(Circular) (Six-base) MAPSORT of: pFN21AE4233.seq Check: 570 from: 1 to: 7255 pFN21AE4233 7255 bp With 182 enzymes: SgfI * March 28, 2012 13:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4223 4223 Size: 7255 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5065 278 Size: 53 83 186 936 3529 2468 Fragments arranged by size: 3529 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3384 4598 1937 Size: 1447 1214 4594 Fragments arranged by size: 4594 1447 1214 Acc65I G'GTAC_C Cuts at: 4576 4576 Size: 7255 AclI AA'CG_TT Cuts at: 1563 5382 5755 1563 Size: 3819 373 3063 Fragments arranged by size: 3819 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2955 5318 6702 6970 1632 Size: 1323 2363 1384 268 1917 Fragments arranged by size: 2363 1917 1384 1323 268 AflII C'TTAA_G Cuts at: 820 1017 4316 820 Size: 197 3299 3759 Fragments arranged by size: 3759 3299 197 AflIII A'CryG_T Cuts at: 1203 6154 7131 1203 Size: 4951 977 1327 Fragments arranged by size: 4951 1327 977 AgeI A'CCGG_T Cuts at: 6086 6086 Size: 7255 AhdI GACnn_n'nnGTC Cuts at: 2923 5984 2923 Size: 3061 4194 AleI CACnn'nnGTG Cuts at: 1166 3143 1166 Size: 1977 5278 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7255 AlwNI CAG_nnn'CTG Cuts at: 2122 4998 6570 2122 Size: 2876 1572 2807 Fragments arranged by size: 2876 2807 1572 ApaI G_GGCC'C Cuts at: 2609 2609 Size: 7255 ApaLI G'TGCA_C Cuts at: 3815 5312 6468 3815 Size: 1497 1156 4602 Fragments arranged by size: 4602 1497 1156 ApoI r'AATT_y Cuts at: 1493 2149 2203 2273 3199 3707 3768 3877 4287 Size: 656 54 70 926 508 61 109 410 Cuts at: 4287 4564 4790 4942 7051 1493 Size: 277 226 152 2109 1697 Fragments arranged by size: 2109 1697 926 656 508 410 277 226 152 109 70 61 54 AseI AT'TA_AT Cuts at: 160 1032 5809 160 Size: 872 4777 1606 Fragments arranged by size: 4777 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7255 AvaI C'yCGr_G Cuts at: 1870 1943 2075 4580 1870 Size: 73 132 2505 4545 Fragments arranged by size: 4545 2505 132 73 AvrII C'CTAG_G Cuts at: 2809 2809 Size: 7255 BamHI G'GATC_C Cuts at: 4585 4952 4585 Size: 367 6888 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2017 2047 4576 6031 618 Size: 325 217 627 230 30 2529 1455 1842 Fragments arranged by size: 2529 1842 1455 627 325 230 217 30 BanII G_rGCy'C Cuts at: 1391 2076 2609 3850 3918 4007 4574 1391 Size: 685 533 1241 68 89 567 4072 Fragments arranged by size: 4072 1241 685 567 533 89 68 BbeI G_GCGC'C Cuts at: 2021 2051 2021 Size: 30 7225 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2388 2636 2961 4275 928 Size: 971 489 248 325 1314 3908 Fragments arranged by size: 3908 1314 971 489 325 248 BbvCI CC'TCA_GC Cuts at: 3037 3037 Size: 7255 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3879 3913 5446 5480 1903 Size: 34 1942 34 1533 34 3678 Fragments arranged by size: 3678 1942 1533 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3160 5147 6357 3160 Size: 1987 1210 4058 Fragments arranged by size: 4058 1987 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7255 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2127 5866 Size: 107 122 71 631 605 337 118 3739 Cuts at: 5866 136 Size: 1525 Fragments arranged by size: 3739 1525 631 605 337 122 118 107 71 BglII A'GATC_T Cuts at: 2133 2347 7250 2133 Size: 214 4903 2138 Fragments arranged by size: 4903 2138 214 BlpI GC'TnA_GC Cuts at: 4672 4672 Size: 7255 Bme1580I G_kGCm'C Cuts at: 1790 2609 3252 3819 5316 6472 1790 Size: 819 643 567 1497 1156 2573 Fragments arranged by size: 2573 1497 1156 819 643 567 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7255 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2716 3959 4986 Size: 416 53 83 399 24 1299 1243 1027 Cuts at: 4986 5944 442 Size: 958 1753 Fragments arranged by size: 1753 1299 1243 1027 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7255 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5915 1239 Size: 4676 2579 Bpu10I CC'TnA_GC Cuts at: 3037 3037 Size: 7255 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3834 5380 6245 6543 3834 Size: 1546 865 298 4546 Fragments arranged by size: 4546 1546 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2931 5918 882 Size: 617 1432 2987 2219 Fragments arranged by size: 2987 2219 1432 617 BsaAI yAC'GTr Cuts at: 493 1204 2962 5046 493 Size: 711 1758 2084 2702 Fragments arranged by size: 2702 2084 1758 711 BsaBI GATnn'nnATC Cuts at: 7249 7249 Size: 7255 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2018 2048 5062 5444 Size: 53 83 186 936 485 30 3014 382 Cuts at: 5444 6079 275 Size: 635 1451 Fragments arranged by size: 3014 1451 936 635 485 382 186 83 53 30 BsaWI w'CCGG_w Cuts at: 5688 6086 6360 6507 6918 5688 Size: 398 274 147 411 6025 Fragments arranged by size: 6025 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3333 3363 1178 Size: 30 165 30 1930 30 5070 Fragments arranged by size: 5070 1930 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2753 3289 1186 Size: 1567 536 5152 Fragments arranged by size: 5152 1567 536 BseYI C'CCAG_C Cuts at: 3654 6458 6954 3654 Size: 2804 496 3955 Fragments arranged by size: 3955 2804 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3095 1163 Size: 573 1359 5323 Fragments arranged by size: 5323 1359 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4705 5466 6494 1540 Size: 275 186 2704 761 1028 2301 Fragments arranged by size: 2704 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3819 3850 3918 4007 5316 5401 6472 3819 Size: 31 68 89 1309 85 1071 4602 Fragments arranged by size: 4602 1309 1071 89 85 68 31 BsmI GAATG_Cn' Cuts at: 4766 4859 4766 Size: 93 7162 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2076 2609 3252 3819 3850 3918 4007 Size: 399 286 533 643 567 31 68 89 Cuts at: 4007 4574 5316 5401 6472 1391 Size: 567 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 643 567 567 533 399 286 89 85 68 31 BspEI T'CCGG_A Cuts at: 6918 6918 Size: 7255 BspHI T'CATG_A Cuts at: 4477 5144 6874 4477 Size: 667 1730 4858 Fragments arranged by size: 4858 1730 667 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4223 4610 844 Size: 3379 387 3489 Fragments arranged by size: 3489 3379 387 BsrBI CCG'CTC Cuts at: 1394 1823 5142 1394 Size: 429 3319 3507 Fragments arranged by size: 3507 3319 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5750 5924 65 Size: 1183 4502 174 1396 Fragments arranged by size: 4502 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5899 6086 1700 Size: 110 4089 187 2869 Fragments arranged by size: 4089 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2527 96 Size: 2431 4824 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7255 BssSI C'ACGA_G Cuts at: 5315 6327 5315 Size: 1012 6243 BstAPI GCAn_nnn'nTGC Cuts at: 794 2122 794 Size: 1328 5927 BstBI TT'CG_AA Cuts at: 6059 6059 Size: 7255 BstEII G'GTnAC_C Cuts at: 1184 2689 1184 Size: 1505 5750 BstXI CCAn_nnnn'nTGG Cuts at: 2206 3145 2206 Size: 939 6316 BstYI r'GATC_y Cuts at: 1972 2133 2347 2696 4499 4585 4952 5337 5354 Size: 161 214 349 1803 86 367 385 17 Cuts at: 5354 6806 6892 6904 7250 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1803 1452 385 367 349 346 214 161 86 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2545 513 Size: 552 939 541 5223 Fragments arranged by size: 5223 939 552 541 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6516 Fragments arranged by size: 6516 683 56 BtsI GCAGTG_nn' Cuts at: 3138 3519 4771 5565 5585 3138 Size: 381 1252 794 20 4808 Fragments arranged by size: 4808 1252 794 381 20 ClaI AT'CG_AT Cuts at: 1554 3318 1554 Size: 1764 5491 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7220 DraI TTT'AAA Cuts at: 4559 4559 Size: 7255 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6262 809 Size: 507 4946 1802 Fragments arranged by size: 4946 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2566 4702 5591 Size: 54 1425 50 275 18 736 2136 889 Cuts at: 5591 7220 8 Size: 1629 43 Fragments arranged by size: 2136 1629 1425 889 736 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4702 1537 Size: 275 2890 4090 Fragments arranged by size: 4090 2890 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2730 2934 3993 4329 5185 1353 Size: 1377 204 1059 336 856 3423 Fragments arranged by size: 3423 1377 1059 856 336 204 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5842 6216 6362 387 Size: 1064 138 4253 374 146 1280 Fragments arranged by size: 4253 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3848 3916 4005 3848 Size: 68 89 7098 Fragments arranged by size: 7098 89 68 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2955 5318 5915 6702 6970 1239 Size: 393 1323 2363 597 787 268 1524 Fragments arranged by size: 2363 1524 1323 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2606 2606 Size: 7255 EcoRI G'AATT_C Cuts at: 4564 4942 4564 Size: 378 6877 EcoRV GAT'ATC Cuts at: 3445 3445 Size: 7255 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2483 2483 Size: 7255 FspI TGC'GCA Cuts at: 5761 5761 Size: 7255 HaeII r_GCGC'y Cuts at: 1678 2021 2051 6402 1678 Size: 343 30 4351 2531 Fragments arranged by size: 4351 2531 343 30 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2909 2941 3457 3489 5896 Size: 32 135 32 1337 32 516 32 2407 Cuts at: 5896 5928 5970 6002 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2407 1337 516 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2434 4599 4845 669 Size: 988 281 496 2165 246 3079 Fragments arranged by size: 3079 2165 988 496 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7255 HpaI GTT'AAC Cuts at: 4845 4845 Size: 7255 KasI G'GCGC_C Cuts at: 2017 2047 2017 Size: 30 7225 KpnI G_GTAC'C Cuts at: 4580 4580 Size: 7255 MfeI C'AATT_G Cuts at: 3402 4536 4854 3402 Size: 1134 318 5803 Fragments arranged by size: 5803 1134 318 MluI A'CGCG_T Cuts at: 7131 7131 Size: 7255 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6369 6553 1305 Size: 5064 184 2007 Fragments arranged by size: 5064 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2568 10 Size: 54 1425 343 736 4697 Fragments arranged by size: 4697 1425 736 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3143 3434 3594 3700 5213 5572 5731 Size: 648 1977 291 160 106 1513 359 159 Cuts at: 5731 518 Size: 2042 Fragments arranged by size: 2042 1977 1513 648 359 291 160 159 106 MspA1I CmG'CkG Cuts at: 1606 2054 3041 4671 5348 6496 6741 1606 Size: 448 987 1630 677 1148 245 2120 Fragments arranged by size: 2120 1630 1148 987 677 448 245 NarI GG'CG_CC Cuts at: 2018 2048 2018 Size: 30 7225 NcoI C'CATG_G Cuts at: 513 1065 2004 2545 513 Size: 552 939 541 5223 Fragments arranged by size: 5223 939 552 541 NdeI CA'TA_TG Cuts at: 387 387 Size: 7255 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7255 NotI GC'GGCC_GC Cuts at: 4702 4702 Size: 7255 NruI TCG'CGA Cuts at: 2100 2100 Size: 7255 NspI r_CATG'y Cuts at: 1132 1229 2544 4613 6158 1132 Size: 97 1315 2069 1545 2229 Fragments arranged by size: 2229 2069 1545 1315 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7255 PciI A'CATG_T Cuts at: 6154 6154 Size: 7255 PflMI CCAn_nnn'nTGG Cuts at: 1108 2165 2239 4541 1108 Size: 1057 74 2302 3822 Fragments arranged by size: 3822 2302 1057 74 PmeI GTTT'AAAC Cuts at: 4559 4559 Size: 7255 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3604 3636 5324 5356 6837 6869 3604 Size: 32 1688 32 1481 32 3990 Fragments arranged by size: 3990 1688 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7255 PsiI TTA'TAA Cuts at: 4825 4825 Size: 7255 PspOMI G'GGCC_C Cuts at: 2605 2605 Size: 7255 PstI C_TGCA'G Cuts at: 830 2071 3514 4112 4234 4246 4326 4607 830 Size: 1241 1443 598 122 12 80 281 3478 Fragments arranged by size: 3478 1443 1241 598 281 122 80 12 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7255 PvuII CAG'CTG Cuts at: 3041 3041 Size: 7255 SacI G_AGCT'C Cuts at: 3850 3918 4007 3850 Size: 68 89 7098 Fragments arranged by size: 7098 89 68 SalI G'TCGA_C Cuts at: 1936 4597 1936 Size: 2661 4594 SbfI CC_TGCA'GG Cuts at: 4607 4607 Size: 7255 ScaI AGT'ACT Cuts at: 2978 5503 7215 2978 Size: 2525 1712 3018 Fragments arranged by size: 3018 2525 1712 SfcI C'TryA_G Cuts at: 826 1045 2067 2876 3510 3546 4108 4230 4242 Size: 219 1022 809 634 36 562 122 12 Cuts at: 4242 4322 4603 5738 6419 6610 826 Size: 80 281 1135 681 191 1471 Fragments arranged by size: 1471 1135 1022 809 681 634 562 281 219 191 122 80 36 12 SfoI GGC'GCC Cuts at: 2019 2049 2019 Size: 30 7225 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7255 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7255 SmaI CCC'GGG Cuts at: 1872 4582 1872 Size: 2710 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3849 4316 5359 6260 6522 820 Size: 197 926 1906 467 1043 901 262 1553 Fragments arranged by size: 1906 1553 1043 926 901 467 262 197 SnaBI TAC'GTA Cuts at: 493 5046 493 Size: 4553 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7255 SphI G_CATG'C Cuts at: 1132 2544 4613 1132 Size: 1412 2069 3774 Fragments arranged by size: 3774 2069 1412 SspI AAT'ATT Cuts at: 5 52 5179 5 Size: 47 5127 2081 Fragments arranged by size: 5127 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7255 StyI C'CwwG_G Cuts at: 513 1065 2004 2545 2809 4694 513 Size: 552 939 541 264 1885 3074 Fragments arranged by size: 3074 1885 939 552 541 264 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5282 5299 5452 5637 6082 325 Size: 1255 3702 17 153 185 445 1498 Fragments arranged by size: 3702 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2527 2976 4226 Size: 274 80 33 51 1443 550 449 1250 Cuts at: 4226 5501 7213 96 Size: 1275 1712 138 Fragments arranged by size: 1712 1443 1275 1250 550 449 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4044 4076 7215 7247 4044 Size: 32 3139 32 4052 Fragments arranged by size: 4052 3139 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7255 XbaI T'CTAG_A Cuts at: 4591 4591 Size: 7255 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7255 XmaI C'CCGG_G Cuts at: 1870 4580 1870 Size: 2710 4545 XmnI GAAnn'nnTTC Cuts at: 2848 4291 5384 2848 Size: 1443 1093 4719 Fragments arranged by size: 4719 1443 1093 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5063 276 Size: 53 83 186 936 3529 2468 Fragments arranged by size: 3529 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI FseI FspAI NaeI NgoMIV NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI