(Circular) (Six-base) MAPSORT of: pFN21AE4261.seq Check: 95 from: 1 to: 7876 pFN21AE4261 7876 bp With 182 enzymes: SgfI * March 28, 2012 13:45 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3436 3436 Size: 7876 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5686 278 Size: 53 83 186 936 4150 2468 Fragments arranged by size: 4150 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3061 5219 1937 Size: 1124 2158 4594 Fragments arranged by size: 4594 2158 1124 Acc65I G'GTAC_C Cuts at: 3039 3359 5197 3039 Size: 320 1838 5718 Fragments arranged by size: 5718 1838 320 AclI AA'CG_TT Cuts at: 1563 3290 6003 6376 1563 Size: 1727 2713 373 3063 Fragments arranged by size: 3063 2713 1727 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3099 3157 5149 5939 7323 7591 1632 Size: 1467 58 1992 790 1384 268 1917 Fragments arranged by size: 1992 1917 1467 1384 790 268 58 AfeI AGC'GCT Cuts at: 2298 2298 Size: 7876 AflII C'TTAA_G Cuts at: 820 1017 2555 820 Size: 197 1538 6141 Fragments arranged by size: 6141 1538 197 AflIII A'CryG_T Cuts at: 1203 2174 6775 7752 1203 Size: 971 4601 977 1327 Fragments arranged by size: 4601 1327 977 971 AgeI A'CCGG_T Cuts at: 2990 6707 2990 Size: 3717 4159 AhdI GACnn_n'nnGTC Cuts at: 6605 6605 Size: 7876 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7876 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2256 2940 1184 Size: 1072 684 6120 Fragments arranged by size: 6120 1072 684 AlwNI CAG_nnn'CTG Cuts at: 4549 4966 5619 7191 4549 Size: 417 653 1572 5234 Fragments arranged by size: 5234 1572 653 417 ApaLI G'TGCA_C Cuts at: 5933 7089 5933 Size: 1156 6720 ApoI r'AATT_y Cuts at: 1493 2949 3102 3756 3848 4271 4914 5185 5411 Size: 1456 153 654 92 423 643 271 226 Cuts at: 5411 5563 7672 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1456 654 643 423 271 226 153 152 92 AseI AT'TA_AT Cuts at: 160 1032 6430 160 Size: 872 5398 1606 Fragments arranged by size: 5398 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7876 AvaI C'yCGr_G Cuts at: 1870 1943 5201 1870 Size: 73 3258 4545 Fragments arranged by size: 4545 3258 73 BamHI G'GATC_C Cuts at: 4303 5206 5573 4303 Size: 903 367 6606 Fragments arranged by size: 6606 903 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3039 3359 3619 5197 6652 Size: 325 217 627 1252 320 260 1578 1455 Cuts at: 6652 618 Size: 1842 Fragments arranged by size: 1842 1578 1455 1252 627 325 320 260 217 BanII G_rGCy'C Cuts at: 1391 2079 2605 5195 1391 Size: 688 526 2590 4072 Fragments arranged by size: 4072 2590 688 526 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2470 4380 928 Size: 971 571 1910 4424 Fragments arranged by size: 4424 1910 971 571 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6067 6101 1903 Size: 34 4130 34 3678 Fragments arranged by size: 4130 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5768 6978 5768 Size: 1210 6666 BclI T'GATC_A Cuts at: 1548 3343 3424 4063 5149 1548 Size: 1795 81 639 1086 4275 Fragments arranged by size: 4275 1795 1086 639 81 BfrBI ATG'CAT Cuts at: 2024 2376 2024 Size: 352 7524 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6487 136 Size: 107 122 71 631 605 4815 1525 Fragments arranged by size: 4815 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2723 3198 7871 2723 Size: 475 4673 2728 Fragments arranged by size: 4673 2728 475 BlpI GC'TnA_GC Cuts at: 3236 4414 5293 3236 Size: 1178 879 5819 Fragments arranged by size: 5819 1178 879 Bme1580I G_kGCm'C Cuts at: 1790 5937 7093 1790 Size: 4147 1156 2573 Fragments arranged by size: 4147 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7876 