################################## # NO CUT ENZYMES ( 104 enzymes) # ################################## AatII Acc65I AccIII AclI AflIII AgeI AocI Aor51HI AscI AseI AsnI Asp718I AspI AviII BamHI BbrPI BclI BlpI BpmI Bpu1102I BsaBI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BspEI BspLU11I BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstBI BstZ17I Bsu36I CelII Cfr10I CpoI DraI DraIII DrdI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoT22I FbaI FseI FspI HgaI HindIII HpaI KpnI KspI MamI MluI MroI NaeI NdeI NgoMIV NheI NotI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspI StuI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AccI 1378 GTAGAC GT^[AC][GT]AC AccII 94 CGCG CG^CG AflII 2311 CTTAAG C^TTAAG AhdI 913 GACCTGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1810 GTGCAC G^TGCAC AlwNI 112 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 600 GGGCCC GGGCC^C ApaLI 1810 GTGCAC G^TGCAC AspEI 913 GACCTGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 70 CCCGAG C^[CT]CG[GA]G AvrII 804 CCTAGG C^CTAGG BalI 561 TGGCCA TGG^CCA BbvCI 1031 CCTCAGC CCTCAGC(-5/-2) BcgI 1885 CGATGGTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1161 GGATAC GTATCC(6/5) BcnI 77 CCGGG CC^[GC]GG BfrI 2311 CTTAAG C^TTAAG BglI 116 GCCTGCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 804 CCTAGG C^CTAGG BsaAI 955 TACGTG [CT]AC^GT[GA] BsaI 920 GGTCTC GGTCTC(1/5) BsgI 1105 CTGCAC GTGCAG(16/14) BsoBI 70 CCCGAG C^[CT]CG[GA]G Bsp1407I 522 TGTACA T^GTACA BspDI 1312 ATCGAT AT^CGAT BspHI 2472 TCATGA T^CATGA BspMI 2227 GCAGGT ACCTGC(4/8) BsrGI 522 TGTACA T^GTACA BstAPI 111 GCAGAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 684 GGTAACC G^GT[ACGT]ACC BstPI 684 GGTAACC G^GT[ACGT]ACC BstUI 94 CGCG CG^CG ClaI 1312 ATCGAT AT^CGAT DraII 600 GGGCCCT [GA]G^G[ACGT]CC[CT] DsaI 540 CCATGG C^C[GA][CT]GG EaeI 561 TGGCCA [CT]^GGCC[GA] Eam1105I 913 GACCTGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO109I 600 GGGCCCT [GA]G^G[ACGT]CC[CT] EcoO65I 684 GGTAACC G^GT[ACGT]ACC EcoRV 1438 GATATC GAT^ATC FauI 120 GCGGG CCCGC(4/6) HapII 77 CCGG C^CGG HincII 427 GTTGAC GT[CT]^[GA]AC HindII 427 GTTGAC GT[CT]^[GA]AC HpaII 77 CCGG C^CGG MluNI 561 TGGCCA TGG^CCA MscI 561 TGGCCA TGG^CCA MspI 77 CCGG C^CGG MvnI 94 CGCG CG^CG NciI 77 CCGGG CC^[GC]GG NcoI 540 CCATGG C^CATGG NruI 93 TCGCGA TCG^CGA NspI 535 GCATGC [GA]CATG^[CT] PvuII 1034 CAGCTG CAG^CTG RcaI 2472 TCATGA T^CATGA ScaI 971 AGTACT AGT^ACT SphI 535 GCATGC GCATG^C SspBI 522 TGTACA T^GTACA ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AcyI 12 GGCGCC G[GA]^CG[CT]C 42 GGCGCC Asp700I 839 GAACTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2282 GAATTTATTC BanI 12 GGCGCC G^G[CT][GA]CC 42 GGCGCC BbeI 12 GGCGCC GGCGC^C 42 GGCGCC BglII 128 AGATCT A^GATCT 342 AGATCT BsaHI 12 GGCGCC G[GA]^CG[CT]C 42 GGCGCC BseRI 1293 CTCCTC GAGGAG(10/8) 733 GAGGAG BstXI 194 CCATAAATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1133 CCACTGCTGTGG EcoT14I 540 CCATGG C^C[AT][AT]GG 804 CCTAGG HaeII 12 GGCGCC [GA]GCGC^[CT] 42 GGCGCC Hin1I 12 GGCGCC G[GA]^CG[CT]C 42 GGCGCC KasI 12 GGCGCC G^GCGCC 42 GGCGCC MfeI 1397 CAATTG C^AATTG 2531 CAATTG MspA1I 47 CAGCGG C[AC]G^C[GT]G 1034 CAGCTG MunI 1397 CAATTG C^AATTG 2531 CAATTG NarI 12 GGCGCC GG^CGCC 42 GGCGCC SfoI 12 GGCGCC GGC^GCC 42 GGCGCC SmlI 1844 CTCAAG C^T[CT][GA]AG 2311 CTTAAG StyI 540 CCATGG C^C[AT][AT]GG 804 CCTAGG Tsp45I 572 GTGAC ^GT[GC]AC 1289 GTGAC XmnI 839 GAACTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2282 GAATTTATTC ######################################### # 3 OR MORE CUT ENZYMES ( 75 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AspHI AvaII BanII BbsI BbvI BfaI BmyI BpuAI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII EarI EcoRII Fnu4HI FokI HaeIII HhaI HinP1I HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MvaI MwoI NdeII NlaIII NlaIV PflMI PleI PstI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I Van91I XhoII