(Linear) (Six-base) MAPSORT of: KIEE4261.seq Check: 62 from: 1 to: 3171 KIEE4261.orf With 182 enzymes: SgfI * March 22, 2012 17:39 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1431 3171 Size: 1431 1740 AccI GT'mk_AC Cuts at: 0 1056 3171 Size: 1056 2115 Acc65I G'GTAC_C Cuts at: 0 1034 1354 3171 Size: 1034 320 1817 Fragments arranged by size: 1817 1034 320 AclI AA'CG_TT Cuts at: 0 1285 3171 Size: 1285 1886 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1094 1152 3144 3171 Size: 1094 58 1992 27 Fragments arranged by size: 1992 1094 58 27 AfeI AGC'GCT Cuts at: 0 293 3171 Size: 293 2878 AflII C'TTAA_G Cuts at: 0 550 3171 Size: 550 2621 AflIII A'CryG_T Cuts at: 0 169 3171 Size: 169 3002 AgeI A'CCGG_T Cuts at: 0 985 3171 Size: 985 2186 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 251 935 3171 Size: 251 684 2236 Fragments arranged by size: 2236 684 251 AlwNI CAG_nnn'CTG Cuts at: 0 2544 2961 3171 Size: 2544 417 210 Fragments arranged by size: 2544 417 210 ApoI r'AATT_y Cuts at: 0 944 1097 1751 1843 2266 2909 3171 Size: 944 153 654 92 423 643 262 Fragments arranged by size: 944 654 643 423 262 153 92 BamHI G'GATC_C Cuts at: 0 2298 3171 Size: 2298 873 BanI G'GyrC_C Cuts at: 0 1034 1354 1614 3171 Size: 1034 320 260 1557 Fragments arranged by size: 1557 1034 320 260 BanII G_rGCy'C Cuts at: 0 74 600 3171 Size: 74 526 2571 Fragments arranged by size: 2571 526 74 BbsI GAAGACnn'nnnn_ Cuts at: 0 465 2375 3171 Size: 465 1910 796 Fragments arranged by size: 1910 796 465 BclI T'GATC_A Cuts at: 0 1338 1419 2058 3144 3171 Size: 1338 81 639 1086 27 Fragments arranged by size: 1338 1086 639 81 27 BfrBI ATG'CAT Cuts at: 0 19 371 3171 Size: 19 352 2800 Fragments arranged by size: 2800 352 19 BglII A'GATC_T Cuts at: 0 718 1193 3171 Size: 718 475 1978 Fragments arranged by size: 1978 718 475 BlpI GC'TnA_GC Cuts at: 0 1231 2409 3171 Size: 1231 1178 762 Fragments arranged by size: 1231 1178 762 BmrI ACTGGGnnnn_n' Cuts at: 0 1908 2092 3052 3171 Size: 1908 184 960 119 Fragments arranged by size: 1908 960 184 119 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 644 824 3171 Size: 644 180 2347 Fragments arranged by size: 2347 644 180 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1654 2197 3171 Size: 1654 543 974 Fragments arranged by size: 1654 974 543 BsaI GGTCTCn'nnnn_ Cuts at: 0 331 3171 Size: 331 2840 BsaWI w'CCGG_w Cuts at: 0 985 3171 Size: 985 2186 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 2765 2795 3171 Size: 2765 30 376 Fragments arranged by size: 2765 376 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 608 2073 3061 3171 Size: 608 1465 988 110 Fragments arranged by size: 1465 988 608 110 BseYI C'CCAG_C Cuts at: 0 8 978 3171 Size: 8 970 2193 Fragments arranged by size: 2193 970 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2669 3171 Size: 2669 502 BsmI GAATG_Cn' Cuts at: 0 373 1386 2987 3171 Size: 373 1013 1601 184 Fragments arranged by size: 1601 1013 373 184 Bsp1286I G_dGCh'C Cuts at: 0 74 600 3171 Size: 74 526 2571 Fragments arranged by size: 2571 526 74 BspHI T'CATG_A Cuts at: 0 201 840 2282 3171 Size: 201 639 1442 889 Fragments arranged by size: 1442 889 639 201 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 219 1431 3171 Size: 219 1212 1740 Fragments arranged by size: 1740 1212 219 BsrDI GCAATG_nn' Cuts at: 0 1338 2171 2822 3171 Size: 1338 833 651 349 Fragments arranged by size: 1338 833 651 349 BsrFI r'CCGG_y Cuts at: 0 985 3171 Size: 985 2186 BsrGI T'GTAC_A Cuts at: 0 164 417 1975 3171 Size: 164 253 1558 1196 Fragments arranged by size: 1558 1196 253 164 BstXI CCAn_nnnn'nTGG Cuts at: 0 1150 2392 3171 Size: 1150 1242 779 Fragments arranged by size: 1242 1150 779 BstYI r'GATC_y Cuts at: 0 718 1193 2298 3087 3171 Size: 718 475 1105 789 84 Fragments arranged by size: 1105 789 718 475 84 Bsu36I CC'TnA_GG Cuts at: 0 2176 3171 Size: 2176 995 BtgI C'CryG_G Cuts at: 0 1935 3171 Size: 1935 1236 BtsI GCAGTG_nn' Cuts at: 0 1057 3171 Size: 1057 2114 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 762 797 3171 Size: 762 35 2374 Fragments arranged by size: 2374 762 35 DrdI GACnn_nn'nnGTC Cuts at: 0 197 3171 Size: 197 2974 EaeI y'GGCC_r Cuts at: 0 498 3171 Size: 498 2673 EarI CTCTTCn'nnn_ Cuts at: 0 3001 3171 Size: 3001 170 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 644 824 1094 1152 3144 3171 Size: 644 180 270 58 1992 27 Fragments arranged by size: 1992 644 270 180 58 27 EcoNI CCTnn'n_nnAGG Cuts at: 0 935 1609 3171 Size: 935 674 1562 Fragments arranged by size: 1562 935 674 EcoRV GAT'ATC Cuts at: 0 408 1433 1959 3171 Size: 408 1025 526 1212 Fragments arranged by size: 1212 1025 526 408 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 2505 3171 Size: 2505 666 FspI TGC'GCA Cuts at: 0 2260 3171 Size: 2260 911 HaeII r_GCGC'y Cuts at: 0 295 3171 Size: 295 2876 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 110 142 1503 1535 2765 2797 3171 Size: 110 32 1361 32 1230 32 374 Fragments arranged by size: 1361 1230 374 110 32 32 32 HincII GTy'rAC Cuts at: 0 1932 2664 3171 Size: 1932 732 507 Fragments arranged by size: 1932 732 507 HindIII A'AGCT_T Cuts at: 0 1119 3171 Size: 1119 2052 HpaI GTT'AAC Cuts at: 0 2664 3171 Size: 2664 507 KpnI G_GTAC'C Cuts at: 0 1038 1358 3171 Size: 1038 320 1813 Fragments arranged by size: 1813 1038 320 MfeI C'AATT_G Cuts at: 0 502 3171 Size: 502 2669 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1207 1580 1929 2208 3171 Size: 1207 373 349 279 963 Fragments arranged by size: 1207 963 373 349 279 MscI TGG'CCA Cuts at: 0 500 3171 Size: 500 2671 MslI CAynn'nnrTG Cuts at: 0 174 3171 Size: 174 2997 MspA1I CmG'CkG Cuts at: 0 546 978 2659 3171 Size: 546 432 1681 512 Fragments arranged by size: 1681 546 512 432 NcoI C'CATG_G Cuts at: 0 1935 3171 Size: 1935 1236 NsiI A_TGCA'T Cuts at: 0 21 373 3171 Size: 21 352 2798 Fragments arranged by size: 2798 352 21 NspI r_CATG'y Cuts at: 0 173 2316 2535 3171 Size: 173 2143 219 636 Fragments arranged by size: 2143 636 219 173 PciI A'CATG_T Cuts at: 0 169 3171 Size: 169 3002 PsiI TTA'TAA Cuts at: 0 94 2420 3171 Size: 94 2326 751 Fragments arranged by size: 2326 751 94 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 2182 2214 3171 Size: 2182 32 957 Fragments arranged by size: 2182 957 32 PvuII CAG'CTG Cuts at: 0 546 978 2659 3171 Size: 546 432 1681 512 Fragments arranged by size: 1681 546 512 432 ScaI AGT'ACT Cuts at: 0 1628 1649 3171 Size: 1628 21 1522 Fragments arranged by size: 1628 1522 21 SfcI C'TryA_G Cuts at: 0 1344 3171 Size: 1344 1827 SmlI C'TyrA_G Cuts at: 0 550 1669 2212 3171 Size: 550 1119 543 959 Fragments arranged by size: 1119 959 550 543 SpeI A'CTAG_T Cuts at: 0 3018 3171 Size: 3018 153 SphI G_CATG'C Cuts at: 0 2535 3171 Size: 2535 636 SspI AAT'ATT Cuts at: 0 255 390 1081 2151 2884 3171 Size: 255 135 691 1070 733 287 Fragments arranged by size: 1070 733 691 287 255 135 StuI AGG'CCT Cuts at: 0 1915 3171 Size: 1915 1256 StyI C'CwwG_G Cuts at: 0 773 853 1935 2489 3171 Size: 773 80 1082 554 682 Fragments arranged by size: 1082 773 682 554 80 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 31 2041 3171 Size: 31 2010 1130 Fragments arranged by size: 2010 1130 31 TatI w'GTAC_w Cuts at: 0 63 164 417 609 1626 1647 1860 1975 Size: 63 101 253 192 1017 21 213 115 Cuts at: 1975 3171 Size: 1196 Fragments arranged by size: 1196 1017 253 213 192 115 101 63 21 XmnI GAAnn'nnTTC Cuts at: 0 987 1287 2924 3171 Size: 987 300 1637 247 Fragments arranged by size: 1637 987 300 247 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AlfI AlwNI ApoI BamHI BanI BanII BbsI BclI BfrBI BglII BlpI BmrI BpmI BpuEI BsaI BsaWI BsaXI BseRI BseYI BsgI BsmI Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI BstXI BstYI Bsu36I BtgI BtsI CspCI DrdI EaeI EarI Eco57MI EcoNI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MmeI MscI MslI MspA1I NcoI NsiI NspI PciI PsiI PsrI PvuII ScaI SfcI SmlI SpeI SphI SspI StuI StyI TaqII TatI XmnI Enzymes that do not cut: AatII AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BbeI BbvCI BcgI BciVI BglI Bme1580I BmgBI BmtI BplI Bpu10I BsaAI BsaBI BsaHI BsiEI BsiHKAI BsiWI BsmBI BspEI BsrBI BssHII BssSI BstAPI BstBI BstEII BstZ17I BtgZI ClaI DraI DraIII EagI EciI EcoICRI EcoO109I EcoRI FseI FspAI KasI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PasI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PstI PvuI RsrII SacI SacII SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SrfI SwaI TstI Tth111I XbaI XcmI XhoI XmaI ZraI