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3913 4097 5057 Size: 416 53 83 399 24 2496 184 960 Cuts at: 5057 5607 6565 442 Size: 550 958 1753 Fragments arranged by size: 2496 1753 960 958 550 416 399 184 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7876 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2649 2829 6536 1239 Size: 1410 180 3707 2579 Fragments arranged by size: 3707 2579 1410 180 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3659 4202 6001 6866 7164 3659 Size: 543 1799 865 298 4371 Fragments arranged by size: 4371 1799 865 543 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2336 6539 882 Size: 617 837 4203 2219 Fragments arranged by size: 4203 2219 837 617 BsaAI yAC'GTr Cuts at: 493 1204 5667 493 Size: 711 4463 2702 Fragments arranged by size: 4463 2702 711 BsaBI GATnn'nnATC Cuts at: 7870 7870 Size: 7876 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5683 6065 6700 275 Size: 53 83 186 936 4150 382 635 1451 Fragments arranged by size: 4150 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2990 6309 6707 6981 7128 7539 2990 Size: 3319 398 274 147 411 3327 Fragments arranged by size: 3327 3319 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4770 4800 1178 Size: 30 165 30 3367 30 4254 Fragments arranged by size: 4254 3367 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2613 4078 5066 1186 Size: 1427 1465 988 3996 Fragments arranged by size: 3996 1465 1427 988 BseYI C'CCAG_C Cuts at: 2013 2983 7079 7575 2013 Size: 970 4096 496 2314 Fragments arranged by size: 4096 2314 970 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4674 1163 Size: 573 2938 4365 Fragments arranged by size: 4365 2938 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5326 6087 7115 1540 Size: 275 186 3325 761 1028 2301 Fragments arranged by size: 3325 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5937 6022 7093 5937 Size: 85 1071 6720 Fragments arranged by size: 6720 1071 85 BsmI GAATG_Cn' Cuts at: 2378 3391 4992 5387 5480 2378 Size: 1013 1601 395 93 4774 Fragments arranged by size: 4774 1601 1013 395 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2079 2605 5195 5937 6022 7093 1391 Size: 399 289 526 2590 742 85 1071 2174 Fragments arranged by size: 2590 2174 1071 742 526 399 289 85 BspEI T'CCGG_A Cuts at: 7539 7539 Size: 7876 BspHI T'CATG_A Cuts at: 2206 2845 4287 5765 7495 2206 Size: 639 1442 1478 1730 2587 Fragments arranged by size: 2587 1730 1478 1442 639 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2224 3436 5231 844 Size: 1380 1212 1795 3489 Fragments arranged by size: 3489 1795 1380 1212 BsrBI CCG'CTC Cuts at: 1394 1823 5763 1394 Size: 429 3940 3507 Fragments arranged by size: 3940 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3343 4176 4827 6371 6545 65 Size: 1183 2095 833 651 1544 174 1396 Fragments arranged by size: 2095 1544 1396 1183 833 651 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2990 6520 6707 1700 Size: 110 1180 3530 187 2869 Fragments arranged by size: 3530 2869 1180 187 110 BsrGI T'GTAC_A Cuts at: 96 2169 2422 3980 96 Size: 2073 253 1558 3992 Fragments arranged by size: 3992 2073 1558 253 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7876 BssSI C'ACGA_G Cuts at: 5936 6948 5936 Size: 1012 6864 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7876 BstBI TT'CG_AA Cuts at: 6680 6680 Size: 7876 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7876 BstXI CCAn_nnnn'nTGG Cuts at: 3155 4397 3155 Size: 1242 6634 BstYI r'GATC_y Cuts at: 1972 2723 3198 4303 5092 5206 5573 5958 5975 Size: 751 475 1105 789 114 367 385 17 Cuts at: 5975 7427 7513 7525 7871 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1105 789 751 475 385 367 346 114 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4181 4181 Size: 7876 BtgI C'CryG_G Cuts at: 513 1065 3940 513 Size: 552 2875 4449 Fragments arranged by size: 4449 2875 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7137 Fragments arranged by size: 7137 683 56 BtsI GCAGTG_nn' Cuts at: 3062 5392 6186 6206 3062 Size: 2330 794 20 4732 Fragments arranged by size: 4732 2330 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7876 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2767 2802 529 Size: 35 2203 35 5603 Fragments arranged by size: 5603 2203 35 35 DraI TTT'AAA Cuts at: 5180 5180 Size: 7876 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2202 6883 809 Size: 507 886 4681 1802 Fragments arranged by size: 4681 1802 886 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2503 5323 6212 Size: 54 1425 50 275 18 673 2820 889 Cuts at: 6212 7841 8 Size: 1629 43 Fragments arranged by size: 2820 1629 1425 889 673 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5323 1537 Size: 275 3511 4090 Fragments arranged by size: 4090 3511 275 EarI CTCTTCn'nnn_ Cuts at: 1353 5006 5806 1353 Size: 3653 800 3423 Fragments arranged by size: 3653 3423 800 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6463 6837 6983 387 Size: 1064 138 4874 374 146 1280 Fragments arranged by size: 4874 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2649 2829 3099 3157 5149 5939 6536 Size: 393 1017 180 270 58 1992 790 597 Cuts at: 6536 7323 7591 1239 Size: 787 268 1524 Fragments arranged by size: 1992 1524 1017 790 787 597 393 270 268 180 58 EcoNI CCTnn'n_nnAGG Cuts at: 2940 3614 2940 Size: 674 7202 EcoRI G'AATT_C Cuts at: 5185 5563 5185 Size: 378 7498 EcoRV GAT'ATC Cuts at: 2413 3438 3964 2413 Size: 1025 526 6325 Fragments arranged by size: 6325 1025 526 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4510 4510 Size: 7876 FspI TGC'GCA Cuts at: 4265 6382 4265 Size: 2117 5759 HaeII r_GCGC'y Cuts at: 1678 2300 7023 1678 Size: 622 4723 2531 Fragments arranged by size: 4723 2531 622 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2115 2147 3508 3540 4770 Size: 32 135 32 543 32 1361 32 1230 Cuts at: 4770 4802 6517 6549 6591 6623 1373 Size: 32 1715 32 42 32 2626 Fragments arranged by size: 2626 1715 1361 1230 543 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3937 4669 5220 5466 669 Size: 988 281 1999 732 551 246 3079 Fragments arranged by size: 3079 1999 988 732 551 281 246 HindIII A'AGCT_T Cuts at: 748 3124 748 Size: 2376 5500 HpaI GTT'AAC Cuts at: 4669 5466 4669 Size: 797 7079 KpnI G_GTAC'C Cuts at: 3043 3363 5201 3043 Size: 320 1838 5718 Fragments arranged by size: 5718 1838 320 MfeI C'AATT_G Cuts at: 2507 5475 2507 Size: 2968 4908 MluI A'CGCG_T Cuts at: 7752 7752 Size: 7876 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3212 3585 3934 4213 6990 7174 1305 Size: 1907 373 349 279 2777 184 2007 Fragments arranged by size: 2777 2007 1907 373 349 279 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2505 10 Size: 54 1425 343 673 5381 Fragments arranged by size: 5381 1425 673 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2179 5834 6193 6352 518 Size: 648 1013 3655 359 159 2042 Fragments arranged by size: 3655 2042 1013 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2551 2983 4664 5292 5969 7117 7362 1606 Size: 945 432 1681 628 677 1148 245 2120 Fragments arranged by size: 2120 1681 1148 945 677 628 432 245 NcoI C'CATG_G Cuts at: 513 1065 3940 513 Size: 552 2875 4449 Fragments arranged by size: 4449 2875 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7876 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7876 NotI GC'GGCC_GC Cuts at: 5323 5323 Size: 7876 NsiI A_TGCA'T Cuts at: 2026 2378 2026 Size: 352 7524 NspI r_CATG'y Cuts at: 1132 1229 2178 4321 4540 5234 6779 1132 Size: 97 949 2143 219 694 1545 2229 Fragments arranged by size: 2229 2143 1545 949 694 219 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7876 PciI A'CATG_T Cuts at: 2174 6775 2174 Size: 4601 3275 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7876 PmeI GTTT'AAAC Cuts at: 5180 5180 Size: 7876 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5945 5977 7458 7490 5945 Size: 32 1481 32 6331 Fragments arranged by size: 6331 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7876 PsiI TTA'TAA Cuts at: 2099 4425 5446 2099 Size: 2326 1021 4529 Fragments arranged by size: 4529 2326 1021 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4187 4219 4187 Size: 32 7844 PstI C_TGCA'G Cuts at: 830 5228 830 Size: 4398 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7876 PvuII CAG'CTG Cuts at: 2551 2983 4664 2551 Size: 432 1681 5763 Fragments arranged by size: 5763 1681 432 SalI G'TCGA_C Cuts at: 1936 5218 1936 Size: 3282 4594 SbfI CC_TGCA'GG Cuts at: 5228 5228 Size: 7876 ScaI AGT'ACT Cuts at: 3633 3654 6124 7836 3633 Size: 21 2470 1712 3673 Fragments arranged by size: 3673 2470 1712 21 SfcI C'TryA_G Cuts at: 826 1045 3349 5224 6359 7040 7231 826 Size: 219 2304 1875 1135 681 191 1471 Fragments arranged by size: 2304 1875 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7876 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7876 SmaI CCC'GGG Cuts at: 1872 5203 1872 Size: 3331 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2555 3674 4217 5980 6881 7143 Size: 197 926 612 1119 543 1763 901 262 Cuts at: 7143 820 Size: 1553 Fragments arranged by size: 1763 1553 1119 926 901 612 543 262 197 SnaBI TAC'GTA Cuts at: 493 5667 493 Size: 5174 2702 SpeI A'CTAG_T Cuts at: 152 5023 152 Size: 4871 3005 SphI G_CATG'C Cuts at: 1132 4540 5234 1132 Size: 3408 694 3774 Fragments arranged by size: 3774 3408 694 SspI AAT'ATT Cuts at: 5 52 2260 2395 3086 4156 4889 5800 5 Size: 47 2208 135 691 1070 733 911 2081 Fragments arranged by size: 2208 2081 1070 911 733 691 135 47 StuI AGG'CCT Cuts at: 1517 3920 1517 Size: 2403 5473 StyI C'CwwG_G Cuts at: 513 1065 2778 2858 3940 4494 5315 513 Size: 552 1713 80 1082 554 821 3074 Fragments arranged by size: 3074 1713 1082 821 554 552 80 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2036 4046 5903 5920 6073 6258 6703 Size: 1255 456 2010 1857 17 153 185 445 Cuts at: 6703 325 Size: 1498 Fragments arranged by size: 2010 1857 1498 1255 456 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2068 2169 2422 Size: 274 80 33 51 1443 91 101 253 Cuts at: 2422 2614 3631 3652 3865 3980 6122 7834 96 Size: 192 1017 21 213 115 2142 1712 138 Fragments arranged by size: 2142 1712 1443 1017 274 253 213 192 138 115 101 91 80 51 33 21 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7836 7868 7836 Size: 32 7844 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7876 XbaI T'CTAG_A Cuts at: 5212 5212 Size: 7876 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7876 XmaI C'CCGG_G Cuts at: 1870 5201 1870 Size: 3331 4545 XmnI GAAnn'nnTTC Cuts at: 2992 3292 4929 6005 2992 Size: 300 1637 1076 4863 Fragments arranged by size: 4863 1637 1076 300 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5684 276 Size: 53 83 186 936 4150 2468 Fragments arranged by size: 4150 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI