 218 8
Fragments arranged by size:
548 218 8
BtgI C'CryG_G
Cuts at: 0 635 774
Size: 635 139
BtsI GCAGTG_nn'
Cuts at: 0 305 774
Size: 305 469
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 80 181 304 774
Size: 80 101 123 470
Fragments arranged by size:
470 123 101 80
EcoNI CCTnn'n_nnAGG
Cuts at: 0 112 774
Size: 112 662
EcoO109I rG'GnC_Cy
Cuts at: 0 400 401 774
Size: 400 1 373
Fragments arranged by size:
400 373 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 420 774
Size: 420 354
HaeII r_GCGC'y
Cuts at: 0 156 360 774
Size: 156 204 414
Fragments arranged by size:
414 204 156
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 24 56 713 745 774
Size: 24 32 657 32 29
Fragments arranged by size:
657 32 32 29 24
MluI A'CGCG_T
Cuts at: 0 134 774
Size: 134 640
MslI CAynn'nnrTG
Cuts at: 0 73 733 774
Size: 73 660 41
Fragments arranged by size:
660 73 41
MspA1I CmG'CkG
Cuts at: 0 499 774
Size: 499 275
PflMI CCAn_nnn'nTGG
Cuts at: 0 552 774
Size: 552 222
PfoI T'CCnGG_A
Cuts at: 0 158 281 774
Size: 158 123 493
Fragments arranged by size:
493 158 123
PmlI CAC'GTG
Cuts at: 0 435 774
Size: 435 339
PspOMI G'GGCC_C
Cuts at: 0 340 400 774
Size: 340 60 374
Fragments arranged by size:
374 340 60
PstI C_TGCA'G
Cuts at: 0 14 356 774
Size: 14 342 418
Fragments arranged by size:
418 342 14
PvuII CAG'CTG
Cuts at: 0 499 774
Size: 499 275
ScaI AGT'ACT
Cuts at: 0 259 774
Size: 259 515
SfcI C'TryA_G
Cuts at: 0 10 352 774
Size: 10 342 422
Fragments arranged by size:
422 342 10
StyI C'CwwG_G
Cuts at: 0 370 774
Size: 370 404
TatI w'GTAC_w
Cuts at: 0 257 774
Size: 257 517
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 140 172 298 330 774
Size: 140 32 126 32 444
Fragments arranged by size:
444 140 126 32 32
Enzymes that do cut:
AarI AccI AfeI AflIII AleI AlfI AlwNI ApaI
AvrII BanII Bme1580I BmgBI BpmI BsaAI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspMI BstYI BtgI BtsI
Eco57MI EcoNI EcoO109I FalI HaeII Hin4I MluI MslI
MspA1I PflMI PfoI PmlI PspOMI PstI PvuII ScaI
SfcI StyI TatI TstI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AflII AgeI AhdI AloI
ApaLI ApoI AscI AseI AsiSI AvaI BaeI BamHI
BanI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI BmrI BmtI BplI Bpu10I BpuEI
BsaI BsaBI BsaHI BsaWI BsaXI BsiEI BsiWI BsmBI
BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI EcoRI EcoRV FseI FspI FspAI HincII HindIII
HpaI KasI KpnI MfeI MmeI MscI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PmeI PpiI PpuMI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII Tth111I XbaI XcmI XhoI XmaI XmnI ZraI